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The map label for this gene is hisZ [H]

Identifier: 153935651

GI number: 153935651

Start: 1667604

End: 1668839

Strand: Direct

Name: hisZ [H]

Synonym: CLC_1596

Alternate gene names: 153935651

Gene position: 1667604-1668839 (Clockwise)

Preceding gene: 153936999

Following gene: 153934487

Centisome position: 44.34

GC content: 25.57

Gene sequence:

>1236_bases
ATGAAAGATATCTTATTTGAAGAGAGCAATATAAAGTTAAATATAGAGGATCAATTAAGAAAAATTTATAAATATAGTGG
ATTTTCTGAAATAATATCTCCTACCATAGAATTTTATGATGTATTTAATTCTAATATTCAAGCTATACCTCAAGAAAAAA
TGTATAAACTCTTTGATAATTTAGGTAGAATTCTTGTTTTAAGACCAGATATGACTACTCCGATAGGGAGAATTACAGGC
ACTAAAATGAAGGATTGTACATATCCACTTAAACTTTGTTATACAGCAAATATATTTAGAGTTAATGAAAAATTAAATGG
AAAGCGAGGAGAAATAGCTCAATCAGGAATTGAAATAATTGGGACTAATGGAATAAAATCAGATGTGGATAGTATAGTTA
CAGCTATAAATGCTCTTTTAAGCTTAGGTCTTAAAAATTTCAAAATAGAACTAGGAGAGGCTGGATTTTTTCAGGCTCTA
ACAGAAAACATGGAAATAAAAGAGGAAAATTTAAAAAAATTAAAAGAAATAATAAGAAATAAGAACTATGTGGCTCTTAA
AAAATTTTTAGATGAAATAAGTTCAAAGTATTCAAAGGAGGATTTTGAACTTATAAAAAATCTGCCTAAATTATTTGGAG
ATATTAAAATTATAGAAAAGGCTAAGGCTTTAACTAAAAATAAAAAAGCTTTAAAATCCTTAGATGACATATATAACATA
TATAAATCCATAGAAAATATAGGACTTGAAGCATATATATCCATTGATCTTGGAATGGTTCAAAACATAGATTACTATAC
AGGGGTTATATTTAAAGGTTATGTAGAAGAAGTAGGAGATTCTATATTAAGTGGAGGAAGATATGATAATTTAATTCAAC
ATTTTGGAATAGAACTACCTGCTACAGGATTTGCTATTAATGTAGATGATATAATGATTGCTTTAAAGAAACAAAATACA
ATATCTATGGATAAAGACAAGAAGGTTTTAATATTTTACAAAGAGGAGTTTTTAAGAAAAGCATATGATTTTATGCAAGA
GCTAAAAATGAAAAAAATTATATGTGAATTAAGTCTTTTAGATAATGATAAAGAGAATTTATTATACTCAAAGAAGAAAG
GTATAGATTTTATTATAGGGTTTATGGGAGAAGAAAAACTATTTGTTAAGGATTTAAAATCAGATAAAATAGCTTTTCTA
AAGAAAGATGAGATTGAAAATTTGTTAATGTTGTAA

Upstream 100 bases:

>100_bases
ATAATATATTTTTAATTTATCACTTTATCCGAATAAACTAATAAAGTAGTAAAGCGAGGGAGACAGAATGGAAAATTGAA
ATAAGTATATACCGGAAGGT

Downstream 100 bases:

>100_bases
TTAATAAGGAAATGGGTGATTACATTGAAAAATGTAAAAATAGCGTTAACCAAAGGTAGACTTGAAAAGAAAGCTATTGA
AATATTTAAAACTATTAATA

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKDILFEESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAIPQEKMYKLFDNLGRILVLRPDMTTPIGRITG
TKMKDCTYPLKLCYTANIFRVNEKLNGKRGEIAQSGIEIIGTNGIKSDVDSIVTAINALLSLGLKNFKIELGEAGFFQAL
TENMEIKEENLKKLKEIIRNKNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKKALKSLDDIYNI
YKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYDNLIQHFGIELPATGFAINVDDIMIALKKQNT
ISMDKDKKVLIFYKEEFLRKAYDFMQELKMKKIICELSLLDNDKENLLYSKKKGIDFIIGFMGEEKLFVKDLKSDKIAFL
KKDEIENLLML

Sequences:

>Translated_411_residues
MKDILFEESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAIPQEKMYKLFDNLGRILVLRPDMTTPIGRITG
TKMKDCTYPLKLCYTANIFRVNEKLNGKRGEIAQSGIEIIGTNGIKSDVDSIVTAINALLSLGLKNFKIELGEAGFFQAL
TENMEIKEENLKKLKEIIRNKNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKKALKSLDDIYNI
YKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYDNLIQHFGIELPATGFAINVDDIMIALKKQNT
ISMDKDKKVLIFYKEEFLRKAYDFMQELKMKKIICELSLLDNDKENLLYSKKKGIDFIIGFMGEEKLFVKDLKSDKIAFL
KKDEIENLLML
>Mature_411_residues
MKDILFEESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAIPQEKMYKLFDNLGRILVLRPDMTTPIGRITG
TKMKDCTYPLKLCYTANIFRVNEKLNGKRGEIAQSGIEIIGTNGIKSDVDSIVTAINALLSLGLKNFKIELGEAGFFQAL
TENMEIKEENLKKLKEIIRNKNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKKALKSLDDIYNI
YKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYDNLIQHFGIELPATGFAINVDDIMIALKKQNT
ISMDKDKKVLIFYKEEFLRKAYDFMQELKMKKIICELSLLDNDKENLLYSKKKGIDFIIGFMGEEKLFVKDLKSDKIAFL
KKDEIENLLML

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=363, Percent_Identity=24.2424242424242, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI6996014, Length=415, Percent_Identity=21.4457831325301, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1788861, Length=330, Percent_Identity=25.7575757575758, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=373, Percent_Identity=24.9329758713137, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24643061, Length=387, Percent_Identity=22.9974160206718, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24643059, Length=335, Percent_Identity=24.1791044776119, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 47211; Mature: 47211

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDILFEESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAIPQEKMYKLFDN
CCCCEEECCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
LGRILVLRPDMTTPIGRITGTKMKDCTYPLKLCYTANIFRVNEKLNGKRGEIAQSGIEII
CCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHEEECEEEECHHCCCCCCHHHHCCCEEE
GTNGIKSDVDSIVTAINALLSLGLKNFKIELGEAGFFQALTENMEIKEENLKKLKEIIRN
ECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHCC
KNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKKALKSLDDIYNI
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYDNLIQHFGIELP
HHHHHHCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
ATGFAINVDDIMIALKKQNTISMDKDKKVLIFYKEEFLRKAYDFMQELKMKKIICELSLL
CCCEEEEHHHHEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHC
DNDKENLLYSKKKGIDFIIGFMGEEKLFVKDLKSDKIAFLKKDEIENLLML
CCCHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure
MKDILFEESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAIPQEKMYKLFDN
CCCCEEECCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
LGRILVLRPDMTTPIGRITGTKMKDCTYPLKLCYTANIFRVNEKLNGKRGEIAQSGIEII
CCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHEEECEEEECHHCCCCCCHHHHCCCEEE
GTNGIKSDVDSIVTAINALLSLGLKNFKIELGEAGFFQALTENMEIKEENLKKLKEIIRN
ECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHCC
KNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKKALKSLDDIYNI
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYDNLIQHFGIELP
HHHHHHCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
ATGFAINVDDIMIALKKQNTISMDKDKKVLIFYKEEFLRKAYDFMQELKMKKIICELSLL
CCCEEEEHHHHEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHC
DNDKENLLYSKKKGIDFIIGFMGEEKLFVKDLKSDKIAFLKKDEIENLLML
CCCHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA