Click here to switch to the map view.

The map label for this gene is appA [H]

Identifier: 153934868

GI number: 153934868

Start: 1316607

End: 1318205

Strand: Reverse

Name: appA [H]

Synonym: CLC_1268

Alternate gene names: 153934868

Gene position: 1318205-1316607 (Counterclockwise)

Preceding gene: 153935630

Following gene: 153936678

Centisome position: 35.05

GC content: 30.02

Gene sequence:

>1599_bases
ATGAAAGTGAAGAAACTATTAGCCATGATATTAGTAGCCTCAACTACCTTAGTAGCTACTGCTTGTGGAAATAGCAATAG
TTCTAGCGGATCTACAGCCAAAGAAACTTCCGCCAAAGAAAATATTAAGGATGGAGGAAATTTAGTATTTTCTATCCGTG
GTGAACCAGAAATACTTAATCCGATTTATGCTTATGATAGGGATACTATGACTATGGATAATGCTCTATTTGCACCTTTA
TTTTATATTAATGGAGATAAAATTGATTACACTCTAGCTGAAGAAGTAAAACATTCAGATGACTTTTTAACTTACACAGT
AAAACTAAAAAAAGATTTAAAATGGCATGATGGTAAACCTTTAACTGCTGATGATTTAGTATTTACTATGAAACAAATAA
TGGATGAAAAACAAGATTCACCTTTTAGAAGTGCCTTTGTTATTAATGATAAACCTGTAGAGGTAAAAAAGGTAGATGAC
TTAACCATAGAGTTTAAACTACCAACGGTTCAAATGCCTTTTATGAATTCTTTAGGACAAGTATCTCCTATTCCTAAACA
TGTATTTGAAGGAGAAAAGGACATTAAAAAAAGTATTAAAAATGAAAAACCTATAGGTTCTGGTGCCTTTAGATTTAAAG
AATCTAAAAAAGGTGAAAGTATAACGCTAGAAAGATTCGATAACTATGTTGGTGGAAAACCTCACCTTGACACCATAACC
TATAGAATAATTGCTGATCCAAATTCATCTAAGGTAGCTCTTGAAAATGGCGAAGTTTCTGCTAATTATATAGATATTAG
TGGTATAAGTAAATTTGAAAAAAATGAAAAATTAAAAGTAGTTGCCTATGATGAAGGTATGGTGGATAATTTAGTCTTAA
ATTGTAAAACAAAAGGTTTAGATAAAAAAGAAGTTAGACAAGCTATAGCCTATGCTTTAAATAAAGATGATTTAATAAAT
GCTGCTTATGAAAGTGAAAAATACGCTCCTAAGGCCTATTCCCCACTACCTAAGAATGCCTTATATTATACAGAGGATGT
TACTAAATATGGCTTAAATAAAGATAAAGCAAAGGAATTACTAAAAAAATCCGGTGCTGAAAATTTAAAACTTAAGCTAG
TTTATAGAAATGATAAAAAGACTTTAGAAAATCAAGCTTTAGTAGTTAAAGAAAATTTAAAAGATATAGGAATAGATGTT
GAATTAAAGGGATTAGAAGCAAATGCCTTTTTTAAGCAAATCGATGATCCTTCTAAAGCTGATTTTGACTTGATATTTAA
TGCCTATTTAATGGGTAACGAGCCTGATGCTTATAAAGAAGTATTTATGACAAACGGAGCTTTTAACGCTTCTAGATATA
ATAATAAAAAACTAGATGACCTTTGGAATAAAGCTGCCGTAGAAACTGATAAAACAAAGAGAGAAGAAATTTATAAAACT
ATTCAAAAGGAACTTATAGAAGATATGCCTGTATACCCAATTTGCTATTCTAATGCCACAATAGCAGTAAATAAAAATGT
AGGAGGTATAAAAGAAGCAAAAACAGCTCCTATATATATGTTCCAAGACTTATCAAAACTTTATATCATAGAAGAATAA

Upstream 100 bases:

>100_bases
TTATGATTTACTTTTATTATTTAACAATTTTTTATTAAAATGGTATCATTTATTAGTAGAATGAGTAGATTAATTTTTAC
TATTAGAAAGGGGTTTTTTT

Downstream 100 bases:

>100_bases
AAATTTAATAACTAAAAGGACGGTATTAATTGGATCTACCCTTTTAATACCGTTTATTTTTAAATAAAACTGGCATTTTA
AACTTTTATAATAATTATTT

Product: putative oligopeptide ABC transporter, oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDD
LTIEFKLPTVQMPFMNSLGQVSPIPKHVFEGEKDIKKSIKNEKPIGSGAFRFKESKKGESITLERFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKTKGLDKKEVRQAIAYALNKDDLIN
AAYESEKYAPKAYSPLPKNALYYTEDVTKYGLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYIIEE

Sequences:

>Translated_532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDD
LTIEFKLPTVQMPFMNSLGQVSPIPKHVFEGEKDIKKSIKNEKPIGSGAFRFKESKKGESITLERFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKTKGLDKKEVRQAIAYALNKDDLIN
AAYESEKYAPKAYSPLPKNALYYTEDVTKYGLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYIIEE
>Mature_532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDD
LTIEFKLPTVQMPFMNSLGQVSPIPKHVFEGEKDIKKSIKNEKPIGSGAFRFKESKKGESITLERFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKTKGLDKKEVRQAIAYALNKDDLIN
AAYESEKYAPKAYSPLPKNALYYTEDVTKYGLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYIIEE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=501, Percent_Identity=27.5449101796407, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787762, Length=482, Percent_Identity=25.5186721991701, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789887, Length=486, Percent_Identity=24.0740740740741, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787052, Length=479, Percent_Identity=23.1732776617954, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787551, Length=518, Percent_Identity=23.3590733590734, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1787495, Length=543, Percent_Identity=25.0460405156538, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081878, Length=532, Percent_Identity=24.812030075188, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789397, Length=463, Percent_Identity=23.7580993520518, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082063, Length=470, Percent_Identity=21.2765957446809, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59933; Mature: 59933

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILN
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHCHHHCCCCCCCEEEEECCCCCCCC
PIYAYDRDTMTMDNALFAPLFYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKP
CHHHCCCCCEEECCCCEEEEEEECCCEEEEEHHHHHCCCCCEEEEEEEEEECCCCCCCCC
LTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDDLTIEFKLPTVQMPFMNSLGQ
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCEEEEEECCEEECHHHHCCCC
VSPIPKHVFEGEKDIKKSIKNEKPIGSGAFRFKESKKGESITLERFDNYVGGKPHLDTIT
CCCCCHHHHCCHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEHHHHHCCCCCCCCCCEEE
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKTKGL
EEEEECCCCCEEEEECCCEEEEEEEECCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCC
DKKEVRQAIAYALNKDDLINAAYESEKYAPKAYSPLPKNALYYTEDVTKYGLNKDKAKEL
CHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHCCCHHHHHHH
LKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDVELKGLEANAFFKQIDDPSKA
HHHCCCCCEEEEEEEECCCHHHHCCEEEEECCHHHCCCEEEEECCCHHHHHHHCCCCCCC
DFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
CCEEEEEEEEECCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYIIEE
HHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCEEEEECCCEEEEECC
>Mature Secondary Structure
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILN
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHCHHHCCCCCCCEEEEECCCCCCCC
PIYAYDRDTMTMDNALFAPLFYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKP
CHHHCCCCCEEECCCCEEEEEEECCCEEEEEHHHHHCCCCCEEEEEEEEEECCCCCCCCC
LTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDDLTIEFKLPTVQMPFMNSLGQ
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCEEEEEECCEEECHHHHCCCC
VSPIPKHVFEGEKDIKKSIKNEKPIGSGAFRFKESKKGESITLERFDNYVGGKPHLDTIT
CCCCCHHHHCCHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEHHHHHCCCCCCCCCCEEE
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKTKGL
EEEEECCCCCEEEEECCCEEEEEEEECCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCC
DKKEVRQAIAYALNKDDLINAAYESEKYAPKAYSPLPKNALYYTEDVTKYGLNKDKAKEL
CHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHCCCHHHHHHH
LKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDVELKGLEANAFFKQIDDPSKA
HHHCCCCCEEEEEEEECCCHHHHCCEEEEECCHHHCCCEEEEECCCHHHHHHHCCCCCCC
DFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
CCEEEEEEEEECCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYIIEE
HHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]