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The map label for this gene is appA [H]

Identifier: 152974063

GI number: 152974063

Start: 230298

End: 232025

Strand: Direct

Name: appA [H]

Synonym: Bcer98_0219

Alternate gene names: 152974063

Gene position: 230298-232025 (Clockwise)

Preceding gene: 152974061

Following gene: 152974064

Centisome position: 5.63

GC content: 35.59

Gene sequence:

>1728_bases
ATGGAGAAAAAGTCAAAAAGATGGGCGAGTGTATTTTCGATTTTACTAGGTAGTTCACTTGTATTATCCGCTTGTGGGGG
AGGCGAAGAAGAGGTTTCAACGGAACCTGCCAAGCAGCAGGACTTGAAGAATCTCAAAGTTGAAAAAATCGCAGCTACAG
ATAAGACGAAAGTTCCGGACAAAGCAAAAAACAGAAAGGATACGCTGATTGCTGGTATTTCGAAACCAGGGGGTGTTTTC
TTACCATATTTTCAAGAGAACGGTTGGGATGGCAACGTAACATCTGTTATTTTTGCTTCCCTTGTTACAACAGATAAACA
AGGGAAACCAGTTCCCGATTTAGCGGAAAAATGGGATGTTTCTTCCGATCAATTAACATACACATTCCACTTGCGTAAAG
ATGCAAAGTTTAGTGATGGTTCACCGTTAACAGCGGATGATGTAGCATTCACATTAACGCTTTTACATGATCAAGCATAT
GAAGGTAGCGATTCTATTTTGCAATATGCGATTAAGGGGGGAAAAGATTACAGAGAAGGGAAGGCAAATGCTATTGAAGG
TATTCAAATTATTGACCCACAAACAATTACAATTACAACAGAGAAAGTAAATTCTCAAGCTTTACTCGCTTTAGGAGGAC
CAGTATTATCGAAGGCATATTACGGTAAGGACTATAAACAAAATACAAGTTTAGATTATTTAAAAGAGCTGTATGGTAAA
CCGCTTGCTGCCGGCCCTTATAAATTTGAAAAATATATTCCTGGGCAAGAAGTTCGTTTCGTAGCGAATGAACATTATTA
TGCAGGAGAACCAAAAATTAAAAATTTTATTTATAAAATTACGTCAGCAGATACGAAATTTCAACTATTCCAAACAGGTG
AGATAGACTATACAGGTGTTGGAACAGGTGATGAAATTTTAGAACAAGTAAAAGGCTTAGGATTTGCCAATATTGAAATT
GAGACGGCATCTTCATTCTCTTATATAAAAATAAATAGTAAAAAGCCATATTTAAAAGATAAAAAAGTTCGACAAGCACT
TATCTATGGATTAGATCGTAAAAAGTATGTGGATACAGCCTTGAAAGGTTACGGTACGGTAGCAAACGTACCAATTCATC
CAACTTCGTGGGCTTATACAGAAGATGGGATTAATAAGTATGAATATGACAAAGAAAAGGCGAAGAAATTATTAGATGAG
GCGGGATGGAAAGTCGGTTCGGATGGAATTCGGGAAAAAGACGGTCAAAAATTAAAAGTAACATATTATGGTGGAAGTTC
AGCAAAAGAGGGCGATCTATTCATCCCGATTGCGAAAGAGAATTATAAAGAAATAGGAATTGAGTTTAACCCAGAGTTTA
TGGATTTTAATACGATGCTTTCAAAAGTGAACAAGGGTGACTATGATTTAGCTGCTGTTGCAACTCCAATTACAGTAGAT
CCAAGCGAACCAGTTAGTGGTTATTCATCTGAAGGTGATGGAAACACGCTAGGATATAAAAATGAAAAAGTTGATGAGTT
AATTCAAAAAGGGCTTGAAACAATTGATATCGAGAAAAGAAAACCGATTTATAAAGAACTATATAAAGAATTAAGTGATG
ATCCACCTGTTATTCTATTAAACTACCGTAAAATGATTACAGCTTACAACGCAAATATAAAAGGAATTGATCCTGAAAAA
TATGACAGCATTAGTTCTAATTTACCTGTATTATCAATTGAAAAATAA

Upstream 100 bases:

>100_bases
GAACACTTATTTTTTAAAAGATAAAACAAATTTTTCCGATGGGTTTTATAGTTTTTTGAGGGCGATTTAAGAAATACTTA
AGAAAGTTAAGGTGGGGACA

Downstream 100 bases:

>100_bases
AGATAAGTCCGTATAAATAGAAGACATTTTTTTATTCGAAACGCTGAATGAAAAAATGTTCTTCTATTTTAAGTACATAA
GATTGGGGAGACGAAGGGTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MEKKSKRWASVFSILLGSSLVLSACGGGEEEVSTEPAKQQDLKNLKVEKIAATDKTKVPDKAKNRKDTLIAGISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQGKPVPDLAEKWDVSSDQLTYTFHLRKDAKFSDGSPLTADDVAFTLTLLHDQAY
EGSDSILQYAIKGGKDYREGKANAIEGIQIIDPQTITITTEKVNSQALLALGGPVLSKAYYGKDYKQNTSLDYLKELYGK
PLAAGPYKFEKYIPGQEVRFVANEHYYAGEPKIKNFIYKITSADTKFQLFQTGEIDYTGVGTGDEILEQVKGLGFANIEI
ETASSFSYIKINSKKPYLKDKKVRQALIYGLDRKKYVDTALKGYGTVANVPIHPTSWAYTEDGINKYEYDKEKAKKLLDE
AGWKVGSDGIREKDGQKLKVTYYGGSSAKEGDLFIPIAKENYKEIGIEFNPEFMDFNTMLSKVNKGDYDLAAVATPITVD
PSEPVSGYSSEGDGNTLGYKNEKVDELIQKGLETIDIEKRKPIYKELYKELSDDPPVILLNYRKMITAYNANIKGIDPEK
YDSISSNLPVLSIEK

Sequences:

>Translated_575_residues
MEKKSKRWASVFSILLGSSLVLSACGGGEEEVSTEPAKQQDLKNLKVEKIAATDKTKVPDKAKNRKDTLIAGISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQGKPVPDLAEKWDVSSDQLTYTFHLRKDAKFSDGSPLTADDVAFTLTLLHDQAY
EGSDSILQYAIKGGKDYREGKANAIEGIQIIDPQTITITTEKVNSQALLALGGPVLSKAYYGKDYKQNTSLDYLKELYGK
PLAAGPYKFEKYIPGQEVRFVANEHYYAGEPKIKNFIYKITSADTKFQLFQTGEIDYTGVGTGDEILEQVKGLGFANIEI
ETASSFSYIKINSKKPYLKDKKVRQALIYGLDRKKYVDTALKGYGTVANVPIHPTSWAYTEDGINKYEYDKEKAKKLLDE
AGWKVGSDGIREKDGQKLKVTYYGGSSAKEGDLFIPIAKENYKEIGIEFNPEFMDFNTMLSKVNKGDYDLAAVATPITVD
PSEPVSGYSSEGDGNTLGYKNEKVDELIQKGLETIDIEKRKPIYKELYKELSDDPPVILLNYRKMITAYNANIKGIDPEK
YDSISSNLPVLSIEK
>Mature_575_residues
MEKKSKRWASVFSILLGSSLVLSACGGGEEEVSTEPAKQQDLKNLKVEKIAATDKTKVPDKAKNRKDTLIAGISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQGKPVPDLAEKWDVSSDQLTYTFHLRKDAKFSDGSPLTADDVAFTLTLLHDQAY
EGSDSILQYAIKGGKDYREGKANAIEGIQIIDPQTITITTEKVNSQALLALGGPVLSKAYYGKDYKQNTSLDYLKELYGK
PLAAGPYKFEKYIPGQEVRFVANEHYYAGEPKIKNFIYKITSADTKFQLFQTGEIDYTGVGTGDEILEQVKGLGFANIEI
ETASSFSYIKINSKKPYLKDKKVRQALIYGLDRKKYVDTALKGYGTVANVPIHPTSWAYTEDGINKYEYDKEKAKKLLDE
AGWKVGSDGIREKDGQKLKVTYYGGSSAKEGDLFIPIAKENYKEIGIEFNPEFMDFNTMLSKVNKGDYDLAAVATPITVD
PSEPVSGYSSEGDGNTLGYKNEKVDELIQKGLETIDIEKRKPIYKELYKELSDDPPVILLNYRKMITAYNANIKGIDPEK
YDSISSNLPVLSIEK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=472, Percent_Identity=26.271186440678, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1787495, Length=491, Percent_Identity=25.2545824847251, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789887, Length=495, Percent_Identity=24.2424242424242, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787762, Length=514, Percent_Identity=26.6536964980545, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787052, Length=503, Percent_Identity=24.8508946322068, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1787551, Length=474, Percent_Identity=22.3628691983122, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789397, Length=481, Percent_Identity=23.7006237006237, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081878, Length=472, Percent_Identity=23.0932203389831, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63878; Mature: 63878

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKSKRWASVFSILLGSSLVLSACGGGEEEVSTEPAKQQDLKNLKVEKIAATDKTKVPD
CCCHHHHHHHHHHHHHCCHHEEEECCCCCHHCCCCCHHHHHHHCCCEEEEECCCCCCCCC
KAKNRKDTLIAGISKPGGVFLPYFQENGWDGNVTSVIFASLVTTDKQGKPVPDLAEKWDV
HHCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCC
SSDQLTYTFHLRKDAKFSDGSPLTADDVAFTLTLLHDQAYEGSDSILQYAIKGGKDYREG
CCCCEEEEEEEECCCCCCCCCCCCHHHHEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
KANAIEGIQIIDPQTITITTEKVNSQALLALGGPVLSKAYYGKDYKQNTSLDYLKELYGK
CCCCCCCEEEECCCEEEEEECCCCCEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHCC
PLAAGPYKFEKYIPGQEVRFVANEHYYAGEPKIKNFIYKITSADTKFQLFQTGEIDYTGV
CCCCCCCHHHHCCCCCCEEEEECCCEECCCCHHHHHEEEEECCCCEEEEEECCCEEEECC
GTGDEILEQVKGLGFANIEIETASSFSYIKINSKKPYLKDKKVRQALIYGLDRKKYVDTA
CCHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LKGYGTVANVPIHPTSWAYTEDGINKYEYDKEKAKKLLDEAGWKVGSDGIREKDGQKLKV
HHCCCCEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCEEEE
TYYGGSSAKEGDLFIPIAKENYKEIGIEFNPEFMDFNTMLSKVNKGDYDLAAVATPITVD
EEECCCCCCCCCEEEEECCCCHHHCCCEECCCEECHHHHHHHCCCCCCCEEEEEECEEEC
PSEPVSGYSSEGDGNTLGYKNEKVDELIQKGLETIDIEKRKPIYKELYKELSDDPPVILL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCEEEE
NYRKMITAYNANIKGIDPEKYDSISSNLPVLSIEK
ECCEEEEEECCCCCCCCCHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MEKKSKRWASVFSILLGSSLVLSACGGGEEEVSTEPAKQQDLKNLKVEKIAATDKTKVPD
CCCHHHHHHHHHHHHHCCHHEEEECCCCCHHCCCCCHHHHHHHCCCEEEEECCCCCCCCC
KAKNRKDTLIAGISKPGGVFLPYFQENGWDGNVTSVIFASLVTTDKQGKPVPDLAEKWDV
HHCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCC
SSDQLTYTFHLRKDAKFSDGSPLTADDVAFTLTLLHDQAYEGSDSILQYAIKGGKDYREG
CCCCEEEEEEEECCCCCCCCCCCCHHHHEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
KANAIEGIQIIDPQTITITTEKVNSQALLALGGPVLSKAYYGKDYKQNTSLDYLKELYGK
CCCCCCCEEEECCCEEEEEECCCCCEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHCC
PLAAGPYKFEKYIPGQEVRFVANEHYYAGEPKIKNFIYKITSADTKFQLFQTGEIDYTGV
CCCCCCCHHHHCCCCCCEEEEECCCEECCCCHHHHHEEEEECCCCEEEEEECCCEEEECC
GTGDEILEQVKGLGFANIEIETASSFSYIKINSKKPYLKDKKVRQALIYGLDRKKYVDTA
CCHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LKGYGTVANVPIHPTSWAYTEDGINKYEYDKEKAKKLLDEAGWKVGSDGIREKDGQKLKV
HHCCCCEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCEEEE
TYYGGSSAKEGDLFIPIAKENYKEIGIEFNPEFMDFNTMLSKVNKGDYDLAAVATPITVD
EEECCCCCCCCCEEEEECCCCHHHCCCEECCCEECHHHHHHHCCCCCCCEEEEEECEEEC
PSEPVSGYSSEGDGNTLGYKNEKVDELIQKGLETIDIEKRKPIYKELYKELSDDPPVILL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCEEEE
NYRKMITAYNANIKGIDPEKYDSISSNLPVLSIEK
ECCEEEEEECCCCCCCCCHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]