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The map label for this gene is argF

Identifier: 153932030

GI number: 153932030

Start: 2650448

End: 2651449

Strand: Reverse

Name: argF

Synonym: CLB_2534

Alternate gene names: 153932030

Gene position: 2651449-2650448 (Counterclockwise)

Preceding gene: 153931097

Following gene: 153932871

Centisome position: 68.63

GC content: 33.43

Gene sequence:

>1002_bases
ATGTTTAACTTAAAAAACAGAAACTTTTTAACACTAATGGATTTCACACCAAAGGAAATAAACTATTTCTTAGATTTAGC
TAGAGATTTAAAAAGAGCTAAATATACAGGAACTGAAGTACAAAGATTGAAAGGCAAAAACATTGCTTTAATATTTGAAA
AAGCTTCAACTAGAACAAGATGCGCATTTGAAGTTGGAGCAAAAGACCAAGGAGCTCATGTAACTTACTTAGGACCAACA
GGAAGCCATATTGGTAAAAAAGAATCAGCTGCAGATACTGCAAGAGTTTTAGGAAGAATGTATGATGGTATAGAATATAG
AGGATTTGGTCAAGAAATCGTTGAAACTTTAGCTGAATACGCTGGAGTACCAGTTTGGAACGGATTAACAGATGAAGATC
ACCCAACTCAAATATTAGCTGACTTCTTAACAATAAGAGAACATTTCAATAAACCATTAAATGAAATCAAATTTGCTTAT
GTTGGAGATGGTGCTAATAATATGGCAAATGCTCTTATGATAGGTGCTGTTAAAATGGGAATGGACTTTAGAATAGTTTC
TCCAAAAGAAATACCAACTGATGCTGCATTAGTTGCTAAATGCAAGGAAATAGCAGCTGAAACTGGCGCTAAAGTTACAA
TAACTGATAATATCGAAGAAGGTGTTAAAGGTTGTGACGTTCTATATACAGATGTTTGGGTATCAATGGGAGAACCAGAT
TCAGTATGGGAAAGCAAAATAAAATTATTAACTCCATACAGAGTTGACATGAACATGATAAAAATGACTGGAAATCCTGA
TGCTAAATTCATGCACTGCTTACCAGCATTCCATGATGAAGAAACAGCAGTAGGTAAAGAAATAAAAGAAAAATATGGTT
TATCAGAAATGGAAGTTTCTCACGAATTATTCGAAAGCAAATACTCAATTGTATTTGATGAAGCTGAAAACAGAATGCAC
ACTATAAAAGCTGTTATGGTTGCAACATTAGGAGATCAATAA

Upstream 100 bases:

>100_bases
AGATAATTAATTAAAAAGCTAACAGGGTTGCATAATACATATGTAATCTAAAATATAAAAGTTTATAATACAAATATTAT
ACTATTAGGAGGAATAACAT

Downstream 100 bases:

>100_bases
TCATTAAATATATAAGATAAAAGGAAGAATGGAAACATTCTTCCTTTTATTTTTTGATATACAAAAAAATGATAAAACAA
CTCTAAAACACTTGAAATTT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRLKGKNIALIFEKASTRTRCAFEVGAKDQGAHVTYLGPT
GSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEYAGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAY
VGDGANNMANALMIGAVKMGMDFRIVSPKEIPTDAALVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVSHELFESKYSIVFDEAENRMH
TIKAVMVATLGDQ

Sequences:

>Translated_333_residues
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRLKGKNIALIFEKASTRTRCAFEVGAKDQGAHVTYLGPT
GSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEYAGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAY
VGDGANNMANALMIGAVKMGMDFRIVSPKEIPTDAALVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVSHELFESKYSIVFDEAENRMH
TIKAVMVATLGDQ
>Mature_333_residues
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRLKGKNIALIFEKASTRTRCAFEVGAKDQGAHVTYLGPT
GSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEYAGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAY
VGDGANNMANALMIGAVKMGMDFRIVSPKEIPTDAALVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVSHELFESKYSIVFDEAENRMH
TIKAVMVATLGDQ

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=331, Percent_Identity=39.5770392749245, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI18105007, Length=341, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1790703, Length=332, Percent_Identity=59.6385542168675, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=58.3832335329341, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI2367364, Length=232, Percent_Identity=30.1724137931034, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994908, Length=374, Percent_Identity=24.5989304812834, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=32.6219512195122, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=26.797385620915, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24642586, Length=230, Percent_Identity=26.5217391304348, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_CLOB1 (A7FWM4)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384835.1
- ProteinModelPortal:   A7FWM4
- SMR:   A7FWM4
- STRING:   A7FWM4
- GeneID:   5396752
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2534
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   MTPYQIN
- ProtClustDB:   PRK04284
- BioCyc:   CBOT441770:CLB_2534-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 37231; Mature: 37231

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 107-107 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRLKGKNIALIFEKASTRTR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCCE
CAFEVGAKDQGAHVTYLGPTGSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEY
EEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAYVGDGANNMANALMIGAVKMG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCHHHHHHHHHHHHHHC
MDFRIVSPKEIPTDAALVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHCCCHHHEEEEEHEEECCCCH
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVS
HHHHHHHEEECCEEECCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHH
HELFESKYSIVFDEAENRMHTIKAVMVATLGDQ
HHHHHCHHEEEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRLKGKNIALIFEKASTRTR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCCE
CAFEVGAKDQGAHVTYLGPTGSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEY
EEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAYVGDGANNMANALMIGAVKMG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCHHHHHHHHHHHHHHC
MDFRIVSPKEIPTDAALVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHCCCHHHEEEEEHEEECCCCH
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVS
HHHHHHHEEECCEEECCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHH
HELFESKYSIVFDEAENRMHTIKAVMVATLGDQ
HHHHHCHHEEEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA