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The map label for this gene is ddl [H]

Identifier: 153952610

GI number: 153952610

Start: 1064216

End: 1065256

Strand: Direct

Name: ddl [H]

Synonym: JJD26997_1212

Alternate gene names: 153952610

Gene position: 1064216-1065256 (Clockwise)

Preceding gene: 153952036

Following gene: 153951827

Centisome position: 57.68

GC content: 29.11

Gene sequence:

>1041_bases
ATGAAATTTGCAATACTTTTTGGCGGAAATTCTTATGAACATGAGATCAGCATAGTAAGCGCTGTTGCACTTAAAAAAGT
CATTAATCAAAATTTAGAATTTATTTTTTGTGATGAAGAAAGAAGATTTTATCATATCCCAAGTGAAAAAATGAATTCTA
AAACTTTTAGCACTAAAGCTTATAAAAAAGAAAAAGAACTTTTCATACAACAAGGAGGTTTTTTTAGTAAAGGTTTTTTA
AAAGAAAATAAACTAGAGTGCGAGTGCGTGATCAATCTTATCCATGGTGGAGATGGAGAAGATGGAAAAATCGCGGCTTT
ATTTGAGTTTTATTCTATTAAATTTATAGGACCTAGACTTGAAGCTAGCGTATTATCTTTTAACAAGGAACTTACTAAAC
TTTATGCAAAAAGCGTTGGAGTAAAAACACTTGATTATACCATGCTAAGAAAAGGGCAAAACTCTAAAGAAAAGCTTCGT
TTTCCTTGTATTATCAAACCTGCAAGACTTGGAAGTAGCATAGGCATTAGCATAGTAAAAGATGAAAAAGATTTAGAATA
CGCCAAAGATGTAGGTTTTGAATTTGACAATGATCTTGTAGTAGAAGAATTTAAAAACAATATCAAAGAGTATAATCTTG
CAGGATGTGTGATCAATGATAAGTTTGTTTTTTCCATCATAGAAGAGCCAAAGAAAAAAGAATTTTTAGACTTTGAACAA
AAATATCTTAGCTTTTCAGGACACAATGAACTTATAGAAGCAAATTTAAGCGAAGAATTAAAAGAAAAACTCAAAGATAG
CTTTAAAAAAATTTACAATCCTTTATTTAAAGGTGCTTTAATACGCTGCGATTTCTTTATACTAGATAATGAAGTATATT
TAAATGAAATCAATCCAAATCCTGGTTCTTTAGCTAATTATCTTTTTAAAGATTTTAGTACCACTTTAAACACTTTAGCT
GATCAAATTCCGCTTGAAAAAATGATAAAAATCAACTACAACTTCCTTCACTCCATTAACGGACAAAAAGGAAAATTATA
A

Upstream 100 bases:

>100_bases
TAAACAAGAAAAAATCATAAGTGTTTTAGCAAGTGCTCAGGCAACAGGCACAAGCGAACTCATCAAAGAAGCTCTTAAAA
AACTAAGATAAAGGAATAAA

Downstream 100 bases:

>100_bases
GTTTTTAAGTCAAAATACGCTAAATTATACATAAAATTTAGCGTATTTTAGGAAGAAAAATGCTCTCTTTTAAACAAGAT
GAAATTTACACCGCCACCGA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKFAILFGGNSYEHEISIVSAVALKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKAYKKEKELFIQQGGFFSKGFL
KENKLECECVINLIHGGDGEDGKIAALFEFYSIKFIGPRLEASVLSFNKELTKLYAKSVGVKTLDYTMLRKGQNSKEKLR
FPCIIKPARLGSSIGISIVKDEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCVINDKFVFSIIEEPKKKEFLDFEQ
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEVYLNEINPNPGSLANYLFKDFSTTLNTLA
DQIPLEKMIKINYNFLHSINGQKGKL

Sequences:

>Translated_346_residues
MKFAILFGGNSYEHEISIVSAVALKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKAYKKEKELFIQQGGFFSKGFL
KENKLECECVINLIHGGDGEDGKIAALFEFYSIKFIGPRLEASVLSFNKELTKLYAKSVGVKTLDYTMLRKGQNSKEKLR
FPCIIKPARLGSSIGISIVKDEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCVINDKFVFSIIEEPKKKEFLDFEQ
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEVYLNEINPNPGSLANYLFKDFSTTLNTLA
DQIPLEKMIKINYNFLHSINGQKGKL
>Mature_346_residues
MKFAILFGGNSYEHEISIVSAVALKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKAYKKEKELFIQQGGFFSKGFL
KENKLECECVINLIHGGDGEDGKIAALFEFYSIKFIGPRLEASVLSFNKELTKLYAKSVGVKTLDYTMLRKGQNSKEKLR
FPCIIKPARLGSSIGISIVKDEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCVINDKFVFSIIEEPKKKEFLDFEQ
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEVYLNEINPNPGSLANYLFKDFSTTLNTLA
DQIPLEKMIKINYNFLHSINGQKGKL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=330, Percent_Identity=25.4545454545455, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786280, Length=319, Percent_Identity=26.9592476489028, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39776; Mature: 39776

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAILFGGNSYEHEISIVSAVALKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHCCCCCHHHH
YKKEKELFIQQGGFFSKGFLKENKLECECVINLIHGGDGEDGKIAALFEFYSIKFIGPRL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEEEEECCCH
EASVLSFNKELTKLYAKSVGVKTLDYTMLRKGQNSKEKLRFPCIIKPARLGSSIGISIVK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCCCEEEEHHHCCCCCCEEEEE
DEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCVINDKFVFSIIEEPKKKEFLDFEQ
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCHHHHHHHHH
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEVYLNEINPN
HHHCCCCCCHHEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECEEEEEECCCC
PGSLANYLFKDFSTTLNTLADQIPLEKMIKINYNFLHSINGQKGKL
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECHHHEECCCCCCCCC
>Mature Secondary Structure
MKFAILFGGNSYEHEISIVSAVALKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHCCCCCHHHH
YKKEKELFIQQGGFFSKGFLKENKLECECVINLIHGGDGEDGKIAALFEFYSIKFIGPRL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEEEEECCCH
EASVLSFNKELTKLYAKSVGVKTLDYTMLRKGQNSKEKLRFPCIIKPARLGSSIGISIVK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCCCEEEEHHHCCCCCCEEEEE
DEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCVINDKFVFSIIEEPKKKEFLDFEQ
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCHHHHHHHHH
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEVYLNEINPN
HHHCCCCCCHHEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECEEEEEECCCC
PGSLANYLFKDFSTTLNTLADQIPLEKMIKINYNFLHSINGQKGKL
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA