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The map label for this gene is rph [H]

Identifier: 152976902

GI number: 152976902

Start: 3271400

End: 3272137

Strand: Reverse

Name: rph [H]

Synonym: Bcer98_3202

Alternate gene names: 152976902

Gene position: 3272137-3271400 (Counterclockwise)

Preceding gene: 152976903

Following gene: 152976901

Centisome position: 80.06

GC content: 40.79

Gene sequence:

>738_bases
ATGCGAGTAGATGGTAGAGAAGTAAGAGAATTACGCCATGTACATATTCATACAAATTATTTAAAACATCCAGAAGGTTC
TGTGTTAATTGAAGTTGGAGATACGAAGGTGATTTGTTCGGCGACGGTTGAAGAACGAGTACCTCCATTTATGCGCGGTG
AAGGAAAAGGCTGGGTAACAGCGGAATATGCGATGATTCCACGTGCAACGGAACAGCGTACCATTCGTGAGTCTAGTAAA
GGGAAAGTAACAGGCCGTACAATGGAAATTCAACGTCTTATTGGACGCGCGCTGCGAGCGGTTGTTGATTTAGAAGTGCT
TGGAGAAAGAACAGTGTGGATTGATTGTGATGTGATTCAAGCAGATGGTGGAACGCGAACAGCATCAATTACGGGCGCAT
ATGTCGCAATGGTATTGGCATTTGAAAAGTTATTACAAGCAGAAAAAGTGACGAAAATTCCAGTGAAAGATTATTTAGCT
GCGACATCAGTTGGAATTTTGGAAGAACAAGGCGTTGTTCTGGATTTGAATTACGCAGAAGATTCGAAAGCAGATGTTGA
TATGAATGTAATTATGACAGGAAAAGGACATTTTGTTGAAGTGCAAGGGACTGGCGAAGAAGCGACATTTAGCCGCGCTC
AATTAAATGAATTACTTGATGCTGCAGAACATGGAATTTTACAGCTTATTGAGAAACAAAAAGAAGCATTAGGTGATATC
GTATCTCATATTGAATAG

Upstream 100 bases:

>100_bases
GGGGAATGTCTTTTTTGAATTCCCCTTCTTCTATTGTTATACATATCCTACATTTAAATTTGGCTATACTAACAAATAGT
AGTTATGAGGGGGTTATTTT

Downstream 100 bases:

>100_bases
GGGTGGAAGTATGAAACAGGTTGTAATTGCAACGAAAAATATGGGGAAAGTGCGCGAATTTGCTGAGCTATTTGAGAGAT
TTGATTTAGAAGTAAAATCT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRVDGREVRELRHVHIHTNYLKHPEGSVLIEVGDTKVICSATVEERVPPFMRGEGKGWVTAEYAMIPRATEQRTIRESSK
GKVTGRTMEIQRLIGRALRAVVDLEVLGERTVWIDCDVIQADGGTRTASITGAYVAMVLAFEKLLQAEKVTKIPVKDYLA
ATSVGILEEQGVVLDLNYAEDSKADVDMNVIMTGKGHFVEVQGTGEEATFSRAQLNELLDAAEHGILQLIEKQKEALGDI
VSHIE

Sequences:

>Translated_245_residues
MRVDGREVRELRHVHIHTNYLKHPEGSVLIEVGDTKVICSATVEERVPPFMRGEGKGWVTAEYAMIPRATEQRTIRESSK
GKVTGRTMEIQRLIGRALRAVVDLEVLGERTVWIDCDVIQADGGTRTASITGAYVAMVLAFEKLLQAEKVTKIPVKDYLA
ATSVGILEEQGVVLDLNYAEDSKADVDMNVIMTGKGHFVEVQGTGEEATFSRAQLNELLDAAEHGILQLIEKQKEALGDI
VSHIE
>Mature_245_residues
MRVDGREVRELRHVHIHTNYLKHPEGSVLIEVGDTKVICSATVEERVPPFMRGEGKGWVTAEYAMIPRATEQRTIRESSK
GKVTGRTMEIQRLIGRALRAVVDLEVLGERTVWIDCDVIQADGGTRTASITGAYVAMVLAFEKLLQAEKVTKIPVKDYLA
ATSVGILEEQGVVLDLNYAEDSKADVDMNVIMTGKGHFVEVQGTGEEATFSRAQLNELLDAAEHGILQLIEKQKEALGDI
VSHIE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=58.2938388625592, Blast_Score=251, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI71981632, Length=182, Percent_Identity=25.2747252747253, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27050; Mature: 27050

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDGREVRELRHVHIHTNYLKHPEGSVLIEVGDTKVICSATVEERVPPFMRGEGKGWVT
CCCCHHHHHHHHHEEEEECEEECCCCCEEEEECCCEEEEECCHHHHCCCCCCCCCCCEEE
AEYAMIPRATEQRTIRESSKGKVTGRTMEIQRLIGRALRAVVDLEVLGERTVWIDCDVIQ
EEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAYVAMVLAFEKLLQAEKVTKIPVKDYLAATSVGILEEQGVVLDLNYAE
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCEEEEEECCC
DSKADVDMNVIMTGKGHFVEVQGTGEEATFSRAQLNELLDAAEHGILQLIEKQKEALGDI
CCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSHIE
HHHCC
>Mature Secondary Structure
MRVDGREVRELRHVHIHTNYLKHPEGSVLIEVGDTKVICSATVEERVPPFMRGEGKGWVT
CCCCHHHHHHHHHEEEEECEEECCCCCEEEEECCCEEEEECCHHHHCCCCCCCCCCCEEE
AEYAMIPRATEQRTIRESSKGKVTGRTMEIQRLIGRALRAVVDLEVLGERTVWIDCDVIQ
EEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAYVAMVLAFEKLLQAEKVTKIPVKDYLAATSVGILEEQGVVLDLNYAE
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCEEEEEECCC
DSKADVDMNVIMTGKGHFVEVQGTGEEATFSRAQLNELLDAAEHGILQLIEKQKEALGDI
CCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSHIE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA