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The map label for this gene is hupL [C]

Identifier: 153936652

GI number: 153936652

Start: 1890485

End: 1891900

Strand: Reverse

Name: hupL [C]

Synonym: CLC_1782

Alternate gene names: 153936652

Gene position: 1891900-1890485 (Counterclockwise)

Preceding gene: 153936086

Following gene: 153937639

Centisome position: 50.31

GC content: 30.01

Gene sequence:

>1416_bases
ATGGGAAAGATTATTACTATTGATCCTATAACTAGGATTAGTGGATTTTTAGAAATAAAGGTAAAGGTAGAAGACAACAT
TATTGTAGATGGTGAAACCAGCGGATTATTATTTAGAGGCTTTGAAAAAATGTTAGAAGGAAGACCTCCTTTAGATTCTA
TATATTTTACTGAAAGAATATGTGGCATATGTTCTACTGCCCATGCTACGGCATCTACTTTAGCTTTAGAAAATGCATTA
AATATAACTCCAAGTATAAATGATATATATATAAGAGATATGATGCATGGATTTGAATTTATACAAAACCACCTAAGGCA
GTTTTATTGTTTTACTGTGCCAAGCTATGTAAACCTACCTAATATAAGACCTATATACCCTCAGGAATATAGTGATTTTA
GATTGCCTAAAAAGCTAAATGAAAGAATAAGTTCCCATTATGAAGAGGGCATAAAATATAGTAGGTTAGCCCATGAAGCC
TTAGCTGTATTGGGAGGTAAAGCACCTCATAGTCATGGTATTTTCGTAGGAGGAGTTACTGTAAATATAGATTCTTACAA
ATTAACAAAAATACAATCCATATTAAAGGCAATAAAAAAGTTTGTAAATATCTATATGTTAGAGGATATGAATATAATTG
CTCAGTATTACCCTGAATATTTTAACCTTGGAAATACTTATGGAAATTTTATGAGTTTTGGTGTATTTGATAAATATAAG
GATAAAAATATAAGCTATGTAAAACCGGGAGTAATAATAAATAATAAATTAGAAAATTTTGATAGAGATAAAATAACAGA
AAACATTCGCTATGCTTGGTATGTAAGCGATACTATAATAGAAAATCCGATTATGAACAATGAATATACTGTAGATTTAA
AAAAAGAAGGAGCTTATAGTTTCGTAAAAGCACCTAGATATGCTGATTTACCTATGGAAGTAGGTCCTCTAGCTCGATTA
AAAATTGCAGGAGAATATACCGGAGGCAATTCCTCTATGGATAGAAATATAGCCCGTGTTCTAGAAACTAAAAAAATAAT
AAATATATTATATAATATATCAGAAAAAATTCAATTACTATCTAATAATCAATCTGCATATAATATTCCAGAAGGCGCCT
TTGGAGAAGGTCTTATAGATACTACTAGGGGAGCCTTATCACACTTTATAAAAATAGAGAATAAAACTATAAAGTACTAT
AATATTATAACTCCTACAGCTTGGAATATATCTCCTAAGGATTCAAAGGGTACAAGAGGTGTAGGAGAAAAATCCTTAAT
AGGCTCAAAAATAGATAATATAGAAAAACCCGTAGAAATAGGAAGAATAATTCGTTCTTTTGATCCTTGCATTTCCTGTG
CCACCCATTTAGTAAGTAATAATAATGAACCAGTTAAAGTTGAAGTATTAGTATAG

Upstream 100 bases:

>100_bases
TTCTATAGTTTAAAAAATAAAGTATATTAATTTTTTAGTTTAAATTATTTATGCTTAAGATAACTAAGTAAATTTTGAAT
TATAAAAATGGGAGGTCATT

Downstream 100 bases:

>100_bases
CTATTAAAGTTATTGCTATAGGAAATATTTTAATGGGTGATGATGGTATAGCCATAAAAATAGCTAAAAATATTGAGGGA
TTTTTGAAAGAAAATCATAT

Product: [Ni/Fe] hydrogenase, large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MGKIITIDPITRISGFLEIKVKVEDNIIVDGETSGLLFRGFEKMLEGRPPLDSIYFTERICGICSTAHATASTLALENAL
NITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLPNIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEA
LAVLGGKAPHSHGIFVGGVTVNIDSYKLTKIQSILKAIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYK
DKNISYVKPGVIINNKLENFDRDKITENIRYAWYVSDTIIENPIMNNEYTVDLKKEGAYSFVKAPRYADLPMEVGPLARL
KIAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLLSNNQSAYNIPEGAFGEGLIDTTRGALSHFIKIENKTIKYY
NIITPTAWNISPKDSKGTRGVGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLVSNNNEPVKVEVLV

Sequences:

>Translated_471_residues
MGKIITIDPITRISGFLEIKVKVEDNIIVDGETSGLLFRGFEKMLEGRPPLDSIYFTERICGICSTAHATASTLALENAL
NITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLPNIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEA
LAVLGGKAPHSHGIFVGGVTVNIDSYKLTKIQSILKAIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYK
DKNISYVKPGVIINNKLENFDRDKITENIRYAWYVSDTIIENPIMNNEYTVDLKKEGAYSFVKAPRYADLPMEVGPLARL
KIAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLLSNNQSAYNIPEGAFGEGLIDTTRGALSHFIKIENKTIKYY
NIITPTAWNISPKDSKGTRGVGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLVSNNNEPVKVEVLV
>Mature_470_residues
GKIITIDPITRISGFLEIKVKVEDNIIVDGETSGLLFRGFEKMLEGRPPLDSIYFTERICGICSTAHATASTLALENALN
ITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLPNIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEAL
AVLGGKAPHSHGIFVGGVTVNIDSYKLTKIQSILKAIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYKD
KNISYVKPGVIINNKLENFDRDKITENIRYAWYVSDTIIENPIMNNEYTVDLKKEGAYSFVKAPRYADLPMEVGPLARLK
IAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLLSNNQSAYNIPEGAFGEGLIDTTRGALSHFIKIENKTIKYYN
IITPTAWNISPKDSKGTRGVGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLVSNNNEPVKVEVLV

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=571, Percent_Identity=28.3712784588441, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI1787207, Length=583, Percent_Identity=28.1303602058319, Blast_Score=220, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 53301; Mature: 53170

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIITIDPITRISGFLEIKVKVEDNIIVDGETSGLLFRGFEKMLEGRPPLDSIYFTERI
CCCEEEECCHHHCCCEEEEEEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
CGICSTAHATASTLALENALNITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLP
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
NIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEALAVLGGKAPHSHGIFVGGVT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
VNIDSYKLTKIQSILKAIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYK
EEECCCHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
DKNISYVKPGVIINNKLENFDRDKITENIRYAWYVSDTIIENPIMNNEYTVDLKKEGAYS
CCCCCEECCCEEECCCHHCCCHHHHHCCCEEEEEEECHHHCCCCCCCEEEEEEECCCCCH
FVKAPRYADLPMEVGPLARLKIAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLL
HHCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNNQSAYNIPEGAFGEGLIDTTRGALSHFIKIENKTIKYYNIITPTAWNISPKDSKGTRG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCCCCCCCC
VGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLVSNNNEPVKVEVLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
GKIITIDPITRISGFLEIKVKVEDNIIVDGETSGLLFRGFEKMLEGRPPLDSIYFTERI
CCEEEECCHHHCCCEEEEEEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
CGICSTAHATASTLALENALNITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLP
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
NIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEALAVLGGKAPHSHGIFVGGVT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
VNIDSYKLTKIQSILKAIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYK
EEECCCHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
DKNISYVKPGVIINNKLENFDRDKITENIRYAWYVSDTIIENPIMNNEYTVDLKKEGAYS
CCCCCEECCCEEECCCHHCCCHHHHHCCCEEEEEEECHHHCCCCCCCEEEEEEECCCCCH
FVKAPRYADLPMEVGPLARLKIAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLL
HHCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNNQSAYNIPEGAFGEGLIDTTRGALSHFIKIENKTIKYYNIITPTAWNISPKDSKGTRG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCCCCCCCC
VGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLVSNNNEPVKVEVLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]