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The map label for this gene is yhfT [H]

Identifier: 154245949

GI number: 154245949

Start: 2261503

End: 2262966

Strand: Direct

Name: yhfT [H]

Synonym: Xaut_2006

Alternate gene names: 154245949

Gene position: 2261503-2262966 (Clockwise)

Preceding gene: 154245948

Following gene: 154245950

Centisome position: 42.6

GC content: 73.02

Gene sequence:

>1464_bases
GTGAGCATCACCCGCCATCTCGCCCGCCACGGCGCCGAGCGCCCCGACGCGCCGGCCCTGACCTGCGAGGGCGAAAGCCT
CACCTATGGCTCCCTCGCCGCGCTGGTGCAGCGCATCGCCGCCCGGTTCAGGGACGCGCCGGACGGCGGCATCGCGCTCG
ACCTGCCCAATGGGGCGGCGCTGGCGGCGCTGTTCCTCGCCGCCGCCCATGCGGGACGGGAGGGGCAGGTGCTCGACCCC
GCCTGGCCGGTGGGGCAGCGGGCGGACATCCTCGCCCGCATCCGGCCGGGGCTGCTGGTGTCGTGCGGTGCCGACGCGGA
TGTGCGCCTGTCCCCGGCCCAGGGACTGGCGGGCCTTGCCGAGGCCATGGGCGCCGGCGCGGCGGAGGTGACCGGGGCGC
CGGACCCGGACCTGCCCTTCTATGTGGGCTTCACCTCCGGCTCCACCGGCCTGCCCAAGGGCTATCGCCGCGCCCACCGC
TCCTGGACCGAGAGCTTCGATGCCGACACCCGCGAATTCGGCATCGGCCCCGATGACGTGGTGCTGGCGCCGGGGGCGCT
CACCCATTCCCTTTTCCTCTATGCGCTGGCGCGGGGCCTCGATGCCGGTGCCCATGTGATCCTGTCGAAGAGCTTCCGCC
CCCGCGCGGTGGCGGAGCTGGCGCGCCGGCACACGGCCAGCGTGCTCTATGGCGTGCCCACCCAGCTCGCCTTGCTGCTG
GATCATCTTGCGGCGGAGGGCGCGCGGCTGGACGGCATCCGCCTCGTGCTGTGCTCGGGCGCCAAATGGCCGGCGGGGCG
CAAGGCGCTGCTTTCCCGCCACCTGCCCAAGGCCCGTTTCGCCGAATTCTACGGCGCGTCGGAACTCTCCTTCATCACTG
TCGCGAAGGGCGAGGAGCCGGTGCCCGAGGGCTCCGTGGGCCGGGCCTTCGACGGCGTGCGCCTCGCCATCCGCGACAAG
GCGGGCCGGCGCCTGCCCATGGGGCGCATCGGCCTCGTGTTCGTGGAGAGCCCCTTCCTGTTCCAGGACTATGCCACCGG
CGACAGCGCCGACCTGCTGCGCCATGGCCCGGAAATCAGCGTCGGCGACATGGGCCGGCTGGACGCCGCCGGCTTCCTCA
CCCTGGCGGGGCGGGCGAAGCGCATGATCGTCACCTCCGGCAAGAACCTCTATCCCGAAGAGGTGGAGCGGCAGCTGGAG
CTGCACCCCGCCATCGCCACCGCCGCCGTGATGGGCGTGCCCGATGGCAAGCGCGGCGAGCGGCTGGTGGCCTTCATCCA
ACCGGAGGACGGGGCGCAGATGACGCGGGCGGATCTGGTGGCGTGGCTGCGGCCGCGCCTCGCCCTGTTCAAGGTGCCGC
GGCTTTATGCGCGGGTCGCCCGCTGGCCGCTGACGGCCTCCGGCAAGACCGATTTTGCCGCCGTCGCCCGCCTGTGGCCG
CACGATTGCGAGCTGATCCCATGA

Upstream 100 bases:

>100_bases
CGGCGGTGTTCCTGCCGGGCGACCTGCTCAAGGCGGTGGGCGCTTCGGTGCTGGTCACCACCGTGCTGCGCAACTGGTCG
GTGGCGGCGCAGCCGCGCCC

Downstream 100 bases:

>100_bases
CCGGCGTCGCCCTTCTGGCCGCCAGGCGCACGGCGGTGGTGCCGCGCGGCGGCGCCTTCCGCGACATGGAGCCCTTCGCC
CTGGCGGCGGCCCTGATCGG

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSITRHLARHGAERPDAPALTCEGESLTYGSLAALVQRIAARFRDAPDGGIALDLPNGAALAALFLAAAHAGREGQVLDP
AWPVGQRADILARIRPGLLVSCGADADVRLSPAQGLAGLAEAMGAGAAEVTGAPDPDLPFYVGFTSGSTGLPKGYRRAHR
SWTESFDADTREFGIGPDDVVLAPGALTHSLFLYALARGLDAGAHVILSKSFRPRAVAELARRHTASVLYGVPTQLALLL
DHLAAEGARLDGIRLVLCSGAKWPAGRKALLSRHLPKARFAEFYGASELSFITVAKGEEPVPEGSVGRAFDGVRLAIRDK
AGRRLPMGRIGLVFVESPFLFQDYATGDSADLLRHGPEISVGDMGRLDAAGFLTLAGRAKRMIVTSGKNLYPEEVERQLE
LHPAIATAAVMGVPDGKRGERLVAFIQPEDGAQMTRADLVAWLRPRLALFKVPRLYARVARWPLTASGKTDFAAVARLWP
HDCELIP

Sequences:

>Translated_487_residues
MSITRHLARHGAERPDAPALTCEGESLTYGSLAALVQRIAARFRDAPDGGIALDLPNGAALAALFLAAAHAGREGQVLDP
AWPVGQRADILARIRPGLLVSCGADADVRLSPAQGLAGLAEAMGAGAAEVTGAPDPDLPFYVGFTSGSTGLPKGYRRAHR
SWTESFDADTREFGIGPDDVVLAPGALTHSLFLYALARGLDAGAHVILSKSFRPRAVAELARRHTASVLYGVPTQLALLL
DHLAAEGARLDGIRLVLCSGAKWPAGRKALLSRHLPKARFAEFYGASELSFITVAKGEEPVPEGSVGRAFDGVRLAIRDK
AGRRLPMGRIGLVFVESPFLFQDYATGDSADLLRHGPEISVGDMGRLDAAGFLTLAGRAKRMIVTSGKNLYPEEVERQLE
LHPAIATAAVMGVPDGKRGERLVAFIQPEDGAQMTRADLVAWLRPRLALFKVPRLYARVARWPLTASGKTDFAAVARLWP
HDCELIP
>Mature_486_residues
SITRHLARHGAERPDAPALTCEGESLTYGSLAALVQRIAARFRDAPDGGIALDLPNGAALAALFLAAAHAGREGQVLDPA
WPVGQRADILARIRPGLLVSCGADADVRLSPAQGLAGLAEAMGAGAAEVTGAPDPDLPFYVGFTSGSTGLPKGYRRAHRS
WTESFDADTREFGIGPDDVVLAPGALTHSLFLYALARGLDAGAHVILSKSFRPRAVAELARRHTASVLYGVPTQLALLLD
HLAAEGARLDGIRLVLCSGAKWPAGRKALLSRHLPKARFAEFYGASELSFITVAKGEEPVPEGSVGRAFDGVRLAIRDKA
GRRLPMGRIGLVFVESPFLFQDYATGDSADLLRHGPEISVGDMGRLDAAGFLTLAGRAKRMIVTSGKNLYPEEVERQLEL
HPAIATAAVMGVPDGKRGERLVAFIQPEDGAQMTRADLVAWLRPRLALFKVPRLYARVARWPLTASGKTDFAAVARLWPH
DCELIP

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=343, Percent_Identity=26.2390670553936, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI42544132, Length=528, Percent_Identity=24.2424242424242, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI115511026, Length=494, Percent_Identity=24.6963562753036, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI38505220, Length=347, Percent_Identity=27.3775216138329, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI157311624, Length=349, Percent_Identity=26.0744985673352, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI157311622, Length=349, Percent_Identity=26.0744985673352, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI58082049, Length=355, Percent_Identity=25.3521126760563, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI187761345, Length=372, Percent_Identity=24.7311827956989, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI187761343, Length=372, Percent_Identity=24.7311827956989, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI122937307, Length=201, Percent_Identity=30.3482587064677, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI145693145, Length=494, Percent_Identity=26.1133603238866, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788107, Length=342, Percent_Identity=27.4853801169591, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786810, Length=396, Percent_Identity=26.2626262626263, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790505, Length=381, Percent_Identity=25.1968503937008, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI221142682, Length=375, Percent_Identity=24.5333333333333, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789201, Length=374, Percent_Identity=28.3422459893048, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786801, Length=512, Percent_Identity=24.8046875, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71994690, Length=356, Percent_Identity=27.247191011236, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=356, Percent_Identity=27.247191011236, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71994703, Length=356, Percent_Identity=27.247191011236, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=464, Percent_Identity=25.8620689655172, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=404, Percent_Identity=26.2376237623762, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17560308, Length=372, Percent_Identity=26.6129032258064, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=370, Percent_Identity=25.4054054054054, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=349, Percent_Identity=25.214899713467, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=368, Percent_Identity=24.4565217391304, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=326, Percent_Identity=24.8466257668712, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=428, Percent_Identity=21.7289719626168, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=321, Percent_Identity=24.6105919003115, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=343, Percent_Identity=27.6967930029155, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24581924, Length=362, Percent_Identity=27.0718232044199, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24656500, Length=313, Percent_Identity=28.1150159744409, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648260, Length=401, Percent_Identity=24.6882793017456, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922652, Length=285, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130357, Length=335, Percent_Identity=26.2686567164179, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24667959, Length=363, Percent_Identity=25.3443526170799, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62472339, Length=363, Percent_Identity=25.3443526170799, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24667955, Length=363, Percent_Identity=25.3443526170799, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356947, Length=306, Percent_Identity=22.2222222222222, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI18859661, Length=332, Percent_Identity=24.6987951807229, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648676, Length=485, Percent_Identity=23.5051546391753, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356441, Length=306, Percent_Identity=21.5686274509804, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648255, Length=315, Percent_Identity=20.952380952381, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355181, Length=318, Percent_Identity=22.6415094339623, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281365686, Length=314, Percent_Identity=22.9299363057325, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648253, Length=306, Percent_Identity=20.9150326797386, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653035, Length=307, Percent_Identity=22.8013029315961, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 51877; Mature: 51746

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITRHLARHGAERPDAPALTCEGESLTYGSLAALVQRIAARFRDAPDGGIALDLPNGAA
CCHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHH
LAALFLAAAHAGREGQVLDPAWPVGQRADILARIRPGLLVSCGADADVRLSPAQGLAGLA
HHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHCCCCEEEEECCCCCEEECCHHHHHHHH
EAMGAGAAEVTGAPDPDLPFYVGFTSGSTGLPKGYRRAHRSWTESFDADTREFGIGPDDV
HHHCCCCEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCE
VLAPGALTHSLFLYALARGLDAGAHVILSKSFRPRAVAELARRHTASVLYGVPTQLALLL
EECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
DHLAAEGARLDGIRLVLCSGAKWPAGRKALLSRHLPKARFAEFYGASELSFITVAKGEEP
HHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCC
VPEGSVGRAFDGVRLAIRDKAGRRLPMGRIGLVFVESPFLFQDYATGDSADLLRHGPEIS
CCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHCCCCCC
VGDMGRLDAAGFLTLAGRAKRMIVTSGKNLYPEEVERQLELHPAIATAAVMGVPDGKRGE
CCCCCCCCCCHHEEECCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHEECCCCCCCCC
RLVAFIQPEDGAQMTRADLVAWLRPRLALFKVPRLYARVARWPLTASGKTDFAAVARLWP
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
HDCELIP
CCCCCCC
>Mature Secondary Structure 
SITRHLARHGAERPDAPALTCEGESLTYGSLAALVQRIAARFRDAPDGGIALDLPNGAA
CHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHH
LAALFLAAAHAGREGQVLDPAWPVGQRADILARIRPGLLVSCGADADVRLSPAQGLAGLA
HHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHCCCCEEEEECCCCCEEECCHHHHHHHH
EAMGAGAAEVTGAPDPDLPFYVGFTSGSTGLPKGYRRAHRSWTESFDADTREFGIGPDDV
HHHCCCCEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCE
VLAPGALTHSLFLYALARGLDAGAHVILSKSFRPRAVAELARRHTASVLYGVPTQLALLL
EECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
DHLAAEGARLDGIRLVLCSGAKWPAGRKALLSRHLPKARFAEFYGASELSFITVAKGEEP
HHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCC
VPEGSVGRAFDGVRLAIRDKAGRRLPMGRIGLVFVESPFLFQDYATGDSADLLRHGPEIS
CCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHCCCCCC
VGDMGRLDAAGFLTLAGRAKRMIVTSGKNLYPEEVERQLELHPAIATAAVMGVPDGKRGE
CCCCCCCCCCHHEEECCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHEECCCCCCCCC
RLVAFIQPEDGAQMTRADLVAWLRPRLALFKVPRLYARVARWPLTASGKTDFAAVARLWP
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
HDCELIP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]