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The map label for this gene is yiaO [H]

Identifier: 154246743

GI number: 154246743

Start: 3123371

End: 3124390

Strand: Reverse

Name: yiaO [H]

Synonym: Xaut_2804

Alternate gene names: 154246743

Gene position: 3124390-3123371 (Counterclockwise)

Preceding gene: 154246750

Following gene: 154246738

Centisome position: 58.85

GC content: 65.0

Gene sequence:

>1020_bases
ATGATGTTGACCCGTCGCACCCTTTTCAAGGCCGGCGCGGCCGGAGCCCTTGCGGGCGGCGTGTTCGCCCCCTTCGTCGC
GCGGGCGCAGAGCGCGGAATTCTCCTACAAATACGCCAACAACCTGCCGGTCACGCACCCCATGAACGTGCGCGCGGGGG
AGATGGTGGACAAGATCAAGGCCGAGACCCAAGGCCGCGTGGAGATCAAGGTGTTCCCCTCCAGCCAGCTCGGCGGCGAC
ACCGACATGCTGAGCCAGCTGCGCTCGGGCGGCATCGAGTTCTTCACCCTCTCCCCGCTCATCCTCTCCACCCTGATCCC
GAACGCCTCGGTGAGCGGCATCGGCTTCGCCTTCCCGGATTACGCCACCGTGTGGAAGGCCATGGATGGCGGGCTCGGCG
CCTATGTGCGCGGCCAGATCTCCAAGGCCGGCATCATCGCCATGGACAATATCTGGGACAACGGCTTCCGCCAGATCACC
ACCTCCACGAAGCCGATCGAGACCGCCGCCGACCTCAAGGGCCTGAAGATCCGGGTGCCGGTGTCGCCGCTGTGGACCTC
CATGTTCCGGGCCTTCGACAGTGCCCCCGCGAGCCTCAACTTCGCCGAGGTCTATACCGCGCTCCAGACCCGCACCGTGG
ATGCGCAGGAGAACCCGCTGGCCATCATCGCCACGGCGAAGCTCTATGAGGTGCAGAAATACTGCTCCATCACCAACCAC
ATGTGGGACGGCTTCTGGTTCCTCGCCAACCGCCGCGCCTTCGAACGCCTGCCGGACGATCTGCGCGCTATCGTGGCGAA
GAACATCAACGCCGCCGGCCTCAAGGAGCGCGAGGACGTGCTGGTGCTCAACGGCAACCTGCGCAAGGAGCTGAGCGAGA
AGGGCCTCGCCTTCAACGAGCCCAAGGTGGACAGTTTCCGCGAGCAGCTGCGCAAGGCCGGCTTCTATTCCGAGTGGAAG
GGCAAGTACGGCGACGAGGCCTGGTCCATCCTGGAGAGCTACACCGGCAAGCTGTCCTGA

Upstream 100 bases:

>100_bases
AATCCCACAAGCGGGCCAGCATGGGCCGCAGGGATACGAGGTCCATATACTGGACCCATAGGAAACCGGTCCGAATGGAC
CGCCAGGAGGAGCTGCCAGC

Downstream 100 bases:

>100_bases
GGGACCGTTTGAGAACACGGTCGGACCGGCGCTGTGTCACCGTCATGGCCGGGCTTGGAAGAGCTTTCGCAAGCTGTCCC
AGGCGGCTCGGCGTGGATGC

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MMLTRRTLFKAGAAGALAGGVFAPFVARAQSAEFSYKYANNLPVTHPMNVRAGEMVDKIKAETQGRVEIKVFPSSQLGGD
TDMLSQLRSGGIEFFTLSPLILSTLIPNASVSGIGFAFPDYATVWKAMDGGLGAYVRGQISKAGIIAMDNIWDNGFRQIT
TSTKPIETAADLKGLKIRVPVSPLWTSMFRAFDSAPASLNFAEVYTALQTRTVDAQENPLAIIATAKLYEVQKYCSITNH
MWDGFWFLANRRAFERLPDDLRAIVAKNINAAGLKEREDVLVLNGNLRKELSEKGLAFNEPKVDSFREQLRKAGFYSEWK
GKYGDEAWSILESYTGKLS

Sequences:

>Translated_339_residues
MMLTRRTLFKAGAAGALAGGVFAPFVARAQSAEFSYKYANNLPVTHPMNVRAGEMVDKIKAETQGRVEIKVFPSSQLGGD
TDMLSQLRSGGIEFFTLSPLILSTLIPNASVSGIGFAFPDYATVWKAMDGGLGAYVRGQISKAGIIAMDNIWDNGFRQIT
TSTKPIETAADLKGLKIRVPVSPLWTSMFRAFDSAPASLNFAEVYTALQTRTVDAQENPLAIIATAKLYEVQKYCSITNH
MWDGFWFLANRRAFERLPDDLRAIVAKNINAAGLKEREDVLVLNGNLRKELSEKGLAFNEPKVDSFREQLRKAGFYSEWK
GKYGDEAWSILESYTGKLS
>Mature_339_residues
MMLTRRTLFKAGAAGALAGGVFAPFVARAQSAEFSYKYANNLPVTHPMNVRAGEMVDKIKAETQGRVEIKVFPSSQLGGD
TDMLSQLRSGGIEFFTLSPLILSTLIPNASVSGIGFAFPDYATVWKAMDGGLGAYVRGQISKAGIIAMDNIWDNGFRQIT
TSTKPIETAADLKGLKIRVPVSPLWTSMFRAFDSAPASLNFAEVYTALQTRTVDAQENPLAIIATAKLYEVQKYCSITNH
MWDGFWFLANRRAFERLPDDLRAIVAKNINAAGLKEREDVLVLNGNLRKELSEKGLAFNEPKVDSFREQLRKAGFYSEWK
GKYGDEAWSILESYTGKLS

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=273, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37392; Mature: 37392

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLTRRTLFKAGAAGALAGGVFAPFVARAQSAEFSYKYANNLPVTHPMNVRAGEMVDKIK
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHH
AETQGRVEIKVFPSSQLGGDTDMLSQLRSGGIEFFTLSPLILSTLIPNASVSGIGFAFPD
HCCCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCH
YATVWKAMDGGLGAYVRGQISKAGIIAMDNIWDNGFRQITTSTKPIETAADLKGLKIRVP
HHHHHHHHCCCCCCEEECCCCCCCEEEECCCCCCCHHEEECCCCCCCHHHCCCCCEEEEC
VSPLWTSMFRAFDSAPASLNFAEVYTALQTRTVDAQENPLAIIATAKLYEVQKYCSITNH
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
MWDGFWFLANRRAFERLPDDLRAIVAKNINAAGLKEREDVLVLNGNLRKELSEKGLAFNE
HCCCEEEECCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCC
PKVDSFREQLRKAGFYSEWKGKYGDEAWSILESYTGKLS
CCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMLTRRTLFKAGAAGALAGGVFAPFVARAQSAEFSYKYANNLPVTHPMNVRAGEMVDKIK
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHH
AETQGRVEIKVFPSSQLGGDTDMLSQLRSGGIEFFTLSPLILSTLIPNASVSGIGFAFPD
HCCCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCH
YATVWKAMDGGLGAYVRGQISKAGIIAMDNIWDNGFRQITTSTKPIETAADLKGLKIRVP
HHHHHHHHCCCCCCEEECCCCCCCEEEECCCCCCCHHEEECCCCCCCHHHCCCCCEEEEC
VSPLWTSMFRAFDSAPASLNFAEVYTALQTRTVDAQENPLAIIATAKLYEVQKYCSITNH
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
MWDGFWFLANRRAFERLPDDLRAIVAKNINAAGLKEREDVLVLNGNLRKELSEKGLAFNE
HCCCEEEECCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCC
PKVDSFREQLRKAGFYSEWKGKYGDEAWSILESYTGKLS
CCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]