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The map label for this gene is fusA [H]

Identifier: 153950467

GI number: 153950467

Start: 4398384

End: 4400492

Strand: Reverse

Name: fusA [H]

Synonym: YpsIP31758_3919

Alternate gene names: 153950467

Gene position: 4400492-4398384 (Counterclockwise)

Preceding gene: 153949585

Following gene: 153949761

Centisome position: 93.17

GC content: 48.13

Gene sequence:

>2109_bases
ATGGCTCGTAAAACACCCATTGAGCGCTATCGTAATATCGGTATCAGCGCTCACATCGACGCCGGTAAGACAACCACTAC
CGAACGTATCCTGTTTTACACCGGTGTAAATCACAAAATCGGTGAAGTTCATGACGGCGCAGCCACCATGGACTGGATGG
AACAGGAGCAGGAGCGTGGTATTACCATTACTTCTGCAGCTACTACCTGCTTCTGGTCTGGTATGGCTAAACAGTTCGAA
CCACATCACGTCAATATCATTGACACCCCTGGGCACGTTGACTTCACTATCGAAGTAGAGCGTTCCATGCGTGTTCTTGA
CGGCGCGGTAATGGTTTACTGTGCAGTTGGTGGTGTTCAGCCACAGTCTGAAACCGTATGGCGTCAGGCTAATAAATATA
AAGTTCCACGTATTGCGTTCGTTAACAAAATGGACCGTATGGGTGCGAACTTCCTGCGCGTAGTTGGTCAACTGAAATCT
CGCCTTGGTGCGAACCCAGTTCCACTGCAGTTGGCAATTGGCGCAGAAGAAAAATTCACCGGTATTATCGATCTGGTGAA
AATGAAAGCGATCAACTGGAACGAAGCTGATCAGGGCGTGACCTTCGAATATGAAGAAATCCCTGCTGATATGGCTGAAC
TGGCTGCTGAATGGCACCAGAATCTGGTTGAATCTGCGGCAGAAGCGTCTGACGAGCTGATGGACAAATACTTGGGTGGC
GAAGAGCTGACCGAAGAAGAAATCAAGAAAGCTTTACGTCAACGTGTTCTGAAAAGCGAAATTATTCTTGTTACCTGTGG
TTCTGCGTTTAAAAACAAAGGCGTACAGGCAATGCTGGATGCGGTTATTGAGTACCTGCCTGCACCAACTGACGTTGAAT
CAATCAACGGCATCTTGGATGATGGCAAAGATACTCCGGCTGTTCGTCATTCTGACGACAAAGAGCCGTTCTCTGCTCTG
GCGTTCAAAATCGCTACCGACCCATTCGTGGGTAACCTGACGTTCTTCCGCGTGTACTCTGGTATTGTTAATTCCGGTGA
TACCGTTCTGAACTCAGTGAAATCGCAACGTGAACGCTTAGGTCGTATCGTACAGATGCACGCTAACAAGCGTGAAGAGA
TCAAAGAAGTTCACGCCGGTGATATCGCAGCCGCTATCGGTCTGAAAGATGTGACTACGGGTGACACTTTGTGTGACCCG
AATAATCCGATCATCTTGGAACGTATGGAGTTCCCAGAGCCGGTAATCTCTGTTGCTGTTGAACCAAAAACCAAAGCTGA
CCAAGAAAAAATGGGTATGGCTCTGGGGCGTTTGGCGAAAGAAGATCCATCATTCCGCGTTTGGACTGACGAAGAATCTG
GTCAGACTATCATCGCTGGTATGGGTGAGTTGCATTTGGATATCCTGGTTGACCGTATGCGCCGCGAATTTAACGTGGAA
GCAAACGTCGGTAAACCTCAGGTTGCGTACCGTGAAACTATCCGCGAAACCGTTAAGGATGTGGAAGGTAAGCACGCTAA
GCAGTCAGGCGGTCGTGGTCAGTATGGTCATGTTGTTATCGACATGTCTCCATTGCCACCGGGTGGTGTTGGGTATGAAT
TCGTCAACGAAATCGTTGGTGGTTCTATTCCTAAAGAATTCATTCCGGCCGTTGATAAAGGTATTCAAGAACAGCTGAAA
TCTGGCCCTCTGGCAGGTTACCCAGTTGTTGACGTTAAAGTGCGTCTGCACTACGGTTCTTACCATGACGTTGACTCCTC
AGAATTGGCATTTAAATTAGCTGGTTCTATCGCCTTTAAAGAAGGTTTCAAACGAGCTAAACCAGTTCTGCTTGAGCCAA
TCATGAAGGTTGAAGTCGAAACCCCTGAAGATTACATGGGTGACGTAATGGGCGACCTGAACCGTCGTCGCGGTATCATC
GAAGGTATGGAAGATACTGCTACCGGTAAAACTGTTCGCGTCAAGGTTCCGTTGTCTGAAATGTTCGGTTATGCTACTGA
CCTGCGTTCTCAGACTCAGGGCCGTGCTTCTTACTCCATGGAATTCTTGGAGTATGCTGAAGCACCGAGTAACGTCGCTA
AAGCCGTTATCGAAGCCCGTGGCAAATAA

Upstream 100 bases:

>100_bases
CTGGTAATATCCCGCAGTAGTCATGCTAACCATGCGGGCGCTTCAAGAAGCCAACCCGCATGGGTTAACCGAATGAACGC
CCTAGGAATAGAGGAATCAA

Downstream 100 bases:

>100_bases
GCTTTCGGGTTTAAAACAATGATCCAGTGCTCTCTCATTAGTGGGAGAGCACAAGAGTAAGGAATATAGTCGTGTCTAAA
GAAAAATTTGAACGTACCAA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTCFWSGMAKQFE
PHHVNIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLRVVGQLKS
RLGANPVPLQLAIGAEEKFTGIIDLVKMKAINWNEADQGVTFEYEEIPADMAELAAEWHQNLVESAAEASDELMDKYLGG
EELTEEEIKKALRQRVLKSEIILVTCGSAFKNKGVQAMLDAVIEYLPAPTDVESINGILDDGKDTPAVRHSDDKEPFSAL
AFKIATDPFVGNLTFFRVYSGIVNSGDTVLNSVKSQRERLGRIVQMHANKREEIKEVHAGDIAAAIGLKDVTTGDTLCDP
NNPIILERMEFPEPVISVAVEPKTKADQEKMGMALGRLAKEDPSFRVWTDEESGQTIIAGMGELHLDILVDRMRREFNVE
ANVGKPQVAYRETIRETVKDVEGKHAKQSGGRGQYGHVVIDMSPLPPGGVGYEFVNEIVGGSIPKEFIPAVDKGIQEQLK
SGPLAGYPVVDVKVRLHYGSYHDVDSSELAFKLAGSIAFKEGFKRAKPVLLEPIMKVEVETPEDYMGDVMGDLNRRRGII
EGMEDTATGKTVRVKVPLSEMFGYATDLRSQTQGRASYSMEFLEYAEAPSNVAKAVIEARGK

Sequences:

>Translated_702_residues
MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTCFWSGMAKQFE
PHHVNIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLRVVGQLKS
RLGANPVPLQLAIGAEEKFTGIIDLVKMKAINWNEADQGVTFEYEEIPADMAELAAEWHQNLVESAAEASDELMDKYLGG
EELTEEEIKKALRQRVLKSEIILVTCGSAFKNKGVQAMLDAVIEYLPAPTDVESINGILDDGKDTPAVRHSDDKEPFSAL
AFKIATDPFVGNLTFFRVYSGIVNSGDTVLNSVKSQRERLGRIVQMHANKREEIKEVHAGDIAAAIGLKDVTTGDTLCDP
NNPIILERMEFPEPVISVAVEPKTKADQEKMGMALGRLAKEDPSFRVWTDEESGQTIIAGMGELHLDILVDRMRREFNVE
ANVGKPQVAYRETIRETVKDVEGKHAKQSGGRGQYGHVVIDMSPLPPGGVGYEFVNEIVGGSIPKEFIPAVDKGIQEQLK
SGPLAGYPVVDVKVRLHYGSYHDVDSSELAFKLAGSIAFKEGFKRAKPVLLEPIMKVEVETPEDYMGDVMGDLNRRRGII
EGMEDTATGKTVRVKVPLSEMFGYATDLRSQTQGRASYSMEFLEYAEAPSNVAKAVIEARGK
>Mature_701_residues
ARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTCFWSGMAKQFEP
HHVNIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLRVVGQLKSR
LGANPVPLQLAIGAEEKFTGIIDLVKMKAINWNEADQGVTFEYEEIPADMAELAAEWHQNLVESAAEASDELMDKYLGGE
ELTEEEIKKALRQRVLKSEIILVTCGSAFKNKGVQAMLDAVIEYLPAPTDVESINGILDDGKDTPAVRHSDDKEPFSALA
FKIATDPFVGNLTFFRVYSGIVNSGDTVLNSVKSQRERLGRIVQMHANKREEIKEVHAGDIAAAIGLKDVTTGDTLCDPN
NPIILERMEFPEPVISVAVEPKTKADQEKMGMALGRLAKEDPSFRVWTDEESGQTIIAGMGELHLDILVDRMRREFNVEA
NVGKPQVAYRETIRETVKDVEGKHAKQSGGRGQYGHVVIDMSPLPPGGVGYEFVNEIVGGSIPKEFIPAVDKGIQEQLKS
GPLAGYPVVDVKVRLHYGSYHDVDSSELAFKLAGSIAFKEGFKRAKPVLLEPIMKVEVETPEDYMGDVMGDLNRRRGIIE
GMEDTATGKTVRVKVPLSEMFGYATDLRSQTQGRASYSMEFLEYAEAPSNVAKAVIEARGK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=712, Percent_Identity=44.8033707865169, Blast_Score=587, Evalue=1e-167,
Organism=Homo sapiens, GI19923640, Length=723, Percent_Identity=38.4508990318119, Blast_Score=451, Evalue=1e-126,
Organism=Homo sapiens, GI25306283, Length=462, Percent_Identity=40.4761904761905, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI25306287, Length=297, Percent_Identity=47.8114478114478, Blast_Score=253, Evalue=5e-67,
Organism=Homo sapiens, GI4503483, Length=488, Percent_Identity=26.4344262295082, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157426893, Length=154, Percent_Identity=35.7142857142857, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=38.6861313868613, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310132016, Length=120, Percent_Identity=39.1666666666667, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI310110807, Length=120, Percent_Identity=39.1666666666667, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI310123363, Length=120, Percent_Identity=39.1666666666667, Blast_Score=81, Evalue=4e-15,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=86.9318181818182, Blast_Score=1276, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=498, Percent_Identity=27.5100401606426, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI48994988, Length=152, Percent_Identity=39.4736842105263, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=155, Percent_Identity=35.4838709677419, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=690, Percent_Identity=43.6231884057971, Blast_Score=546, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17556745, Length=735, Percent_Identity=28.7074829931973, Blast_Score=298, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI17506493, Length=489, Percent_Identity=26.9938650306748, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=163, Percent_Identity=37.4233128834356, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=148, Percent_Identity=33.7837837837838, Blast_Score=84, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71988811, Length=147, Percent_Identity=34.6938775510204, Blast_Score=83, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=698, Percent_Identity=43.9828080229226, Blast_Score=582, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6322359, Length=797, Percent_Identity=30.99121706399, Blast_Score=372, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324707, Length=482, Percent_Identity=26.9709543568465, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=482, Percent_Identity=26.9709543568465, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323320, Length=180, Percent_Identity=34.4444444444444, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=40, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24582462, Length=703, Percent_Identity=45.945945945946, Blast_Score=618, Evalue=1e-177,
Organism=Drosophila melanogaster, GI221458488, Length=742, Percent_Identity=33.0188679245283, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24585709, Length=495, Percent_Identity=26.8686868686869, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585711, Length=495, Percent_Identity=26.8686868686869, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24585713, Length=495, Percent_Identity=26.8686868686869, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI78706572, Length=186, Percent_Identity=34.9462365591398, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28574573, Length=143, Percent_Identity=35.6643356643357, Blast_Score=84, Evalue=5e-16,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77538; Mature: 77407

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERG
CCCCCHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTCFWSGMAKQFEPHHVNIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQ
EEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECHHHHHHHHHHEEEEECCCCC
PQSETVWRQANKYKVPRIAFVNKMDRMGANFLRVVGQLKSRLGANPVPLQLAIGAEEKFT
CCHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
GIIDLVKMKAINWNEADQGVTFEYEEIPADMAELAAEWHQNLVESAAEASDELMDKYLGG
HHHHHHHHHCCCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EELTEEEIKKALRQRVLKSEIILVTCGSAFKNKGVQAMLDAVIEYLPAPTDVESINGILD
CCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHC
DGKDTPAVRHSDDKEPFSALAFKIATDPFVGNLTFFRVYSGIVNSGDTVLNSVKSQRERL
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GRIVQMHANKREEIKEVHAGDIAAAIGLKDVTTGDTLCDPNNPIILERMEFPEPVISVAV
HHHHHHHCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHEEEE
EPKTKADQEKMGMALGRLAKEDPSFRVWTDEESGQTIIAGMGELHLDILVDRMRREFNVE
CCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCCCC
ANVGKPQVAYRETIRETVKDVEGKHAKQSGGRGQYGHVVIDMSPLPPGGVGYEFVNEIVG
CCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHC
GSIPKEFIPAVDKGIQEQLKSGPLAGYPVVDVKVRLHYGSYHDVDSSELAFKLAGSIAFK
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
EGFKRAKPVLLEPIMKVEVETPEDYMGDVMGDLNRRRGIIEGMEDTATGKTVRVKVPLSE
HHHHHCCCHHHCCHHEEEECCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEEECHHH
MFGYATDLRSQTQGRASYSMEFLEYAEAPSNVAKAVIEARGK
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERG
CCCCHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTCFWSGMAKQFEPHHVNIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQ
EEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECHHHHHHHHHHEEEEECCCCC
PQSETVWRQANKYKVPRIAFVNKMDRMGANFLRVVGQLKSRLGANPVPLQLAIGAEEKFT
CCHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
GIIDLVKMKAINWNEADQGVTFEYEEIPADMAELAAEWHQNLVESAAEASDELMDKYLGG
HHHHHHHHHCCCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EELTEEEIKKALRQRVLKSEIILVTCGSAFKNKGVQAMLDAVIEYLPAPTDVESINGILD
CCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHC
DGKDTPAVRHSDDKEPFSALAFKIATDPFVGNLTFFRVYSGIVNSGDTVLNSVKSQRERL
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GRIVQMHANKREEIKEVHAGDIAAAIGLKDVTTGDTLCDPNNPIILERMEFPEPVISVAV
HHHHHHHCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHEEEE
EPKTKADQEKMGMALGRLAKEDPSFRVWTDEESGQTIIAGMGELHLDILVDRMRREFNVE
CCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCCCC
ANVGKPQVAYRETIRETVKDVEGKHAKQSGGRGQYGHVVIDMSPLPPGGVGYEFVNEIVG
CCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHC
GSIPKEFIPAVDKGIQEQLKSGPLAGYPVVDVKVRLHYGSYHDVDSSELAFKLAGSIAFK
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
EGFKRAKPVLLEPIMKVEVETPEDYMGDVMGDLNRRRGIIEGMEDTATGKTVRVKVPLSE
HHHHHCCCHHHCCHHEEEECCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEEECHHH
MFGYATDLRSQTQGRASYSMEFLEYAEAPSNVAKAVIEARGK
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA