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The map label for this gene is lipA [H]

Identifier: 152977249

GI number: 152977249

Start: 3628646

End: 3629542

Strand: Reverse

Name: lipA [H]

Synonym: Bcer98_3568

Alternate gene names: 152977249

Gene position: 3629542-3628646 (Counterclockwise)

Preceding gene: 152977251

Following gene: 152977246

Centisome position: 88.81

GC content: 39.24

Gene sequence:

>897_bases
ATGACAAAACAAACAGAATATAAGCGCAAGCCCGAATGGTTGAAAATTAAGTTAAATACAAATGAAAACTATACAGGCTT
AAAGAAAATGATGCGTTCTAAGCAGCTTCATACAGTTTGTGAAGAAGCAAAATGTCCGAACATTCATGAATGTTGGGCAG
TAAGAAAAACAGCAACATTTATGATTTTAGGTGCAATTTGTACACGTGCTTGCCGCTTCTGTGCGGTTAAAACAGGCTTA
CCAACAGAGCTTGATTTACAAGAGCCAGAACGCGTAGCGGATTCTGTTGTACAAATGGGATTAAAACACGTTGTTATAAC
AGCGGTTGCACGCGATGATTTAAAGGACGGAGGAGCTGCGGTTTTTGCTGAAACAGTGCGCGCAGTTCGCCGCAAAAATC
CATTTACATCTATTGAAGTGTTACCATCTGATATGGGTGGGGTAGAAGAGAATTTAAGAATATTAATGGATGCAAAACCA
GATATTTTAAACCATAACATTGAAACGGTACGTCGATTATCTGACCGAGTTCGCGCAAGAGCAAAATATGATCGTTCCTT
AGAATTTTTACGTCGTGCAAAAGAAATGCAACCTGATATTCCAACTAAATCTAGCATTATGGTGGGATTAGGAGAAACAA
GAGAAGATTTACTTGAAGCAATGGACGATTTACGTGCGAACAATGTGGATATTTTAACGCTTGGACAATATCTACAACCA
TCTAAGAAACACTTACCAGTTATTCGATATTACACACCTGCTGAATTTGCAGAGCTGAAAGAAATTGCGCTTAGCAAAGG
ATTCAGTCACTGTGAAGCGGGTCCACTTGTGCGCTCTTCTTACCATGCAGATGAGCAAGTTCGTTCTGCAAAAGAGAATA
CAGTAGAAGCAAAATAA

Upstream 100 bases:

>100_bases
ATCTTCATTTGAGCAAATCGCTTTCATACGTTAAAATAGATATGTTACATTGAAAGAAGCGAACACGGTAAACTATTTCA
TATTGTGCGGAGTTGATAAC

Downstream 100 bases:

>100_bases
TACAAAAAAGAGAGCAAATGACTAGCTCTCTTTTTTGTATTCCAAAACGGGATTTTGACATGTGGATAAAATTTTCTTTA
ATTCTTCTGTGGATAATTCG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKQLHTVCEEAKCPNIHECWAVRKTATFMILGAICTRACRFCAVKTGL
PTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLRILMDAKP
DILNHNIETVRRLSDRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLLEAMDDLRANNVDILTLGQYLQP
SKKHLPVIRYYTPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTVEAK

Sequences:

>Translated_298_residues
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKQLHTVCEEAKCPNIHECWAVRKTATFMILGAICTRACRFCAVKTGL
PTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLRILMDAKP
DILNHNIETVRRLSDRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLLEAMDDLRANNVDILTLGQYLQP
SKKHLPVIRYYTPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTVEAK
>Mature_297_residues
TKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKQLHTVCEEAKCPNIHECWAVRKTATFMILGAICTRACRFCAVKTGLP
TELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLRILMDAKPD
ILNHNIETVRRLSDRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLLEAMDDLRANNVDILTLGQYLQPS
KKHLPVIRYYTPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTVEAK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=44.9826989619377, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=45.4183266932271, Blast_Score=224, Evalue=9e-59,
Organism=Escherichia coli, GI1786846, Length=290, Percent_Identity=52.7586206896552, Blast_Score=305, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=45.8041958041958, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=47.2027972027972, Blast_Score=262, Evalue=5e-71,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.643109540636, Blast_Score=257, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33786; Mature: 33655

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKQLHTVCEEAKCPNIHECWAVRKTATF
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MILGAICTRACRFCAVKTGLPTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFAETVRAVRRKNPFTSIEVLPSDMGGVEENLRILMDAKPDILNHNIETVRRLSDRVRAR
HHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHEEEECCCCHHHCCCHHHHHHHHHHHHHH
AKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLLEAMDDLRANNVDILTLGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHCC
SKKHLPVIRYYTPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTVEAK
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKQLHTVCEEAKCPNIHECWAVRKTATF
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MILGAICTRACRFCAVKTGLPTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFAETVRAVRRKNPFTSIEVLPSDMGGVEENLRILMDAKPDILNHNIETVRRLSDRVRAR
HHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHEEEECCCCHHHCCCHHHHHHHHHHHHHH
AKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLLEAMDDLRANNVDILTLGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHCC
SKKHLPVIRYYTPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKENTVEAK
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA