Click here to switch to the map view.

The map label for this gene is greA [H]

Identifier: 152976794

GI number: 152976794

Start: 3134159

End: 3134635

Strand: Reverse

Name: greA [H]

Synonym: Bcer98_3092

Alternate gene names: 152976794

Gene position: 3134635-3134159 (Counterclockwise)

Preceding gene: 152976795

Following gene: 152976793

Centisome position: 76.7

GC content: 37.95

Gene sequence:

>477_bases
ATGGCAACAGAAAAAACATACCCTATGACACAAGAGGGTAAAGAAAAATTAGAAAACGAACTAGAACAATTAAAAACAGT
AAGACGTAAAGAAGTTGTAGAACGTATTAAAATTGCACGTAGCTTCGGGGACCTTTCTGAGAACTCTGAGTACGATGCAG
CGAAAGATGAACAAGCATTCGTAGAAGGTCGTATCACGCAGTTAGAAAACATGATTCGTAATGCAGTAATCATTGAGGAT
ACTGGCGAAGAATCTACAGTTGTTACATTGGGGAAAACAGTAACATTTAAAGAACTTCCAGACGGAGATGAAGAAGCTTA
CACAATCGTTGGTAGTGCAGAGGCTGATCCATTTGAAGGAAGAATTTCTAACGATTCTCCAATTGCAAAAAGCCTATTAG
GTAAGCAAATTGGTGAGAAAGTAGTCATCCAAACACCAGGCGGAGAAATGCAAGTTGAAATTATCTCTGTAAAATAA

Upstream 100 bases:

>100_bases
ATATGTACTAGAAACCTCCGCTATTGGAAATGCGGAGGAGTTTTCATATTTGAACTCCTCTTTTCTCGGGGGATTGGTAT
ATAAAAGGAGTGGAAAAAGA

Downstream 100 bases:

>100_bases
TAAAAAAAGTTCACTCATAGGAGTGAACTTTTTTTATTTTCACTGAGAAGCTCCTTCTCTAAACGAAGGAAAGGCAGATT
ATAAGTGAAAATTGCTATAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MATEKTYPMTQEGKEKLENELEQLKTVRRKEVVERIKIARSFGDLSENSEYDAAKDEQAFVEGRITQLENMIRNAVIIED
TGEESTVVTLGKTVTFKELPDGDEEAYTIVGSAEADPFEGRISNDSPIAKSLLGKQIGEKVVIQTPGGEMQVEIISVK

Sequences:

>Translated_158_residues
MATEKTYPMTQEGKEKLENELEQLKTVRRKEVVERIKIARSFGDLSENSEYDAAKDEQAFVEGRITQLENMIRNAVIIED
TGEESTVVTLGKTVTFKELPDGDEEAYTIVGSAEADPFEGRISNDSPIAKSLLGKQIGEKVVIQTPGGEMQVEIISVK
>Mature_157_residues
ATEKTYPMTQEGKEKLENELEQLKTVRRKEVVERIKIARSFGDLSENSEYDAAKDEQAFVEGRITQLENMIRNAVIIEDT
GEESTVVTLGKTVTFKELPDGDEEAYTIVGSAEADPFEGRISNDSPIAKSLLGKQIGEKVVIQTPGGEMQVEIISVK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=46.7532467532467, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=33.9869281045752, Blast_Score=80, Evalue=7e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17524; Mature: 17393

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEKTYPMTQEGKEKLENELEQLKTVRRKEVVERIKIARSFGDLSENSEYDAAKDEQAF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VEGRITQLENMIRNAVIIEDTGEESTVVTLGKTVTFKELPDGDEEAYTIVGSAEADPFEG
HHHHHHHHHHHHHCEEEEECCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCCCCC
RISNDSPIAKSLLGKQIGEKVVIQTPGGEMQVEIISVK
CCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure 
ATEKTYPMTQEGKEKLENELEQLKTVRRKEVVERIKIARSFGDLSENSEYDAAKDEQAF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VEGRITQLENMIRNAVIIEDTGEESTVVTLGKTVTFKELPDGDEEAYTIVGSAEADPFEG
HHHHHHHHHHHHHCEEEEECCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCCCCC
RISNDSPIAKSLLGKQIGEKVVIQTPGGEMQVEIISVK
CCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA