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The map label for this gene is fmt [H]

Identifier: 153931476

GI number: 153931476

Start: 2517149

End: 2518090

Strand: Reverse

Name: fmt [H]

Synonym: CLB_2382

Alternate gene names: 153931476

Gene position: 2518090-2517149 (Counterclockwise)

Preceding gene: 153931783

Following gene: 153931020

Centisome position: 65.18

GC content: 30.15

Gene sequence:

>942_bases
TTGAAGGATACAACTATAGTTTTTATGGGTACCCCAGATTTTGCAGTACCATCTTTAAAAAAATTAATAGAAGAATTTAA
TGTAAAGGCAGTATTTACACAACCGGATAGACCAAAGGGACGAGGGAAAAAATTAGCTATGTCAGCGGTTAAGGAAGTGG
CCTTACAAAATAACATAGAGGTATATCAGCCAATAAAATTAAAAAATGATGAGATTTGTATAAAAAAGTTGAAAGAAATA
AGTCCAGATTTTATAATAGTAGTAGCTTTTGGACAAATATTATCAAAGGAAGTTTTGAATATACCAAAGTATGGATGTAT
TAATTTACATGCCTCTCTACTTCCTAAATATAGAGGAGCAGCACCTATAAACTGGGCAATAATAAAGGGAGAAAATGAAT
CTGGGAATACTACTATGTTTATGGATGAAGGTTTAGATACAGGTGATATGCTTTTAAAAAATACAGTTAAAATAGAAGAT
GATATGACCTTTGGTGAGCTTCATGATATATTAATGGAAAGTGGTTCAGAACTTTTAGTAGATACTATAAAGGGATTAAA
AGAAGGAACTATAGAAAGAGAGAAGCAAAAGAGTGAAAATATTATATATGCCTCTATGCTAAATAAACAAATGGGGAAAA
TAGATTGGAATAAAACTTCTAAGGAAATAAATTTACTTATAAGAGGGTTAAACCCACGACCAGTTGCATATACTCAATAT
AAAAATGAAACTATGAAAATTTATAACAGTAGTATATTAAAAGAAAGCTCAAATAAAACTCCAGGTACTATATTAAAGGT
ATCAAAAGAAGGTATTAAAGTAGCTACCGGTGATGGAATTTTATCTATTACAACTGTACAATTTCCTGGGAAAAAACCTA
TGAAAGTAGAAGAATATATAAAGGGACATACTATAGAAGAAGGATTAGTACTAGGAGAATAG

Upstream 100 bases:

>100_bases
GAAGATCTATTAGCTAGAGCCTTTTGTCATGAAATAGATCATTTAGATGGAGTTTTATTTGTAGATAAGGTAATAAATGA
TTAGGAAGGAGAAAATTAAT

Downstream 100 bases:

>100_bases
AAGAAGAATTTTTTATTCATGTTTAAAATATTTAGTAAGGTTAAAACTAGATATAATATATTTTTTATTCTATAATTAGT
TAGTATTAAATAAAAATAGA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKDTTIVFMGTPDFAVPSLKKLIEEFNVKAVFTQPDRPKGRGKKLAMSAVKEVALQNNIEVYQPIKLKNDEICIKKLKEI
SPDFIIVVAFGQILSKEVLNIPKYGCINLHASLLPKYRGAAPINWAIIKGENESGNTTMFMDEGLDTGDMLLKNTVKIED
DMTFGELHDILMESGSELLVDTIKGLKEGTIEREKQKSENIIYASMLNKQMGKIDWNKTSKEINLLIRGLNPRPVAYTQY
KNETMKIYNSSILKESSNKTPGTILKVSKEGIKVATGDGILSITTVQFPGKKPMKVEEYIKGHTIEEGLVLGE

Sequences:

>Translated_313_residues
MKDTTIVFMGTPDFAVPSLKKLIEEFNVKAVFTQPDRPKGRGKKLAMSAVKEVALQNNIEVYQPIKLKNDEICIKKLKEI
SPDFIIVVAFGQILSKEVLNIPKYGCINLHASLLPKYRGAAPINWAIIKGENESGNTTMFMDEGLDTGDMLLKNTVKIED
DMTFGELHDILMESGSELLVDTIKGLKEGTIEREKQKSENIIYASMLNKQMGKIDWNKTSKEINLLIRGLNPRPVAYTQY
KNETMKIYNSSILKESSNKTPGTILKVSKEGIKVATGDGILSITTVQFPGKKPMKVEEYIKGHTIEEGLVLGE
>Mature_313_residues
MKDTTIVFMGTPDFAVPSLKKLIEEFNVKAVFTQPDRPKGRGKKLAMSAVKEVALQNNIEVYQPIKLKNDEICIKKLKEI
SPDFIIVVAFGQILSKEVLNIPKYGCINLHASLLPKYRGAAPINWAIIKGENESGNTTMFMDEGLDTGDMLLKNTVKIED
DMTFGELHDILMESGSELLVDTIKGLKEGTIEREKQKSENIIYASMLNKQMGKIDWNKTSKEINLLIRGLNPRPVAYTQY
KNETMKIYNSSILKESSNKTPGTILKVSKEGIKVATGDGILSITTVQFPGKKPMKVEEYIKGHTIEEGLVLGE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=292, Percent_Identity=27.3972602739726, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21614513, Length=293, Percent_Identity=26.6211604095563, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI164663775, Length=320, Percent_Identity=26.5625, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789683, Length=296, Percent_Identity=43.9189189189189, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788589, Length=285, Percent_Identity=28.4210526315789, Blast_Score=141, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI133930964, Length=330, Percent_Identity=26.3636363636364, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45550868, Length=314, Percent_Identity=29.9363057324841, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571984, Length=232, Percent_Identity=33.1896551724138, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24585660, Length=239, Percent_Identity=26.3598326359833, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34926; Mature: 34926

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTTIVFMGTPDFAVPSLKKLIEEFNVKAVFTQPDRPKGRGKKLAMSAVKEVALQNNIE
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
VYQPIKLKNDEICIKKLKEISPDFIIVVAFGQILSKEVLNIPKYGCINLHASLLPKYRGA
EEECEEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEHHHHCCHHCCC
APINWAIIKGENESGNTTMFMDEGLDTGDMLLKNTVKIEDDMTFGELHDILMESGSELLV
CCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHCEEEECCCCCHHHHHHHHHHCCCHHHH
DTIKGLKEGTIEREKQKSENIIYASMLNKQMGKIDWNKTSKEINLLIRGLNPRPVAYTQY
HHHHHHHHCCCHHHHHHCCCEEEHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCEEEEEC
KNETMKIYNSSILKESSNKTPGTILKVSKEGIKVATGDGILSITTVQFPGKKPMKVEEYI
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCEEEEEEEECCCCCCCCHHHHH
KGHTIEEGLVLGE
CCCCCCCCCEECC
>Mature Secondary Structure
MKDTTIVFMGTPDFAVPSLKKLIEEFNVKAVFTQPDRPKGRGKKLAMSAVKEVALQNNIE
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
VYQPIKLKNDEICIKKLKEISPDFIIVVAFGQILSKEVLNIPKYGCINLHASLLPKYRGA
EEECEEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEHHHHCCHHCCC
APINWAIIKGENESGNTTMFMDEGLDTGDMLLKNTVKIEDDMTFGELHDILMESGSELLV
CCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHCEEEECCCCCHHHHHHHHHHCCCHHHH
DTIKGLKEGTIEREKQKSENIIYASMLNKQMGKIDWNKTSKEINLLIRGLNPRPVAYTQY
HHHHHHHHCCCHHHHHHCCCEEEHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCEEEEEC
KNETMKIYNSSILKESSNKTPGTILKVSKEGIKVATGDGILSITTVQFPGKKPMKVEEYI
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCEEEEEEEECCCCCCCCHHHHH
KGHTIEEGLVLGE
CCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]