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The map label for this gene is folE

Identifier: 153935134

GI number: 153935134

Start: 1659839

End: 1660429

Strand: Direct

Name: folE

Synonym: CLC_1588

Alternate gene names: 153935134

Gene position: 1659839-1660429 (Clockwise)

Preceding gene: 153937587

Following gene: 153936103

Centisome position: 44.14

GC content: 31.81

Gene sequence:

>591_bases
ATGGCAATTGATGTTAAAGCAATTGAAGAACACATAAGGGGAATTTTGATAGCTTTAGGAGACAACCCAGAAAGAGAAGG
TCTAAAGAACACACCAAAACGTGTAGCTAAAATGTATGAAGAAGTATTCAAAGGTATGTGTTATAGCAATGATGAAATTG
CAGAAATGTTTAATGTAACCTTTGAAGATGATTTATGTATAAATGATAATGAAAATGACATGGTTTTTATGAAGGAAATA
GAAATATTTAGTCATTGTGAACATCATTTAGCACTTATGTACAATATGAAAGTAGCCATAGCATATATACCTAAGAAAAA
AATTATTGGTTTAAGCAAAATAGCACGAATAGCAGATATGGTAGGACGTAGACTACAGCTTCAAGAGAGGATTGGAAGTG
ATATAGCAGAAATACTTCAGAAGATAACTGACTCAGAAGATGTAGCTGTTATTATAGAAGGAGAACATGGCTGTATGACT
ACTAGAGGAATAAAGAAGCCAGGTACTAAAACTATAACAACTACATTAAGGGGAAAGTTTAACACAGATCCTATTGTAAG
CAATAAATTAATGATGCTTTATACGAAATAA

Upstream 100 bases:

>100_bases
TATTGTAGAATTTATGAAAAATAATAGAATGAATGGAGTTAATTTACAACTGCAGCTTCACAAGATTATATGGGAGCCTA
GTAAGAGAGGAGTATAGCAT

Downstream 100 bases:

>100_bases
AGAAATTTTTAGTTCTAGTTAGAGTTTTATTAAAATAAATATATTAAATTCGTACAAGATTAAAAAATCTTACACATGAG
TAGAACAGTTTATATAAAGA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MAIDVKAIEEHIRGILIALGDNPEREGLKNTPKRVAKMYEEVFKGMCYSNDEIAEMFNVTFEDDLCINDNENDMVFMKEI
EIFSHCEHHLALMYNMKVAIAYIPKKKIIGLSKIARIADMVGRRLQLQERIGSDIAEILQKITDSEDVAVIIEGEHGCMT
TRGIKKPGTKTITTTLRGKFNTDPIVSNKLMMLYTK

Sequences:

>Translated_196_residues
MAIDVKAIEEHIRGILIALGDNPEREGLKNTPKRVAKMYEEVFKGMCYSNDEIAEMFNVTFEDDLCINDNENDMVFMKEI
EIFSHCEHHLALMYNMKVAIAYIPKKKIIGLSKIARIADMVGRRLQLQERIGSDIAEILQKITDSEDVAVIIEGEHGCMT
TRGIKKPGTKTITTTLRGKFNTDPIVSNKLMMLYTK
>Mature_195_residues
AIDVKAIEEHIRGILIALGDNPEREGLKNTPKRVAKMYEEVFKGMCYSNDEIAEMFNVTFEDDLCINDNENDMVFMKEIE
IFSHCEHHLALMYNMKVAIAYIPKKKIIGLSKIARIADMVGRRLQLQERIGSDIAEILQKITDSEDVAVIIEGEHGCMTT
RGIKKPGTKTITTTLRGKFNTDPIVSNKLMMLYTK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=179, Percent_Identity=38.5474860335196, Blast_Score=133, Evalue=9e-32,
Organism=Homo sapiens, GI4503949, Length=179, Percent_Identity=38.5474860335196, Blast_Score=133, Evalue=9e-32,
Organism=Homo sapiens, GI66932970, Length=150, Percent_Identity=36.6666666666667, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI66932972, Length=140, Percent_Identity=38.5714285714286, Blast_Score=101, Evalue=4e-22,
Organism=Escherichia coli, GI1788476, Length=176, Percent_Identity=35.7954545454545, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560486, Length=184, Percent_Identity=38.5869565217391, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321706, Length=177, Percent_Identity=43.5028248587571, Blast_Score=137, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24656782, Length=181, Percent_Identity=41.4364640883978, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24656796, Length=181, Percent_Identity=41.4364640883978, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24656791, Length=181, Percent_Identity=41.4364640883978, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_CLOB1 (A7FU67)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383900.1
- ProteinModelPortal:   A7FU67
- SMR:   A7FU67
- STRING:   A7FU67
- GeneID:   5397550
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1577
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   LTTTFTG
- ProtClustDB:   PRK09347
- BioCyc:   CBOT441770:CLB_1577-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 22270; Mature: 22139

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDVKAIEEHIRGILIALGDNPEREGLKNTPKRVAKMYEEVFKGMCYSNDEIAEMFNVT
CCCCHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
FEDDLCINDNENDMVFMKEIEIFSHCEHHLALMYNMKVAIAYIPKKKIIGLSKIARIADM
CCCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHH
VGRRLQLQERIGSDIAEILQKITDSEDVAVIIEGEHGCMTTRGIKKPGTKTITTTLRGKF
HHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCEEEEEECCCC
NTDPIVSNKLMMLYTK
CCCCCCCCCEEEEEEC
>Mature Secondary Structure 
AIDVKAIEEHIRGILIALGDNPEREGLKNTPKRVAKMYEEVFKGMCYSNDEIAEMFNVT
CCCHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
FEDDLCINDNENDMVFMKEIEIFSHCEHHLALMYNMKVAIAYIPKKKIIGLSKIARIADM
CCCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHH
VGRRLQLQERIGSDIAEILQKITDSEDVAVIIEGEHGCMTTRGIKKPGTKTITTTLRGKF
HHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCEEEEEECCCC
NTDPIVSNKLMMLYTK
CCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA