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The map label for this gene is truA [H]

Identifier: 152973988

GI number: 152973988

Start: 144567

End: 145310

Strand: Direct

Name: truA [H]

Synonym: Bcer98_0136

Alternate gene names: 152973988

Gene position: 144567-145310 (Clockwise)

Preceding gene: 152973987

Following gene: 152973989

Centisome position: 3.54

GC content: 36.83

Gene sequence:

>744_bases
ATGGAAAGAATAAAGTGTACAGTTGCATATGATGGGATTAACTTTTGTGGATATCAAATCCAACCGCAGCATCGTACTGT
GCAACAAGAAATTGAAAAGGCTTTGCAAAAGTTGCACAAAGGAGAACTTGTTCGTGTACAGGCGTCAGGAAGAACAGACT
CTACTGTTCATGCAAAAGGACAAGTAATTCATTTTGATACACCATTGTCTCTTGAAGAATGGCAGTGGGGGAACGCTTTA
AATACATTATTGCCAGACGATATTGTAATTGTACAAGTAGAGAAGAAAACGGAAGAGTTCCATGCGCGTTATGGCGTTGT
AAAAAAGGAATATCGTTACCGTGTATTATTATCTAAAACGGCGGATGTATTCCGTAGAAATTATGTATATCAGTATCCAT
ATCCAATTGATATTCAATTGATAAGAAAAGCTATCCCTTATTTTATCGGGACACATGATTTCACATCATTTTGTTCGGCG
AAGACTGATAAAAAAGATAAAGTGCGAACAATTTATGAGATAGATTTATTCGAACAAGGTGATGAATTGGTTTTTCGCTT
TGTTGGTAATGGTTTTTTATATAATATGGTTAGAATCATTGTCGGAACGCTACTTAGCGTAGGGCAAGGAAAGGTTGATC
CAGATAGTATTCCAGAGATTTTAGCTAAGCAAGATCGACGCTTTGCGGGAAAGATGGTACCAGGTCATGGTCTTTATTTA
TGGCAGGTAAACTATAACAACTAA

Upstream 100 bases:

>100_bases
GGCAACTACGCTGGAAAACAAGTGATACTGTAACATTAATAAGCTTATTTTGCTTGGCGTGTATTCTAGTATGGGTAAGA
TCATAATGGAGAGTAGAAAG

Downstream 100 bases:

>100_bases
TCCTGGTGTAACATTTGCTTGACATTAGATTCTCAAAACGATATCATAACATATGGTATTGTTTCTAAAACCACGATTAG
CCCCGGAAGGAAGTCGTGTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1 [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MERIKCTVAYDGINFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQWGNAL
NTLLPDDIVIVQVEKKTEEFHARYGVVKKEYRYRVLLSKTADVFRRNYVYQYPYPIDIQLIRKAIPYFIGTHDFTSFCSA
KTDKKDKVRTIYEIDLFEQGDELVFRFVGNGFLYNMVRIIVGTLLSVGQGKVDPDSIPEILAKQDRRFAGKMVPGHGLYL
WQVNYNN

Sequences:

>Translated_247_residues
MERIKCTVAYDGINFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQWGNAL
NTLLPDDIVIVQVEKKTEEFHARYGVVKKEYRYRVLLSKTADVFRRNYVYQYPYPIDIQLIRKAIPYFIGTHDFTSFCSA
KTDKKDKVRTIYEIDLFEQGDELVFRFVGNGFLYNMVRIIVGTLLSVGQGKVDPDSIPEILAKQDRRFAGKMVPGHGLYL
WQVNYNN
>Mature_247_residues
MERIKCTVAYDGINFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQWGNAL
NTLLPDDIVIVQVEKKTEEFHARYGVVKKEYRYRVLLSKTADVFRRNYVYQYPYPIDIQLIRKAIPYFIGTHDFTSFCSA
KTDKKDKVRTIYEIDLFEQGDELVFRFVGNGFLYNMVRIIVGTLLSVGQGKVDPDSIPEILAKQDRRFAGKMVPGHGLYL
WQVNYNN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=249, Percent_Identity=30.5220883534137, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=32.9218106995885, Blast_Score=148, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321112, Length=239, Percent_Identity=27.6150627615063, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116007752, Length=306, Percent_Identity=24.8366013071895, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281362146, Length=263, Percent_Identity=24.3346007604563, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648455, Length=263, Percent_Identity=24.7148288973384, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28655; Mature: 28655

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIKCTVAYDGINFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKG
CCCEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCC
QVIHFDTPLSLEEWQWGNALNTLLPDDIVIVQVEKKTEEFHARYGVVKKEYRYRVLLSKT
EEEEECCCCCHHHCCCCCHHHHCCCCCEEEEEEECHHHHHHHHHCCHHHHHHEEEEHHHH
ADVFRRNYVYQYPYPIDIQLIRKAIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIDLFEQG
HHHHHHCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEECHHCC
DELVFRFVGNGFLYNMVRIIVGTLLSVGQGKVDPDSIPEILAKQDRRFAGKMVPGHGLYL
CCEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
WQVNYNN
EEEECCC
>Mature Secondary Structure
MERIKCTVAYDGINFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKG
CCCEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCC
QVIHFDTPLSLEEWQWGNALNTLLPDDIVIVQVEKKTEEFHARYGVVKKEYRYRVLLSKT
EEEEECCCCCHHHCCCCCHHHHCCCCCEEEEEEECHHHHHHHHHCCHHHHHHEEEEHHHH
ADVFRRNYVYQYPYPIDIQLIRKAIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIDLFEQG
HHHHHHCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEECHHCC
DELVFRFVGNGFLYNMVRIIVGTLLSVGQGKVDPDSIPEILAKQDRRFAGKMVPGHGLYL
CCEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
WQVNYNN
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]