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The map label for this gene is recG [H]

Identifier: 153931726

GI number: 153931726

Start: 2503113

End: 2505152

Strand: Reverse

Name: recG [H]

Synonym: CLB_2368

Alternate gene names: 153931726

Gene position: 2505152-2503113 (Counterclockwise)

Preceding gene: 153931483

Following gene: 153934015

Centisome position: 64.84

GC content: 27.6

Gene sequence:

>2040_bases
ATGAATGTATATAGTCCTATAACTACTTTAAAAGGAGTGGGACCTAAAACTAAAGAACAATTAGAAAAATGTATGATATT
TAACATAATGGACTTACTTTTATATTTTCCAAGGGATTATGAATTTATAGATAATTATAGCAAAGACAAACTGCTAAGTA
AAAAAGTAATAATTAAAGTACAAGTAGAAAATATAAAAAGGGATATAAGGACAAGAACAGGTAAAATTTTAACCACAATT
ATATTCAATGATGGAGAGAAAGCTATAGTTGGTAGTTGGTTTAATCAGCCTTATATTAAAAATTATTTTAAAATAGGTGA
AGAATATGTACTTCAGGGAAGCTTAAAAGAATATAGGGGGAATTTAACTATAAATAACGCTCAAATATTAAAGAACAAAT
GTGGAGAAGAGGTAGAGGAAAGAAAAATTATTCCTAAATATCCTTTAAAGGGAGACTTGAAAAATAATTTGCTAATAAAA
CTTGTAGATAGTGTTTTGACAAATATTGATATAGGTGAGAATTTACCTATATGGCTTATAGAAAAATATAAGTTTCTATC
TTTAGATAAATCCATAAGAACTATACATAAACCAGAAAATCAGAAAGAATTAGAAGAGTCTATAAGAAGATTAAAATTTC
AAGAATTATTAGCATATTGCTTAAAAATAGCTTTTTTAAAGGAATACTTAGAAACAGCTACAGAGGGTATATCTTTTATA
GTTTCAGAAGAAATAAATAATCTTATAGAAGTATTACCTTTCAAACTGACTAATGCACAAAATAAAGTTTTACAGGAAAT
ATTCAAGGACCAAGAAAAAGAAAAACCAATGAATAGGCTTTTACAGGGGGATGTAGGTAGTGGAAAAACTATTATAGCTT
TAATATCTCTTTTTAATGTAATAAAAAATGGATATCAAGGAGTAATGTTAGCACCTACAGAAATATTAGCGGTGCAACAT
TATGAAGAAGCGTTGAAATTATTTAAGGATTTTAATTTAAATATAGAGCTTTTAATAGGAAGCATAAAGGTATCTTCTAA
AAAGGAGATAAAAGAAAAGCTTAAAGAAGGGAAAATAGACTTAATAATAGGTACTCATGCTTTAATTGAAGATGATGTGG
AATTTTATAATCTAGGCATGGTTATAACAGATGAACAACACCGTTTTGGAGTTATGCAAAGAAGTAAAATGTTAAATAAG
GGAAAAAGCGTAGATACTTTAGTTATGAGTGCAACTCCTATACCAAGAAGCCTTACTTTGAGTCTATATGGAGATTTAGA
TTTATCTATTATAGATGAACTGCCACCAGGAAGACAAAAGATAGATACCTACTATGTAAATGATAGCTATAGAAAAAGGG
TGTATAATTTTGCACTAAAAGAAATAAATAATGGTAGACAGGTATATATAGTTTGCCCTCTAGTAGAAGAAAAAGAGGAA
TTGAATTTAAATTCCGTAGAAAAATTATATGATGATTTGAAAGGGGAATATTTTAAAGAAGTAGAAATTGCTATTTTACA
TGGCAAAATGAAAGGAAAAGAAAAAGATACTATTATGAAAGATTTTAAGGATGGGAAAATAAAAGCCTTAATATCCACTA
CTGTTATAGAAGTAGGGGTAAATGTACCTAATGCTACTTTAATGGTTATAGAAAATGCAGAGAGATTTGGTTTAGCTCAA
CTTCATCAATTAAGGGGAAGAGTAGGTAGGGGAAAACATAAATCCTATTGTATATTAATTGCTAGAGTGAAAAATGATAT
TATAAGAAAAAGAATGGAAATAATGAAAAGTAGTAATGATGGTTTTCTTGTAGCGGAGGAAGATCTAAAGTTAAGAGGTG
GAGGTGAAATATTTGGATTTAAACAGCATGGTTCTAGTAATTTGCTTCTGGCTGATGTAATAGAGGATATACATTTACTT
AGAATAGCTAATATGGAAAGTAAAAAAATAATAGATAGTAATAATGAAGAGGATAATAAAATAAAAGAAGAAGTAAAAAT
TAAGATAAAAAATAATTCAAAGTTTATATGTTTTAATTAG

Upstream 100 bases:

>100_bases
CTTATAGAAAATAATATTAGGAAGGGATTCTTAGTTTAAGTTAAAGTTTTTAGAAGGTTTTATTCTTTACTATAGAATCC
TTTATAATAGGAGGAAGTTT

Downstream 100 bases:

>100_bases
GAGAACAAATTTCTACAAATTACTGTAAATAATTTTGTATGGTAATAATTTATATGTTAAAATAATAAAGATTAAGTTAT
TTTTAGGATCAGATAGGTTT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MNVYSPITTLKGVGPKTKEQLEKCMIFNIMDLLLYFPRDYEFIDNYSKDKLLSKKVIIKVQVENIKRDIRTRTGKILTTI
IFNDGEKAIVGSWFNQPYIKNYFKIGEEYVLQGSLKEYRGNLTINNAQILKNKCGEEVEERKIIPKYPLKGDLKNNLLIK
LVDSVLTNIDIGENLPIWLIEKYKFLSLDKSIRTIHKPENQKELEESIRRLKFQELLAYCLKIAFLKEYLETATEGISFI
VSEEINNLIEVLPFKLTNAQNKVLQEIFKDQEKEKPMNRLLQGDVGSGKTIIALISLFNVIKNGYQGVMLAPTEILAVQH
YEEALKLFKDFNLNIELLIGSIKVSSKKEIKEKLKEGKIDLIIGTHALIEDDVEFYNLGMVITDEQHRFGVMQRSKMLNK
GKSVDTLVMSATPIPRSLTLSLYGDLDLSIIDELPPGRQKIDTYYVNDSYRKRVYNFALKEINNGRQVYIVCPLVEEKEE
LNLNSVEKLYDDLKGEYFKEVEIAILHGKMKGKEKDTIMKDFKDGKIKALISTTVIEVGVNVPNATLMVIENAERFGLAQ
LHQLRGRVGRGKHKSYCILIARVKNDIIRKRMEIMKSSNDGFLVAEEDLKLRGGGEIFGFKQHGSSNLLLADVIEDIHLL
RIANMESKKIIDSNNEEDNKIKEEVKIKIKNNSKFICFN

Sequences:

>Translated_679_residues
MNVYSPITTLKGVGPKTKEQLEKCMIFNIMDLLLYFPRDYEFIDNYSKDKLLSKKVIIKVQVENIKRDIRTRTGKILTTI
IFNDGEKAIVGSWFNQPYIKNYFKIGEEYVLQGSLKEYRGNLTINNAQILKNKCGEEVEERKIIPKYPLKGDLKNNLLIK
LVDSVLTNIDIGENLPIWLIEKYKFLSLDKSIRTIHKPENQKELEESIRRLKFQELLAYCLKIAFLKEYLETATEGISFI
VSEEINNLIEVLPFKLTNAQNKVLQEIFKDQEKEKPMNRLLQGDVGSGKTIIALISLFNVIKNGYQGVMLAPTEILAVQH
YEEALKLFKDFNLNIELLIGSIKVSSKKEIKEKLKEGKIDLIIGTHALIEDDVEFYNLGMVITDEQHRFGVMQRSKMLNK
GKSVDTLVMSATPIPRSLTLSLYGDLDLSIIDELPPGRQKIDTYYVNDSYRKRVYNFALKEINNGRQVYIVCPLVEEKEE
LNLNSVEKLYDDLKGEYFKEVEIAILHGKMKGKEKDTIMKDFKDGKIKALISTTVIEVGVNVPNATLMVIENAERFGLAQ
LHQLRGRVGRGKHKSYCILIARVKNDIIRKRMEIMKSSNDGFLVAEEDLKLRGGGEIFGFKQHGSSNLLLADVIEDIHLL
RIANMESKKIIDSNNEEDNKIKEEVKIKIKNNSKFICFN
>Mature_679_residues
MNVYSPITTLKGVGPKTKEQLEKCMIFNIMDLLLYFPRDYEFIDNYSKDKLLSKKVIIKVQVENIKRDIRTRTGKILTTI
IFNDGEKAIVGSWFNQPYIKNYFKIGEEYVLQGSLKEYRGNLTINNAQILKNKCGEEVEERKIIPKYPLKGDLKNNLLIK
LVDSVLTNIDIGENLPIWLIEKYKFLSLDKSIRTIHKPENQKELEESIRRLKFQELLAYCLKIAFLKEYLETATEGISFI
VSEEINNLIEVLPFKLTNAQNKVLQEIFKDQEKEKPMNRLLQGDVGSGKTIIALISLFNVIKNGYQGVMLAPTEILAVQH
YEEALKLFKDFNLNIELLIGSIKVSSKKEIKEKLKEGKIDLIIGTHALIEDDVEFYNLGMVITDEQHRFGVMQRSKMLNK
GKSVDTLVMSATPIPRSLTLSLYGDLDLSIIDELPPGRQKIDTYYVNDSYRKRVYNFALKEINNGRQVYIVCPLVEEKEE
LNLNSVEKLYDDLKGEYFKEVEIAILHGKMKGKEKDTIMKDFKDGKIKALISTTVIEVGVNVPNATLMVIENAERFGLAQ
LHQLRGRVGRGKHKSYCILIARVKNDIIRKRMEIMKSSNDGFLVAEEDLKLRGGGEIFGFKQHGSSNLLLADVIEDIHLL
RIANMESKKIIDSNNEEDNKIKEEVKIKIKNNSKFICFN

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=660, Percent_Identity=36.3636363636364, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1787357, Length=401, Percent_Identity=37.6558603491272, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77994; Mature: 77994

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVYSPITTLKGVGPKTKEQLEKCMIFNIMDLLLYFPRDYEFIDNYSKDKLLSKKVIIKV
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCEEEEEE
QVENIKRDIRTRTGKILTTIIFNDGEKAIVGSWFNQPYIKNYFKIGEEYVLQGSLKEYRG
EHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCHHHHHHHHCCHHHHHCCCHHHHCC
NLTINNAQILKNKCGEEVEERKIIPKYPLKGDLKNNLLIKLVDSVLTNIDIGENLPIWLI
CEEECHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
EKYKFLSLDKSIRTIHKPENQKELEESIRRLKFQELLAYCLKIAFLKEYLETATEGISFI
ECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEEINNLIEVLPFKLTNAQNKVLQEIFKDQEKEKPMNRLLQGDVGSGKTIIALISLFNV
HHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHH
IKNGYQGVMLAPTEILAVQHYEEALKLFKDFNLNIELLIGSIKVSSKKEIKEKLKEGKID
HHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHCCCEE
LIIGTHALIEDDVEFYNLGMVITDEQHRFGVMQRSKMLNKGKSVDTLVMSATPIPRSLTL
EEEECHHHHHCCCCEEEECEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEE
SLYGDLDLSIIDELPPGRQKIDTYYVNDSYRKRVYNFALKEINNGRQVYIVCPLVEEKEE
EEECCCCHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
LNLNSVEKLYDDLKGEYFKEVEIAILHGKMKGKEKDTIMKDFKDGKIKALISTTVIEVGV
CCHHHHHHHHHHHCCHHHHHEEEEEEECCCCCCCHHHHHHHCCCCCEEEHHHHHHHEECC
NVPNATLMVIENAERFGLAQLHQLRGRVGRGKHKSYCILIARVKNDIIRKRMEIMKSSND
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCC
GFLVAEEDLKLRGGGEIFGFKQHGSSNLLLADVIEDIHLLRIANMESKKIIDSNNEEDNK
CEEEEECCEEEECCCCEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
IKEEVKIKIKNNSKFICFN
HHEEEEEEEECCCEEEEEC
>Mature Secondary Structure
MNVYSPITTLKGVGPKTKEQLEKCMIFNIMDLLLYFPRDYEFIDNYSKDKLLSKKVIIKV
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCEEEEEE
QVENIKRDIRTRTGKILTTIIFNDGEKAIVGSWFNQPYIKNYFKIGEEYVLQGSLKEYRG
EHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCHHHHHHHHCCHHHHHCCCHHHHCC
NLTINNAQILKNKCGEEVEERKIIPKYPLKGDLKNNLLIKLVDSVLTNIDIGENLPIWLI
CEEECHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
EKYKFLSLDKSIRTIHKPENQKELEESIRRLKFQELLAYCLKIAFLKEYLETATEGISFI
ECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEEINNLIEVLPFKLTNAQNKVLQEIFKDQEKEKPMNRLLQGDVGSGKTIIALISLFNV
HHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHH
IKNGYQGVMLAPTEILAVQHYEEALKLFKDFNLNIELLIGSIKVSSKKEIKEKLKEGKID
HHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHCCCEE
LIIGTHALIEDDVEFYNLGMVITDEQHRFGVMQRSKMLNKGKSVDTLVMSATPIPRSLTL
EEEECHHHHHCCCCEEEECEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEE
SLYGDLDLSIIDELPPGRQKIDTYYVNDSYRKRVYNFALKEINNGRQVYIVCPLVEEKEE
EEECCCCHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
LNLNSVEKLYDDLKGEYFKEVEIAILHGKMKGKEKDTIMKDFKDGKIKALISTTVIEVGV
CCHHHHHHHHHHHCCHHHHHEEEEEEECCCCCCCHHHHHHHCCCCCEEEHHHHHHHEECC
NVPNATLMVIENAERFGLAQLHQLRGRVGRGKHKSYCILIARVKNDIIRKRMEIMKSSND
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCC
GFLVAEEDLKLRGGGEIFGFKQHGSSNLLLADVIEDIHLLRIANMESKKIIDSNNEEDNK
CEEEEECCEEEECCCCEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
IKEEVKIKIKNNSKFICFN
HHEEEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8830261; 11463916 [H]