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The map label for this gene is queA [H]

Identifier: 153951843

GI number: 153951843

Start: 963564

End: 964595

Strand: Direct

Name: queA [H]

Synonym: JJD26997_1092

Alternate gene names: 153951843

Gene position: 963564-964595 (Clockwise)

Preceding gene: 153951649

Following gene: 153951818

Centisome position: 52.22

GC content: 30.81

Gene sequence:

>1032_bases
GTGATAAATAAAGATTTACTTCTTTCAAGCTATGATTACACTCTAGCTAATGAACTCATAGCCAACTATCCTACAAATCC
AAAAGAAGATGCTAGGCTTTTGGTTTTTGATCGCAAAAACAAAGAAATTTTTCACACTACTTTTAAAAATTTACAAGACT
TTTTACCCAATTGTGCGATTTTTTTCAATGATACCAAAGTTATTAAAGCAAGAATTTATGGCAATAAAGCAAGCGGAGGA
AAAATAGAGCTTTTCTTACATCAACCTTTTTTAAATTCTCACAATCCGCTTTTTCTAGCTCAAATTAAAGGGCGTGTTAA
AAAAGATGAAATCTTATATTTTAAAGAGGATTTAAAAGCATACATTGTAGAACTTTTAAATGAGGGTCTCCGAAAAGTTC
AATTTTTTCAAAATGATAAAACCTTAGACACCTCAAATCTTTATAACCTGCTTGACAAAATAGGACATATACCCTTGCCT
CCTTATATTAAAAGAGAGGATGAAAAAAGTGATTTGAAAGATTATCAAAGTATCTTTGCAAAAAATTTAGGCGCAGTAGC
TGCTCCGACTGCAAGTTTACATTTTAGTGAGATTATGCTTGAAAATTTAAGAAAAAAACACGAAATTTATCATTTAACCT
TGCATGTAGGAGCTGGAACCTTTAAAGGCGTAGAATGTGAAAATATACAAGAGCATAAAATGCATAGTGAATTTTTTAAT
ATTCCTAAGAGAGCATGTGAAATTATAGATTCTAAACAAGCTATTTTAGGCGTAGGAACAACGGTAACAAGAACCATAGA
ATATTATGCAAGAACTAAAACAAAAAATGGTTTTTGTGATCTTTTTTTACACCCGCAAAATCCACCTATAAGACAAAATC
ATCTTTTGACCAATTTTCATTTACCAAAATCTACTCTTATCATGCTAGTTTCAGCTTTTATCGGTAGAGAACAGTGTTTA
AAACTTTATGAACTCGCCATAAAAGAAAAATATCGCTTTTACTCTTATGGCGATGCGATGCTGATTTTATAA

Upstream 100 bases:

>100_bases
GTTTTATCTCAATTTTTAATGGCAGGACCACTTTGCGGACTTTATGGACTTTCTATTTTAATTGTTCAAAAGGTAAATCC
TACCCCAAAAGATAAAGAAA

Downstream 100 bases:

>100_bases
ATGTCAGTTCCCCGCCAAAAAATACCACCTTTCTAACCTTAACTCTCTTTTTCAACTCTCTAGCTTTTTAGATTTAAGAA
CCTTTCTAAATTTAATTTTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MINKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAIFFNDTKVIKARIYGNKASGG
KIELFLHQPFLNSHNPLFLAQIKGRVKKDEILYFKEDLKAYIVELLNEGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLP
PYIKREDEKSDLKDYQSIFAKNLGAVAAPTASLHFSEIMLENLRKKHEIYHLTLHVGAGTFKGVECENIQEHKMHSEFFN
IPKRACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFHLPKSTLIMLVSAFIGREQCL
KLYELAIKEKYRFYSYGDAMLIL

Sequences:

>Translated_343_residues
MINKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAIFFNDTKVIKARIYGNKASGG
KIELFLHQPFLNSHNPLFLAQIKGRVKKDEILYFKEDLKAYIVELLNEGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLP
PYIKREDEKSDLKDYQSIFAKNLGAVAAPTASLHFSEIMLENLRKKHEIYHLTLHVGAGTFKGVECENIQEHKMHSEFFN
IPKRACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFHLPKSTLIMLVSAFIGREQCL
KLYELAIKEKYRFYSYGDAMLIL
>Mature_343_residues
MINKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAIFFNDTKVIKARIYGNKASGG
KIELFLHQPFLNSHNPLFLAQIKGRVKKDEILYFKEDLKAYIVELLNEGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLP
PYIKREDEKSDLKDYQSIFAKNLGAVAAPTASLHFSEIMLENLRKKHEIYHLTLHVGAGTFKGVECENIQEHKMHSEFFN
IPKRACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFHLPKSTLIMLVSAFIGREQCL
KLYELAIKEKYRFYSYGDAMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=350, Percent_Identity=38.8571428571429, Blast_Score=237, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39714; Mature: 39714

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAI
CCCHHHEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEE
FFNDTKVIKARIYGNKASGGKIELFLHQPFLNSHNPLFLAQIKGRVKKDEILYFKEDLKA
EECCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEEHHHHHH
YIVELLNEGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLPPYIKREDEKSDLKDYQSIFA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
KNLGAVAAPTASLHFSEIMLENLRKKHEIYHLTLHVGAGTFKGVECENIQEHKMHSEFFN
HCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHC
IPKRACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFH
CHHHHHHHHCCCHHEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEC
LPKSTLIMLVSAFIGREQCLKLYELAIKEKYRFYSYGDAMLIL
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure
MINKDLLLSSYDYTLANELIANYPTNPKEDARLLVFDRKNKEIFHTTFKNLQDFLPNCAI
CCCHHHEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEE
FFNDTKVIKARIYGNKASGGKIELFLHQPFLNSHNPLFLAQIKGRVKKDEILYFKEDLKA
EECCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEEHHHHHH
YIVELLNEGLRKVQFFQNDKTLDTSNLYNLLDKIGHIPLPPYIKREDEKSDLKDYQSIFA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
KNLGAVAAPTASLHFSEIMLENLRKKHEIYHLTLHVGAGTFKGVECENIQEHKMHSEFFN
HCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHC
IPKRACEIIDSKQAILGVGTTVTRTIEYYARTKTKNGFCDLFLHPQNPPIRQNHLLTNFH
CHHHHHHHHCCCHHEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEC
LPKSTLIMLVSAFIGREQCLKLYELAIKEKYRFYSYGDAMLIL
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA