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The map label for this gene is ispH

Identifier: 154246297

GI number: 154246297

Start: 2628764

End: 2629771

Strand: Direct

Name: ispH

Synonym: Xaut_2355

Alternate gene names: 154246297

Gene position: 2628764-2629771 (Clockwise)

Preceding gene: 154246295

Following gene: 154246298

Centisome position: 49.52

GC content: 68.35

Gene sequence:

>1008_bases
ATGCCCTCGATCGAGCCCGCCATGGACCCCGCTGCAGCGCCGGATGCCGCTGCAAAGCCGCCGCTCAAGATCCTTCTTGC
CGCGCCGCGCGGCTTCTGCGCCGGCGTGGTGCGGGCCATCGACGCGGTGGAGCAGGCGCTGAAGCTCTATGGCGCCCCCG
TCTATGTGCGCCACGAGATCGTGCACAACAAATACGTGGTGGAAGATCTCAAGCGGAAGGGCGCCATCTTCGTGGAGGAG
CTGGACGAGGTGCCGGACACCTCCGCGCCGGTTATCTTCTCCGCCCACGGGGTGCCGAAATCGGTGCCGGACGAGGCGAC
CTCCCGCAAGCTGTTCGCCATCGACGCCACCTGCCCGCTGGTGACCAAGGTGCACCGGGAGGCGCAGGTGCATTTCCGCA
AGGGCCGGCACATCCTGCTGATCGGCCATGAGGGCCATCCGGAGGTGGTGGGCACCATCGGCCAGCTGCCGGACGGCGCC
TTCACCCTGATCGAGACCGCCGAGGATGCGCGCAAGGTGATGCCGCGCGACCCGGACAACCTCGCCTATGTGACTCAGAC
CACCCTGTCGCTGGACGACACGGCGGAAATGATCGCCATCCTGCGCGAGCGCTTCCCGGCGCTGAGCGAGCCGCACAAGG
AAGACATCTGCTACGCCACCACCAACCGGCAGGAAGCGGTGAAGGCCATCGCGCCCAGGGTGGATGCGCTGGTTGTGGTG
GGCGCGCCCAATTCCTCCAATTCCCAGCGCCTGCGCGAGGCGGCGGAGCGGGCCGGCTGCGCCCGCGCCGTGCTGGTGCA
GCGGGCGGCGGACATCGACTGGACGGTGTTCTCCAGCATCGCCAGCCTCGGCGTCACCGCCGGCGCCTCGGCTCCGGAGG
TGCTGGTGGAAGAGATCATCGATGCCTTCGTCGAACGCTTCACCGTGACGGTCGAGACTGTGACCTCAACGGAGGAGGAC
GTGTTCTTCCCCCTGCCGCGCGCCCTGCGGCCGGATGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GAGGTCCGCAAATCGGGCGGTTCCGCAACGGCGCCCCGAAATCGCCGGCGCGCCGTTGCGCTCCCTTGACAGGCGCGTCC
CAAGACCGCCAATTCGCCGG

Downstream 100 bases:

>100_bases
AACGTGGCGGTCTATACGGACGTTGCGCCGGCGGAGCTTGAGGCTTTCCTGTCCGGCTACGACATCGGCACGCTCACCTC
TTTCCACGGCATCGCGGAAG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPSIEPAMDPAAAPDAAAKPPLKILLAAPRGFCAGVVRAIDAVEQALKLYGAPVYVRHEIVHNKYVVEDLKRKGAIFVEE
LDEVPDTSAPVIFSAHGVPKSVPDEATSRKLFAIDATCPLVTKVHREAQVHFRKGRHILLIGHEGHPEVVGTIGQLPDGA
FTLIETAEDARKVMPRDPDNLAYVTQTTLSLDDTAEMIAILRERFPALSEPHKEDICYATTNRQEAVKAIAPRVDALVVV
GAPNSSNSQRLREAAERAGCARAVLVQRAADIDWTVFSSIASLGVTAGASAPEVLVEEIIDAFVERFTVTVETVTSTEED
VFFPLPRALRPDAAE

Sequences:

>Translated_335_residues
MPSIEPAMDPAAAPDAAAKPPLKILLAAPRGFCAGVVRAIDAVEQALKLYGAPVYVRHEIVHNKYVVEDLKRKGAIFVEE
LDEVPDTSAPVIFSAHGVPKSVPDEATSRKLFAIDATCPLVTKVHREAQVHFRKGRHILLIGHEGHPEVVGTIGQLPDGA
FTLIETAEDARKVMPRDPDNLAYVTQTTLSLDDTAEMIAILRERFPALSEPHKEDICYATTNRQEAVKAIAPRVDALVVV
GAPNSSNSQRLREAAERAGCARAVLVQRAADIDWTVFSSIASLGVTAGASAPEVLVEEIIDAFVERFTVTVETVTSTEED
VFFPLPRALRPDAAE
>Mature_334_residues
PSIEPAMDPAAAPDAAAKPPLKILLAAPRGFCAGVVRAIDAVEQALKLYGAPVYVRHEIVHNKYVVEDLKRKGAIFVEEL
DEVPDTSAPVIFSAHGVPKSVPDEATSRKLFAIDATCPLVTKVHREAQVHFRKGRHILLIGHEGHPEVVGTIGQLPDGAF
TLIETAEDARKVMPRDPDNLAYVTQTTLSLDDTAEMIAILRERFPALSEPHKEDICYATTNRQEAVKAIAPRVDALVVVG
APNSSNSQRLREAAERAGCARAVLVQRAADIDWTVFSSIASLGVTAGASAPEVLVEEIIDAFVERFTVTVETVTSTEEDV
FFPLPRALRPDAAE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=316, Percent_Identity=51.5822784810127, Blast_Score=324, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 36203; Mature: 36071

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIEPAMDPAAAPDAAAKPPLKILLAAPRGFCAGVVRAIDAVEQALKLYGAPVYVRHEI
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
VHNKYVVEDLKRKGAIFVEELDEVPDTSAPVIFSAHGVPKSVPDEATSRKLFAIDATCPL
HHHHHHHHHHHHCCCEEHHHHHHCCCCCCCEEEEECCCCCCCCCHHHCCEEEEEECCCHH
VTKVHREAQVHFRKGRHILLIGHEGHPEVVGTIGQLPDGAFTLIETAEDARKVMPRDPDN
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHCHHHHHHCCCCCCCC
LAYVTQTTLSLDDTAEMIAILRERFPALSEPHKEDICYATTNRQEAVKAIAPRVDALVVV
EEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEEE
GAPNSSNSQRLREAAERAGCARAVLVQRAADIDWTVFSSIASLGVTAGASAPEVLVEEII
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
DAFVERFTVTVETVTSTEEDVFFPLPRALRPDAAE
HHHHHHHEEEEEEECCCCCCEEECCCHHCCCCCCC
>Mature Secondary Structure 
PSIEPAMDPAAAPDAAAKPPLKILLAAPRGFCAGVVRAIDAVEQALKLYGAPVYVRHEI
CCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
VHNKYVVEDLKRKGAIFVEELDEVPDTSAPVIFSAHGVPKSVPDEATSRKLFAIDATCPL
HHHHHHHHHHHHCCCEEHHHHHHCCCCCCCEEEEECCCCCCCCCHHHCCEEEEEECCCHH
VTKVHREAQVHFRKGRHILLIGHEGHPEVVGTIGQLPDGAFTLIETAEDARKVMPRDPDN
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHCHHHHHHCCCCCCCC
LAYVTQTTLSLDDTAEMIAILRERFPALSEPHKEDICYATTNRQEAVKAIAPRVDALVVV
EEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEEE
GAPNSSNSQRLREAAERAGCARAVLVQRAADIDWTVFSSIASLGVTAGASAPEVLVEEII
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
DAFVERFTVTVETVTSTEEDVFFPLPRALRPDAAE
HHHHHHHEEEEEEECCCCCCEEECCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA