Click here to switch to the map view.

The map label for this gene is nasA [H]

Identifier: 154246438

GI number: 154246438

Start: 2788562

End: 2791240

Strand: Reverse

Name: nasA [H]

Synonym: Xaut_2497

Alternate gene names: 154246438

Gene position: 2791240-2788562 (Counterclockwise)

Preceding gene: 154246439

Following gene: 154246435

Centisome position: 52.58

GC content: 72.42

Gene sequence:

>2679_bases
ATGAACGCGCCTCTCGCCTCGCCCCCCTCGGTGAAGACCACCTGCCCCTATTGCGGCGTCGGCTGCGGGGTGAAGGCGAC
GCCGGACGGCCTGGGCGGCGCGCTGGTCGAAGGCGATGCCGCCCACCCGGCCAATCTCGGCCGCCTGTGCTCCAAGGGCG
CCGCGCTGGGGGAGACGGTGGGGCTCGATACCCGCCTGCTCCACCCCCTGGTGCGCGGGACTGACGGGGCGCTCGCCCGC
GCCTCGTGGGACACCGCCCTCGACCGCATCGCGGACGGGCTGAAGACGGTGCGCGACGCCCACGGGCCGGGAGCCATCGC
CTTCTATCTCTCCGGCCAGTTGCTGACCGAGGATTATTACGCCGCCAACAAGCTGGCGAAGGGCTTCCTCGGCACCGCGC
ACGTGGACACCAATTCGCGCCTGTGCATGTCCTCCTCCGTGGCCGGGCACAAGCGCGGCTTCGGTTCGGACACGGTGCCG
GGCTCCTATGAGGATTTGGAGGCGGCGGATCTCATCGTTCTGGTGGGCTCCAACACCGCCTGGTGCCATCCGGTGCTGTT
CCGCCGCATGGAGGCGGCGCGCAAGGCGCGGGGCACGAAATTCGTGGTCATCGACCCCCGCCGCACCGAGACGGCGGCGG
AAGCCGACCTGCACCTGCCGCTGGCCCACGGCGCCGACGCGCTGCTGTTTTCCGGCCTGCTCGCCCATCTGGCCCAGAGC
CCGGCCTTCGACGCCGATTACGTGCGCGACCACACCACCGGCTTCGACGCCGCGCTCGCCCACGCCCGCGCCATGGCCCC
CGATGCCGAGGCCACCGCCCGCGCCTGCGGCCTTGCGGTGGAAGACGTGGCGCGCTTCTTCGCCCTGTTCACGGACACCG
CGCGCACCGTGACCTGCTATTCGCAGGGGGTGAACCAGTCGGTGGCGGGCACCGACAAGGTCAATGCCATCCTCAACTGC
CATTTCGCCACCGGCCGCATGGGCCGCGAGGGCATGGGGCCGTTCTCCCTCACCGGCCAGCCCAATGCCATGGGCGGGCG
CGAGGTGGGCGGGCTCGCCAACCAGCTGGCCGCCCACATGGGCTTTTCCCCGGAGGAGGTGGACCGGGTGCGCCGCTTCT
GGGATGCGCCCCATATGGCCACCCGCGAGGGCCACAAGGCGGTGGACCTGTTCGCCGCCATCGGCCGTGGCGAGATCAAG
GCGCTGTGGGTCATGGGCACCAACCCGGCCGTGAGCCTGCCCGAGGCGGACGGCGTGCGCGCGGCGCTGAAGGGCCTCGA
CCTGTTCGTGCTGTCCGAGAACGTGGTGCACAACGACACCGCCGCCTGCCATCCCCATGTGATGCTGCCCGCCGCCGCCT
GGGGCGAGAAGGACGGCACCGTCACCAATTCCGAGCGGCGCATTTCCCGCCAGCGGCCCTTCCTCGCCCTGCCCGGTGCG
GTGAAGCCCAATTGGTGGGCGCTGGCGCAGGTGGCGAGCCGCCTTGGCTTCGGCGCGGCCTTCGCGTGGCGCGGGCCGGC
GGATATCTTCCGCGAGCATGCGGCGCTCTCCGGCTTCGAGAATGCCGGCAGCCGCGATTTCGACATCTCCGCCTTCGCCG
ACCTGTCGGACACGGCCTACCAGGATTTCACACCGGTACAGTGGCCGGCCCCGCGCGGGCGGCCGCAGGGCACACCACGG
CTGTTCGCGGACGGCGGCTATTTCACCGCCGACCGGCGCGCCCGCTTCGTGTCGCCTGCGCCGGTGGCAGCGGCGCGGGC
GAGCGAGGCCTACCCGTTCCTGCTCAACACCGGCCGCATCCGCGACCAGTGGCACACCATGACCCGCACCGGCCTGTCGC
CGCGCCTCGGCTCGCACATCCTGGCGCCGTTCGTGGCCGTGCATCCGGACGATGCGGCGCCCCTCGGCCTTGCCGACGGC
GGGCTGGCGCGAGTGTCGAGCGCCCATGGCGCGGCGGTGCTGGAGGTGAAGATCGATGCCGGGCAAAAGCGCGGCACCCT
GTTCGCGCCCATCCACTGGAGCGGGGCGACCTCCTCCCACGGGCGGGTCGGGCCGCTGGTGCACGCGGTCACCGACCCCT
TCTCCGGCCAGCCCGATTCCAAGGCGACACCGGCCACCCTCGCACCCTTTACCGCGCCGTTCGAAGGCTTCGTGCTGTCG
CGGCGCCCACTTGCGATGCCGCAGGGCCTGTGGTGGGCGCGGGCGGCGCTGGAGGGCGGCTTCGGCTGGCGCACCGCGGG
CAGCCTCGGCCCGGACGAGTGGGCGCAGTGGGTGAAGGCGGAAGGCCCGGCGGACGTGGCGGAATTGGTAGACGGCCCCT
CCGGCCTCTACAGGGCTGCCGCCTTCGACGCCGACGGGCGGCTGGATTTCGCGGTGTTCGAGGGCACGGCCGGCCGGCGC
GTGGCCTGGGACGCGCTGAAGGGCCTGCTTAAGGAAGACACCGTCTCCACCGCCGACCGGCGCACCCTGCTGGCGGGCCG
GCGGGCGGGAGCTGCAGCCGCATGCGGTCCGCTGGTCTGCGCGTGCTTCGGCGTGCCGCGCGACGCCATCGTGGGCGCCA
TCCGCGCCGGCGCGCACGATGCGGCCATGGTGGGGGAAAAGCTCAAGGCCGGCACGAACTGCGGCTCATGCCTGCCCGAG
ATCCGCCGCCTCCTCGCGGAGACGACGGTACCGGCCTGA

Upstream 100 bases:

>100_bases
GGAGGCCGCGGCCCGCTTCGTGGCCGACCTGAAGACCGCCGGCCGCTACCAGGCCGACGTGTACTGACCCCCGAAACGCC
TCAGGCAGGATCGGCCCCCC

Downstream 100 bases:

>100_bases
GGGGCGGGTGGTCCCGCCCCTTTCGAGCGTGTGCCGCTCGACTGGCTCACTAGAGCGTTTTCTTCACGCGAACCGGAAAT
CCACTTCGCTCGAAAACGCT

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 892; Mature: 892

Protein sequence:

>892_residues
MNAPLASPPSVKTTCPYCGVGCGVKATPDGLGGALVEGDAAHPANLGRLCSKGAALGETVGLDTRLLHPLVRGTDGALAR
ASWDTALDRIADGLKTVRDAHGPGAIAFYLSGQLLTEDYYAANKLAKGFLGTAHVDTNSRLCMSSSVAGHKRGFGSDTVP
GSYEDLEAADLIVLVGSNTAWCHPVLFRRMEAARKARGTKFVVIDPRRTETAAEADLHLPLAHGADALLFSGLLAHLAQS
PAFDADYVRDHTTGFDAALAHARAMAPDAEATARACGLAVEDVARFFALFTDTARTVTCYSQGVNQSVAGTDKVNAILNC
HFATGRMGREGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFSPEEVDRVRRFWDAPHMATREGHKAVDLFAAIGRGEIK
ALWVMGTNPAVSLPEADGVRAALKGLDLFVLSENVVHNDTAACHPHVMLPAAAWGEKDGTVTNSERRISRQRPFLALPGA
VKPNWWALAQVASRLGFGAAFAWRGPADIFREHAALSGFENAGSRDFDISAFADLSDTAYQDFTPVQWPAPRGRPQGTPR
LFADGGYFTADRRARFVSPAPVAAARASEAYPFLLNTGRIRDQWHTMTRTGLSPRLGSHILAPFVAVHPDDAAPLGLADG
GLARVSSAHGAAVLEVKIDAGQKRGTLFAPIHWSGATSSHGRVGPLVHAVTDPFSGQPDSKATPATLAPFTAPFEGFVLS
RRPLAMPQGLWWARAALEGGFGWRTAGSLGPDEWAQWVKAEGPADVAELVDGPSGLYRAAAFDADGRLDFAVFEGTAGRR
VAWDALKGLLKEDTVSTADRRTLLAGRRAGAAAACGPLVCACFGVPRDAIVGAIRAGAHDAAMVGEKLKAGTNCGSCLPE
IRRLLAETTVPA

Sequences:

>Translated_892_residues
MNAPLASPPSVKTTCPYCGVGCGVKATPDGLGGALVEGDAAHPANLGRLCSKGAALGETVGLDTRLLHPLVRGTDGALAR
ASWDTALDRIADGLKTVRDAHGPGAIAFYLSGQLLTEDYYAANKLAKGFLGTAHVDTNSRLCMSSSVAGHKRGFGSDTVP
GSYEDLEAADLIVLVGSNTAWCHPVLFRRMEAARKARGTKFVVIDPRRTETAAEADLHLPLAHGADALLFSGLLAHLAQS
PAFDADYVRDHTTGFDAALAHARAMAPDAEATARACGLAVEDVARFFALFTDTARTVTCYSQGVNQSVAGTDKVNAILNC
HFATGRMGREGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFSPEEVDRVRRFWDAPHMATREGHKAVDLFAAIGRGEIK
ALWVMGTNPAVSLPEADGVRAALKGLDLFVLSENVVHNDTAACHPHVMLPAAAWGEKDGTVTNSERRISRQRPFLALPGA
VKPNWWALAQVASRLGFGAAFAWRGPADIFREHAALSGFENAGSRDFDISAFADLSDTAYQDFTPVQWPAPRGRPQGTPR
LFADGGYFTADRRARFVSPAPVAAARASEAYPFLLNTGRIRDQWHTMTRTGLSPRLGSHILAPFVAVHPDDAAPLGLADG
GLARVSSAHGAAVLEVKIDAGQKRGTLFAPIHWSGATSSHGRVGPLVHAVTDPFSGQPDSKATPATLAPFTAPFEGFVLS
RRPLAMPQGLWWARAALEGGFGWRTAGSLGPDEWAQWVKAEGPADVAELVDGPSGLYRAAAFDADGRLDFAVFEGTAGRR
VAWDALKGLLKEDTVSTADRRTLLAGRRAGAAAACGPLVCACFGVPRDAIVGAIRAGAHDAAMVGEKLKAGTNCGSCLPE
IRRLLAETTVPA
>Mature_892_residues
MNAPLASPPSVKTTCPYCGVGCGVKATPDGLGGALVEGDAAHPANLGRLCSKGAALGETVGLDTRLLHPLVRGTDGALAR
ASWDTALDRIADGLKTVRDAHGPGAIAFYLSGQLLTEDYYAANKLAKGFLGTAHVDTNSRLCMSSSVAGHKRGFGSDTVP
GSYEDLEAADLIVLVGSNTAWCHPVLFRRMEAARKARGTKFVVIDPRRTETAAEADLHLPLAHGADALLFSGLLAHLAQS
PAFDADYVRDHTTGFDAALAHARAMAPDAEATARACGLAVEDVARFFALFTDTARTVTCYSQGVNQSVAGTDKVNAILNC
HFATGRMGREGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFSPEEVDRVRRFWDAPHMATREGHKAVDLFAAIGRGEIK
ALWVMGTNPAVSLPEADGVRAALKGLDLFVLSENVVHNDTAACHPHVMLPAAAWGEKDGTVTNSERRISRQRPFLALPGA
VKPNWWALAQVASRLGFGAAFAWRGPADIFREHAALSGFENAGSRDFDISAFADLSDTAYQDFTPVQWPAPRGRPQGTPR
LFADGGYFTADRRARFVSPAPVAAARASEAYPFLLNTGRIRDQWHTMTRTGLSPRLGSHILAPFVAVHPDDAAPLGLADG
GLARVSSAHGAAVLEVKIDAGQKRGTLFAPIHWSGATSSHGRVGPLVHAVTDPFSGQPDSKATPATLAPFTAPFEGFVLS
RRPLAMPQGLWWARAALEGGFGWRTAGSLGPDEWAQWVKAEGPADVAELVDGPSGLYRAAAFDADGRLDFAVFEGTAGRR
VAWDALKGLLKEDTVSTADRRTLLAGRRAGAAAACGPLVCACFGVPRDAIVGAIRAGAHDAAMVGEKLKAGTNCGSCLPE
IRRLLAETTVPA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=793, Percent_Identity=26.860025220681, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI3868721, Length=713, Percent_Identity=24.9649368863955, Blast_Score=236, Evalue=7e-63,
Organism=Escherichia coli, GI3868719, Length=599, Percent_Identity=24.7078464106845, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI3868720, Length=594, Percent_Identity=25.5892255892256, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1787778, Length=384, Percent_Identity=25.5208333333333, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1787870, Length=717, Percent_Identity=21.1994421199442, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93857; Mature: 93857

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPLASPPSVKTTCPYCGVGCGVKATPDGLGGALVEGDAAHPANLGRLCSKGAALGETV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHCCCCCCHHC
GLDTRLLHPLVRGTDGALARASWDTALDRIADGLKTVRDAHGPGAIAFYLSGQLLTEDYY
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEHHHHH
AANKLAKGFLGTAHVDTNSRLCMSSSVAGHKRGFGSDTVPGSYEDLEAADLIVLVGSNTA
HHHHHHHHCCCEEEECCCCCEEECCCCCCHHCCCCCCCCCCCCCCCCCCCEEEEECCCCC
WCHPVLFRRMEAARKARGTKFVVIDPRRTETAAEADLHLPLAHGADALLFSGLLAHLAQS
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCEEEEHHCCCHHHHHHHHHHHHHCC
PAFDADYVRDHTTGFDAALAHARAMAPDAEATARACGLAVEDVARFFALFTDTARTVTCY
CCCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEH
SQGVNQSVAGTDKVNAILNCHFATGRMGREGMGPFSLTGQPNAMGGREVGGLANQLAAHM
HCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHC
GFSPEEVDRVRRFWDAPHMATREGHKAVDLFAAIGRGEIKALWVMGTNPAVSLPEADGVR
CCCHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHH
AALKGLDLFVLSENVVHNDTAACHPHVMLPAAAWGEKDGTVTNSERRISRQRPFLALPGA
HHHHCCEEEEEECCEECCCCCCCCCCEEECHHHCCCCCCCCCCHHHHHHHCCCCEECCCC
VKPNWWALAQVASRLGFGAAFAWRGPADIFREHAALSGFENAGSRDFDISAFADLSDTAY
CCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCC
QDFTPVQWPAPRGRPQGTPRLFADGGYFTADRRARFVSPAPVAAARASEAYPFLLNTGRI
CCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCEECCCCCHHHHHCCCCCCEEEECCCC
RDQWHTMTRTGLSPRLGSHILAPFVAVHPDDAAPLGLADGGLARVSSAHGAAVLEVKIDA
HHHHHHHHHCCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECC
GQKRGTLFAPIHWSGATSSHGRVGPLVHAVTDPFSGQPDSKATPATLAPFTAPFEGFVLS
CCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCEEEC
RRPLAMPQGLWWARAALEGGFGWRTAGSLGPDEWAQWVKAEGPADVAELVDGPSGLYRAA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCHHHEEE
AFDADGRLDFAVFEGTAGRRVAWDALKGLLKEDTVSTADRRTLLAGRRAGAAAACGPLVC
EECCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHH
ACFGVPRDAIVGAIRAGAHDAAMVGEKLKAGTNCGSCLPEIRRLLAETTVPA
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNAPLASPPSVKTTCPYCGVGCGVKATPDGLGGALVEGDAAHPANLGRLCSKGAALGETV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHCCCCCCHHC
GLDTRLLHPLVRGTDGALARASWDTALDRIADGLKTVRDAHGPGAIAFYLSGQLLTEDYY
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEHHHHH
AANKLAKGFLGTAHVDTNSRLCMSSSVAGHKRGFGSDTVPGSYEDLEAADLIVLVGSNTA
HHHHHHHHCCCEEEECCCCCEEECCCCCCHHCCCCCCCCCCCCCCCCCCCEEEEECCCCC
WCHPVLFRRMEAARKARGTKFVVIDPRRTETAAEADLHLPLAHGADALLFSGLLAHLAQS
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCEEEEHHCCCHHHHHHHHHHHHHCC
PAFDADYVRDHTTGFDAALAHARAMAPDAEATARACGLAVEDVARFFALFTDTARTVTCY
CCCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEH
SQGVNQSVAGTDKVNAILNCHFATGRMGREGMGPFSLTGQPNAMGGREVGGLANQLAAHM
HCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHC
GFSPEEVDRVRRFWDAPHMATREGHKAVDLFAAIGRGEIKALWVMGTNPAVSLPEADGVR
CCCHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHH
AALKGLDLFVLSENVVHNDTAACHPHVMLPAAAWGEKDGTVTNSERRISRQRPFLALPGA
HHHHCCEEEEEECCEECCCCCCCCCCEEECHHHCCCCCCCCCCHHHHHHHCCCCEECCCC
VKPNWWALAQVASRLGFGAAFAWRGPADIFREHAALSGFENAGSRDFDISAFADLSDTAY
CCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCC
QDFTPVQWPAPRGRPQGTPRLFADGGYFTADRRARFVSPAPVAAARASEAYPFLLNTGRI
CCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCEECCCCCHHHHHCCCCCCEEEECCCC
RDQWHTMTRTGLSPRLGSHILAPFVAVHPDDAAPLGLADGGLARVSSAHGAAVLEVKIDA
HHHHHHHHHCCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECC
GQKRGTLFAPIHWSGATSSHGRVGPLVHAVTDPFSGQPDSKATPATLAPFTAPFEGFVLS
CCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCEEEC
RRPLAMPQGLWWARAALEGGFGWRTAGSLGPDEWAQWVKAEGPADVAELVDGPSGLYRAA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCHHHEEE
AFDADGRLDFAVFEGTAGRRVAWDALKGLLKEDTVSTADRRTLLAGRRAGAAAACGPLVC
EECCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHH
ACFGVPRDAIVGAIRAGAHDAAMVGEKLKAGTNCGSCLPEIRRLLAETTVPA
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]