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The map label for this gene is alkK [H]

Identifier: 154246404

GI number: 154246404

Start: 2753429

End: 2755057

Strand: Reverse

Name: alkK [H]

Synonym: Xaut_2463

Alternate gene names: 154246404

Gene position: 2755057-2753429 (Counterclockwise)

Preceding gene: 154246406

Following gene: 154246403

Centisome position: 51.89

GC content: 67.1

Gene sequence:

>1629_bases
ATGGCCGGCGGCATCTATGACCTGCATCTCGACCGCAATCCCGCGAACTTCCAGCCGCTGACGCCGCTCGGCTTCCTGGA
GCGGGCAGCCGGCGTGTTCCCGGACCACACGGCCATCATCCACGGTGCGCTGCGTCGCAACTATCGCGATTTCTATGCGC
GCAGCCGGCGGCTCGCCTCGGCCCTCGCCAAGCTGGGCGTCGGCAAGGGCGACACGGTCGCGGTGATGCTGCCCAACGCG
CCGGCCATGCTGGAGGCCCACTACGGCGTGCCCATGACGGGCGCGGTCCTCAATTCCCTCAATACCCGCCTCGACGCCGC
CATCCTCGCCTTCACCCTCGACCATGGCGAGGCCAAGGTGCTCATCACCGACCGGGAATTCTCCCCCGTCATGAAGGCTG
CTCTGGCCATGGCGACGCGCAAGCCCGTGGTCATCGACTATGACGACCCGGAATTCACCGGCAAGGGCGAGCGCATCGGC
ACCATCGAATACGAGGATTTCCTGGCCACCGGCGACGCCGACTTCGCCTGGAAAACGCCCGACGACGAGTGGGACGCTAT
TGCGCTCAACTACACCTCCGGCACCACCGGCGACCCCAAGGGCGTGGTCTATCACCATCGCGGCGCGCATTTGCTGGCGG
TGGGCAACGTGGTCACCTGCAGCCTCGGCAAGCATCCGGTCTATCTGTGGACGCTGCCCATGTTCCACTGCAACGGCTGG
TGCTTCCCCTGGTCCATCACGCTGGCCGCCGGCACCCATGTGTGCCTGCGCCAGGTACGCGCCAAGCCCATCTTCGACGC
CATCGCCGACTACAAGGTGACGCACATGTGCGGCGCCCCCATCGTCATGTCCACGCTGCTCAACACGCCGGACGCCGACA
AGCGGCCCCTGCCCCACAAGGTGGAGTTCATCACCGCCGCCGCCCCGCCGCCGGAAGCGGTGCTCGCCGCCATGCAGGAG
GCGGGCTTCAACGTGGTGCATGTGTACGGCCTCACCGAGGTCTACGGCCCCTCCGTGGTCAACGAGTGGCACGCCGAGTG
GGACGCCCTGCCCGCGCAGGAGCGCGCCATCAAGAAAGCACGCCAGGGCGTGCGCTACGGCGCGCTCGAAGCCCTCGATG
TGCTGGATCCCGACACCATGGTGCCGGTGCCCGCCGATGGCGAGACGCTGGGCGAGGTGATGTTCCGCGGCAACGTGGTG
ATGAAGGGCTACCTCAAGAACCCCACCGCCACCGACGCTGCGTTCGCCGGCGGCTGGTTCCATTCCGGCGACCTCGGGGT
GAAGCATCCCGACGGCTATATCCAGCTGAAGGACCGCTCCAAGGACATCATCATCTCGGGCGGCGAGAACATCTCCTCCA
TCGAGGTGGAGGACGCCCTCTACAAGCACCCGGCGGTGAGTGCGGCGGCGGTGGTGGCCAAGCCCGACGAGAAGTGGGGC
GAGACCCCGGTCGCCTTCGTCGAGCTGCGCGAGGGCGCGAGCGCGACGGCGGAGGACCTGATCGCCCATTGCCGGACCCA
TCTGGCGGCTTATAAGTGCCCGCGCCACATCGTCTTCGAGGAGATCCCGAAGACGTCGACCGGCAAGATCCAGAAGTTCC
GGCTGCGCGAGATGGCCAAGGAGGTCTGA

Upstream 100 bases:

>100_bases
CTGATCGCCTGCCTCGGCTACGGTCGCGGCCCGAATACAACCGAAATTTCGGCGCGCCGACACCGCCGCGCCCTAAAAGC
CTAGACCAAGGGGGGGACCG

Downstream 100 bases:

>100_bases
GTGGACGTCAAGGACGCGAACGGCGCCATCCTGAAGGATGGCGACAACGTGACCCTCATCAAGGACCTCAAGGTGAAGGG
CACGTCCGTCACCTTGAAGC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MAGGIYDLHLDRNPANFQPLTPLGFLERAAGVFPDHTAIIHGALRRNYRDFYARSRRLASALAKLGVGKGDTVAVMLPNA
PAMLEAHYGVPMTGAVLNSLNTRLDAAILAFTLDHGEAKVLITDREFSPVMKAALAMATRKPVVIDYDDPEFTGKGERIG
TIEYEDFLATGDADFAWKTPDDEWDAIALNYTSGTTGDPKGVVYHHRGAHLLAVGNVVTCSLGKHPVYLWTLPMFHCNGW
CFPWSITLAAGTHVCLRQVRAKPIFDAIADYKVTHMCGAPIVMSTLLNTPDADKRPLPHKVEFITAAAPPPEAVLAAMQE
AGFNVVHVYGLTEVYGPSVVNEWHAEWDALPAQERAIKKARQGVRYGALEALDVLDPDTMVPVPADGETLGEVMFRGNVV
MKGYLKNPTATDAAFAGGWFHSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVAKPDEKWG
ETPVAFVELREGASATAEDLIAHCRTHLAAYKCPRHIVFEEIPKTSTGKIQKFRLREMAKEV

Sequences:

>Translated_542_residues
MAGGIYDLHLDRNPANFQPLTPLGFLERAAGVFPDHTAIIHGALRRNYRDFYARSRRLASALAKLGVGKGDTVAVMLPNA
PAMLEAHYGVPMTGAVLNSLNTRLDAAILAFTLDHGEAKVLITDREFSPVMKAALAMATRKPVVIDYDDPEFTGKGERIG
TIEYEDFLATGDADFAWKTPDDEWDAIALNYTSGTTGDPKGVVYHHRGAHLLAVGNVVTCSLGKHPVYLWTLPMFHCNGW
CFPWSITLAAGTHVCLRQVRAKPIFDAIADYKVTHMCGAPIVMSTLLNTPDADKRPLPHKVEFITAAAPPPEAVLAAMQE
AGFNVVHVYGLTEVYGPSVVNEWHAEWDALPAQERAIKKARQGVRYGALEALDVLDPDTMVPVPADGETLGEVMFRGNVV
MKGYLKNPTATDAAFAGGWFHSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVAKPDEKWG
ETPVAFVELREGASATAEDLIAHCRTHLAAYKCPRHIVFEEIPKTSTGKIQKFRLREMAKEV
>Mature_541_residues
AGGIYDLHLDRNPANFQPLTPLGFLERAAGVFPDHTAIIHGALRRNYRDFYARSRRLASALAKLGVGKGDTVAVMLPNAP
AMLEAHYGVPMTGAVLNSLNTRLDAAILAFTLDHGEAKVLITDREFSPVMKAALAMATRKPVVIDYDDPEFTGKGERIGT
IEYEDFLATGDADFAWKTPDDEWDAIALNYTSGTTGDPKGVVYHHRGAHLLAVGNVVTCSLGKHPVYLWTLPMFHCNGWC
FPWSITLAAGTHVCLRQVRAKPIFDAIADYKVTHMCGAPIVMSTLLNTPDADKRPLPHKVEFITAAAPPPEAVLAAMQEA
GFNVVHVYGLTEVYGPSVVNEWHAEWDALPAQERAIKKARQGVRYGALEALDVLDPDTMVPVPADGETLGEVMFRGNVVM
KGYLKNPTATDAAFAGGWFHSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVAKPDEKWGE
TPVAFVELREGASATAEDLIAHCRTHLAAYKCPRHIVFEEIPKTSTGKIQKFRLREMAKEV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=560, Percent_Identity=28.0357142857143, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI187761345, Length=558, Percent_Identity=27.9569892473118, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI187761343, Length=558, Percent_Identity=27.9569892473118, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI58082049, Length=511, Percent_Identity=26.614481409002, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI157311624, Length=510, Percent_Identity=27.4509803921569, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI157311622, Length=510, Percent_Identity=27.4509803921569, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI42544132, Length=516, Percent_Identity=26.1627906976744, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI122937307, Length=515, Percent_Identity=24.8543689320388, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI38505220, Length=513, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI115511026, Length=521, Percent_Identity=25.5278310940499, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI28416953, Length=133, Percent_Identity=32.3308270676692, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788107, Length=563, Percent_Identity=26.9982238010657, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI221142682, Length=503, Percent_Identity=26.8389662027833, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI145693145, Length=523, Percent_Identity=29.6367112810707, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786810, Length=545, Percent_Identity=26.9724770642202, Blast_Score=128, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=561, Percent_Identity=29.7682709447415, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71994690, Length=563, Percent_Identity=29.8401420959147, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71994694, Length=561, Percent_Identity=29.7682709447415, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17560308, Length=535, Percent_Identity=28.2242990654206, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17559526, Length=512, Percent_Identity=26.7578125, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=500, Percent_Identity=28.6, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17538037, Length=522, Percent_Identity=26.4367816091954, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=524, Percent_Identity=26.5267175572519, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=521, Percent_Identity=26.4875239923225, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=507, Percent_Identity=23.8658777120316, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=530, Percent_Identity=21.3207547169811, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=527, Percent_Identity=24.0986717267552, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=364, Percent_Identity=25.5494505494505, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=498, Percent_Identity=27.3092369477912, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319264, Length=543, Percent_Identity=24.6777163904236, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=138, Percent_Identity=32.6086956521739, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581924, Length=574, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI18859661, Length=511, Percent_Identity=27.5929549902153, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21355181, Length=519, Percent_Identity=27.7456647398844, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19922652, Length=511, Percent_Identity=25.2446183953033, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21356947, Length=500, Percent_Identity=24.2, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648260, Length=500, Percent_Identity=23.4, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=22.0907297830375, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648255, Length=504, Percent_Identity=22.2222222222222, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356441, Length=503, Percent_Identity=21.6699801192843, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24583465, Length=464, Percent_Identity=24.3534482758621, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583463, Length=464, Percent_Identity=24.3534482758621, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364798, Length=464, Percent_Identity=24.3534482758621, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281365686, Length=474, Percent_Identity=21.9409282700422, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20130357, Length=367, Percent_Identity=26.7029972752044, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24653035, Length=504, Percent_Identity=23.4126984126984, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355225, Length=221, Percent_Identity=28.5067873303167, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 59067; Mature: 58936

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGIYDLHLDRNPANFQPLTPLGFLERAAGVFPDHTAIIHGALRRNYRDFYARSRRLAS
CCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALAKLGVGKGDTVAVMLPNAPAMLEAHYGVPMTGAVLNSLNTRLDAAILAFTLDHGEAKV
HHHHHCCCCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCEEEEEEEECCCCEEE
LITDREFSPVMKAALAMATRKPVVIDYDDPEFTGKGERIGTIEYEDFLATGDADFAWKTP
EEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEHHHHEECCCCCCEECCC
DDEWDAIALNYTSGTTGDPKGVVYHHRGAHLLAVGNVVTCSLGKHPVYLWTLPMFHCNGW
CCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCEEEEECCCCEEEEEEEEHHHCCCE
CFPWSITLAAGTHVCLRQVRAKPIFDAIADYKVTHMCGAPIVMSTLLNTPDADKRPLPHK
EEEEEEEEECCHHHHHHHHHCCHHHHHHHCCEEEHHCCCHHHHHHHHCCCCCCCCCCCCE
VEFITAAAPPPEAVLAAMQEAGFNVVHVYGLTEVYGPSVVNEWHAEWDALPAQERAIKKA
EEEEEECCCCHHHHHHHHHHCCCCEEEEEECHHHCCCHHHHHHHHHHCCCCHHHHHHHHH
RQGVRYGALEALDVLDPDTMVPVPADGETLGEVMFRGNVVMKGYLKNPTATDAAFAGGWF
HCCCCCCCHHHHHCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCCE
HSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVAKPDEKWG
ECCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHCCCCCCEEEEEECCCHHHC
ETPVAFVELREGASATAEDLIAHCRTHLAAYKCPRHIVFEEIPKTSTGKIQKFRLREMAK
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
EV
CC
>Mature Secondary Structure 
AGGIYDLHLDRNPANFQPLTPLGFLERAAGVFPDHTAIIHGALRRNYRDFYARSRRLAS
CCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALAKLGVGKGDTVAVMLPNAPAMLEAHYGVPMTGAVLNSLNTRLDAAILAFTLDHGEAKV
HHHHHCCCCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCEEEEEEEECCCCEEE
LITDREFSPVMKAALAMATRKPVVIDYDDPEFTGKGERIGTIEYEDFLATGDADFAWKTP
EEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEHHHHEECCCCCCEECCC
DDEWDAIALNYTSGTTGDPKGVVYHHRGAHLLAVGNVVTCSLGKHPVYLWTLPMFHCNGW
CCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCEEEEECCCCEEEEEEEEHHHCCCE
CFPWSITLAAGTHVCLRQVRAKPIFDAIADYKVTHMCGAPIVMSTLLNTPDADKRPLPHK
EEEEEEEEECCHHHHHHHHHCCHHHHHHHCCEEEHHCCCHHHHHHHHCCCCCCCCCCCCE
VEFITAAAPPPEAVLAAMQEAGFNVVHVYGLTEVYGPSVVNEWHAEWDALPAQERAIKKA
EEEEEECCCCHHHHHHHHHHCCCCEEEEEECHHHCCCHHHHHHHHHHCCCCHHHHHHHHH
RQGVRYGALEALDVLDPDTMVPVPADGETLGEVMFRGNVVMKGYLKNPTATDAAFAGGWF
HCCCCCCCHHHHHCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCCE
HSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVAKPDEKWG
ECCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHCCCCCCEEEEEECCCHHHC
ETPVAFVELREGASATAEDLIAHCRTHLAAYKCPRHIVFEEIPKTSTGKIQKFRLREMAK
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
EV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]