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The map label for this gene is rnfC [H]

Identifier: 153934307

GI number: 153934307

Start: 2495229

End: 2496539

Strand: Reverse

Name: rnfC [H]

Synonym: CLB_2361

Alternate gene names: 153934307

Gene position: 2496539-2495229 (Counterclockwise)

Preceding gene: 153931019

Following gene: 153931353

Centisome position: 64.62

GC content: 34.4

Gene sequence:

>1311_bases
ATGTCAAGTTTATACAAGTTTTATCTTAAAATGCATATAGGTGTACCTAGTGTGCCCTGTGTTAAAGAGGGGGAATTTGT
AGAAAGAGGGCAGGTTATAGCAGAGCCTAATGGATTGGGGGCTAGAATTCATAGCAGTGTATCTGGTAAGGTTTTCAAGA
TAACAGATAAGGGAATATTAATAGAAGCTTCAGAAAATCAAAGTGAAGACTTTGTTAAGATAAAAGAATGTAATAGTATT
TTAGATACAGTGTATGAAGCTGGAATAGTAGGTGCAGGTGGGGCTGGTTTTCCAACTCATGTTAAATTAAAAGCTAATAT
ACCAGATGGGTATGTAATAGCAAATTGCGCAGAGTGTGAACCAACTCTCCATCATAATGTATATTTAGCAGAAAATCACC
CTGAACTTATAATAAAAGGTATAAAGTATGCTATGAAAGCTACTAATGCAAAAAAAGCTTATATAGGAATAAAAGGTAAA
CGTAAAAAAGCTATAGAATTATTAAGGGAACATTTGAAAAATGAAAAAAATATACAAATAAAGGAAGTTATAGACATATA
TCCTTCTGGAGAAGAAAGAGCCTTAATACATTCTATATTTGGTGAATGGTTAGCACCTACACAAATACCTATAGAGGCGA
ATTGTGTAGTTTTAAATGCAGAAACTTTAGCAAATATAACAAGAGCAGTAGAAGATAGAAAGCCAGTTATAGATAAAGAC
ATAACCATTATGGGAAAATTAAAAAAAGGTATAGGACCACATGTATTTTTACAAGAACCTATAGGAAAGTCTATGAAAGA
TATGATAGAAATCTGTGGAGGCATAGATGGTGAATATGGAGAAATTATTATAGGAGGACCGCATACTGGGCTACCAGAGG
ATATAGATCAGTCTGTAATAACAAAGGTTTCAGGTGGAGCAGTAGTAACCATGGAGTTACCGGAATATAAAGGGCCAGTA
GGTCTTTTAGTTTGTGCCTGTGCTGGAGATGAAGATAGACTTAAGGATATAGCGAGTAAAATGAGAGCAGAAGTAGTTGC
TATAACTAAATGTAAAAATGTGGTGGAAGTTAAAGGCACTTATAAATGTAAAACTCCAGGGAAGTGTCCAGGTCAAGCAG
GCGCTGTTATGTATTTAAAAAGTAAAGGGGCAAAAAGAATAATAATAGCTAATTGTAGTGATTGTTCTAATACGGTTATG
GGAATAGCTCCAAAAATGAAGTTGCCTGTTTATCACCAGACGGATCATGTACTTAGAACTGTAGATTATAAGTTAACAAG
GAGACTTCCAAAAGAGAAGCTCCATAAATAA

Upstream 100 bases:

>100_bases
TTTATATAACATATTGTTATTGACATATTTCTATTATTTATTGTTACAATAGTCAAAATATGATATTATAATAAATGGGA
ATTTCCTAAGGAGTGATAAA

Downstream 100 bases:

>100_bases
AATTATTTTTAAACTTAGGAGGTATATATTATGTCAATGTCAGCAGAACATGCAGAAGAATTAAAAAATGAACCAGCAGT
AGTTTGCTGCAGAACAGAGG

Product: putative electron transport protein

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSSLYKFYLKMHIGVPSVPCVKEGEFVERGQVIAEPNGLGARIHSSVSGKVFKITDKGILIEASENQSEDFVKIKECNSI
LDTVYEAGIVGAGGAGFPTHVKLKANIPDGYVIANCAECEPTLHHNVYLAENHPELIIKGIKYAMKATNAKKAYIGIKGK
RKKAIELLREHLKNEKNIQIKEVIDIYPSGEERALIHSIFGEWLAPTQIPIEANCVVLNAETLANITRAVEDRKPVIDKD
ITIMGKLKKGIGPHVFLQEPIGKSMKDMIEICGGIDGEYGEIIIGGPHTGLPEDIDQSVITKVSGGAVVTMELPEYKGPV
GLLVCACAGDEDRLKDIASKMRAEVVAITKCKNVVEVKGTYKCKTPGKCPGQAGAVMYLKSKGAKRIIIANCSDCSNTVM
GIAPKMKLPVYHQTDHVLRTVDYKLTRRLPKEKLHK

Sequences:

>Translated_436_residues
MSSLYKFYLKMHIGVPSVPCVKEGEFVERGQVIAEPNGLGARIHSSVSGKVFKITDKGILIEASENQSEDFVKIKECNSI
LDTVYEAGIVGAGGAGFPTHVKLKANIPDGYVIANCAECEPTLHHNVYLAENHPELIIKGIKYAMKATNAKKAYIGIKGK
RKKAIELLREHLKNEKNIQIKEVIDIYPSGEERALIHSIFGEWLAPTQIPIEANCVVLNAETLANITRAVEDRKPVIDKD
ITIMGKLKKGIGPHVFLQEPIGKSMKDMIEICGGIDGEYGEIIIGGPHTGLPEDIDQSVITKVSGGAVVTMELPEYKGPV
GLLVCACAGDEDRLKDIASKMRAEVVAITKCKNVVEVKGTYKCKTPGKCPGQAGAVMYLKSKGAKRIIIANCSDCSNTVM
GIAPKMKLPVYHQTDHVLRTVDYKLTRRLPKEKLHK
>Mature_435_residues
SSLYKFYLKMHIGVPSVPCVKEGEFVERGQVIAEPNGLGARIHSSVSGKVFKITDKGILIEASENQSEDFVKIKECNSIL
DTVYEAGIVGAGGAGFPTHVKLKANIPDGYVIANCAECEPTLHHNVYLAENHPELIIKGIKYAMKATNAKKAYIGIKGKR
KKAIELLREHLKNEKNIQIKEVIDIYPSGEERALIHSIFGEWLAPTQIPIEANCVVLNAETLANITRAVEDRKPVIDKDI
TIMGKLKKGIGPHVFLQEPIGKSMKDMIEICGGIDGEYGEIIIGGPHTGLPEDIDQSVITKVSGGAVVTMELPEYKGPVG
LLVCACAGDEDRLKDIASKMRAEVVAITKCKNVVEVKGTYKCKTPGKCPGQAGAVMYLKSKGAKRIIIANCSDCSNTVMG
IAPKMKLPVYHQTDHVLRTVDYKLTRRLPKEKLHK

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=310, Percent_Identity=24.5161290322581, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 47606; Mature: 47475

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLYKFYLKMHIGVPSVPCVKEGEFVERGQVIAEPNGLGARIHSSVSGKVFKITDKGIL
CCHHHEEEHHEECCCCCCCCCCCCCHHHCCCEEECCCCCCEEEECCCCCEEEEEECCCEE
IEASENQSEDFVKIKECNSILDTVYEAGIVGAGGAGFPTHVKLKANIPDGYVIANCAECE
EEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCC
PTLHHNVYLAENHPELIIKGIKYAMKATNAKKAYIGIKGKRKKAIELLREHLKNEKNIQI
CCHHCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEE
KEVIDIYPSGEERALIHSIFGEWLAPTQIPIEANCVVLNAETLANITRAVEDRKPVIDKD
EHEEEECCCCCCHHHHHHHHHHHCCCCCCCEECCEEEEEHHHHHHHHHHHHCCCCCCCCC
ITIMGKLKKGIGPHVFLQEPIGKSMKDMIEICGGIDGEYGEIIIGGPHTGLPEDIDQSVI
CEEEEHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHH
TKVSGGAVVTMELPEYKGPVGLLVCACAGDEDRLKDIASKMRAEVVAITKCKNVVEVKGT
HEECCCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHEEEEECCCCEEEECCE
YKCKTPGKCPGQAGAVMYLKSKGAKRIIIANCSDCSNTVMGIAPKMKLPVYHQTDHVLRT
EEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEECCCCCCCCEEECCCHHHHH
VDYKLTRRLPKEKLHK
HHHHHHHCCCHHHHCC
>Mature Secondary Structure 
SSLYKFYLKMHIGVPSVPCVKEGEFVERGQVIAEPNGLGARIHSSVSGKVFKITDKGIL
CHHHEEEHHEECCCCCCCCCCCCCHHHCCCEEECCCCCCEEEECCCCCEEEEEECCCEE
IEASENQSEDFVKIKECNSILDTVYEAGIVGAGGAGFPTHVKLKANIPDGYVIANCAECE
EEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCC
PTLHHNVYLAENHPELIIKGIKYAMKATNAKKAYIGIKGKRKKAIELLREHLKNEKNIQI
CCHHCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEE
KEVIDIYPSGEERALIHSIFGEWLAPTQIPIEANCVVLNAETLANITRAVEDRKPVIDKD
EHEEEECCCCCCHHHHHHHHHHHCCCCCCCEECCEEEEEHHHHHHHHHHHHCCCCCCCCC
ITIMGKLKKGIGPHVFLQEPIGKSMKDMIEICGGIDGEYGEIIIGGPHTGLPEDIDQSVI
CEEEEHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHH
TKVSGGAVVTMELPEYKGPVGLLVCACAGDEDRLKDIASKMRAEVVAITKCKNVVEVKGT
HEECCCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHEEEEECCCCEEEECCE
YKCKTPGKCPGQAGAVMYLKSKGAKRIIIANCSDCSNTVMGIAPKMKLPVYHQTDHVLRT
EEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEECCCCCCCCEEECCCHHHHH
VDYKLTRRLPKEKLHK
HHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]