Click here to switch to the map view.

The map label for this gene is wcaJ [H]

Identifier: 154245894

GI number: 154245894

Start: 2206667

End: 2208085

Strand: Direct

Name: wcaJ [H]

Synonym: Xaut_1951

Alternate gene names: 154245894

Gene position: 2206667-2208085 (Clockwise)

Preceding gene: 154245893

Following gene: 154245895

Centisome position: 41.57

GC content: 67.09

Gene sequence:

>1419_bases
ATGACACGTTGGACCCACGGGCTCGTCAACCGCCTCGTCCTGTTGTGTGACGTCGCCATGGCGGCGCTGGCCGCCATCAT
TGCCCACGGACGCTGGGATTTCCTGTCCTGGAGCCAGATGGCCATCCTCTGGGCCATCGGCGTCTTCGCCTTCGTCCAGA
TCCTGCAGACCGGCCGCGCCTATCGGGTCGAGCATTACACGCGCCCGCTGCGCCAGCTCGGTCACCTCGCGGTGGCCGGC
GTGCCGGCCGGCGCGCTTCTGGCGATCTGCTACTACGCCATCATCCCCATCGGCTCGACCAACCTCGTTGCGCTGCTCGG
CTGGGGCATCCTGACCGCGCTGACGCTGATCTTCGGCCGCTTCGTGCTGGTGCGGACCGGCGTGGCGCTGGTGAAGACCC
GCGGAGTCCTGCGACGCAATGCGGTGATCATCGGGGATCCGGCCCGGGCCCGCGACCTCGTGCGCCGCTACCGCGAGGAG
GAGGGCGCGAACGGCCTCATCACCTTCGTCGGCATGTTCGACGAGGGACACATGCCGGGCGAGATGACCCCGCCACCCCC
CGCGCCCGACGGCGTTCCCGCGATGGGCGGACTCGCCGACCTGCTGGAATATGTGAAGCACAATCCGGTGGACATCGTGG
TCGTCGCCAAGAGCTGGGACGATCCCGCAGCCATCAGCACCATCGCCAACCAGATGTACCAGATCGCCGCCGACGTCATC
GTCGAGCTGGATCCCGAGCGCTTCATGCTCAACGACGCCCGGATCACGCGCATCGCCGGCGAGCCCGCGCTCCAGGTGCA
GCAGCAGCCACTCAAGGGCTCGCTGGGGCTGCTGAAGGGGGTGGAGGACTATGTGGTGGCCACCATCGGCCTCATCCTCA
CCGCCCCCATCCTGCTGGTCGCCGCCGCCGCCATCAAGCTGGATTCCCCCGGGCCCATCCTGTTCCGCCAGCCGCGCGTG
GGCCTCAACAACCGCGTCTTCTCCTGCTTCAAGCTGCGGACCATGCGGGTCGATCTCTCGGACGACGGCTCCCGCGGCAC
CACCAAGGACGATCCGCGCATCACCCGCATCGGCGCCTTCCTGCGCTCCACCTCCATCGACGAACTGCCCCAGCTCATCA
ATGTGCTGCGGGGCGAGATGTCGGTGGTCGGGCCGCGTCCCCATGTGCCCAACATGCAGGTCGCCACCAACGTGCGCTAC
GAGGCGATCCGGCAATATGTGGCGCGCTACCGGATGAAGCCCGGCATCACCGGCTGGGCCCAGATCAACGGCATGCGCGG
CGGCATCAACACCCTCGAAAAGGCGGAGCGGGGCGTGGAGCTCGACCTTTTCTACATCGAGCACTGGTCCATATGGTTCG
ACATCAGAATCATGCTGCTCACCATCACCAAGGGCATGGCCGGGCCGGCGGTCTTCTGA

Upstream 100 bases:

>100_bases
TTCCCCAGCTTGACTGCCAACGTCTTCTTCCGCAGAACGAATCCCGGTCGCGGCTCGACTCAACGAGGTCGGGCGCGTTC
TGCCGGCAGGGATTGGCGCG

Downstream 100 bases:

>100_bases
CCGTCCTGGTGGCGCTGGTCCTGGTCCTTGGCCTGTCGGCCGGCCACGCTGCGGCGGCGGAGGCAGAGCGACGCCCGTTC
CTCGCCTATCTCGCGTCCTG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTRWTHGLVNRLVLLCDVAMAALAAIIAHGRWDFLSWSQMAILWAIGVFAFVQILQTGRAYRVEHYTRPLRQLGHLAVAG
VPAGALLAICYYAIIPIGSTNLVALLGWGILTALTLIFGRFVLVRTGVALVKTRGVLRRNAVIIGDPARARDLVRRYREE
EGANGLITFVGMFDEGHMPGEMTPPPPAPDGVPAMGGLADLLEYVKHNPVDIVVVAKSWDDPAAISTIANQMYQIAADVI
VELDPERFMLNDARITRIAGEPALQVQQQPLKGSLGLLKGVEDYVVATIGLILTAPILLVAAAAIKLDSPGPILFRQPRV
GLNNRVFSCFKLRTMRVDLSDDGSRGTTKDDPRITRIGAFLRSTSIDELPQLINVLRGEMSVVGPRPHVPNMQVATNVRY
EAIRQYVARYRMKPGITGWAQINGMRGGINTLEKAERGVELDLFYIEHWSIWFDIRIMLLTITKGMAGPAVF

Sequences:

>Translated_472_residues
MTRWTHGLVNRLVLLCDVAMAALAAIIAHGRWDFLSWSQMAILWAIGVFAFVQILQTGRAYRVEHYTRPLRQLGHLAVAG
VPAGALLAICYYAIIPIGSTNLVALLGWGILTALTLIFGRFVLVRTGVALVKTRGVLRRNAVIIGDPARARDLVRRYREE
EGANGLITFVGMFDEGHMPGEMTPPPPAPDGVPAMGGLADLLEYVKHNPVDIVVVAKSWDDPAAISTIANQMYQIAADVI
VELDPERFMLNDARITRIAGEPALQVQQQPLKGSLGLLKGVEDYVVATIGLILTAPILLVAAAAIKLDSPGPILFRQPRV
GLNNRVFSCFKLRTMRVDLSDDGSRGTTKDDPRITRIGAFLRSTSIDELPQLINVLRGEMSVVGPRPHVPNMQVATNVRY
EAIRQYVARYRMKPGITGWAQINGMRGGINTLEKAERGVELDLFYIEHWSIWFDIRIMLLTITKGMAGPAVF
>Mature_471_residues
TRWTHGLVNRLVLLCDVAMAALAAIIAHGRWDFLSWSQMAILWAIGVFAFVQILQTGRAYRVEHYTRPLRQLGHLAVAGV
PAGALLAICYYAIIPIGSTNLVALLGWGILTALTLIFGRFVLVRTGVALVKTRGVLRRNAVIIGDPARARDLVRRYREEE
GANGLITFVGMFDEGHMPGEMTPPPPAPDGVPAMGGLADLLEYVKHNPVDIVVVAKSWDDPAAISTIANQMYQIAADVIV
ELDPERFMLNDARITRIAGEPALQVQQQPLKGSLGLLKGVEDYVVATIGLILTAPILLVAAAAIKLDSPGPILFRQPRVG
LNNRVFSCFKLRTMRVDLSDDGSRGTTKDDPRITRIGAFLRSTSIDELPQLINVLRGEMSVVGPRPHVPNMQVATNVRYE
AIRQYVARYRMKPGITGWAQINGMRGGINTLEKAERGVELDLFYIEHWSIWFDIRIMLLTITKGMAGPAVF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=377, Percent_Identity=38.4615384615385, Blast_Score=253, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51911; Mature: 51780

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRWTHGLVNRLVLLCDVAMAALAAIIAHGRWDFLSWSQMAILWAIGVFAFVQILQTGRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
YRVEHYTRPLRQLGHLAVAGVPAGALLAICYYAIIPIGSTNLVALLGWGILTALTLIFGR
EEHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
FVLVRTGVALVKTRGVLRRNAVIIGDPARARDLVRRYREEEGANGLITFVGMFDEGHMPG
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
EMTPPPPAPDGVPAMGGLADLLEYVKHNPVDIVVVAKSWDDPAAISTIANQMYQIAADVI
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VELDPERFMLNDARITRIAGEPALQVQQQPLKGSLGLLKGVEDYVVATIGLILTAPILLV
EEECCCHHEECCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAIKLDSPGPILFRQPRVGLNNRVFSCFKLRTMRVDLSDDGSRGTTKDDPRITRIGAF
HHHHHEECCCCCEEEECCCCCCCHHHHHEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHH
LRSTSIDELPQLINVLRGEMSVVGPRPHVPNMQVATNVRYEAIRQYVARYRMKPGITGWA
HHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCHH
QINGMRGGINTLEKAERGVELDLFYIEHWSIWFDIRIMLLTITKGMAGPAVF
EECCCCCCHHHHHHHHCCCEEEEEEEEEEEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TRWTHGLVNRLVLLCDVAMAALAAIIAHGRWDFLSWSQMAILWAIGVFAFVQILQTGRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
YRVEHYTRPLRQLGHLAVAGVPAGALLAICYYAIIPIGSTNLVALLGWGILTALTLIFGR
EEHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
FVLVRTGVALVKTRGVLRRNAVIIGDPARARDLVRRYREEEGANGLITFVGMFDEGHMPG
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
EMTPPPPAPDGVPAMGGLADLLEYVKHNPVDIVVVAKSWDDPAAISTIANQMYQIAADVI
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VELDPERFMLNDARITRIAGEPALQVQQQPLKGSLGLLKGVEDYVVATIGLILTAPILLV
EEECCCHHEECCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAIKLDSPGPILFRQPRVGLNNRVFSCFKLRTMRVDLSDDGSRGTTKDDPRITRIGAF
HHHHHEECCCCCEEEECCCCCCCHHHHHEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHH
LRSTSIDELPQLINVLRGEMSVVGPRPHVPNMQVATNVRYEAIRQYVARYRMKPGITGWA
HHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCHH
QINGMRGGINTLEKAERGVELDLFYIEHWSIWFDIRIMLLTITKGMAGPAVF
EECCCCCCHHHHHHHHCCCEEEEEEEEEEEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]