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The map label for this gene is ampR [H]

Identifier: 154244685

GI number: 154244685

Start: 819561

End: 820505

Strand: Reverse

Name: ampR [H]

Synonym: Xaut_0734

Alternate gene names: 154244685

Gene position: 820505-819561 (Counterclockwise)

Preceding gene: 154244690

Following gene: 154244684

Centisome position: 15.46

GC content: 61.8

Gene sequence:

>945_bases
ATGATGCGGCGGCTACCCTCGCTGAACGGACTGCGCGCTTTTGAGGCAACTGGCCGGCACGGCAGTTTCACCGCCGCGGC
AAGAGAGCTGAACGTCACCCAAACAGCGGTAAGCCGCTTGGTCCGTCTGCTGGAGGATCGTCTTGGTTTTCCTCTGTTCC
GGCGACATGCGAACGCGCTTGAGCTAACGGCGCAAGGGCAGGCACTGCTGTCCGGTCTGACCGATGCTTTCGATACCATC
GCCCGGCTCACTGAAAGCGTTACGGCGATGCGTGGTGGCCCGGTGCTAACGGTTGGAGTCGGCCCAACCCTGGCGGTCAC
TTGGCTGATCCCGCGACTGGCACACTTCTATCGCAGCCATCCCGACGTCGAAGTGCGCATGGCGACGGGTGGCGCAACTC
GCCCGGTACGCGATGACTGGACCTGCACTATCCGACGCGATACCGACGCGTTACCGGGCTACAATGCAGAATGCCTATTC
CCGAGCACCGTCGTGCCGGTCTGCACGCCTAAGCTCGCATCGACGCTGCGCTCGCCTGACGACTTGCGCAACGCGACTCT
CATCGTCGTGTCCCACATGCCAAACGAATGGCCACACTGGTTCGCGGCGGCGGGCTTACGCTCCCCCGTGCACCCCGCCG
GTGAAGTGTCATTCGAGAGCAATGTCATGGCGATGCAGGCAGCGCTCGACGGGGTCGGAGTCGCCATCGCTCAAATCCCT
TACGTCAGCGATGCCCTGGCAGCGGGCCGCTTGGTCGCGCCATTCCCGATCGTCGCGCACACAGTCGGGGGTTGGCTTCT
AGAATACCGCGCTATTCGAAAGGAAGATCCGGCACTTCTAGCTTTTCGCGGTTGGTTGCACCAGGAAGCTGATCGGGCGC
GTCAAGTTGAGAACATGCTAGTCAAGCAAGGATCAATAGAACGACGAGAAGGGCCAAGCGCTTGA

Upstream 100 bases:

>100_bases
CCGCACGAAGTCCGATTACTCAAGGTATATTGGGGTGAAGATCATTGTCAGGCAAACGCGTTTAACTTACCGTGTGCCCA
AGAAAAGATTGGGGTCTCAC

Downstream 100 bases:

>100_bases
GGTCTTCAACTTCGCTAAGATTCGCGAACATTGGAAATGACTGCCTCGGATTCGGACATCCCGGGACAGTGGCCCGGTGA
GACGTACTAGAATGTCGTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MMRRLPSLNGLRAFEATGRHGSFTAAARELNVTQTAVSRLVRLLEDRLGFPLFRRHANALELTAQGQALLSGLTDAFDTI
ARLTESVTAMRGGPVLTVGVGPTLAVTWLIPRLAHFYRSHPDVEVRMATGGATRPVRDDWTCTIRRDTDALPGYNAECLF
PSTVVPVCTPKLASTLRSPDDLRNATLIVVSHMPNEWPHWFAAAGLRSPVHPAGEVSFESNVMAMQAALDGVGVAIAQIP
YVSDALAAGRLVAPFPIVAHTVGGWLLEYRAIRKEDPALLAFRGWLHQEADRARQVENMLVKQGSIERREGPSA

Sequences:

>Translated_314_residues
MMRRLPSLNGLRAFEATGRHGSFTAAARELNVTQTAVSRLVRLLEDRLGFPLFRRHANALELTAQGQALLSGLTDAFDTI
ARLTESVTAMRGGPVLTVGVGPTLAVTWLIPRLAHFYRSHPDVEVRMATGGATRPVRDDWTCTIRRDTDALPGYNAECLF
PSTVVPVCTPKLASTLRSPDDLRNATLIVVSHMPNEWPHWFAAAGLRSPVHPAGEVSFESNVMAMQAALDGVGVAIAQIP
YVSDALAAGRLVAPFPIVAHTVGGWLLEYRAIRKEDPALLAFRGWLHQEADRARQVENMLVKQGSIERREGPSA
>Mature_314_residues
MMRRLPSLNGLRAFEATGRHGSFTAAARELNVTQTAVSRLVRLLEDRLGFPLFRRHANALELTAQGQALLSGLTDAFDTI
ARLTESVTAMRGGPVLTVGVGPTLAVTWLIPRLAHFYRSHPDVEVRMATGGATRPVRDDWTCTIRRDTDALPGYNAECLF
PSTVVPVCTPKLASTLRSPDDLRNATLIVVSHMPNEWPHWFAAAGLRSPVHPAGEVSFESNVMAMQAALDGVGVAIAQIP
YVSDALAAGRLVAPFPIVAHTVGGWLLEYRAIRKEDPALLAFRGWLHQEADRARQVENMLVKQGSIERREGPSA

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=35.5172413793103, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=32.3024054982818, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788706, Length=258, Percent_Identity=27.906976744186, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787128, Length=261, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693193, Length=268, Percent_Identity=27.6119402985075, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790262, Length=238, Percent_Identity=27.3109243697479, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789440, Length=273, Percent_Identity=23.4432234432234, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=24.5551601423488, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI157672245, Length=184, Percent_Identity=28.2608695652174, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34225; Mature: 34225

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRLPSLNGLRAFEATGRHGSFTAAARELNVTQTAVSRLVRLLEDRLGFPLFRRHANAL
CCCCCCCCCCCCHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCE
ELTAQGQALLSGLTDAFDTIARLTESVTAMRGGPVLTVGVGPTLAVTWLIPRLAHFYRSH
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCC
PDVEVRMATGGATRPVRDDWTCTIRRDTDALPGYNAECLFPSTVVPVCTPKLASTLRSPD
CCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCHHHCCHHHHHHHCCCH
DLRNATLIVVSHMPNEWPHWFAAAGLRSPVHPAGEVSFESNVMAMQAALDGVGVAIAQIP
HHCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
YVSDALAAGRLVAPFPIVAHTVGGWLLEYRAIRKEDPALLAFRGWLHQEADRARQVENML
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHH
VKQGSIERREGPSA
HHCCCCCCCCCCCC
>Mature Secondary Structure
MMRRLPSLNGLRAFEATGRHGSFTAAARELNVTQTAVSRLVRLLEDRLGFPLFRRHANAL
CCCCCCCCCCCCHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCE
ELTAQGQALLSGLTDAFDTIARLTESVTAMRGGPVLTVGVGPTLAVTWLIPRLAHFYRSH
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCC
PDVEVRMATGGATRPVRDDWTCTIRRDTDALPGYNAECLFPSTVVPVCTPKLASTLRSPD
CCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCHHHCCHHHHHHHCCCH
DLRNATLIVVSHMPNEWPHWFAAAGLRSPVHPAGEVSFESNVMAMQAALDGVGVAIAQIP
HHCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
YVSDALAAGRLVAPFPIVAHTVGGWLLEYRAIRKEDPALLAFRGWLHQEADRARQVENML
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHH
VKQGSIERREGPSA
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8405939; 10984043; 2125210 [H]