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The map label for this gene is iscS-2

Identifier: 153932593

GI number: 153932593

Start: 2618588

End: 2619781

Strand: Reverse

Name: iscS-2

Synonym: CLB_2509

Alternate gene names: 153932593

Gene position: 2619781-2618588 (Counterclockwise)

Preceding gene: 153931906

Following gene: 153933866

Centisome position: 67.81

GC content: 31.83

Gene sequence:

>1194_bases
ATGAATAAACAAGTTTACATGGATTATTCAGCTACTACTTATACAAAACCAGAGGTATTAGAAGAAATGCTTCCATTTTT
TACTGAAAATTTTGGAAATCCATCTTCTTTATATTCTTTCTCAGATAAAACTAAAAAAGCTGTAAATCTAGCAAGAGAAA
GAGTATCAAAGGCATTAAATGCAGAAAAAAATGAGATATTCTTTACAAGTGGTGGTTCAGAAGCAGACAACTGGGCTATA
AAAGGAATAGCTTATGCTAATAAAAAGAAGGGAAATCATATAATCACCACTAAGATAGAACATCATGCTATATTACATAC
GGCACAATTCTTAGAAAAAGAAGGCTTCAAAGTAACTTATCTTCCAGTAGATGAGGAGGGCTTTGTAAGTGTAGAGGATA
TAAAAAATGCTATAACAGATGAAACTATCCTAGTTTCTGTAATGTTTGCTAACAACGAAATAGGAACTATAGAACCAATA
AAAGAAATAGGGGAATTATGTAAGGAAAAAAATATTTATTTCCATACAGATGCAGTTCAAGCTATAGGTCATGTAGATAT
AGATGTAAAAGATATGAATATAGATTTGCTATCTATGTCTGCTCATAAATTTTATGGACCTAAAGGAGTAGGTGCTCTAT
ATATAAAAAATGGAGTTAAAATACAAAACTTAATTCATGGTGGAGGACAAGAAAGAGGTAAAAGGGCTAGCACAGAAAAC
ACAGCTGGAATAGTAGGGCTTGGTAAGGCCATAGAATTAGCTATGGAAAATATGCCTGAAGAAAATGAAAAACTATCTAA
TTTAAGAGGAAGGTTAATAAGAGGAATAGAAGCAAGGATACCTGAAGTAAAATTAAATGGTCCTAAAGATATGAGTAGAA
GATTACCTAATAATGTTAATTTTAGTTTTATAGGTATTGAGGGGGAAACTTTATTATTGGATTTGGATATGAATGGCATA
TTTGGATCTACAGGAAGTGCTTGTGCTTCAGCATCTTTAGATCCATCCCATGTGCTTTTATCTATAGGACTACCACATGA
AACAGCTCATGGTTCTTTGAGATTAAGCTTAGGAGCTAAGAACACAGAAGAAGATATAGATTATGTATTAGAAGTACTTC
CTAAAATAATAAAACAAAGAAGAGAAATGTCACCACTTTGGGAAGATTATATGAAAAACAAGGAGGAAAAATAA

Upstream 100 bases:

>100_bases
ATAAAGGAAAGTCTAGATAATATAATGGAATCAACTACCTTACAAGATATGGTAGAAGACTATAAGAAAATAAATTCACC
AATTAAAAGGAGTGAAAATA

Downstream 100 bases:

>100_bases
TATGTATACAGAAAAGGTAATGGATCATTTTAGAAATCCTAGAAATGTAGGGGAAATAGAAGATGCTAATGGTATAGGTG
AAGTAGGAAACGCTAAATGT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKQVYMDYSATTYTKPEVLEEMLPFFTENFGNPSSLYSFSDKTKKAVNLARERVSKALNAEKNEIFFTSGGSEADNWAI
KGIAYANKKKGNHIITTKIEHHAILHTAQFLEKEGFKVTYLPVDEEGFVSVEDIKNAITDETILVSVMFANNEIGTIEPI
KEIGELCKEKNIYFHTDAVQAIGHVDIDVKDMNIDLLSMSAHKFYGPKGVGALYIKNGVKIQNLIHGGGQERGKRASTEN
TAGIVGLGKAIELAMENMPEENEKLSNLRGRLIRGIEARIPEVKLNGPKDMSRRLPNNVNFSFIGIEGETLLLDLDMNGI
FGSTGSACASASLDPSHVLLSIGLPHETAHGSLRLSLGAKNTEEDIDYVLEVLPKIIKQRREMSPLWEDYMKNKEEK

Sequences:

>Translated_397_residues
MNKQVYMDYSATTYTKPEVLEEMLPFFTENFGNPSSLYSFSDKTKKAVNLARERVSKALNAEKNEIFFTSGGSEADNWAI
KGIAYANKKKGNHIITTKIEHHAILHTAQFLEKEGFKVTYLPVDEEGFVSVEDIKNAITDETILVSVMFANNEIGTIEPI
KEIGELCKEKNIYFHTDAVQAIGHVDIDVKDMNIDLLSMSAHKFYGPKGVGALYIKNGVKIQNLIHGGGQERGKRASTEN
TAGIVGLGKAIELAMENMPEENEKLSNLRGRLIRGIEARIPEVKLNGPKDMSRRLPNNVNFSFIGIEGETLLLDLDMNGI
FGSTGSACASASLDPSHVLLSIGLPHETAHGSLRLSLGAKNTEEDIDYVLEVLPKIIKQRREMSPLWEDYMKNKEEK
>Mature_397_residues
MNKQVYMDYSATTYTKPEVLEEMLPFFTENFGNPSSLYSFSDKTKKAVNLARERVSKALNAEKNEIFFTSGGSEADNWAI
KGIAYANKKKGNHIITTKIEHHAILHTAQFLEKEGFKVTYLPVDEEGFVSVEDIKNAITDETILVSVMFANNEIGTIEPI
KEIGELCKEKNIYFHTDAVQAIGHVDIDVKDMNIDLLSMSAHKFYGPKGVGALYIKNGVKIQNLIHGGGQERGKRASTEN
TAGIVGLGKAIELAMENMPEENEKLSNLRGRLIRGIEARIPEVKLNGPKDMSRRLPNNVNFSFIGIEGETLLLDLDMNGI
FGSTGSACASASLDPSHVLLSIGLPHETAHGSLRLSLGAKNTEEDIDYVLEVLPKIIKQRREMSPLWEDYMKNKEEK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=45.7583547557841, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=37.6811594202899, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI48994898, Length=395, Percent_Identity=47.3417721518987, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1787970, Length=396, Percent_Identity=25.7575757575758, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789175, Length=394, Percent_Identity=25.6345177664975, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI25143064, Length=390, Percent_Identity=46.4102564102564, Blast_Score=356, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI17533177, Length=334, Percent_Identity=32.3353293413174, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6319831, Length=390, Percent_Identity=45.6410256410256, Blast_Score=345, Evalue=7e-96,
Organism=Drosophila melanogaster, GI20129463, Length=389, Percent_Identity=44.987146529563, Blast_Score=341, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_CLOB1 (A7FWJ9)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384810.1
- ProteinModelPortal:   A7FWJ9
- SMR:   A7FWJ9
- STRING:   A7FWJ9
- GeneID:   5396676
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2509
- eggNOG:   COG1104
- HOGENOM:   HBG635316
- OMA:   HTCQYLE
- ProtClustDB:   CLSK972241
- BioCyc:   CBOT441770:CLB_2509-MONOMER
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR03402

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44108; Mature: 44108

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQVYMDYSATTYTKPEVLEEMLPFFTENFGNPSSLYSFSDKTKKAVNLARERVSKALN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHC
AEKNEIFFTSGGSEADNWAIKGIAYANKKKGNHIITTKIEHHAILHTAQFLEKEGFKVTY
CCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEEECHHHHHHHHHHHHHCCCEEEE
LPVDEEGFVSVEDIKNAITDETILVSVMFANNEIGTIEPIKEIGELCKEKNIYFHTDAVQ
EECCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEHHHH
AIGHVDIDVKDMNIDLLSMSAHKFYGPKGVGALYIKNGVKIQNLIHGGGQERGKRASTEN
HHCCCEEEEEECCEEEEEECHHHCCCCCCCCEEEEECCCEEEHHHCCCCHHCCCCCCCCC
TAGIVGLGKAIELAMENMPEENEKLSNLRGRLIRGIEARIPEVKLNGPKDMSRRLPNNVN
CCCEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCC
FSFIGIEGETLLLDLDMNGIFGSTGSACASASLDPSHVLLSIGLPHETAHGSLRLSLGAK
EEEEEECCCEEEEEECCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCC
NTEEDIDYVLEVLPKIIKQRREMSPLWEDYMKNKEEK
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MNKQVYMDYSATTYTKPEVLEEMLPFFTENFGNPSSLYSFSDKTKKAVNLARERVSKALN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHC
AEKNEIFFTSGGSEADNWAIKGIAYANKKKGNHIITTKIEHHAILHTAQFLEKEGFKVTY
CCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEEECHHHHHHHHHHHHHCCCEEEE
LPVDEEGFVSVEDIKNAITDETILVSVMFANNEIGTIEPIKEIGELCKEKNIYFHTDAVQ
EECCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEHHHH
AIGHVDIDVKDMNIDLLSMSAHKFYGPKGVGALYIKNGVKIQNLIHGGGQERGKRASTEN
HHCCCEEEEEECCEEEEEECHHHCCCCCCCCEEEEECCCEEEHHHCCCCHHCCCCCCCCC
TAGIVGLGKAIELAMENMPEENEKLSNLRGRLIRGIEARIPEVKLNGPKDMSRRLPNNVN
CCCEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCC
FSFIGIEGETLLLDLDMNGIFGSTGSACASASLDPSHVLLSIGLPHETAHGSLRLSLGAK
EEEEEECCCEEEEEECCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCC
NTEEDIDYVLEVLPKIIKQRREMSPLWEDYMKNKEEK
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA