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The map label for this gene is mtbA [H]

Identifier: 153937342

GI number: 153937342

Start: 1599708

End: 1600778

Strand: Direct

Name: mtbA [H]

Synonym: CLC_1532

Alternate gene names: 153937342

Gene position: 1599708-1600778 (Clockwise)

Preceding gene: 153936561

Following gene: 153934670

Centisome position: 42.54

GC content: 32.59

Gene sequence:

>1071_bases
ATGGATACTTTTAAAGACGAAATGACTGCAAAGGAAAGATCAGAATCTTATTTTAAAGGTGAAGAAGTAGATAGATTACC
TTGTGCTATTATGTTTGAAGAAACGGCTGCGGTGTATGCAGGAATAAATACTAAGGAATATTATTTTGATGCAGATAAAA
TGCTAGAGGCAGAAGTATTTAAAGTAAGAGAGCTTGGAGCAGAAAGTGCAGGAATTAACGTAACCTTAAGAGGTATGGGA
GAAGCTCTGGGTAGTAAAATGGGCTATCCAAGTGATAGAGCTTCTTATTTAATTGATCCAGTATTAAAAGATTATAAAAT
GCTAGATAATATGGATGTGGTAAATCCTTACAGGGATGGAAGATTACCAATAATATTAAAAGCATTAGGTAAAATAAAAA
AAGAGCTAGGTAATGAAGTAAACATAGGTAGTGGAGTTTCAGGTCCTATTAGTGCAGCTTCAGCAGTTAGAGGGACAAAT
AATCTCATGAGAGATTTTGTTAAGAATAAAGAAGGTATACATAAATTATTGGATTTTATGACTGAATGTAATTTAGCTTT
TGTTAAAGCTGCCTATGAAGAATATGGATTAGTATGTGGCATAGGAGATCCTTTATCCTGTGGTAATTTAATAAGTGGTA
AGCAGTTTGAAAAGTTTGTAGAGCCATACCTTAGAAAAACTGTAGATGGTATATATAAGATAACAGGTAAGAAGCCTTCT
TTACATATATGTGGAAAAACAAAACATATATGGAGTCGTCTGGGTAAAATGAATATATCAACATTTAGTGTAGATAATTG
TGAAGATATAGGAGAACTGAAAGCTGTATTAGGGGATAAGGTATGTATAGTAGGAAATGTTGATCCTGTTTCTGTTATAA
GAAATGGAACTTTAGAAGATGTATATGATACTGTTAAATTATGTATAGAAAAGGTTGCAGACAGTCCTAAAGGATATGTA
GTAGGATCTGGCTGTGATATACCTGGTGGAGCTCCAGTAGAAAATATAAAAGCTATGATAGAAGCTACTAAAAAATATAG
TAAAGGTATTAGAATAGGTAGAGGAATTTAA

Upstream 100 bases:

>100_bases
ATTCTACTTTAAAATATAAATATATTAAAAATAAATATAAAATAAATAAAAAAAGAATAGAAATTTAATATATAAAATTT
GAAAGAAAGGTATGTTAACC

Downstream 100 bases:

>100_bases
CTCAAGAGGAGATGTAATATTAATGATAGAAATATTAAATTTACAAAAGTTTTATGGTGATACAAAAGTTTTACGTGATA
TAAATGTAGAAATAGATAAA

Product: putative methylcobalamin:coenzyme M methyltransferase

Products: CO2; Coproporphyrinogen III [C]

Alternate protein names: MT2-A; Methylcobamide:CoM methyltransferase II isozyme A [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MDTFKDEMTAKERSESYFKGEEVDRLPCAIMFEETAAVYAGINTKEYYFDADKMLEAEVFKVRELGAESAGINVTLRGMG
EALGSKMGYPSDRASYLIDPVLKDYKMLDNMDVVNPYRDGRLPIILKALGKIKKELGNEVNIGSGVSGPISAASAVRGTN
NLMRDFVKNKEGIHKLLDFMTECNLAFVKAAYEEYGLVCGIGDPLSCGNLISGKQFEKFVEPYLRKTVDGIYKITGKKPS
LHICGKTKHIWSRLGKMNISTFSVDNCEDIGELKAVLGDKVCIVGNVDPVSVIRNGTLEDVYDTVKLCIEKVADSPKGYV
VGSGCDIPGGAPVENIKAMIEATKKYSKGIRIGRGI

Sequences:

>Translated_356_residues
MDTFKDEMTAKERSESYFKGEEVDRLPCAIMFEETAAVYAGINTKEYYFDADKMLEAEVFKVRELGAESAGINVTLRGMG
EALGSKMGYPSDRASYLIDPVLKDYKMLDNMDVVNPYRDGRLPIILKALGKIKKELGNEVNIGSGVSGPISAASAVRGTN
NLMRDFVKNKEGIHKLLDFMTECNLAFVKAAYEEYGLVCGIGDPLSCGNLISGKQFEKFVEPYLRKTVDGIYKITGKKPS
LHICGKTKHIWSRLGKMNISTFSVDNCEDIGELKAVLGDKVCIVGNVDPVSVIRNGTLEDVYDTVKLCIEKVADSPKGYV
VGSGCDIPGGAPVENIKAMIEATKKYSKGIRIGRGI
>Mature_356_residues
MDTFKDEMTAKERSESYFKGEEVDRLPCAIMFEETAAVYAGINTKEYYFDADKMLEAEVFKVRELGAESAGINVTLRGMG
EALGSKMGYPSDRASYLIDPVLKDYKMLDNMDVVNPYRDGRLPIILKALGKIKKELGNEVNIGSGVSGPISAASAVRGTN
NLMRDFVKNKEGIHKLLDFMTECNLAFVKAAYEEYGLVCGIGDPLSCGNLISGKQFEKFVEPYLRKTVDGIYKITGKKPS
LHICGKTKHIWSRLGKMNISTFSVDNCEDIGELKAVLGDKVCIVGNVDPVSVIRNGTLEDVYDTVKLCIEKVADSPKGYV
VGSGCDIPGGAPVENIKAMIEATKKYSKGIRIGRGI

Specific function: Catalyzes the transfer of the methyl group from the methylated corrinoid cofactor of mtmC, mtbC and mttC to coenzyme M [H]

COG id: COG0407

COG function: function code H; Uroporphyrinogen-III decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen decarboxylase family. MtbA/mtaA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006360
- InterPro:   IPR000257 [H]

Pfam domain/function: PF01208 URO-D [H]

EC number: 4.1.1.37 [C]

Molecular weight: Translated: 38982; Mature: 38982

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTFKDEMTAKERSESYFKGEEVDRLPCAIMFEETAAVYAGINTKEYYFDADKMLEAEVF
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCCCCCEEECHHHHHHHHHH
KVRELGAESAGINVTLRGMGEALGSKMGYPSDRASYLIDPVLKDYKMLDNMDVVNPYRDG
HHHHHCCCCCCCEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RLPIILKALGKIKKELGNEVNIGSGVSGPISAASAVRGTNNLMRDFVKNKEGIHKLLDFM
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHH
TECNLAFVKAAYEEYGLVCGIGDPLSCGNLISGKQFEKFVEPYLRKTVDGIYKITGKKPS
HHCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCC
LHICGKTKHIWSRLGKMNISTFSVDNCEDIGELKAVLGDKVCIVGNVDPVSVIRNGTLED
EEECCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCHHH
VYDTVKLCIEKVADSPKGYVVGSGCDIPGGAPVENIKAMIEATKKYSKGIRIGRGI
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDTFKDEMTAKERSESYFKGEEVDRLPCAIMFEETAAVYAGINTKEYYFDADKMLEAEVF
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCCCCCEEECHHHHHHHHHH
KVRELGAESAGINVTLRGMGEALGSKMGYPSDRASYLIDPVLKDYKMLDNMDVVNPYRDG
HHHHHCCCCCCCEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RLPIILKALGKIKKELGNEVNIGSGVSGPISAASAVRGTNNLMRDFVKNKEGIHKLLDFM
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHH
TECNLAFVKAAYEEYGLVCGIGDPLSCGNLISGKQFEKFVEPYLRKTVDGIYKITGKKPS
HHCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCC
LHICGKTKHIWSRLGKMNISTFSVDNCEDIGELKAVLGDKVCIVGNVDPVSVIRNGTLED
EEECCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCHHH
VYDTVKLCIEKVADSPKGYVVGSGCDIPGGAPVENIKAMIEATKKYSKGIRIGRGI
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Reducing agents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00231 {7-COOH,} 0.00533 {uroporphyrinogen} 0.00256 {uroporphyrinogen} 0.00133 {uroporphyrinogen} 0.001 {uroporphyrinogen} 0.0008 {uroporphyrinogen} 0.0004 {uroporphyrinogen} 0.00035 {uroporphyrinogen} 0.00215 {5-COOH-porphyrinogen}

Substrates: H+; Uroporphyrinogen III [C]

Specific reaction: (4) H+ + Uroporphyrinogen III --> (4) CO2 + Coproporphyrinogen III [C]

General reaction: Decarboxylation [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); 7-COOH-porphyrinogenI; 7-COOH-porphyrinogen III; Butanedione; Diethyl dicarbonate; Fe2+; Iodoacetamide; p-Hydroxy mercuribenzoate; Phenyl glyoxal; Pt2+; Sn2+; UroporphyrinogenI uroporphyrinogen III; Uroporphyrinogen III;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8702528; 9642198; 7635826; 8617801; 9006042; 9195968; 10852929 [H]