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The map label for this gene is metZ [H]

Identifier: 154244428

GI number: 154244428

Start: 514980

End: 516200

Strand: Reverse

Name: metZ [H]

Synonym: Xaut_0471

Alternate gene names: 154244428

Gene position: 516200-514980 (Counterclockwise)

Preceding gene: 154244430

Following gene: 154244427

Centisome position: 9.72

GC content: 68.63

Gene sequence:

>1221_bases
ATGTCCGTGGCGCAAAAGCCAGCCCCCAAATTCATCCCCAAAGATCCCCTTACACTCCACCCGGAGACGCGCCTAGTCCA
TGGCGGTACCACGCGCTCGCCCTATGGGGAGACCTCCGAGGCGCTGTTCCTGACCCAGGGCTTCGTCTACGACAATGCCG
AGTCCTGCGAGGCCCGCTTCAAGGGCGAGGATCCGGGCTTCGTCTATTCCCGCTACGGCAATCCCACCACCGCCATGTTC
GAGGAACGCATGGCGCTGCTGGAGGGCGCCGAGGCGGCCCGGGCCACGGCTTCCGGCATGGCGGCGGTGAACGCGGCGCT
GCTGTGCCAGCTCTCGGCCGGCGACCATGTGGTGGCGGCGCGCGCGCTGTTCGGCTCGTGTCGCTGGATCCTGGAGGACT
TCCTGCCGCGCTTCGGCATCGAGACCACCCTGGTGGACGGCACCGACCTTGCCGCCTGGCAGGCGGCAGCACGGCCGAGC
ACCAAGGTGTTCTTCCTGGAAAGCCCCACCAACCCCACCCTGGAGCTGATCGACATCGCGGCGGTGGCGCAGATCGCCCA
CGCCGCCGGCGCCCGGCTGGTGGTGGACAATGTGTTCGCCACTCCCCTGCTCCAGCACCCGTTCGAGCTGGGCGCGGACA
TCGTGGTCTATTCCACCACCAAGCATGTGGACGGCCAGGGCCGCTGCCTCGGCGGCGTCATCCTCTCCTCCCAGCAGTTC
GTCACTGACCACCTGCAGACCTTCCTGCGCCAGACCGGCCCCTCCATCTCCCCCTTCAACGCCTGGATCATGCTGAAGGG
CCTGGAGACGCTGGCGCTGCGGGTGGAGCGCATGCAGGCGAGCGCCGGCCGCATCGCCGATGCTCTGGCCGGCCTGCCCA
AGATCGAGACCGTGCTCTATCCCGGCCGCGCCGACCATCCGCAGGCCGAGCTGGCCCGCCGGCAGATGAAGGGCGGCGGC
ACCCTCGTGACCTTCGTGGTGGAGGGCGGCAAGGAGGGCGCCTTCCGTTTCGCCAACGCCCTCGGGCTGATCCGCATCTC
CAACAACCTCGGCGATGCCAAGAGCCTGATCACCCATCCGGCCACCACCACCCACCAGCGCTTCACGCCGGAGGCCCGCG
CCGAGATGGGGATTTCCGACGGCCTGCTTCGCCTGTCCGTGGGGCTCGAGCATCCCGACGACCTGATCGCCGATCTCGCC
ACCGCCGCCGGAGCCTTGTAA

Upstream 100 bases:

>100_bases
AAGGCCGGGGACGAACAGACGGGCAAGCCGCGCGCGCCGCACACCGGCCACCGCGGCGGCGAACCGCGCCTGATCCCCTC
GGCCTCCCCCGGAGACCGTC

Downstream 100 bases:

>100_bases
CGGCTCCATTCCGGCGACGTGACGGAGCGGACTGTTGCGCCCGGACGGCAACGCGGGTTCACTGTGGCCCGCGGGGGCAT
TTTCTCCCGCGGTGAGGGGA

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSVAQKPAPKFIPKDPLTLHPETRLVHGGTTRSPYGETSEALFLTQGFVYDNAESCEARFKGEDPGFVYSRYGNPTTAMF
EERMALLEGAEAARATASGMAAVNAALLCQLSAGDHVVAARALFGSCRWILEDFLPRFGIETTLVDGTDLAAWQAAARPS
TKVFFLESPTNPTLELIDIAAVAQIAHAAGARLVVDNVFATPLLQHPFELGADIVVYSTTKHVDGQGRCLGGVILSSQQF
VTDHLQTFLRQTGPSISPFNAWIMLKGLETLALRVERMQASAGRIADALAGLPKIETVLYPGRADHPQAELARRQMKGGG
TLVTFVVEGGKEGAFRFANALGLIRISNNLGDAKSLITHPATTTHQRFTPEARAEMGISDGLLRLSVGLEHPDDLIADLA
TAAGAL

Sequences:

>Translated_406_residues
MSVAQKPAPKFIPKDPLTLHPETRLVHGGTTRSPYGETSEALFLTQGFVYDNAESCEARFKGEDPGFVYSRYGNPTTAMF
EERMALLEGAEAARATASGMAAVNAALLCQLSAGDHVVAARALFGSCRWILEDFLPRFGIETTLVDGTDLAAWQAAARPS
TKVFFLESPTNPTLELIDIAAVAQIAHAAGARLVVDNVFATPLLQHPFELGADIVVYSTTKHVDGQGRCLGGVILSSQQF
VTDHLQTFLRQTGPSISPFNAWIMLKGLETLALRVERMQASAGRIADALAGLPKIETVLYPGRADHPQAELARRQMKGGG
TLVTFVVEGGKEGAFRFANALGLIRISNNLGDAKSLITHPATTTHQRFTPEARAEMGISDGLLRLSVGLEHPDDLIADLA
TAAGAL
>Mature_405_residues
SVAQKPAPKFIPKDPLTLHPETRLVHGGTTRSPYGETSEALFLTQGFVYDNAESCEARFKGEDPGFVYSRYGNPTTAMFE
ERMALLEGAEAARATASGMAAVNAALLCQLSAGDHVVAARALFGSCRWILEDFLPRFGIETTLVDGTDLAAWQAAARPST
KVFFLESPTNPTLELIDIAAVAQIAHAAGARLVVDNVFATPLLQHPFELGADIVVYSTTKHVDGQGRCLGGVILSSQQFV
TDHLQTFLRQTGPSISPFNAWIMLKGLETLALRVERMQASAGRIADALAGLPKIETVLYPGRADHPQAELARRQMKGGGT
LVTFVVEGGKEGAFRFANALGLIRISNNLGDAKSLITHPATTTHQRFTPEARAEMGISDGLLRLSVGLEHPDDLIADLAT
AAGAL

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=36.890243902439, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=33.8414634146341, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=30.5810397553517, Blast_Score=155, Evalue=8e-38,
Organism=Escherichia coli, GI1790375, Length=331, Percent_Identity=36.8580060422961, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1789383, Length=387, Percent_Identity=25.5813953488372, Blast_Score=124, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17560056, Length=343, Percent_Identity=36.4431486880466, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=36.4457831325301, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI71980708, Length=364, Percent_Identity=34.8901098901099, Blast_Score=207, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=34.625322997416, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6323333, Length=403, Percent_Identity=33.4987593052109, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=26.8472906403941, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=272, Percent_Identity=30.1470588235294, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=399, Percent_Identity=26.8170426065163, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922554, Length=344, Percent_Identity=37.5, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43418; Mature: 43287

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAQKPAPKFIPKDPLTLHPETRLVHGGTTRSPYGETSEALFLTQGFVYDNAESCEARF
CCCCCCCCCCCCCCCCCEECCCCEEEECCCCCCCCCCCCCEEEEEECEEECCCHHHHHCC
KGEDPGFVYSRYGNPTTAMFEERMALLEGAEAARATASGMAAVNAALLCQLSAGDHVVAA
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCHHHH
RALFGSCRWILEDFLPRFGIETTLVDGTDLAAWQAAARPSTKVFFLESPTNPTLELIDIA
HHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHH
AVAQIAHAAGARLVVDNVFATPLLQHPFELGADIVVYSTTKHVDGQGRCLGGVILSSQQF
HHHHHHHHCCCEEEEHHHHHHHHHHCHHHCCCCEEEEECCCCCCCCCCEEEEEEECCHHH
VTDHLQTFLRQTGPSISPFNAWIMLKGLETLALRVERMQASAGRIADALAGLPKIETVLY
HHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
PGRADHPQAELARRQMKGGGTLVTFVVEGGKEGAFRFANALGLIRISNNLGDAKSLITHP
CCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHEEEEEEECCCCCHHHHHCCC
ATTTHQRFTPEARAEMGISDGLLRLSVGLEHPDDLIADLATAAGAL
CCCCCCCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVAQKPAPKFIPKDPLTLHPETRLVHGGTTRSPYGETSEALFLTQGFVYDNAESCEARF
CCCCCCCCCCCCCCCCEECCCCEEEECCCCCCCCCCCCCEEEEEECEEECCCHHHHHCC
KGEDPGFVYSRYGNPTTAMFEERMALLEGAEAARATASGMAAVNAALLCQLSAGDHVVAA
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCHHHH
RALFGSCRWILEDFLPRFGIETTLVDGTDLAAWQAAARPSTKVFFLESPTNPTLELIDIA
HHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHH
AVAQIAHAAGARLVVDNVFATPLLQHPFELGADIVVYSTTKHVDGQGRCLGGVILSSQQF
HHHHHHHHCCCEEEEHHHHHHHHHHCHHHCCCCEEEEECCCCCCCCCCEEEEEEECCHHH
VTDHLQTFLRQTGPSISPFNAWIMLKGLETLALRVERMQASAGRIADALAGLPKIETVLY
HHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
PGRADHPQAELARRQMKGGGTLVTFVVEGGKEGAFRFANALGLIRISNNLGDAKSLITHP
CCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHEEEEEEECCCCCHHHHHCCC
ATTTHQRFTPEARAEMGISDGLLRLSVGLEHPDDLIADLATAAGAL
CCCCCCCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]