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The map label for this gene is rfaF [H]

Identifier: 154247694

GI number: 154247694

Start: 4177402

End: 4178457

Strand: Direct

Name: rfaF [H]

Synonym: Xaut_3770

Alternate gene names: 154247694

Gene position: 4177402-4178457 (Clockwise)

Preceding gene: 154247693

Following gene: 154247695

Centisome position: 78.69

GC content: 72.82

Gene sequence:

>1056_bases
ATGACGCGGGCGTTCCACCCCGCGAGCGTTCGTATGCGTGCCCTTGGTTCCGTTTCGCCCCTGCTGGTTGTCGGCCCCTC
CTGGGTCGGCGACATGGTCATGGCGGCGAGCCTCGTCGAGGTGCTGCGCCTGCGTGAGCCCGGCCGTCCGATCGATGTGC
TGGCGCCCCCCGCCGCGGTTCCCATTGCCCGCATGATCCCCGGCGTCCGGCGCGCTATTCCCCTCGGCCTCGGCCACGGC
GAGCTTGGCCTCATGGCCCGCTGGCGGGCCGGGCGCGCGCTGCGCAACGAGGGCTACGGCAAGGCCATTATCCTGCCCCG
CGCCTTCAAGGCGGCCATCGTCCCCCTCGCCGCCGGCATTCCCGAGCGCGTGGGCTACAAGGCGGAACTGCGCAGCCCGC
TGCTCACCGACGCCCGGCCCGATCCTGACCGCAAGACCGCCCGCACCCTCGACCGCTTCGTGGCGCTGGGCCTGCCGGTG
GGCTCCACCGCCCGCCCGCCGCGCCCGCGCCTGATGCTGCCAGATGGCGCGGTGGACGTGGCCGCTGCCAAGTTCCCGCT
TGCCGGCGAAGGCCCGGTGATGGTGCTGTGCCCCGGCGCCGAATACGGCCCCGCCAAGCGCTGGCCGGCGTCCAAGTTCG
CGGCCCTCGCCGCGCTGGCTGTCGCCGCGGGCTACCGCATCCGCATCCTCGGCTCGCCCAAGGACCAGGAGGCGGCTGAC
GCCATCGCGGCGGGTGCCGGCGTGCCGGTGGACAATCTGGTGGGCCGCACCAGCCTCGTCGAAGCCGCTGCCATCCTCGC
CGGCGCGGACGTGGTGATCTCCAACGATTCCGGCCTGATGCACGTGGCCGGTGCCTTCGACCGACGGCTGGTGGTGCTCT
ATGGCGCCTCCTCCGAGAAGATGACGCCCCCCGCCTCGCCGTTTGCCCGGGTGATGGCGCGTGATCTGCCATGCCGTCCC
TGCCACAAGCGGGAATGCCCGCTCGGCACCCTCGCCTGCCTCGAAGGCATCGCCCCCGAAGAGGTTCTCGCCGCCGCCGA
GCAGATGCGCCGATGA

Upstream 100 bases:

>100_bases
GTGAGATGACGTTCGGTCCGGCCCTGCCGGACCGTCCGATCCCGGCCGCTTGCCCGCCGCGTGCGTTGCTTGACGCTTGA
CGCGGCGGGTCCGCCTTGCA

Downstream 100 bases:

>100_bases
AGGTGCTGCTGGTGAAGCTGTCGTCGCTGGGTGACGTGGTGCACACGTTCCCGGCGCTGACGGACGCCATGGCCGCGCAC
CCCGGTCTTCAGCTCGACTG

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTRAFHPASVRMRALGSVSPLLVVGPSWVGDMVMAASLVEVLRLREPGRPIDVLAPPAAVPIARMIPGVRRAIPLGLGHG
ELGLMARWRAGRALRNEGYGKAIILPRAFKAAIVPLAAGIPERVGYKAELRSPLLTDARPDPDRKTARTLDRFVALGLPV
GSTARPPRPRLMLPDGAVDVAAAKFPLAGEGPVMVLCPGAEYGPAKRWPASKFAALAALAVAAGYRIRILGSPKDQEAAD
AIAAGAGVPVDNLVGRTSLVEAAAILAGADVVISNDSGLMHVAGAFDRRLVVLYGASSEKMTPPASPFARVMARDLPCRP
CHKRECPLGTLACLEGIAPEEVLAAAEQMRR

Sequences:

>Translated_351_residues
MTRAFHPASVRMRALGSVSPLLVVGPSWVGDMVMAASLVEVLRLREPGRPIDVLAPPAAVPIARMIPGVRRAIPLGLGHG
ELGLMARWRAGRALRNEGYGKAIILPRAFKAAIVPLAAGIPERVGYKAELRSPLLTDARPDPDRKTARTLDRFVALGLPV
GSTARPPRPRLMLPDGAVDVAAAKFPLAGEGPVMVLCPGAEYGPAKRWPASKFAALAALAVAAGYRIRILGSPKDQEAAD
AIAAGAGVPVDNLVGRTSLVEAAAILAGADVVISNDSGLMHVAGAFDRRLVVLYGASSEKMTPPASPFARVMARDLPCRP
CHKRECPLGTLACLEGIAPEEVLAAAEQMRR
>Mature_350_residues
TRAFHPASVRMRALGSVSPLLVVGPSWVGDMVMAASLVEVLRLREPGRPIDVLAPPAAVPIARMIPGVRRAIPLGLGHGE
LGLMARWRAGRALRNEGYGKAIILPRAFKAAIVPLAAGIPERVGYKAELRSPLLTDARPDPDRKTARTLDRFVALGLPVG
STARPPRPRLMLPDGAVDVAAAKFPLAGEGPVMVLCPGAEYGPAKRWPASKFAALAALAVAAGYRIRILGSPKDQEAADA
IAAGAGVPVDNLVGRTSLVEAAAILAGADVVISNDSGLMHVAGAFDRRLVVLYGASSEKMTPPASPFARVMARDLPCRPC
HKRECPLGTLACLEGIAPEEVLAAAEQMRR

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=334, Percent_Identity=40.4191616766467, Blast_Score=237, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 36834; Mature: 36703

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAFHPASVRMRALGSVSPLLVVGPSWVGDMVMAASLVEVLRLREPGRPIDVLAPPAAV
CCCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
PIARMIPGVRRAIPLGLGHGELGLMARWRAGRALRNEGYGKAIILPRAFKAAIVPLAAGI
CHHHHCCHHHHHHCCCCCCCCCCHHHHHHHCHHHHCCCCCEEEEECHHHHHHHHHHHCCC
PERVGYKAELRSPLLTDARPDPDRKTARTLDRFVALGLPVGSTARPPRPRLMLPDGAVDV
HHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCH
AAAKFPLAGEGPVMVLCPGAEYGPAKRWPASKFAALAALAVAAGYRIRILGSPKDQEAAD
HHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
AIAAGAGVPVDNLVGRTSLVEAAAILAGADVVISNDSGLMHVAGAFDRRLVVLYGASSEK
HHHHCCCCCHHHHHCHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCEEEEEECCCCCC
MTPPASPFARVMARDLPCRPCHKRECPLGTLACLEGIAPEEVLAAAEQMRR
CCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TRAFHPASVRMRALGSVSPLLVVGPSWVGDMVMAASLVEVLRLREPGRPIDVLAPPAAV
CCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
PIARMIPGVRRAIPLGLGHGELGLMARWRAGRALRNEGYGKAIILPRAFKAAIVPLAAGI
CHHHHCCHHHHHHCCCCCCCCCCHHHHHHHCHHHHCCCCCEEEEECHHHHHHHHHHHCCC
PERVGYKAELRSPLLTDARPDPDRKTARTLDRFVALGLPVGSTARPPRPRLMLPDGAVDV
HHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCH
AAAKFPLAGEGPVMVLCPGAEYGPAKRWPASKFAALAALAVAAGYRIRILGSPKDQEAAD
HHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
AIAAGAGVPVDNLVGRTSLVEAAAILAGADVVISNDSGLMHVAGAFDRRLVVLYGASSEK
HHHHCCCCCHHHHHCHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCEEEEEECCCCCC
MTPPASPFARVMARDLPCRPCHKRECPLGTLACLEGIAPEEVLAAAEQMRR
CCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]