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The map label for this gene is sfp [H]

Identifier: 152976892

GI number: 152976892

Start: 3245067

End: 3245783

Strand: Reverse

Name: sfp [H]

Synonym: Bcer98_3191

Alternate gene names: 152976892

Gene position: 3245783-3245067 (Counterclockwise)

Preceding gene: 152976893

Following gene: 152976891

Centisome position: 79.42

GC content: 31.94

Gene sequence:

>717_bases
TTGAAACTTTCTAAATTAATAGCCGTTCAATTAACTGAAGATGTTCCTATGATAGATTTAGAGATTATTTTGTCTTTTGT
AAATCAACAAAAGCGCAGTAAATTGAAACAATATAGAAATATGAAAGATGTTCGCAGATCTCTTGTAGCGGAGTTAATTA
TTAGATTAGAAGTTCTGAAACAAGTAGAAATGAATAATGATGAGATTATATTTTTAAATAATGCCTATGGAAAACCGTTT
TTACAAGGCCTAGATTTTTTCCATTTTAATATTTCCCATGCTGGTGAATGGGTGGTATGTGCATTTGATGGTATGTCTAT
CGGAGTGGATATTGAAAAAGTTGAGCGGATTGATCTTGATATAGCAAAACATGTTTTCTCGAAAAAGGAATACGAAAATT
TGATGTTACAAAATGAACAGAAACAATTAGAGTGTTTTTTTGAATATTGGACAGCAAAAGAAAGTTATATAAAGGCTATT
GGAAAGGGACTGTCTATTCCTTTAAACTCTTTTACAATTGATTTTGAACGGGCGAGTATTATTGCTGAGGAAACAGAATC
AAAAGATTGGATGCTGAAGTCGTATGAACTCGACCTACAGTATAAGGTGACACTATGTACGAAAAATGCGTCATTTCCTG
AAGATATAATGATACGAGAGGAAAAACAAGTGTATGATGAAGTGTTATCATACGTGATGAATCGCATAACAAAGTAA

Upstream 100 bases:

>100_bases
AATACTTCTTATCATAGTTTCCCTCTTATGAAGCCTTATCAGAATGCTGTTTGTGTTGAACAAATAAAAGAGTGGATCCG
ATAGTAGGGAGGAGTTACTG

Downstream 100 bases:

>100_bases
GTATACATAAAATGCTTGTAAACAAATGAAGGGTAGCAGTTTCCCTTTTTTATCATTTCTATCGGTTACATGATGAAATC
TTACACTTCTGTAAGGTAAG

Product: 4'-phosphopantetheinyl transferase

Products: NA

Alternate protein names: Surfactin synthase-activating enzyme [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKLSKLIAVQLTEDVPMIDLEIILSFVNQQKRSKLKQYRNMKDVRRSLVAELIIRLEVLKQVEMNNDEIIFLNNAYGKPF
LQGLDFFHFNISHAGEWVVCAFDGMSIGVDIEKVERIDLDIAKHVFSKKEYENLMLQNEQKQLECFFEYWTAKESYIKAI
GKGLSIPLNSFTIDFERASIIAEETESKDWMLKSYELDLQYKVTLCTKNASFPEDIMIREEKQVYDEVLSYVMNRITK

Sequences:

>Translated_238_residues
MKLSKLIAVQLTEDVPMIDLEIILSFVNQQKRSKLKQYRNMKDVRRSLVAELIIRLEVLKQVEMNNDEIIFLNNAYGKPF
LQGLDFFHFNISHAGEWVVCAFDGMSIGVDIEKVERIDLDIAKHVFSKKEYENLMLQNEQKQLECFFEYWTAKESYIKAI
GKGLSIPLNSFTIDFERASIIAEETESKDWMLKSYELDLQYKVTLCTKNASFPEDIMIREEKQVYDEVLSYVMNRITK
>Mature_238_residues
MKLSKLIAVQLTEDVPMIDLEIILSFVNQQKRSKLKQYRNMKDVRRSLVAELIIRLEVLKQVEMNNDEIIFLNNAYGKPF
LQGLDFFHFNISHAGEWVVCAFDGMSIGVDIEKVERIDLDIAKHVFSKKEYENLMLQNEQKQLECFFEYWTAKESYIKAI
GKGLSIPLNSFTIDFERASIIAEETESKDWMLKSYELDLQYKVTLCTKNASFPEDIMIREEKQVYDEVLSYVMNRITK

Specific function: Activates the seven peptidyl carrier protein (PCP) domains of surfactin synthase SRF1/2/3 by transferring the 4'- phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for cells of B.subtilis to become producers of the lipopeptide a

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Homo sapiens, GI20357568, Length=162, Percent_Identity=29.6296296296296, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24663173, Length=257, Percent_Identity=22.9571984435798, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR004568 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 27950; Mature: 27950

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSKLIAVQLTEDVPMIDLEIILSFVNQQKRSKLKQYRNMKDVRRSLVAELIIRLEVLK
CCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
QVEMNNDEIIFLNNAYGKPFLQGLDFFHFNISHAGEWVVCAFDGMSIGVDIEKVERIDLD
HHHCCCCCEEEEECCCCCHHHHCCCCEEEEECCCCCEEEEEECCCEECCCHHHHHHHHHH
IAKHVFSKKEYENLMLQNEQKQLECFFEYWTAKESYIKAIGKGLSIPLNSFTIDFERASI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEHHHHHH
IAEETESKDWMLKSYELDLQYKVTLCTKNASFPEDIMIREEKQVYDEVLSYVMNRITK
HHHCCCCCCCEEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLSKLIAVQLTEDVPMIDLEIILSFVNQQKRSKLKQYRNMKDVRRSLVAELIIRLEVLK
CCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
QVEMNNDEIIFLNNAYGKPFLQGLDFFHFNISHAGEWVVCAFDGMSIGVDIEKVERIDLD
HHHCCCCCEEEEECCCCCHHHHCCCCEEEEECCCCCEEEEEECCCEECCCHHHHHHHHHH
IAKHVFSKKEYENLMLQNEQKQLECFFEYWTAKESYIKAIGKGLSIPLNSFTIDFERASI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEHHHHHH
IAEETESKDWMLKSYELDLQYKVTLCTKNASFPEDIMIREEKQVYDEVLSYVMNRITK
HHHCCCCCCCEEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1557038; 8407792; 8355609; 8639027; 9384377; 8939709; 9484229; 10581256 [H]