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The map label for this gene is adh1 [H]

Identifier: 153937364

GI number: 153937364

Start: 1758232

End: 1759398

Strand: Direct

Name: adh1 [H]

Synonym: CLC_1669

Alternate gene names: 153937364

Gene position: 1758232-1759398 (Clockwise)

Preceding gene: 153937722

Following gene: 153935790

Centisome position: 46.75

GC content: 33.42

Gene sequence:

>1167_bases
ATGGAAAGATTTACATTACCAAGAGATCTTTATTTTGGAGAAGGTTCTTTAGAAGCATTAAAAACATTAAAGGGTAAAAA
AGCCGTAGTAGTTGTAGGTGGCGGCTCAATGAAAAGATTTGGTTTTTTAGATAAAGTACAAAGTTATTTAAATGAGGCAG
ATATAGAAGTTAAACTAATTGAAGGAGTAGAACCAGATCCTTCTGTTGAAACTGTTATGAATGGTGCAGCTGTTATGAGA
GAATTTGAACCGGATTTAATAGTTTCAATAGGTGGTGGATCACCAATTGATGCAGCAAAGGCTATGTGGATATTCTATGA
GTACCCAGATTTTACATTTGAACAAGCTGTTGTACCTTTTGGCATACCAGAGTTAAGACAAAAAGCTAGATTTGTGGCTA
TACCATCAACTAGTGGGACTGCTACAGAAGTTACAGCTTTTTCAGTTATAACTGATTACAAGAAAAAAATAAAATATCCT
TTAGCTGATTTTAATTTAACTCCAGATATAGCTATAGTAGATCCAGATTTAGCTCAAACAATGCCAGCAAAATTAACAGC
TCATACTGGTATGGATGCTCTAACTCATGCTATAGAAGCTTATGTGGCAGGATTAAGATCTGTATTTTCAGACCCTCTTG
CAATGCAAGCTATAGTTATGGTAAAGGAATATTTAGTTAAATCTTATAATGGAAATAAAGAAGCAAGAGGACAAATGCAT
TTAGCTCAATGTTTAGCAGGAATGGCTTTTTCAAATGCATTACTTGGAATTACGCACTCAATGGCTCATAAAACCGGAGC
AGTATTCCATATTCCTCATGGATGTGCTAATGCAATCTTCCTGCCTTATGTAATTCAATATAATTCCGAATCCTGTGGTG
AGAGATATGCAACTATTGCAAAAAAACTTGGACTTGCAGGTGAGAATCAGGACGAATTAGTAAAATCATTAATAGAAATG
ATTAGAGAAATGAATAAAACTATGAACATACCATTAAACTTAAAAGAATACGGTATAACAGAGGAAGATTTTAAAGAAAA
TGTTAAATATATTTCTCATAATGCAGTATTGGATGCTTGTACTGGATCAAATCCAAGAGAAATTAATGATGAAACTATGG
AAAAATTATTTGCCTGCACATACTACGGAGAAGATGTAACATTTTAA

Upstream 100 bases:

>100_bases
AGTTTGTTAAATTTTTGGCATAATACTTGCTTTAAATATTTATAAAATAATAAGGTTAATAAAAATATTCTAATATTTAT
AATATGAGGAGGAGTTTTAA

Downstream 100 bases:

>100_bases
TTAAAAAACTATTTCAATAAAGCAATATAACTTTATTGTATAACTTATAACTTTAAGACTTCCTTTTTAAAAAAAGGAAG
TTTTAGAGTCGCATATTACT

Product: NADH-dependent butanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MERFTLPRDLYFGEGSLEALKTLKGKKAVVVVGGGSMKRFGFLDKVQSYLNEADIEVKLIEGVEPDPSVETVMNGAAVMR
EFEPDLIVSIGGGSPIDAAKAMWIFYEYPDFTFEQAVVPFGIPELRQKARFVAIPSTSGTATEVTAFSVITDYKKKIKYP
LADFNLTPDIAIVDPDLAQTMPAKLTAHTGMDALTHAIEAYVAGLRSVFSDPLAMQAIVMVKEYLVKSYNGNKEARGQMH
LAQCLAGMAFSNALLGITHSMAHKTGAVFHIPHGCANAIFLPYVIQYNSESCGERYATIAKKLGLAGENQDELVKSLIEM
IREMNKTMNIPLNLKEYGITEEDFKENVKYISHNAVLDACTGSNPREINDETMEKLFACTYYGEDVTF

Sequences:

>Translated_388_residues
MERFTLPRDLYFGEGSLEALKTLKGKKAVVVVGGGSMKRFGFLDKVQSYLNEADIEVKLIEGVEPDPSVETVMNGAAVMR
EFEPDLIVSIGGGSPIDAAKAMWIFYEYPDFTFEQAVVPFGIPELRQKARFVAIPSTSGTATEVTAFSVITDYKKKIKYP
LADFNLTPDIAIVDPDLAQTMPAKLTAHTGMDALTHAIEAYVAGLRSVFSDPLAMQAIVMVKEYLVKSYNGNKEARGQMH
LAQCLAGMAFSNALLGITHSMAHKTGAVFHIPHGCANAIFLPYVIQYNSESCGERYATIAKKLGLAGENQDELVKSLIEM
IREMNKTMNIPLNLKEYGITEEDFKENVKYISHNAVLDACTGSNPREINDETMEKLFACTYYGEDVTF
>Mature_388_residues
MERFTLPRDLYFGEGSLEALKTLKGKKAVVVVGGGSMKRFGFLDKVQSYLNEADIEVKLIEGVEPDPSVETVMNGAAVMR
EFEPDLIVSIGGGSPIDAAKAMWIFYEYPDFTFEQAVVPFGIPELRQKARFVAIPSTSGTATEVTAFSVITDYKKKIKYP
LADFNLTPDIAIVDPDLAQTMPAKLTAHTGMDALTHAIEAYVAGLRSVFSDPLAMQAIVMVKEYLVKSYNGNKEARGQMH
LAQCLAGMAFSNALLGITHSMAHKTGAVFHIPHGCANAIFLPYVIQYNSESCGERYATIAKKLGLAGENQDELVKSLIEM
IREMNKTMNIPLNLKEYGITEEDFKENVKYISHNAVLDACTGSNPREINDETMEKLFACTYYGEDVTF

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=403, Percent_Identity=24.8138957816377, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1787493, Length=411, Percent_Identity=41.3625304136253, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789163, Length=384, Percent_Identity=35.15625, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI48994951, Length=382, Percent_Identity=35.6020942408377, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082107, Length=387, Percent_Identity=34.8837209302326, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789386, Length=350, Percent_Identity=25.4285714285714, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17537053, Length=412, Percent_Identity=23.7864077669903, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=391, Percent_Identity=37.0843989769821, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24657991, Length=352, Percent_Identity=27.5568181818182, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 42753; Mature: 42753

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFTLPRDLYFGEGSLEALKTLKGKKAVVVVGGGSMKRFGFLDKVQSYLNEADIEVKLI
CCCCCCCCCEEECCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
EGVEPDPSVETVMNGAAVMREFEPDLIVSIGGGSPIDAAKAMWIFYEYPDFTFEQAVVPF
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHCCCCCC
GIPELRQKARFVAIPSTSGTATEVTAFSVITDYKKKIKYPLADFNLTPDIAIVDPDLAQT
CCHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHH
MPAKLTAHTGMDALTHAIEAYVAGLRSVFSDPLAMQAIVMVKEYLVKSYNGNKEARGQMH
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LAQCLAGMAFSNALLGITHSMAHKTGAVFHIPHGCANAIFLPYVIQYNSESCGERYATIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEEEECCHHHHHHHHHHH
KKLGLAGENQDELVKSLIEMIREMNKTMNIPLNLKEYGITEEDFKENVKYISHNAVLDAC
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEC
TGSNPREINDETMEKLFACTYYGEDVTF
CCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MERFTLPRDLYFGEGSLEALKTLKGKKAVVVVGGGSMKRFGFLDKVQSYLNEADIEVKLI
CCCCCCCCCEEECCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
EGVEPDPSVETVMNGAAVMREFEPDLIVSIGGGSPIDAAKAMWIFYEYPDFTFEQAVVPF
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHCCCCCC
GIPELRQKARFVAIPSTSGTATEVTAFSVITDYKKKIKYPLADFNLTPDIAIVDPDLAQT
CCHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHH
MPAKLTAHTGMDALTHAIEAYVAGLRSVFSDPLAMQAIVMVKEYLVKSYNGNKEARGQMH
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LAQCLAGMAFSNALLGITHSMAHKTGAVFHIPHGCANAIFLPYVIQYNSESCGERYATIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEEEECCHHHHHHHHHHH
KKLGLAGENQDELVKSLIEMIREMNKTMNIPLNLKEYGITEEDFKENVKYISHNAVLDAC
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEC
TGSNPREINDETMEKLFACTYYGEDVTF
CCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]