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The map label for this gene is miaB

Identifier: 153936669

GI number: 153936669

Start: 1848665

End: 1850017

Strand: Reverse

Name: miaB

Synonym: CLC_1743

Alternate gene names: 153936669

Gene position: 1850017-1848665 (Counterclockwise)

Preceding gene: 153936063

Following gene: 153936560

Centisome position: 49.2

GC content: 29.56

Gene sequence:

>1353_bases
GTGAATGAAATATTAAATACAAAAGATATAAATGTTATAGGTGAATTTTTCATAGAAACCTGGGGTTGCCAAATGAATGA
AGAGGATTCAGAAAAACTTTCAGGTATGTTGAAAAAAGAGGGATATATAAGGACTGAAGAAAGAGAAAATGCAGATGTAA
TTATATTCAACACCTGCTGTGTTAGAGAAAATGCAGAACTTAAAGTATATGGTAATTTAGGGATACTTAAAGGTTTAAAA
TCTAAAAACCCAAATTTAATTATAGCTGTAACTGGTTGCATGATGCAACAAAAAGGTATGGCAGAAACCATTAAAAAGAA
ATTTCCTTTTGTAGATATTATAATAGGAACTCATAATCTGCATAACTTCCCAAATTATTTAAATGAAGTTAAGAAAAAAG
ATACATCTGTATTAAAGATACAAGAAAAAGAAAATAGCATAATAGAAAATATGCCAATAGATAGAAAAAATAGTATGAAA
GCTTTTGTTACTATAATGTATGGTTGCAATAATTTCTGTACTTATTGTATAGTTCCTTATGTAAGAGGAAGGGAAAGAAG
TAGAACCCCTGAAAATATAGAAGCTGAAATAAAGAAACTAATTTCAGAAGGTTATAAGGAAATAACTCTACTTGGCCAAA
ATGTAAATTCCTATGGTAAGGATTTAGAACCTAATGTTACATTTGCAGAGCTTTTAAAAAGAGTAAATAATATAGAAGGA
TTAGAAAGAGTAAGATTTATGACTTCCCATCCAAAGGACTTAACAGATGATGTTATAGAAGCTATTGCTAAATGTGATAA
ACTGTGTGAACAAATACATCTTCCTGTACAATCCGGTTCTAGTGAAATATTGAAAAAAATGAATAGACATTATGATAGAG
AAAAATATTTAGATGTAGTATCCAAAATAAAAAAACTTATACCTAACGTAGCACTTTCTACTGATATAATAGTAGGATTC
CCTGGAGAAACAGAAAAAGACTTTGAAGAAACATTAAGTTTAGTAAAGGAAGTGGAATACGACTCTGCTTTTACCTTCTT
ATATTCCATAAGAAAGGGAACCCCAGCTGCTAAATTTGAGGATCAAGTACCTGAAGATGTTAAACACAAGAGATTTAATA
GATTAGTAGAAGTTCTTAATGAAATAAGTGCTAAAAAGAACAAAGCCTATGAAGGAAAAATAGAAGAAGTTTTAGTAGAG
GGTACTAGTAAAAATGACGAAAATAAATTAATGGGTAGAACAAGAACAGGAAAACTTGTTAACTTTATTGGCGATAAAGA
CTCCATAGGAGAGCTTGTTAATGTTAAAATAATAAAAGCGAACTCTTTTTCATTAACAGGGGAAGAGATTTAA

Upstream 100 bases:

>100_bases
ATAACTTACTTTATATTGTGCAGTATACCATTAGAAATTATTAATAAAATATAGTATAATGTATAGGTCAAATTTTTTAA
ATTTATGAGGTGAATAAATA

Downstream 100 bases:

>100_bases
ATATAAATTAAAAAGCTCTTGAATAAGAGCTTTTTTTAAAAGATTAAATTTAAAAGGAATATTTTAAGGAGGAGAAAATA
TGGGATTAACTCCAATGATG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEIKKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGELVNVKIIKANSFSLTGEEI

Sequences:

>Translated_450_residues
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEIKKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGELVNVKIIKANSFSLTGEEI
>Mature_450_residues
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEIKKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGELVNVKIIKANSFSLTGEEI

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=490, Percent_Identity=38.1632653061225, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI28872784, Length=490, Percent_Identity=37.9591836734694, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=30.2895322939866, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1786882, Length=435, Percent_Identity=43.2183908045977, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=29.0178571428571, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=36.875, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71996771, Length=336, Percent_Identity=25.8928571428571, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21356207, Length=486, Percent_Identity=36.8312757201646, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI19922432, Length=442, Percent_Identity=30.316742081448, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_CLOB1 (A7FUL1)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384058.1
- ProteinModelPortal:   A7FUL1
- SMR:   A7FUL1
- STRING:   A7FUL1
- GeneID:   5395074
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1736
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14328
- BioCyc:   CBOT441770:CLB_1736-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51297; Mature: 51297

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEE
VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL
ECCCCCEEEEECCHHHHCCCCCCCCEEEEEECHHHHHCCHHHHHHHHCCEEEEEEECCCC
HNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY
CCHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
VRGRERSRTPENIEAEIKKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
HCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHHHCCCH
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHH
SKIKKLIPNVALSTDIIVGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFE
HHHHHHCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
DQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCHH
NFIGDKDSIGELVNVKIIKANSFSLTGEEI
EECCCCHHHCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEE
VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL
ECCCCCEEEEECCHHHHCCCCCCCCEEEEEECHHHHHCCHHHHHHHHCCEEEEEEECCCC
HNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY
CCHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
VRGRERSRTPENIEAEIKKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
HCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHHHCCCH
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHH
SKIKKLIPNVALSTDIIVGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFE
HHHHHHCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
DQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCHH
NFIGDKDSIGELVNVKIIKANSFSLTGEEI
EECCCCHHHCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA