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The map label for this gene is scrFIBM [H]

Identifier: 153933974

GI number: 153933974

Start: 2584903

End: 2585850

Strand: Reverse

Name: scrFIBM [H]

Synonym: CLB_2465

Alternate gene names: 153933974

Gene position: 2585850-2584903 (Counterclockwise)

Preceding gene: 153931403

Following gene: 153933224

Centisome position: 66.93

GC content: 30.91

Gene sequence:

>948_bases
TTGATTCAAATATTAGAATTGTTTGGAGGAATCGGAGCACCTAGAAAGGCTTTGATAAATTTAGGTGTGCCAGTGAAAGC
TATAGATTATGTTGAAATAGATGAAAAAGCAGTAAGAAGTTATAACGCTATATTTCATAAAGATTTAGCATATAAAACTC
AAAGTGTTGTTGGATATAATTTAAAGCCAGACATTTTAATTCATGGAAGCCCTTGTCAAGATTTTAGTATAGCGGGGCAT
CAAAAGGGTGCTGATAAAGGAACGGAAACAAGGAGTAGCTTAATGTGGGAAACTATAAACATTATTGAGCAAATGGGTGT
ATGGAAACCTAAATATGTTATTTGGGAAAATGTGAAGAATGTTTTAAGTAAACATATGAGACATAATTTTAATAAATATT
TAGATGAAATGCAAAACATGGGATACACAAGTAATTTTGAAGTATTAAATGCATTAGATTTTGGACTCCCACAAAAGAGG
GAAAGAGTATTTACAATAAGTTGTTTAGACGGAACATTATTTAATTTTAATGCATTGGAGAGGAAGAAAGCTCCACATAT
AAGAGAATTTTTAGAAGACACACAGGAAGAAAAGTATATAGTTACCCAACCGAGTATGCTTAAAAAAATGCATGATAAGA
ATGGTAATTTTGGTGGAAGGGTTGAGGTAATAAATGATTGTTGTTCTACTATAACTACAAAGCAAATGAGATGCCCTAAT
AGTGGAGTAGTTGACTTAGGTAATGGGAAGTATAGATATTTAACTGAATTGGAATGTTGGAGATTACAAGGTTATTCAGA
TGAAGATTTTTATAATGCATTAAATGTTCATTCTGGAATACCTGGTAAATTAAATGGAGCACTATATAAACAAGCTGGCA
ATAGTATTCCAATAACAATATTGGAAGCAATTTTTAAAGTTATTACAAATGAATATATGGTAGCTTAA

Upstream 100 bases:

>100_bases
TATAATCTTGGATAGCGAAGGAGATAGCGAAGATACATTTATAGAATATGAAGAATAATACACAATTTGATTATTATGCG
ACAGAAGAGTAGGTGATAAT

Downstream 100 bases:

>100_bases
TAGTCGTAATACAAATAAATTAAGAAGGCAGGGATAAATTAAAATGTGCCAATATTGCAAAGAAGAGTATGGAAAAAACT
TTAACATTATGAAAAGCCCT

Product: DNA-cytosine methyltransferase family protein

Products: NA

Alternate protein names: M.ScrFI-B; M.ScrFIB; Cytosine-specific methyltransferase ScrFIB [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MIQILELFGGIGAPRKALINLGVPVKAIDYVEIDEKAVRSYNAIFHKDLAYKTQSVVGYNLKPDILIHGSPCQDFSIAGH
QKGADKGTETRSSLMWETINIIEQMGVWKPKYVIWENVKNVLSKHMRHNFNKYLDEMQNMGYTSNFEVLNALDFGLPQKR
ERVFTISCLDGTLFNFNALERKKAPHIREFLEDTQEEKYIVTQPSMLKKMHDKNGNFGGRVEVINDCCSTITTKQMRCPN
SGVVDLGNGKYRYLTELECWRLQGYSDEDFYNALNVHSGIPGKLNGALYKQAGNSIPITILEAIFKVITNEYMVA

Sequences:

>Translated_315_residues
MIQILELFGGIGAPRKALINLGVPVKAIDYVEIDEKAVRSYNAIFHKDLAYKTQSVVGYNLKPDILIHGSPCQDFSIAGH
QKGADKGTETRSSLMWETINIIEQMGVWKPKYVIWENVKNVLSKHMRHNFNKYLDEMQNMGYTSNFEVLNALDFGLPQKR
ERVFTISCLDGTLFNFNALERKKAPHIREFLEDTQEEKYIVTQPSMLKKMHDKNGNFGGRVEVINDCCSTITTKQMRCPN
SGVVDLGNGKYRYLTELECWRLQGYSDEDFYNALNVHSGIPGKLNGALYKQAGNSIPITILEAIFKVITNEYMVA
>Mature_315_residues
MIQILELFGGIGAPRKALINLGVPVKAIDYVEIDEKAVRSYNAIFHKDLAYKTQSVVGYNLKPDILIHGSPCQDFSIAGH
QKGADKGTETRSSLMWETINIIEQMGVWKPKYVIWENVKNVLSKHMRHNFNKYLDEMQNMGYTSNFEVLNALDFGLPQKR
ERVFTISCLDGTLFNFNALERKKAPHIREFLEDTQEEKYIVTQPSMLKKMHDKNGNFGGRVEVINDCCSTITTKQMRCPN
SGVVDLGNGKYRYLTELECWRLQGYSDEDFYNALNVHSGIPGKLNGALYKQAGNSIPITILEAIFKVITNEYMVA

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=337, Percent_Identity=24.9258160237389, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 35819; Mature: 35819

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQILELFGGIGAPRKALINLGVPVKAIDYVEIDEKAVRSYNAIFHKDLAYKTQSVVGYN
CEEHHHHHCCCCCCHHHHEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
LKPDILIHGSPCQDFSIAGHQKGADKGTETRSSLMWETINIIEQMGVWKPKYVIWENVKN
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VLSKHMRHNFNKYLDEMQNMGYTSNFEVLNALDFGLPQKRERVFTISCLDGTLFNFNALE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEECCCHHH
RKKAPHIREFLEDTQEEKYIVTQPSMLKKMHDKNGNFGGRVEVINDCCSTITTKQMRCPN
HHCCHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
SGVVDLGNGKYRYLTELECWRLQGYSDEDFYNALNVHSGIPGKLNGALYKQAGNSIPITI
CCEEECCCCCEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHH
LEAIFKVITNEYMVA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQILELFGGIGAPRKALINLGVPVKAIDYVEIDEKAVRSYNAIFHKDLAYKTQSVVGYN
CEEHHHHHCCCCCCHHHHEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
LKPDILIHGSPCQDFSIAGHQKGADKGTETRSSLMWETINIIEQMGVWKPKYVIWENVKN
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VLSKHMRHNFNKYLDEMQNMGYTSNFEVLNALDFGLPQKRERVFTISCLDGTLFNFNALE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEECCCHHH
RKKAPHIREFLEDTQEEKYIVTQPSMLKKMHDKNGNFGGRVEVINDCCSTITTKQMRCPN
HHCCHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
SGVVDLGNGKYRYLTELECWRLQGYSDEDFYNALNVHSGIPGKLNGALYKQAGNSIPITI
CCEEECCCCCEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHH
LEAIFKVITNEYMVA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA