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The map label for this gene is amtB [H]

Identifier: 154246151

GI number: 154246151

Start: 2470261

End: 2471757

Strand: Reverse

Name: amtB [H]

Synonym: Xaut_2208

Alternate gene names: 154246151

Gene position: 2471757-2470261 (Counterclockwise)

Preceding gene: 154246152

Following gene: 154246150

Centisome position: 46.56

GC content: 68.4

Gene sequence:

>1497_bases
ATGACGTCCAAGACGTGGTCCCGCGTGGGGATTCCCGCGCTTCTCCTGGCAACGGCATTCGCCTTGCCGGTGCTGGCCCA
GACGGCCGCACCTGCCGCCGATGCCACCACGACCGCCGCTGCCGCCGCGGCGGCCCCCACCGTGAACAAGGGCGACGTGG
CGTGGATGCTCACGTCCTCGCTGCTCGTGCTCATGATGTCGGTGCCCGGCCTGTTCCTGTTCTACGGTGGCCTCCTGCGC
GCCAAGAACATGCTGTCCATGCTCATGCAGGTCGGCTACTGCGTGGCCATCGTCGGCCTCATCTGGGTGCTGTACGGCTA
CTCCCTCGCCTTCACCGGCGGCTCGCCCTTCATCGGCGGCTTCTCCAAGGCGTTCCTGTCGGGCGTGACCGGCGAATCGC
TGGCCGCCACCTTCTCGGTGGGCGTCGCCATTCCCGAGCTGGTGTACATCGCGTTCCAGATGACCTTCGCGATGATCACC
CCCGCGCTGATCTTCGGCGCGTTCGCGGAGCGCACCAAGTTCACCGCCGTGGTGCTGTTCGTGCCGCTGTGGGTGACCTT
CGTGTACTTCCCGGTCGCGCACATGGTGTGGTACTGGGCCGGCCCGGATGCCATCGACGCCGCCGCCAAGGCCGTGGCTG
CCGCCGCTGACGGCGCCGCCAAGACCGCCGCCCAGGCCGCCCTCGACAGCGTGCTGGCCGACGCCGGCTACATCTTCTCC
CTCGGCGCCATCGACTTCGCCGGCGGCACCGTGGTGCACATCAATGCCGGTATCGCCGGCCTCGTCGCCTGCCTCATGGT
CGGCAAGCGCCTGGGCTACGGCAAGGAGGCGCTCTCGCCCCACTCGCTGACCCTCACCATGGTCGGCGCCTCCCTCCTGT
GGGTCGGCTGGTTCGGCTTCAACGCCGGCTCCAACCTCGAGGCCAACGCGGTCACCGCGCTCGCCATGATGAACACCTTC
ATCGCCACCTGCGCCGCGGCCATCTCCTGGATGTTCGTGGAATGGCTGGTGAAGGGTAAGCCCTCCATGCTGGGCCTCGC
CTCGGGCATCGTCGCCGGCCTCGTGGCCGTCACGCCGCCGTCGGGCTTCGCCGGCATCATGGGCTCGCTGGTGCTGGGCC
TCATCGTCAGCCCCATCTGCTTCATCTTCTGCACGGCCATCAAGAACGCGTTCGGCTATGACGACGCGCTCGACGTGTTC
GGCGTGCATGGCGTGGGCGGCATCGTCGGCGCCATCGCCACCGGCATCCTGGTGAGCCCGGCCCTCGGCGGCACCGGCAT
CTTCGACTACACCACCGGTGCCGTGGCCCCCTACGACATGGCCACCCAGGTCATCGCCCAGCTCAAGGCCGTCGGCGTCA
CCGTCGTGTGGTCGGGTGTCGGCACCGCGGTGATCTTGTTCATCATCTCCGTGATCGTCGGCCTGCGTCCCACCACCGAC
GTGGAGCGCGAGGGCCTCGACATCAACGAGCACGGCGAGCGCGCCTACCACTCGTGA

Upstream 100 bases:

>100_bases
AGATTTTCGTCCTTCCGATCGAGCACGCCGTGCGCATCCGCACGGGCGAGACCGACGCCGACGCGCTTTGAGCCTTTCTC
ATTCGACGGAGCTTTCATCC

Downstream 100 bases:

>100_bases
TCTTTCCGGGACGGCGCGCCTTCGGGTGCGCCGCCCCGCCGGTATAAAAGAGGGGAACGAAGAAGAGCCTCGGCACCTTG
GTGCCGGGGCTTTTTTCATG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTSKTWSRVGIPALLLATAFALPVLAQTAAPAADATTTAAAAAAAPTVNKGDVAWMLTSSLLVLMMSVPGLFLFYGGLLR
AKNMLSMLMQVGYCVAIVGLIWVLYGYSLAFTGGSPFIGGFSKAFLSGVTGESLAATFSVGVAIPELVYIAFQMTFAMIT
PALIFGAFAERTKFTAVVLFVPLWVTFVYFPVAHMVWYWAGPDAIDAAAKAVAAAADGAAKTAAQAALDSVLADAGYIFS
LGAIDFAGGTVVHINAGIAGLVACLMVGKRLGYGKEALSPHSLTLTMVGASLLWVGWFGFNAGSNLEANAVTALAMMNTF
IATCAAAISWMFVEWLVKGKPSMLGLASGIVAGLVAVTPPSGFAGIMGSLVLGLIVSPICFIFCTAIKNAFGYDDALDVF
GVHGVGGIVGAIATGILVSPALGGTGIFDYTTGAVAPYDMATQVIAQLKAVGVTVVWSGVGTAVILFIISVIVGLRPTTD
VEREGLDINEHGERAYHS

Sequences:

>Translated_498_residues
MTSKTWSRVGIPALLLATAFALPVLAQTAAPAADATTTAAAAAAAPTVNKGDVAWMLTSSLLVLMMSVPGLFLFYGGLLR
AKNMLSMLMQVGYCVAIVGLIWVLYGYSLAFTGGSPFIGGFSKAFLSGVTGESLAATFSVGVAIPELVYIAFQMTFAMIT
PALIFGAFAERTKFTAVVLFVPLWVTFVYFPVAHMVWYWAGPDAIDAAAKAVAAAADGAAKTAAQAALDSVLADAGYIFS
LGAIDFAGGTVVHINAGIAGLVACLMVGKRLGYGKEALSPHSLTLTMVGASLLWVGWFGFNAGSNLEANAVTALAMMNTF
IATCAAAISWMFVEWLVKGKPSMLGLASGIVAGLVAVTPPSGFAGIMGSLVLGLIVSPICFIFCTAIKNAFGYDDALDVF
GVHGVGGIVGAIATGILVSPALGGTGIFDYTTGAVAPYDMATQVIAQLKAVGVTVVWSGVGTAVILFIISVIVGLRPTTD
VEREGLDINEHGERAYHS
>Mature_497_residues
TSKTWSRVGIPALLLATAFALPVLAQTAAPAADATTTAAAAAAAPTVNKGDVAWMLTSSLLVLMMSVPGLFLFYGGLLRA
KNMLSMLMQVGYCVAIVGLIWVLYGYSLAFTGGSPFIGGFSKAFLSGVTGESLAATFSVGVAIPELVYIAFQMTFAMITP
ALIFGAFAERTKFTAVVLFVPLWVTFVYFPVAHMVWYWAGPDAIDAAAKAVAAAADGAAKTAAQAALDSVLADAGYIFSL
GAIDFAGGTVVHINAGIAGLVACLMVGKRLGYGKEALSPHSLTLTMVGASLLWVGWFGFNAGSNLEANAVTALAMMNTFI
ATCAAAISWMFVEWLVKGKPSMLGLASGIVAGLVAVTPPSGFAGIMGSLVLGLIVSPICFIFCTAIKNAFGYDDALDVFG
VHGVGGIVGAIATGILVSPALGGTGIFDYTTGAVAPYDMATQVIAQLKAVGVTVVWSGVGTAVILFIISVIVGLRPTTDV
EREGLDINEHGERAYHS

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=456, Percent_Identity=44.7368421052632, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17550182, Length=479, Percent_Identity=27.9749478079332, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17550180, Length=471, Percent_Identity=26.7515923566879, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32564042, Length=473, Percent_Identity=25.1585623678647, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI133931100, Length=469, Percent_Identity=24.9466950959488, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325396, Length=461, Percent_Identity=31.8872017353579, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321559, Length=450, Percent_Identity=30.4444444444444, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324187, Length=463, Percent_Identity=30.2375809935205, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI161078314, Length=467, Percent_Identity=26.1241970021413, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51179; Mature: 51047

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSKTWSRVGIPALLLATAFALPVLAQTAAPAADATTTAAAAAAAPTVNKGDVAWMLTSS
CCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHH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HHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]