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The map label for this gene is menB [C]

Identifier: 154246426

GI number: 154246426

Start: 2777656

End: 2778438

Strand: Reverse

Name: menB [C]

Synonym: Xaut_2485

Alternate gene names: 154246426

Gene position: 2778438-2777656 (Counterclockwise)

Preceding gene: 154246427

Following gene: 154246425

Centisome position: 52.34

GC content: 70.75

Gene sequence:

>783_bases
GTGGCTGGCAGCATCGCCATTTCCGTCGAGGGCGGCATCGCCCGCCTTGTGCTGACCAATCCGGAACGGCGCAACGCCAT
TTCCAGCACCATGTGGCGGGGCCTGATCGCTTTCGCGCAGGACGCCGCCCATCGCAGCGATATCCGCGTTGCGGTGCTGC
GCGGCGAGGGCGACCTCGCCTTTTCCGGTGGCGCCGACATTTCGGATTTCGACACCGCCCGCTCCGATGCCTCGGGCGCG
CAGAGCTATGACGACCTGGTGGAACAGGCCTGCTCGGCCATCGAGAACCTGGCCTGTCCCACCATCTCGCTGGTGAAAGG
CGCGTGCGTGGGCGCCGGCGCGGCGCTGGCGGCGAGCTGCGACATGCGGATCGCCGCGGACGACGCCTTCTTCGCCATTC
CCGCCGCCCGCCTCGGCCTCGGATACGATCCGCGAGGCATGGCGCGGGCGCTGCGGGTCTTCGGCAGCCAGGGCGCGCGG
CACCTGTTCTTCACTGCCGAGCGGCTGACGGCGGCGCGGGCACATGCCATCGGCGCCGTGGATGTGCTCATCCCCGCCGC
CGAGGCGGAAGCCACCACTGAGCGCACGCTCCAGCGCATCGCCGAGAATGCGCCACTGACTCTGAAGGCCGCCAAGCTCG
CCATCCGCGCCGCCGAGACCGGCTCGCAGGCGCTGCTGGGCGAAGCGCGCCGCGCGACCGGTGCGGCCAATGCCAGCGCC
GATTATCGCGAAGGCCGCCTTGCCTTCGCGGAGAAACGCGCGCCGCGCTTCTCCGGAAGCTGA

Upstream 100 bases:

>100_bases
CGGGACAGGGCCGGCCGGGCCTCTGTCGCCGGCCAAAAGCCGGGCAATCCAAGACAGGTTCGAGCACGACCGCTTCGAGA
AGGCCTGGGGGGAGGGACGC

Downstream 100 bases:

>100_bases
GGGCGGCCGCCCGGATGCGGGCGGCGGACTTCCTGGCAGGACCTTGAGGCGCGTGCCTTCCGACCAGGCCCTTCGAGCAC
CGCCAGTCCGGCTATTTGCC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAGSIAISVEGGIARLVLTNPERRNAISSTMWRGLIAFAQDAAHRSDIRVAVLRGEGDLAFSGGADISDFDTARSDASGA
QSYDDLVEQACSAIENLACPTISLVKGACVGAGAALAASCDMRIAADDAFFAIPAARLGLGYDPRGMARALRVFGSQGAR
HLFFTAERLTAARAHAIGAVDVLIPAAEAEATTERTLQRIAENAPLTLKAAKLAIRAAETGSQALLGEARRATGAANASA
DYREGRLAFAEKRAPRFSGS

Sequences:

>Translated_260_residues
MAGSIAISVEGGIARLVLTNPERRNAISSTMWRGLIAFAQDAAHRSDIRVAVLRGEGDLAFSGGADISDFDTARSDASGA
QSYDDLVEQACSAIENLACPTISLVKGACVGAGAALAASCDMRIAADDAFFAIPAARLGLGYDPRGMARALRVFGSQGAR
HLFFTAERLTAARAHAIGAVDVLIPAAEAEATTERTLQRIAENAPLTLKAAKLAIRAAETGSQALLGEARRATGAANASA
DYREGRLAFAEKRAPRFSGS
>Mature_259_residues
AGSIAISVEGGIARLVLTNPERRNAISSTMWRGLIAFAQDAAHRSDIRVAVLRGEGDLAFSGGADISDFDTARSDASGAQ
SYDDLVEQACSAIENLACPTISLVKGACVGAGAALAASCDMRIAADDAFFAIPAARLGLGYDPRGMARALRVFGSQGARH
LFFTAERLTAARAHAIGAVDVLIPAAEAEATTERTLQRIAENAPLTLKAAKLAIRAAETGSQALLGEARRATGAANASAD
YREGRLAFAEKRAPRFSGS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=31.5175097276265, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI70995211, Length=219, Percent_Identity=26.9406392694064, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=29.6577946768061, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=27.6264591439689, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=31.2, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI87082183, Length=241, Percent_Identity=32.3651452282158, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=30.5019305019305, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI221142681, Length=275, Percent_Identity=30.5454545454545, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787660, Length=270, Percent_Identity=31.4814814814815, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=27.1653543307087, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=247, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=199, Percent_Identity=30.6532663316583, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=224, Percent_Identity=27.2321428571429, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=23.6363636363636, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=26.5486725663717, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920382, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=29.3893129770992, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=28.6290322580645, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=28.6290322580645, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=24.7104247104247, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26950; Mature: 26818

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSIAISVEGGIARLVLTNPERRNAISSTMWRGLIAFAQDAAHRSDIRVAVLRGEGDLA
CCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
FSGGADISDFDTARSDASGAQSYDDLVEQACSAIENLACPTISLVKGACVGAGAALAASC
ECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCC
DMRIAADDAFFAIPAARLGLGYDPRGMARALRVFGSQGARHLFFTAERLTAARAHAIGAV
CEEEECCCCEEECCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCHH
DVLIPAAEAEATTERTLQRIAENAPLTLKAAKLAIRAAETGSQALLGEARRATGAANASA
HHEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHEEHHCCCCHHHHHHHHHHCCCCCCCC
DYREGRLAFAEKRAPRFSGS
CCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
AGSIAISVEGGIARLVLTNPERRNAISSTMWRGLIAFAQDAAHRSDIRVAVLRGEGDLA
CCCEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
FSGGADISDFDTARSDASGAQSYDDLVEQACSAIENLACPTISLVKGACVGAGAALAASC
ECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCC
DMRIAADDAFFAIPAARLGLGYDPRGMARALRVFGSQGARHLFFTAERLTAARAHAIGAV
CEEEECCCCEEECCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCHH
DVLIPAAEAEATTERTLQRIAENAPLTLKAAKLAIRAAETGSQALLGEARRATGAANASA
HHEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHEEHHCCCCHHHHHHHHHHCCCCCCCC
DYREGRLAFAEKRAPRFSGS
CCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA