| Definition | Clostridium botulinum A str. Hall, complete genome. |
|---|---|
| Accession | NC_009698 |
| Length | 3,760,560 |
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The map label for this gene is ispH [H]
Identifier: 153937572
GI number: 153937572
Start: 1855455
End: 1857371
Strand: Reverse
Name: ispH [H]
Synonym: CLC_1749
Alternate gene names: 153937572
Gene position: 1857371-1855455 (Counterclockwise)
Preceding gene: 153936766
Following gene: 153935267
Centisome position: 49.39
GC content: 27.91
Gene sequence:
>1917_bases ATGAATGTAATTTTAGCAGATAAAGCAGGATTTTGCTTTGGTGTTAAAAGGGCTTTAGATACAACAATAAATACCAAAGA AAATTTAAAGGGTAAAATTTATACTTTAGGTCCTTTGATACATAATAATGATGTGGTTAATCTTTTAAAAGACAAAGGTA TAGAACCTATAAGTATACAGGATGTAGATAAACTTAACAAAGATGATACTATTATTATAAGATCTCATGGAGTACCACTT CAAACTATAAACTATTTAAAAGACAAAGGGTTAAATGTTATAAATACAACCTGCCCTCATGTGGCCAATATACAAATTAA AGCTAAAAAATATTATGAAGAAGGTTACAAAATAGTAATAGTTGGAGATGAAAATCATCCAGAGGTTATTGGGATAAATG GTTGGTGTAATAATTCTGCTATTATCTCAAAGGATGGTGCCAATATAAATAACTTAGATAAAAAAGTTTGCGTATTGTGT CAAACCACAGAAAAACAAGAAAATTGGGAAAAGGTCCTTACTATATTAATTAAAAACTCTAGAGAAATATTAGCTTTTAA TACTATATGTAATGCAACACAAGTGCGCCAACAATCTGCAAAAAAATTATCTGAAAAAGTGGATAGCATGATAGTTATAG GTGGTAAAAATAGTTCTAATACTACTAAGCTTTATGAAATATGTAAAAGTAATTGCAAAAATACAATACATGTTGAAAAT GCAAAGGAAATACCTGAGCACATATATAAAAACTCAAATATTATAGGCGTTACTGCCGGAGCATCTACACCAGATTGGAT TATAAAGGAGGCTATTAATAAAATGAATAATAATGATAGTATAATAGAAGTATCAAAAAATGAAATGCTTAACTATATGA ATGAAAAGGAGCAACAAATAGTAGTTGGAAAAGTAGTAAAAGGAACTATAGTTTCTTTAAATGAAAATGAGTTATTTGTT GATTTAAGCTATAAATCTGAAGGCATTATACCTAAAGAAGAAGTAACTTTAGATGAAGAATCTATATTAAAAGAATCCTT TAAGGTAGGAGATGAAATAGAAGCCAAAATAGTTAGAATAAAAAATGAAGATGGATACGTGGTACTATCCTTAAAGGAAT TGCATCGTGAAAAAGTCTTAAAAAATTTAAAAGAAGCCTTTGAAAATAAACAAACTATAAAAGTTATTGTAAAAGATGCC GTTGATGCTGGATTAATATGTATATATAATGGTGTTAGAGTATTTATTCCAGCTTCTCATATAGAACTTTCCCATGTAGA TGACTTAGAGGCATATAAAGGCTTAGAATTAGAAGTTAATATCATTGAATTTATAAAAGATAGGTATAGAACTAAAATAG TAGGTTCAAGAAGAAACATATTAAAAACTATTAGAAATAAACATATAGAAGAAACTTGGAACTCTTTAGAAAAAGATACA GTAGTAGAAGGAGAAGTAAAGAGATTCACAGACTTTGGAGCTTTTATAGAAATTAACGGAGTGGATGGTTTACTACATGT TTCAGAAATATCCTGGGGAAGAGTAGAAAAGCCAGAAGATGCATTAAAAATAGGAGATAAAATAAAAGTATATATTTTAG ATATTGATAAGGAAAATAAAAAACTAGCTTTAAGCATAAAAAAATTAATACAAGATCCTTGGAAAAGTGTAGAGATAAAA TATCCAATAGGAAATATAGTATTAGGAAAAGTAGTTAGATTTGCAAACTTTGGAGCTTTTATAGAATTAGAACCTGGTGT AGATGGCCTAGTGCATATATCAAAAATAAGCAATAAAAGAATAGATAAACCAGAAGAAGAGTTAACTTTAGGAAAAGAAG TCAAAGCTAAAATACTAGAAGTTAATTCAGTTGAAAAAAGAATTGCATTAAGTATAAAAGACGTTGAAGAATTTTAA
Upstream 100 bases:
>100_bases AGTTGATAAAATATGTACCATAATAAAAAAAGATTAAGAAACAATCATGAATCCTATAAAAAAAGCAATATTAAAACTAA TGAAGTTAGGAGAATAGAAG
Downstream 100 bases:
>100_bases TATTGTTTAAAGATTTATTTTTAAAATCAATATTATTTTAAATTATAATTTAAATGTGTAAAGTATATGTATTAGATGAA AATATTAATACATATATTTT
Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/S1 RNA-binding domain protein
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 638; Mature: 638
Protein sequence:
>638_residues MNVILADKAGFCFGVKRALDTTINTKENLKGKIYTLGPLIHNNDVVNLLKDKGIEPISIQDVDKLNKDDTIIIRSHGVPL QTINYLKDKGLNVINTTCPHVANIQIKAKKYYEEGYKIVIVGDENHPEVIGINGWCNNSAIISKDGANINNLDKKVCVLC QTTEKQENWEKVLTILIKNSREILAFNTICNATQVRQQSAKKLSEKVDSMIVIGGKNSSNTTKLYEICKSNCKNTIHVEN AKEIPEHIYKNSNIIGVTAGASTPDWIIKEAINKMNNNDSIIEVSKNEMLNYMNEKEQQIVVGKVVKGTIVSLNENELFV DLSYKSEGIIPKEEVTLDEESILKESFKVGDEIEAKIVRIKNEDGYVVLSLKELHREKVLKNLKEAFENKQTIKVIVKDA VDAGLICIYNGVRVFIPASHIELSHVDDLEAYKGLELEVNIIEFIKDRYRTKIVGSRRNILKTIRNKHIEETWNSLEKDT VVEGEVKRFTDFGAFIEINGVDGLLHVSEISWGRVEKPEDALKIGDKIKVYILDIDKENKKLALSIKKLIQDPWKSVEIK YPIGNIVLGKVVRFANFGAFIELEPGVDGLVHISKISNKRIDKPEEELTLGKEVKAKILEVNSVEKRIALSIKDVEEF
Sequences:
>Translated_638_residues MNVILADKAGFCFGVKRALDTTINTKENLKGKIYTLGPLIHNNDVVNLLKDKGIEPISIQDVDKLNKDDTIIIRSHGVPL QTINYLKDKGLNVINTTCPHVANIQIKAKKYYEEGYKIVIVGDENHPEVIGINGWCNNSAIISKDGANINNLDKKVCVLC QTTEKQENWEKVLTILIKNSREILAFNTICNATQVRQQSAKKLSEKVDSMIVIGGKNSSNTTKLYEICKSNCKNTIHVEN AKEIPEHIYKNSNIIGVTAGASTPDWIIKEAINKMNNNDSIIEVSKNEMLNYMNEKEQQIVVGKVVKGTIVSLNENELFV DLSYKSEGIIPKEEVTLDEESILKESFKVGDEIEAKIVRIKNEDGYVVLSLKELHREKVLKNLKEAFENKQTIKVIVKDA VDAGLICIYNGVRVFIPASHIELSHVDDLEAYKGLELEVNIIEFIKDRYRTKIVGSRRNILKTIRNKHIEETWNSLEKDT VVEGEVKRFTDFGAFIEINGVDGLLHVSEISWGRVEKPEDALKIGDKIKVYILDIDKENKKLALSIKKLIQDPWKSVEIK YPIGNIVLGKVVRFANFGAFIELEPGVDGLVHISKISNKRIDKPEEELTLGKEVKAKILEVNSVEKRIALSIKDVEEF >Mature_638_residues MNVILADKAGFCFGVKRALDTTINTKENLKGKIYTLGPLIHNNDVVNLLKDKGIEPISIQDVDKLNKDDTIIIRSHGVPL QTINYLKDKGLNVINTTCPHVANIQIKAKKYYEEGYKIVIVGDENHPEVIGINGWCNNSAIISKDGANINNLDKKVCVLC QTTEKQENWEKVLTILIKNSREILAFNTICNATQVRQQSAKKLSEKVDSMIVIGGKNSSNTTKLYEICKSNCKNTIHVEN AKEIPEHIYKNSNIIGVTAGASTPDWIIKEAINKMNNNDSIIEVSKNEMLNYMNEKEQQIVVGKVVKGTIVSLNENELFV DLSYKSEGIIPKEEVTLDEESILKESFKVGDEIEAKIVRIKNEDGYVVLSLKELHREKVLKNLKEAFENKQTIKVIVKDA VDAGLICIYNGVRVFIPASHIELSHVDDLEAYKGLELEVNIIEFIKDRYRTKIVGSRRNILKTIRNKHIEETWNSLEKDT VVEGEVKRFTDFGAFIEINGVDGLLHVSEISWGRVEKPEDALKIGDKIKVYILDIDKENKKLALSIKKLIQDPWKSVEIK YPIGNIVLGKVVRFANFGAFIELEPGVDGLVHISKISNKRIDKPEEELTLGKEVKAKILEVNSVEKRIALSIKDVEEF
Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]
COG id: COG0539
COG function: function code J; Ribosomal protein S1
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 3 S1 motif domains [H]
Homologues:
Organism=Escherichia coli, GI1787140, Length=340, Percent_Identity=31.7647058823529, Blast_Score=201, Evalue=1e-52, Organism=Escherichia coli, GI1786212, Length=287, Percent_Identity=29.616724738676, Blast_Score=140, Evalue=3e-34, Organism=Escherichia coli, GI145693187, Length=75, Percent_Identity=46.6666666666667, Blast_Score=71, Evalue=2e-13, Organism=Escherichia coli, GI87082262, Length=79, Percent_Identity=43.0379746835443, Blast_Score=66, Evalue=8e-12, Organism=Saccharomyces cerevisiae, GI6323885, Length=255, Percent_Identity=23.921568627451, Blast_Score=69, Evalue=2e-12,
Paralogues:
None
Copy number: 16,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003451 - InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR003029 - InterPro: IPR000110 - InterPro: IPR022967 [H]
Pfam domain/function: PF02401 LYTB; PF00575 S1 [H]
EC number: =1.17.1.2 [H]
Molecular weight: Translated: 71921; Mature: 71921
Theoretical pI: Translated: 6.94; Mature: 6.94
Prosite motif: PS50126 S1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 0.8 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 0.8 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNVILADKAGFCFGVKRALDTTINTKENLKGKIYTLGPLIHNNDVVNLLKDKGIEPISIQ CCEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCEEECCCHHHHHHHCCCCCCCCC DVDKLNKDDTIIIRSHGVPLQTINYLKDKGLNVINTTCPHVANIQIKAKKYYEEGYKIVI CHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCEEEEEEEEEEHHHCCCEEEE VGDENHPEVIGINGWCNNSAIISKDGANINNLDKKVCVLCQTTEKQENWEKVLTILIKNS EECCCCCCEEEECCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCC REILAFNTICNATQVRQQSAKKLSEKVDSMIVIGGKNSSNTTKLYEICKSNCKNTIHVEN CEEEEEEHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEECC AKEIPEHIYKNSNIIGVTAGASTPDWIIKEAINKMNNNDSIIEVSKNEMLNYMNEKEQQI HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCHHHEE VVGKVVKGTIVSLNENELFVDLSYKSEGIIPKEEVTLDEESILKESFKVGDEIEAKIVRI EEEEEEECEEEEECCCEEEEEEECCCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEEEEEE KNEDGYVVLSLKELHREKVLKNLKEAFENKQTIKVIVKDAVDAGLICIYNGVRVFIPASH ECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCEEEEEEECC IELSHVDDLEAYKGLELEVNIIEFIKDRYRTKIVGSRRNILKTIRNKHIEETWNSLEKDT EEECCCCCHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC VVEGEVKRFTDFGAFIEINGVDGLLHVSEISWGRVEKPEDALKIGDKIKVYILDIDKENK CCCCHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHCCCEEEEEEEEECCCCC KLALSIKKLIQDPWKSVEIKYPIGNIVLGKVVRFANFGAFIELEPGVDGLVHISKISNKR HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEEHHCCCC IDKPEEELTLGKEVKAKILEVNSVEKRIALSIKDVEEF CCCCHHHHHCCHHHHHHHEECCCCCHHEEEEEHHHHCC >Mature Secondary Structure MNVILADKAGFCFGVKRALDTTINTKENLKGKIYTLGPLIHNNDVVNLLKDKGIEPISIQ CCEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCEEECCCHHHHHHHCCCCCCCCC DVDKLNKDDTIIIRSHGVPLQTINYLKDKGLNVINTTCPHVANIQIKAKKYYEEGYKIVI CHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCEEEEEEEEEEHHHCCCEEEE VGDENHPEVIGINGWCNNSAIISKDGANINNLDKKVCVLCQTTEKQENWEKVLTILIKNS EECCCCCCEEEECCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCC REILAFNTICNATQVRQQSAKKLSEKVDSMIVIGGKNSSNTTKLYEICKSNCKNTIHVEN CEEEEEEHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEECC AKEIPEHIYKNSNIIGVTAGASTPDWIIKEAINKMNNNDSIIEVSKNEMLNYMNEKEQQI HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCHHHEE VVGKVVKGTIVSLNENELFVDLSYKSEGIIPKEEVTLDEESILKESFKVGDEIEAKIVRI EEEEEEECEEEEECCCEEEEEEECCCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEEEEEE KNEDGYVVLSLKELHREKVLKNLKEAFENKQTIKVIVKDAVDAGLICIYNGVRVFIPASH ECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCEEEEEEECC IELSHVDDLEAYKGLELEVNIIEFIKDRYRTKIVGSRRNILKTIRNKHIEETWNSLEKDT EEECCCCCHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC VVEGEVKRFTDFGAFIEINGVDGLLHVSEISWGRVEKPEDALKIGDKIKVYILDIDKENK CCCCHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHCCCEEEEEEEEECCCCC KLALSIKKLIQDPWKSVEIKYPIGNIVLGKVVRFANFGAFIELEPGVDGLVHISKISNKR HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEEHHCCCC IDKPEEELTLGKEVKAKILEVNSVEKRIALSIKDVEEF CCCCHHHHHCCHHHHHHHEECCCCCHHEEEEEHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11466286 [H]