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The map label for this gene is def [H]

Identifier: 153931783

GI number: 153931783

Start: 2518107

End: 2518550

Strand: Reverse

Name: def [H]

Synonym: CLB_2383

Alternate gene names: 153931783

Gene position: 2518550-2518107 (Counterclockwise)

Preceding gene: 153933517

Following gene: 153931476

Centisome position: 65.19

GC content: 30.41

Gene sequence:

>444_bases
ATGGCAATAAGAAATATAAGAAAATATGGAGATGAATTATTAAGGAAGAAAAGCAGGAAAATAGAAAAGATTGATGATAG
AATTTTAACTCTTTTAGAGGATATGGTAGAAACTATGTATAATGCAGAGGGAGTAGGACTTGCAGCACCTCAAGTAGGAA
TATTAAAAAGAGCGGTAGTTATAGATGTTGGAGAAGGATTAATAAAACTTATAAATCCAGAAATAATAGAGACAGAAGGA
AATCAAAAAGATGTAGAAGGATGTTTAAGTGTTCCTGGTGAACAAGGAGAAGTAGAAAGACCATATAAAGTTAAGGTTAA
AGCTTTAAATGAAAAAGGAGAAGAAATAGTATTAGAAGGAGAAGATCTATTAGCTAGAGCCTTTTGTCATGAAATAGATC
ATTTAGATGGAGTTTTATTTGTAGATAAGGTAATAAATGATTAG

Upstream 100 bases:

>100_bases
TTATACTTCTTTAGAACAAGTTTATAATGATATAAGAGTAAGTATAGATATAAATCCGAACAGTTTGTTATAATTTTTTA
AATTTTAGGAGGTCGTAAAA

Downstream 100 bases:

>100_bases
GAAGGAGAAAATTAATTTGAAGGATACAACTATAGTTTTTATGGGTACCCCAGATTTTGCAGTACCATCTTTAAAAAAAT
TAATAGAAGAATTTAATGTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MAIRNIRKYGDELLRKKSRKIEKIDDRILTLLEDMVETMYNAEGVGLAAPQVGILKRAVVIDVGEGLIKLINPEIIETEG
NQKDVEGCLSVPGEQGEVERPYKVKVKALNEKGEEIVLEGEDLLARAFCHEIDHLDGVLFVDKVIND

Sequences:

>Translated_147_residues
MAIRNIRKYGDELLRKKSRKIEKIDDRILTLLEDMVETMYNAEGVGLAAPQVGILKRAVVIDVGEGLIKLINPEIIETEG
NQKDVEGCLSVPGEQGEVERPYKVKVKALNEKGEEIVLEGEDLLARAFCHEIDHLDGVLFVDKVIND
>Mature_146_residues
AIRNIRKYGDELLRKKSRKIEKIDDRILTLLEDMVETMYNAEGVGLAAPQVGILKRAVVIDVGEGLIKLINPEIIETEGN
QKDVEGCLSVPGEQGEVERPYKVKVKALNEKGEEIVLEGEDLLARAFCHEIDHLDGVLFVDKVIND

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=163, Percent_Identity=26.9938650306748, Blast_Score=70, Evalue=7e-13,
Organism=Escherichia coli, GI1789682, Length=145, Percent_Identity=44.1379310344828, Blast_Score=114, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=35.1515151515151, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645726, Length=166, Percent_Identity=30.7228915662651, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 16438; Mature: 16307

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRNIRKYGDELLRKKSRKIEKIDDRILTLLEDMVETMYNAEGVGLAAPQVGILKRAVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
IDVGEGLIKLINPEIIETEGNQKDVEGCLSVPGEQGEVERPYKVKVKALNEKGEEIVLEG
EECCHHHHHHHCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEEEEEC
EDLLARAFCHEIDHLDGVLFVDKVIND
HHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
AIRNIRKYGDELLRKKSRKIEKIDDRILTLLEDMVETMYNAEGVGLAAPQVGILKRAVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
IDVGEGLIKLINPEIIETEGNQKDVEGCLSVPGEQGEVERPYKVKVKALNEKGEEIVLEG
EECCHHHHHHHCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEEEEEC
EDLLARAFCHEIDHLDGVLFVDKVIND
HHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9504995; 11466286 [H]