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The map label for this gene is pinE [H]

Identifier: 154246187

GI number: 154246187

Start: 2506326

End: 2506877

Strand: Direct

Name: pinE [H]

Synonym: Xaut_2245

Alternate gene names: 154246187

Gene position: 2506326-2506877 (Clockwise)

Preceding gene: 154246186

Following gene: 154246192

Centisome position: 47.21

GC content: 62.32

Gene sequence:

>552_bases
ATGATCATCGGCTATGCACGCGTATCTACTGATGGACAGACGCTAGACAGCCAGATCGAACACCTCAAAACCTCAGGTTG
CCAGAAAGTCTTTTCTGAGACGGCCAGCGGCGCAAAGACCGATCGGCGTGAGCTGCGCCGCTGCATCGCTTCGCTCGCTG
CCGGCGATGTTCTCGTCGTCGTTCGGCTCGATCGCCTTGCCAGGTCCACGCGGGATCTGCTCAACGTGCTTGCCGAGGTC
GCTGAACGCCACGCCGCATTCCGCTCGCTCTCTGACGCCTGGGCCGATACCACCACGCCCCATGGGCGCCTCATGCTGAC
AGTGCTCGGCGGACTGGCCGAATTCGAGCGCGAACTCATCAAGACGCGAACGGGGGAAGGGAGGGCACGCGCCCAGGCGC
GAGGCGTGAAGTTCGGCCGCAAGAGCGTGCTTACGGTCGATCAGCGCGCCTATGTCGCCAAGTTGCGCGCCGAAGGCGAA
AGCGTGCGACATATCGCCCGCATCATGGGTGTCAGCAAGGCGACCATTAGCCGGATTGCTCCAATCTCCTAA

Upstream 100 bases:

>100_bases
ATATTCGGTGCGACTCAAAACTCAAAATATTTGAGACACTCGGCGGCGCGAGATAAATCAACGACTTGTGTGTCTCGAAT
TATAGTCTCATAATGTGCGC

Downstream 100 bases:

>100_bases
GGGAATGGTAGCGCCAGGACCGGGCCTTGGCATTTGTCCAGAAAGCAGACACTGCCGCGGGCAGTCCCGGATTCTTGCCG
GAAGCTTCGCGCTGAGTGGT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MIIGYARVSTDGQTLDSQIEHLKTSGCQKVFSETASGAKTDRRELRRCIASLAAGDVLVVVRLDRLARSTRDLLNVLAEV
AERHAAFRSLSDAWADTTTPHGRLMLTVLGGLAEFERELIKTRTGEGRARAQARGVKFGRKSVLTVDQRAYVAKLRAEGE
SVRHIARIMGVSKATISRIAPIS

Sequences:

>Translated_183_residues
MIIGYARVSTDGQTLDSQIEHLKTSGCQKVFSETASGAKTDRRELRRCIASLAAGDVLVVVRLDRLARSTRDLLNVLAEV
AERHAAFRSLSDAWADTTTPHGRLMLTVLGGLAEFERELIKTRTGEGRARAQARGVKFGRKSVLTVDQRAYVAKLRAEGE
SVRHIARIMGVSKATISRIAPIS
>Mature_183_residues
MIIGYARVSTDGQTLDSQIEHLKTSGCQKVFSETASGAKTDRRELRRCIASLAAGDVLVVVRLDRLARSTRDLLNVLAEV
AERHAAFRSLSDAWADTTTPHGRLMLTVLGGLAEFERELIKTRTGEGRARAQARGVKFGRKSVLTVDQRAYVAKLRAEGE
SVRHIARIMGVSKATISRIAPIS

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=181, Percent_Identity=43.646408839779, Blast_Score=137, Evalue=3e-34,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=34.4262295081967, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=34.4262295081967, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20014; Mature: 20014

Theoretical pI: Translated: 11.10; Mature: 11.10

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGYARVSTDGQTLDSQIEHLKTSGCQKVFSETASGAKTDRRELRRCIASLAAGDVLVV
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHH
VRLDRLARSTRDLLNVLAEVAERHAAFRSLSDAWADTTTPHGRLMLTVLGGLAEFERELI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KTRTGEGRARAQARGVKFGRKSVLTVDQRAYVAKLRAEGESVRHIARIMGVSKATISRIA
HHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHC
PIS
CCC
>Mature Secondary Structure
MIIGYARVSTDGQTLDSQIEHLKTSGCQKVFSETASGAKTDRRELRRCIASLAAGDVLVV
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHH
VRLDRLARSTRDLLNVLAEVAERHAAFRSLSDAWADTTTPHGRLMLTVLGGLAEFERELI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KTRTGEGRARAQARGVKFGRKSVLTVDQRAYVAKLRAEGESVRHIARIMGVSKATISRIA
HHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHC
PIS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]