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The map label for this gene is ycsE [H]

Identifier: 153932650

GI number: 153932650

Start: 2656259

End: 2657083

Strand: Reverse

Name: ycsE [H]

Synonym: CLB_2540

Alternate gene names: 153932650

Gene position: 2657083-2656259 (Counterclockwise)

Preceding gene: 153933857

Following gene: 153933425

Centisome position: 68.77

GC content: 23.15

Gene sequence:

>825_bases
TTGAATTACGAATTAATAGCATTAGATATGGATGGAACTTTATTAAATGATGAAAAGAAAATAACAGAAAAAACTCTTTC
TTATATAAAAGAAGCAAAAGAAAAAGGAGTTAAGGTAGTTATATCCTCTGGAAGAGTTCCAGGAGGATTAAAATTTTATG
AAAAAACTATAGCTAAAGAGGAACCTATGATTTGTGCTAATGGTGCTTTGATTTTAAATGATAAAAAAGAAACTATATAT
AATGAAGGAATTAATAAAAATACACTTTTAAATATCATAGATGTTTTAAGAAAATATAAAGATACATACTATCATTTCTA
TCATGATAATATAATGTGTACAGAAAAATTTGATTATAGTACAAAAAAAATTTATGAATTTAATAAAAATATAGAAAGAA
AATATAGAATAGAAATAAGGATAATTACAGATAGCAAGGAATACATTAAACATAGGTCTAGTGAAATAACTAAAATAGTA
GTAGTTGATAATGATTTGAAATATTTAAATAGAATCCAAAAGGAAATAGAAGATAATTTAAAAGTTAGTGTAACTAAATC
TCATATAAGCAATATAGAAATATGTAACTTTGGAATTAGTAAGGGAATAGCCTTAGAAAAATTAGCTAATTATTACAACA
TACCTATAGAAAAATGTATAGCCGTAGGTAATGATGAAAATGATATAAGTATGATAAAAAAAGCAGGTCTTGGAGTATTT
ATGAAAAACACAAGAGAAGAATTAAAGAAATATGCTGATTACATAACTTATATGGATAATAATAATGATGGGATAGCAGA
AGTAATAGAAAAATTTATATTATAA

Upstream 100 bases:

>100_bases
CATAATTTATTACTAATTAAAACTATGTGTTCTTTTTAGTTTTTATTACCTTTATTAATGGTATAATAATAAAAAATAGA
TTAATCAGGGAGGGGTAAAT

Downstream 100 bases:

>100_bases
GTTAACTCTATATTAGTTTTATATTTTAGAAAAACTACTTATAAGAGGGTGGTAAAATTATTTATAAATATACAATTATT
TATAAATATACATACTTTTC

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNYELIALDMDGTLLNDEKKITEKTLSYIKEAKEKGVKVVISSGRVPGGLKFYEKTIAKEEPMICANGALILNDKKETIY
NEGINKNTLLNIIDVLRKYKDTYYHFYHDNIMCTEKFDYSTKKIYEFNKNIERKYRIEIRIITDSKEYIKHRSSEITKIV
VVDNDLKYLNRIQKEIEDNLKVSVTKSHISNIEICNFGISKGIALEKLANYYNIPIEKCIAVGNDENDISMIKKAGLGVF
MKNTREELKKYADYITYMDNNNDGIAEVIEKFIL

Sequences:

>Translated_274_residues
MNYELIALDMDGTLLNDEKKITEKTLSYIKEAKEKGVKVVISSGRVPGGLKFYEKTIAKEEPMICANGALILNDKKETIY
NEGINKNTLLNIIDVLRKYKDTYYHFYHDNIMCTEKFDYSTKKIYEFNKNIERKYRIEIRIITDSKEYIKHRSSEITKIV
VVDNDLKYLNRIQKEIEDNLKVSVTKSHISNIEICNFGISKGIALEKLANYYNIPIEKCIAVGNDENDISMIKKAGLGVF
MKNTREELKKYADYITYMDNNNDGIAEVIEKFIL
>Mature_274_residues
MNYELIALDMDGTLLNDEKKITEKTLSYIKEAKEKGVKVVISSGRVPGGLKFYEKTIAKEEPMICANGALILNDKKETIY
NEGINKNTLLNIIDVLRKYKDTYYHFYHDNIMCTEKFDYSTKKIYEFNKNIERKYRIEIRIITDSKEYIKHRSSEITKIV
VVDNDLKYLNRIQKEIEDNLKVSVTKSHISNIEICNFGISKGIALEKLANYYNIPIEKCIAVGNDENDISMIKKAGLGVF
MKNTREELKKYADYITYMDNNNDGIAEVIEKFIL

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI2367265, Length=280, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786982, Length=271, Percent_Identity=25.4612546125461, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081790, Length=278, Percent_Identity=24.4604316546763, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787043, Length=284, Percent_Identity=24.6478873239437, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001757
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 31810; Mature: 31810

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYELIALDMDGTLLNDEKKITEKTLSYIKEAKEKGVKVVISSGRVPGGLKFYEKTIAKE
CCEEEEEEECCCCEECCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHCCC
EPMICANGALILNDKKETIYNEGINKNTLLNIIDVLRKYKDTYYHFYHDNIMCTEKFDYS
CCEEEECCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCEEEECCCCCC
TKKIYEFNKNIERKYRIEIRIITDSKEYIKHRSSEITKIVVVDNDLKYLNRIQKEIEDNL
HHHHHHHCCCCCEEEEEEEEEEECCHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCC
KVSVTKSHISNIEICNFGISKGIALEKLANYYNIPIEKCIAVGNDENDISMIKKAGLGVF
EEEEEHHHCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHCCCCHH
MKNTREELKKYADYITYMDNNNDGIAEVIEKFIL
HHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MNYELIALDMDGTLLNDEKKITEKTLSYIKEAKEKGVKVVISSGRVPGGLKFYEKTIAKE
CCEEEEEEECCCCEECCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHCCC
EPMICANGALILNDKKETIYNEGINKNTLLNIIDVLRKYKDTYYHFYHDNIMCTEKFDYS
CCEEEECCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCEEEECCCCCC
TKKIYEFNKNIERKYRIEIRIITDSKEYIKHRSSEITKIVVVDNDLKYLNRIQKEIEDNL
HHHHHHHCCCCCEEEEEEEEEEECCHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCC
KVSVTKSHISNIEICNFGISKGIALEKLANYYNIPIEKCIAVGNDENDISMIKKAGLGVF
EEEEEHHHCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHCCCCHH
MKNTREELKKYADYITYMDNNNDGIAEVIEKFIL
HHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]