| Definition | Clostridium botulinum A str. Hall, complete genome. |
|---|---|
| Accession | NC_009698 |
| Length | 3,760,560 |
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The map label for this gene is metH [H]
Identifier: 153935049
GI number: 153935049
Start: 1730825
End: 1733203
Strand: Direct
Name: metH [H]
Synonym: CLC_1650
Alternate gene names: 153935049
Gene position: 1730825-1733203 (Clockwise)
Preceding gene: 153937732
Following gene: 153935556
Centisome position: 46.03
GC content: 28.63
Gene sequence:
>2379_bases GTGAATATTAAGGATTATATAAAAGAAAATGTATTAATATTTGATGGTGCCATGGGAACAATGCTTCAAAAATTAGGGTT AAAGATTTCAGATTTGCCAGAAGAATTAAATATATTAGAACCTGAAAAAATAATAAACATACATAGAAAATATATAGAAG CAGGAGCAAAGGTTATAACAACCAATACCTTTGGAGCAAATGAAATAAAGCTTAAACAAAGTAAGTTTTCTTTAGAAAGC ATTATAGATAAAGCAATAGATAACGTGAAAAAAGCAGGGAAAAACAAAGAAATACTTATAGCATTAGATATAGGTCCAAT AGGACAGCTTTTAGAACCTATGGGAACATTAAAATTTGAAGAAGCCTATGAAATTTTTAAAAGACAAATTGTACAAGGAC AAAAAAGTGGGGCAGATATAATTTTAATAGAAACAATGACGGATCTTTATGAAGCTAAAGCTGCTATTTTGGCAGCAAAA GAAAATACAAATCTCCCTGTATTTTGCACTATGACCTTTGAAAAAAATAAGAGAACTTTTACAGGATGCACTCCATTATC TATGGTTCTTACTTTAGAGGGACTAGGGGTAGATGCTTTAGGTGTCAATTGTTCCCTAGGGCCAAATGAATTAGGAGATA TTGTAGATGAAATAATAAAATATTCAAGTATACCTATAATGGTTCAGCCTAATGCAGGTCTTCCAACTATTAAAGCGGGT AGAACTATTTATAATATTGAGCCTAAAGAATTTGCAGATTTTCAAAGGAGTATTGTAGAAAAGGGAGTAAGGATAGTAGG AGGCTGTTGTGGAACTACAGATGAATTTATAAGAGAAATTGTATATAGTCTAAAGGATATAAAGATAAAAAAGTTAAAAG AAAACAATATTTATGGAGTATGTTCTTCTACAAAATCAGTTTTAATAGAAGGAGTTAAGATAATAGGAGAAAGAATTAAT CCAACGGGTAAAAAATTATTTAAAGAAGCCCTTAGAAATAATGATACAGATTATATATTAAAAGAAGCAATAAGTCAGGT TGAATGTGGTGCAGATATATTAGATGTAAATGTGGGACTTCCAGAAATAGATGAAGAAGAAACAATGAAAAAAGTTATAA AAGAGATTCAATCTATAATTGATACACCTCTTCAAATAGATTCTAATAATCCTAAGGTTATAGAAAAAGCATTAAGAGTA TATAATGGAAAGGCTATAGTTAATTCTGTAAATGGAGAAGAGAAAATACTAGACAGCGTATTACCATTAATAAAAAAATA TGGAGCTTCTGTTGTTGGATTAACTCTAGATGATAAAGGTATACCTAAAAAAGCTGAAGAAAGATTAAAAATAGCTGAGA AAATAGTTAATAAAGCATTAGATTATGGTATAAAAAGAAAAGATATATTTATAGATTGTTTAGTTTTAACTGCATCAGCA CAACAAGAAGATGTTAGAGAAACTCTTAAAGCGGTGACTTTAGTTAAAGAAAAACTAAATGTGAAAACAATACTAGGAGT ATCTAATATATCCTTTGGATTACCTAATAGAGAACTTATAAATAAAACTTTTTTAGCTATGAGTCTTCAATCAGGATTAG ATTTACCTATATTAAATCCTAATAATAAAGAAATGATAAATATTATAAATGCCTATAAGGTTTTAAATAATGAGGACAAG AGAGCTGCAAATTATATAGAAAGGTATACCAATGAAATATCAAATAGTAGAGAAGTAAAAATCCCAAAAAACGATTTAAC TTTAAAGGAAATAGTTATGAAAGGAATAAAAGAGGAATCATATAGTAAGACAAAGGATCTTCTTAAAGATAGAGGTGAAC TTTCAATAATAAATGAAGAACTAATTCCTGCTCTAGATGAAGTTGGTGATAAATATGAAAAAGGTATAATATTTTTACCA CAGCTAATTCAATCTGCGGAAACGGTTAAAAAAGCTTTTACAGCTATAAAAGAAAAACTTAGAGAAGATAATTCACCAAA AATAAATAAAGGTAAAATATTAATGGCAACTGTTAAAGGAGATATTCATGATATAGGAAAAAATATAGTGAAGGTTATAC TAGAAAACTATGGATTTGATATTATAGATTTAGGTAAGGATGTTGAAGCAGAAAAAATAGTAGAAGAAGTAAAGAAAAAC AATATAAAATTAGTAGGACTAAGTGCGTTGATGACTACTACAGTAAATAGTATGAGAGATACCATAAAGATTTTAAAAGA AAGTGGTATGGATTGTAAAGTTTTTGTTGGTGGAGCAGTGCTTAATGAGGAATATGCAAAAATGATAAATGCGGATTATT ATGCTAAAGATGCAAAGGAAGCAGTGGATATAGCTAAAGGATTCTTTGGAGGATTTTAA
Upstream 100 bases:
>100_bases ATTATAGGTATAATTCCAAAGGAAATTGTAGTAAATAAAAAATCTTGTAGTAAGTGCAATAAATTTAGTAGTTGTAAATT TAGAAAGATAGGTGAGATAT
Downstream 100 bases:
>100_bases ATTAAATTATACGATAATTGCCTGCTTTAATATTTCTATATTCTTAAAAATTGGAGTAATATCCATGAACAACGATTTCC TAAGCTTTAGTAAGAGTAAA
Product: putative 5-methyltetrahydrofolate--homocysteine methyltransferase
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 792; Mature: 792
Protein sequence:
>792_residues MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIEAGAKVITTNTFGANEIKLKQSKFSLES IIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFEEAYEIFKRQIVQGQKSGADIILIETMTDLYEAKAAILAAK ENTNLPVFCTMTFEKNKRTFTGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDIKIKKLKENNIYGVCSSTKSVLIEGVKIIGERIN PTGKKLFKEALRNNDTDYILKEAISQVECGADILDVNVGLPEIDEEETMKKVIKEIQSIIDTPLQIDSNNPKVIEKALRV YNGKAIVNSVNGEEKILDSVLPLIKKYGASVVGLTLDDKGIPKKAEERLKIAEKIVNKALDYGIKRKDIFIDCLVLTASA QQEDVRETLKAVTLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNPNNKEMINIINAYKVLNNEDK RAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEESYSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLP QLIQSAETVKKAFTAIKEKLREDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN NIKLVGLSALMTTTVNSMRDTIKILKESGMDCKVFVGGAVLNEEYAKMINADYYAKDAKEAVDIAKGFFGGF
Sequences:
>Translated_792_residues MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIEAGAKVITTNTFGANEIKLKQSKFSLES IIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFEEAYEIFKRQIVQGQKSGADIILIETMTDLYEAKAAILAAK ENTNLPVFCTMTFEKNKRTFTGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDIKIKKLKENNIYGVCSSTKSVLIEGVKIIGERIN PTGKKLFKEALRNNDTDYILKEAISQVECGADILDVNVGLPEIDEEETMKKVIKEIQSIIDTPLQIDSNNPKVIEKALRV YNGKAIVNSVNGEEKILDSVLPLIKKYGASVVGLTLDDKGIPKKAEERLKIAEKIVNKALDYGIKRKDIFIDCLVLTASA QQEDVRETLKAVTLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNPNNKEMINIINAYKVLNNEDK RAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEESYSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLP QLIQSAETVKKAFTAIKEKLREDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN NIKLVGLSALMTTTVNSMRDTIKILKESGMDCKVFVGGAVLNEEYAKMINADYYAKDAKEAVDIAKGFFGGF >Mature_792_residues MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIEAGAKVITTNTFGANEIKLKQSKFSLES IIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFEEAYEIFKRQIVQGQKSGADIILIETMTDLYEAKAAILAAK ENTNLPVFCTMTFEKNKRTFTGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDIKIKKLKENNIYGVCSSTKSVLIEGVKIIGERIN PTGKKLFKEALRNNDTDYILKEAISQVECGADILDVNVGLPEIDEEETMKKVIKEIQSIIDTPLQIDSNNPKVIEKALRV YNGKAIVNSVNGEEKILDSVLPLIKKYGASVVGLTLDDKGIPKKAEERLKIAEKIVNKALDYGIKRKDIFIDCLVLTASA QQEDVRETLKAVTLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNPNNKEMINIINAYKVLNNEDK RAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEESYSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLP QLIQSAETVKKAFTAIKEKLREDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN NIKLVGLSALMTTTVNSMRDTIKILKESGMDCKVFVGGAVLNEEYAKMINADYYAKDAKEAVDIAKGFFGGF
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=879, Percent_Identity=28.3276450511945, Blast_Score=283, Evalue=7e-76, Organism=Homo sapiens, GI157266337, Length=305, Percent_Identity=24.9180327868852, Blast_Score=67, Evalue=5e-11, Organism=Escherichia coli, GI1790450, Length=871, Percent_Identity=28.9322617680827, Blast_Score=304, Evalue=2e-83, Organism=Escherichia coli, GI1786456, Length=295, Percent_Identity=28.4745762711864, Blast_Score=89, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17535405, Length=885, Percent_Identity=29.1525423728814, Blast_Score=298, Evalue=8e-81, Organism=Saccharomyces cerevisiae, GI6322966, Length=322, Percent_Identity=25.1552795031056, Blast_Score=69, Evalue=2e-12,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 87940; Mature: 87940
Theoretical pI: Translated: 5.93; Mature: 5.93
Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIEAGAKVIT CCCHHHHHCCEEEEECHHHHHHHHHCCCHHCCCHHCCCCCHHHHHHHHHHHHHCCCEEEE TNTFGANEIKLKQSKFSLESIIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFE ECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCHH EAYEIFKRQIVQGQKSGADIILIETMTDLYEAKAAILAAKENTNLPVFCTMTFEKNKRTF HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCCEE TGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG CCCCHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEECC RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDIKIKKLKENNIYGV CEEEECCHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEEE CSSTKSVLIEGVKIIGERINPTGKKLFKEALRNNDTDYILKEAISQVECGADILDVNVGL ECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCC PEIDEEETMKKVIKEIQSIIDTPLQIDSNNPKVIEKALRVYNGKAIVNSVNGEEKILDSV CCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHH LPLIKKYGASVVGLTLDDKGIPKKAEERLKIAEKIVNKALDYGIKRKDIFIDCLVLTASA HHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEECCC QQEDVRETLKAVTLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNP CHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC NNKEMINIINAYKVLNNEDKRAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEES CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCHHH YSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLPQLIQSAETVKKAFTAIKEKL HHHHHHHHHCCCCEEEECHHHHHHHHHHCHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHH REDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN HCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHC NIKLVGLSALMTTTVNSMRDTIKILKESGMDCKVFVGGAVLNEEYAKMINADYYAKDAKE CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHH AVDIAKGFFGGF HHHHHHHHCCCC >Mature Secondary Structure MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIEAGAKVIT CCCHHHHHCCEEEEECHHHHHHHHHCCCHHCCCHHCCCCCHHHHHHHHHHHHHCCCEEEE TNTFGANEIKLKQSKFSLESIIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFE ECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCHH EAYEIFKRQIVQGQKSGADIILIETMTDLYEAKAAILAAKENTNLPVFCTMTFEKNKRTF HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCCEE TGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG CCCCHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEECC RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDIKIKKLKENNIYGV CEEEECCHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEEE CSSTKSVLIEGVKIIGERINPTGKKLFKEALRNNDTDYILKEAISQVECGADILDVNVGL ECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCC PEIDEEETMKKVIKEIQSIIDTPLQIDSNNPKVIEKALRVYNGKAIVNSVNGEEKILDSV CCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHH LPLIKKYGASVVGLTLDDKGIPKKAEERLKIAEKIVNKALDYGIKRKDIFIDCLVLTASA HHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEECCC QQEDVRETLKAVTLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNP CHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC NNKEMINIINAYKVLNNEDKRAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEES CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCHHH YSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLPQLIQSAETVKKAFTAIKEKL HHHHHHHHHCCCCEEEECHHHHHHHHHHCHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHH REDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN HCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHC NIKLVGLSALMTTTVNSMRDTIKILKESGMDCKVFVGGAVLNEEYAKMINADYYAKDAKE CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHH AVDIAKGFFGGF HHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11234002 [H]