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The map label for this gene is purB [H]

Identifier: 154247772

GI number: 154247772

Start: 4265267

End: 4266574

Strand: Direct

Name: purB [H]

Synonym: Xaut_3849

Alternate gene names: 154247772

Gene position: 4265267-4266574 (Clockwise)

Preceding gene: 154247768

Following gene: 154247776

Centisome position: 80.34

GC content: 67.35

Gene sequence:

>1308_bases
ATGATCTCGCGTTACAGCCGGCCCGAAATGGCCGCCATCTGGTCCCCTGAAGCCCGCTTCCGCATCTGGTTCGAGATCGA
GGCCCATGCCGCCGACGCCATGGCGGACCTCGGCATCGTGCCGAAGGAGGCCGCGGCCAAGATCTGGGAGCTGGGCGGCA
AGGCCGAGTTCGACGTGGCGCGCATCGACGCCATCGAGCGGGACGTGAAGCACGACGTCATCGCCTTCCTCACCCATCTC
GCCGAGTTCGTGGGGCCGGAAGCCCGCTTCGTCCACCAGGGCATGACCTCATCCGACGTGCTGGACACCTGCCTCGCGGT
GCAGCTGTCGCGCGCCTGCGACCTGCTGATCGCCGACGTGGACGCGCTGCTGAAGGTGCTGGAGCGCCGGGCGTTCGAGT
TCAAGCTGACCCCCACCGTGGGCCGCTCCCACGGCATCCATGCCGAGCCCACCACCTTCGGCGTCAAGCTCGCCATCGCC
GCCGCCGAGTTCCAGCGCGCCCGCGCCCGGCTGGTCGCCGCCCGCGCCGAGATCGCCACCTGCGCCATTTCCGGGGCGGT
GGGCACGTTCGCCCATATCGACCCGGCGGTGGAAGCCCATGTGGCGCAAAAGCTCGGGCTCACCGTGGAGACCGTCTCCA
CCCAGGTGATCCCGCGCGACCGGCACGCCATGTTCTTCGCGGTGCTCGGCGTGGTCGCCTCCTCGGTGGAGCGGCTCGCC
ACCGAGGTGCGCCATCTCCAGCGCACCGAGGTGCTGGAGGCGGAGGAGTATTTCTCCCCCGAACAGAAGGGCTCCTCGGC
CATGCCCCACAAGCGCAACCCGGTGCTCACCGAGAACCTCACCGGCCTTGCCCGCATGGTGCGCGCCTATGCCCTGCCGG
CCATGGAGAACGTGGCCCTGTGGCACGAGCGGGACATCTCCCACTCCTCCGTGGAACGCATGATCGGCCCCGACGCCACG
GTGACGCTGGACTTCGCTTTGGCGCGGCTCACCGGCGTCATGGACAAGCTGGTGGTCCATACCGACAACATGCAGAAGAA
CATGGACAAGCTGGGCGGTCTCATCCACTCCCAGCGGGTGCTGCTGGCCCTGACCCAGAAGGGCGCGAGCCGCGAGGACA
GCTATCGGCTGGTGCAGCGCAACGCCATGCGGGTGTGGCACGGCGAGGGCGACTTCCGCACCTTCCTGCTGAACGACGCG
GACGTGCGCAAATACCTCTCGGAAGATGACATCAACGAGAAGTTCGATCTCGGCTACCACTTCAAGAACGTGGACAACAT
TTTCAAGCGGGTGTTCGGCCGGGCGTGA

Upstream 100 bases:

>100_bases
CGGCAAGCCCCATGTTCACGCCCCATACCTGCGCCGCGCTACCCGGCGCCGCGCCTTTCTGCTAAAGCGCTCGCCTGCCC
AATATGACGAGCACCCGCAC

Downstream 100 bases:

>100_bases
CCGGGGCCTGAACCGCCGTCGTGGCCGGGCTTGTCCCGGCCATTCATGCCCTGCGACCGGGAGGCGCCGAGGTCCCTCTC
CCGCTTGCGGGGGAGGGCAG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MISRYSRPEMAAIWSPEARFRIWFEIEAHAADAMADLGIVPKEAAAKIWELGGKAEFDVARIDAIERDVKHDVIAFLTHL
AEFVGPEARFVHQGMTSSDVLDTCLAVQLSRACDLLIADVDALLKVLERRAFEFKLTPTVGRSHGIHAEPTTFGVKLAIA
AAEFQRARARLVAARAEIATCAISGAVGTFAHIDPAVEAHVAQKLGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
TEVRHLQRTEVLEAEEYFSPEQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALARLTGVMDKLVVHTDNMQKNMDKLGGLIHSQRVLLALTQKGASREDSYRLVQRNAMRVWHGEGDFRTFLLNDA
DVRKYLSEDDINEKFDLGYHFKNVDNIFKRVFGRA

Sequences:

>Translated_435_residues
MISRYSRPEMAAIWSPEARFRIWFEIEAHAADAMADLGIVPKEAAAKIWELGGKAEFDVARIDAIERDVKHDVIAFLTHL
AEFVGPEARFVHQGMTSSDVLDTCLAVQLSRACDLLIADVDALLKVLERRAFEFKLTPTVGRSHGIHAEPTTFGVKLAIA
AAEFQRARARLVAARAEIATCAISGAVGTFAHIDPAVEAHVAQKLGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
TEVRHLQRTEVLEAEEYFSPEQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALARLTGVMDKLVVHTDNMQKNMDKLGGLIHSQRVLLALTQKGASREDSYRLVQRNAMRVWHGEGDFRTFLLNDA
DVRKYLSEDDINEKFDLGYHFKNVDNIFKRVFGRA
>Mature_435_residues
MISRYSRPEMAAIWSPEARFRIWFEIEAHAADAMADLGIVPKEAAAKIWELGGKAEFDVARIDAIERDVKHDVIAFLTHL
AEFVGPEARFVHQGMTSSDVLDTCLAVQLSRACDLLIADVDALLKVLERRAFEFKLTPTVGRSHGIHAEPTTFGVKLAIA
AAEFQRARARLVAARAEIATCAISGAVGTFAHIDPAVEAHVAQKLGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
TEVRHLQRTEVLEAEEYFSPEQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALARLTGVMDKLVVHTDNMQKNMDKLGGLIHSQRVLLALTQKGASREDSYRLVQRNAMRVWHGEGDFRTFLLNDA
DVRKYLSEDDINEKFDLGYHFKNVDNIFKRVFGRA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=406, Percent_Identity=26.6009852216749, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=26.4935064935065, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1787376, Length=348, Percent_Identity=29.3103448275862, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=399, Percent_Identity=26.3157894736842, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI32564234, Length=363, Percent_Identity=26.7217630853994, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=405, Percent_Identity=27.9012345679012, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24647570, Length=387, Percent_Identity=25.8397932816537, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48448; Mature: 48448

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRYSRPEMAAIWSPEARFRIWFEIEAHAADAMADLGIVPKEAAAKIWELGGKAEFDVA
CCCCCCCCCEEEEECCCCCEEEEEEEECHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHH
RIDAIERDVKHDVIAFLTHLAEFVGPEARFVHQGMTSSDVLDTCLAVQLSRACDLLIADV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DALLKVLERRAFEFKLTPTVGRSHGIHAEPTTFGVKLAIAAAEFQRARARLVAARAEIAT
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFAHIDPAVEAHVAQKLGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
TEVRHLQRTEVLEAEEYFSPEQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
WHERDISHSSVERMIGPDATVTLDFALARLTGVMDKLVVHTDNMQKNMDKLGGLIHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LLALTQKGASREDSYRLVQRNAMRVWHGEGDFRTFLLNDADVRKYLSEDDINEKFDLGYH
HHHHHHCCCCCCHHHHHHHHCCCEEEECCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCC
FKNVDNIFKRVFGRA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MISRYSRPEMAAIWSPEARFRIWFEIEAHAADAMADLGIVPKEAAAKIWELGGKAEFDVA
CCCCCCCCCEEEEECCCCCEEEEEEEECHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHH
RIDAIERDVKHDVIAFLTHLAEFVGPEARFVHQGMTSSDVLDTCLAVQLSRACDLLIADV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DALLKVLERRAFEFKLTPTVGRSHGIHAEPTTFGVKLAIAAAEFQRARARLVAARAEIAT
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFAHIDPAVEAHVAQKLGLTVETVSTQVIPRDRHAMFFAVLGVVASSVERLA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
TEVRHLQRTEVLEAEEYFSPEQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
WHERDISHSSVERMIGPDATVTLDFALARLTGVMDKLVVHTDNMQKNMDKLGGLIHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LLALTQKGASREDSYRLVQRNAMRVWHGEGDFRTFLLNDADVRKYLSEDDINEKFDLGYH
HHHHHHCCCCCCHHHHHHHHCCCEEEECCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCC
FKNVDNIFKRVFGRA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]