Click here to switch to the map view.

The map label for this gene is ligT [C]

Identifier: 154244365

GI number: 154244365

Start: 438134

End: 438664

Strand: Reverse

Name: ligT [C]

Synonym: Xaut_0408

Alternate gene names: 154244365

Gene position: 438664-438134 (Counterclockwise)

Preceding gene: 154244366

Following gene: 154244364

Centisome position: 8.26

GC content: 68.55

Gene sequence:

>531_bases
ATGCCGCGACTGTTCACCGCCATCGAGATCCCCCCCGAGGTCGGGCTGGGCCTGTCGCTGCTGCGCGGCGGACTTTCCGG
TGCCCGCTGGATTGATCCCGAGAACTATCACGTCACCCTGCGCTTCGTCGGCGACGTGGATGACGCCACAGCCCGCGATA
TCATGCTGATGCTGGGCCAGGTGCGCCGGCCCGCCTTCGACCTGACGCTGGACGGCATCGACCAGTTCGGCGGCCACAAG
CCCCGCGCGGTGTTCGCGGCGGTGAAGGCCAATGCGGCCCTCATGGAGTTGCAGGCCGAGCAGGAGCGCATCATGCAGCG
CCTCGGCCTCGGCGCGGAACGCAATTTCAAGCCCCACGTCACCCTGGCCCGGCTGCGGGACGCCTCCTCGCGGCAGGTGG
CTGATTATCTCGCCATGCGCGGCCCCTATCGCAGCCCGACGTTCCACGTGCCGCGCTTCGTGCTGTTCTCCTCGCGCGAT
TCGGTGGGCGGCGGCCCCTATTTGGTGGAGGCAGCCTACCCGCTGCTCTGA

Upstream 100 bases:

>100_bases
TTCACTTGGGGCATCGTCGCTCCACCGGGCGGTCCCGCCCCGGCGCCGGCGCTGTCTGAGCCGACCCGTGGCCACGGGCC
GGCCGATCGCGGAGGGTTCC

Downstream 100 bases:

>100_bases
CGCAGGGTCGCCCAGAGGCCTTGCGGGCGGGCGAAGCCTTCCCAAATGTTGGGCATGACCAGCACCATTCTGGATGACGA
CGGCCTGTCCCCCGATGAAC

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MPRLFTAIEIPPEVGLGLSLLRGGLSGARWIDPENYHVTLRFVGDVDDATARDIMLMLGQVRRPAFDLTLDGIDQFGGHK
PRAVFAAVKANAALMELQAEQERIMQRLGLGAERNFKPHVTLARLRDASSRQVADYLAMRGPYRSPTFHVPRFVLFSSRD
SVGGGPYLVEAAYPLL

Sequences:

>Translated_176_residues
MPRLFTAIEIPPEVGLGLSLLRGGLSGARWIDPENYHVTLRFVGDVDDATARDIMLMLGQVRRPAFDLTLDGIDQFGGHK
PRAVFAAVKANAALMELQAEQERIMQRLGLGAERNFKPHVTLARLRDASSRQVADYLAMRGPYRSPTFHVPRFVLFSSRD
SVGGGPYLVEAAYPLL
>Mature_175_residues
PRLFTAIEIPPEVGLGLSLLRGGLSGARWIDPENYHVTLRFVGDVDDATARDIMLMLGQVRRPAFDLTLDGIDQFGGHKP
RAVFAAVKANAALMELQAEQERIMQRLGLGAERNFKPHVTLARLRDASSRQVADYLAMRGPYRSPTFHVPRFVLFSSRDS
VGGGPYLVEAAYPLL

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19476; Mature: 19345

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFTAIEIPPEVGLGLSLLRGGLSGARWIDPENYHVTLRFVGDVDDATARDIMLMLGQ
CCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
VRRPAFDLTLDGIDQFGGHKPRAVFAAVKANAALMELQAEQERIMQRLGLGAERNFKPHV
HCCCEEEEEECCHHHHCCCCCHHEEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
TLARLRDASSRQVADYLAMRGPYRSPTFHVPRFVLFSSRDSVGGGPYLVEAAYPLL
EHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
PRLFTAIEIPPEVGLGLSLLRGGLSGARWIDPENYHVTLRFVGDVDDATARDIMLMLGQ
CCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
VRRPAFDLTLDGIDQFGGHKPRAVFAAVKANAALMELQAEQERIMQRLGLGAERNFKPHV
HCCCEEEEEECCHHHHCCCCCHHEEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
TLARLRDASSRQVADYLAMRGPYRSPTFHVPRFVLFSSRDSVGGGPYLVEAAYPLL
EHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]