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The map label for this gene is glyA

Identifier: 153932337

GI number: 153932337

Start: 2653052

End: 2654293

Strand: Reverse

Name: glyA

Synonym: CLB_2536

Alternate gene names: 153932337

Gene position: 2654293-2653052 (Counterclockwise)

Preceding gene: 153933425

Following gene: 153931097

Centisome position: 68.7

GC content: 30.52

Gene sequence:

>1242_bases
ATGGACTTTACAAATTTAAAAAACACAGATCCAGAGCTATTAGACATGATAAAGAAAGAAGAGGAAAGACAAGAATATAA
TATCGAATTAATAGCTTCAGAAAATTTTACTAGTTTATCTGTAATGGAGTCTATGGGATCTTTATTGACAAATAAATATG
CAGAAGGATACCCACATAAAAGATATTATGGAGGATGTGAATTTGTAGATGAGGTAGAGGATTTAGCAAGAGAAAGATTA
AAAAAATTATTTGCTGCAGAACATGCTAATGTACAACCACATTCTGGTTCACAAGCCAATATGGCAGTTTATATGTCTGT
GCTTCAGACTGGTGATACTATATTAGGTATGGATTTATCTCATGGCGGACATTTAACTCATGGAAGTCCAGTAAATTTTT
CAGGAAAATTATATAATTTTATATCCTATGGTGTAGATAAAGAAACAGAAACTATAGATTATGAAAAATTAAAGAAAATA
GCCTTAGAAAATAGACCTAAAATGATAGTATCTGGAGCTAGTGCTTATCCTAGAATTATAGACTTTCAAAAGATAAGAGA
AATTTGTGATGAAATAGATGCTTATATGATGGTAGATATGGCTCATATAGCAGGATTAGTAGCTACAGGATTACATCCAT
CACCAGTACCTTATGCAGATTTTGTTACAACAACTACTCATAAAACTTTAAGAGGACCTAGAGGTGGGGCTATTTTATGT
AAAGAAAAATATGCAAAAGCAGTAGATAAAGCTATATTCCCAGGCATTCAAGGGGGACCATTAATGCATACTATAGCAGC
AAAAGCCGTTTGTTTTGGGGAAGCTTTAAGAGAAGATTATAAAGAATATATGCAGCAAGTTGTTAAAAATACTAAAGTCT
TAGGAGAAGAATTAAAAAATTATGGATTTAGACTAATATCTGGTGGTACAGATAACCATTTATTACTAATAGATTTAACT
AATAAAAATATAACAGGAAAAGATGCAGAAAAACTTTTAGATTCAGTAGGAATTACTGTGAATAAAAATACAATACCTTT
TGAAACTTTAAGCCCTTTTATAACTAGTGGAATTAGAATAGGAACTCCAGCGGTAACTACGAGAGGATTTAAGGAAGAAG
AAATGAAAAAAATAGCCTATTTCATGAATTATTCTATAGAACATAGGGAAGAGAATTTATCTCAAATAAAAGAGCAAATA
AAAGAAATTTGTAAAAAATACCCATTGTATCAAAATGCATAA

Upstream 100 bases:

>100_bases
AATTTTTGTAGAAACATAAAAGTTAAATTTTATTGATTATTTTAAATTTTACAACTATAATTTATAATTAATATATGTTT
ATTTTTAGGAGCGTGATAAT

Downstream 100 bases:

>100_bases
AAAATGTAGAATTTACACATAATAGATAGGGCTTATGTCCCAAAACAGGAGCATAAGCCCATATTCTTGTGATATATTGT
AGAAAAACTATATAAATTCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMESMGSLLTNKYAEGYPHKRYYGGCEFVDEVEDLARERL
KKLFAAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLSHGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYEKLKKI
ALENRPKMIVSGASAYPRIIDFQKIREICDEIDAYMMVDMAHIAGLVATGLHPSPVPYADFVTTTTHKTLRGPRGGAILC
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLT
NKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQI
KEICKKYPLYQNA

Sequences:

>Translated_413_residues
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMESMGSLLTNKYAEGYPHKRYYGGCEFVDEVEDLARERL
KKLFAAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLSHGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYEKLKKI
ALENRPKMIVSGASAYPRIIDFQKIREICDEIDAYMMVDMAHIAGLVATGLHPSPVPYADFVTTTTHKTLRGPRGGAILC
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLT
NKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQI
KEICKKYPLYQNA
>Mature_413_residues
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMESMGSLLTNKYAEGYPHKRYYGGCEFVDEVEDLARERL
KKLFAAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLSHGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYEKLKKI
ALENRPKMIVSGASAYPRIIDFQKIREICDEIDAYMMVDMAHIAGLVATGLHPSPVPYADFVTTTTHKTLRGPRGGAILC
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLT
NKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQI
KEICKKYPLYQNA

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=407, Percent_Identity=44.7174447174447, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI261862350, Length=407, Percent_Identity=44.7174447174447, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI261862348, Length=407, Percent_Identity=44.7174447174447, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI19923315, Length=407, Percent_Identity=44.7174447174447, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI261862346, Length=403, Percent_Identity=44.6650124069479, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI22547186, Length=407, Percent_Identity=44.2260442260442, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI22547189, Length=391, Percent_Identity=42.9667519181586, Blast_Score=304, Evalue=1e-82,
Organism=Escherichia coli, GI1788902, Length=411, Percent_Identity=58.1508515815085, Blast_Score=480, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI25144732, Length=406, Percent_Identity=46.7980295566502, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25144729, Length=406, Percent_Identity=46.7980295566502, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319739, Length=416, Percent_Identity=44.2307692307692, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323087, Length=427, Percent_Identity=43.7939110070258, Blast_Score=356, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24640005, Length=407, Percent_Identity=46.4373464373464, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI221329721, Length=407, Percent_Identity=46.4373464373464, Blast_Score=380, Evalue=1e-105,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_CLOB1 (A7FWM6)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384837.1
- ProteinModelPortal:   A7FWM6
- SMR:   A7FWM6
- STRING:   A7FWM6
- GeneID:   5396836
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2536
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   MALMEPG
- ProtClustDB:   PRK00011
- BioCyc:   CBOT441770:CLB_2536-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46355; Mature: 46355

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMESMGSLLTNKYAEGYPHK
CCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
RYYGGCEFVDEVEDLARERLKKLFAAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECC
HGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYEKLKKIALENRPKMIVSGASAYPRII
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCHHH
DFQKIREICDEIDAYMMVDMAHIAGLVATGLHPSPVPYADFVTTTTHKTLRGPRGGAILC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRI
CCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCEEE
GTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQIKEICKKYPLYQNA
CCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMESMGSLLTNKYAEGYPHK
CCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
RYYGGCEFVDEVEDLARERLKKLFAAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECC
HGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYEKLKKIALENRPKMIVSGASAYPRII
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCHHH
DFQKIREICDEIDAYMMVDMAHIAGLVATGLHPSPVPYADFVTTTTHKTLRGPRGGAILC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRI
CCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCEEE
GTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQIKEICKKYPLYQNA
CCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA