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The map label for this gene is tuf [H]

Identifier: 152973954

GI number: 152973954

Start: 125691

End: 126878

Strand: Direct

Name: tuf [H]

Synonym: Bcer98_0102

Alternate gene names: 152973954

Gene position: 125691-126878 (Clockwise)

Preceding gene: 152973953

Following gene: 152973955

Centisome position: 3.08

GC content: 44.19

Gene sequence:

>1188_bases
ATGGCTAAAGAAAAATTCGAACGTTCAAAACCCCATGTTAACATTGGTACAATTGGCCACGTTGACCATGGTAAAACTAC
ATTAACTGCTGCAATCACTACAGTTCTTGCAAAAGCTGGTGGTGCTGAAGCACGCGGATACGATCAAATCGACGCTGCTC
CAGAAGAAAGAGAGCGCGGTATCACAATCTCAACTGCACACGTTGAGTACGAAACTGAAACTCGTCACTATGCACACGTT
GACTGCCCAGGACACGCTGACTATGTTAAAAACATGATCACTGGTGCTGCTCAAATGGACGGCGGTATCTTAGTAGTATC
TGCTGCTGATGGTCCAATGCCACAAACTCGTGAGCACATCCTTCTTTCTCGTCAAGTAGGTGTACCTTACATCGTTGTAT
TCTTAAACAAATGCGACATGGTAGACGACGAAGAATTACTAGAATTAGTAGAAATGGAAGTTCGTGATCTATTATCTGAA
TACGAATTCCCAGGAGATGACATCCCTGTAGTTAAAGGTTCTGCTCTTAAAGCTCTTGAAGGAGATGCTGAGTGGGAAGC
TAAAATCGTTGAGTTAATGGCTGAAGTTGATGCTTACATCCCAACTCCAGAACGTGAAACTGACAAACCATTCTTAATGC
CTGTAGAGGATGTATTCTCTATCACAGGTCGTGGTACAGTTGCTACTGGTCGCGTAGAGCGCGGTGTAGTTAAAGTTGGT
GACGTAGTAGAAATCATCGGTCTTGCTGAAGAAACTGCTTCTACAACTGTAACAGGTGTAGAAATGTTCCGTAAGCTTCT
TGACCAAGCTGAAGCTGGTGACAACATCGGTGCTCTACTTCGTGGGGTTGCTCGTGAAGACATCCAACGTGGACAAGTTC
TTGCTAAACCAGGCTCTGTAAAAGCTCACGCTAAATTCAAAGCTGAAGTTTACGTTTTATCTAAAGAAGAAGGTGGACGT
CACACTCCATTCTTCGCTAACTACCGCCCACAATTCTATTTCCGTACAACTGACGTAACTGGTATCATCCAATTACCAGA
AGGTACTGAAATGGTAATGCCTGGCGACAACGTTGAAATGACTGTTGAACTTATCGCTCCTGTAGCAGTAGAAGAGGGAA
CTAAGTTCTCTATCCGTGAAGGCGGACGTACTGTAGGTGCTGGTGTAGTTGCTTCTATCATCGAATAG

Upstream 100 bases:

>100_bases
TCAAGTATAACTACTTATGTAAGCTTAGAAAGTGAGGCTCTAATTCTCACTTTCTAGCCTAAAATATAAATTTGAAGATA
CCATTAAGGAGGATTTTAGA

Downstream 100 bases:

>100_bases
TCTCAACTATAAATAATAAAAACCCAAGGGGGATTTCCCTTGGGTTTTTTTGCTGTATAAACAGGAGGTAAATGTAGATT
GTGACGAGTTTCTTCTATAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERGITISTAHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSE
YEFPGDDIPVVKGSALKALEGDAEWEAKIVELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGVVKVG
DVVEIIGLAEETASTTVTGVEMFRKLLDQAEAGDNIGALLRGVAREDIQRGQVLAKPGSVKAHAKFKAEVYVLSKEEGGR
HTPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNVEMTVELIAPVAVEEGTKFSIREGGRTVGAGVVASIIE

Sequences:

>Translated_395_residues
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERGITISTAHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSE
YEFPGDDIPVVKGSALKALEGDAEWEAKIVELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGVVKVG
DVVEIIGLAEETASTTVTGVEMFRKLLDQAEAGDNIGALLRGVAREDIQRGQVLAKPGSVKAHAKFKAEVYVLSKEEGGR
HTPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNVEMTVELIAPVAVEEGTKFSIREGGRTVGAGVVASIIE
>Mature_394_residues
AKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERGITISTAHVEYETETRHYAHVD
CPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEY
EFPGDDIPVVKGSALKALEGDAEWEAKIVELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGVVKVGD
VVEIIGLAEETASTTVTGVEMFRKLLDQAEAGDNIGALLRGVAREDIQRGQVLAKPGSVKAHAKFKAEVYVLSKEEGGRH
TPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNVEMTVELIAPVAVEEGTKFSIREGGRTVGAGVVASIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.4683544303797, Blast_Score=477, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=442, Percent_Identity=29.4117647058824, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=442, Percent_Identity=29.4117647058824, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI4503475, Length=452, Percent_Identity=29.4247787610619, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=29.5555555555556, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=28.9719626168224, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194018522, Length=346, Percent_Identity=27.4566473988439, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI194018520, Length=345, Percent_Identity=27.536231884058, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=345, Percent_Identity=27.536231884058, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI46094014, Length=331, Percent_Identity=27.7945619335347, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI4503507, Length=337, Percent_Identity=27.299703264095, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI157426893, Length=251, Percent_Identity=27.0916334661355, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=77.1573604060914, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=77.1573604060914, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI2367247, Length=336, Percent_Identity=30.6547619047619, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI48994988, Length=272, Percent_Identity=33.8235294117647, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789108, Length=323, Percent_Identity=30.6501547987616, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=31.5068493150685, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788922, Length=147, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=52.020202020202, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=43.5233160621762, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=452, Percent_Identity=31.4159292035398, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17569207, Length=452, Percent_Identity=31.4159292035398, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=29.7727272727273, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=457, Percent_Identity=26.9146608315098, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=431, Percent_Identity=26.9141531322506, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=428, Percent_Identity=26.8691588785047, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=267, Percent_Identity=29.5880149812734, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=157, Percent_Identity=37.5796178343949, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=291, Percent_Identity=26.1168384879725, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=30.1724137931034, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=534, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=31.0810810810811, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=31.0810810810811, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=349, Percent_Identity=25.5014326647564, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=453, Percent_Identity=26.9315673289183, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=267, Percent_Identity=28.4644194756554, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=59.6446700507614, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=59.6446700507614, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=48.0519480519481, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=30.4157549234136, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=30.4157549234136, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=30.4157549234136, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=30.4157549234136, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24652838, Length=457, Percent_Identity=30.1969365426696, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI17137572, Length=457, Percent_Identity=30.1969365426696, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=32.6732673267327, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45550900, Length=350, Percent_Identity=29.4285714285714, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=28.6127167630058, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24647048, Length=356, Percent_Identity=25.2808988764045, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24647046, Length=356, Percent_Identity=25.2808988764045, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28572034, Length=238, Percent_Identity=34.0336134453782, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582462, Length=162, Percent_Identity=32.7160493827161, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI78706572, Length=236, Percent_Identity=27.5423728813559, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42894; Mature: 42763

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
ITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
LLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYEFPGDDIPVVKGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GDAEWEAKIVELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGVVKVG
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHCCCCEEEECCHHCCEEECC
DVVEIIGLAEETASTTVTGVEMFRKLLDQAEAGDNIGALLRGVAREDIQRGQVLAKPGSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCE
KAHAKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNVEM
EEEEEEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
TVELIAPVAVEEGTKFSIREGGRTVGAGVVASIIE
EEEEECCHHHCCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
ITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
LLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYEFPGDDIPVVKGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GDAEWEAKIVELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGVVKVG
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHCCCCEEEECCHHCCEEECC
DVVEIIGLAEETASTTVTGVEMFRKLLDQAEAGDNIGALLRGVAREDIQRGQVLAKPGSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCE
KAHAKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNVEM
EEEEEEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
TVELIAPVAVEEGTKFSIREGGRTVGAGVVASIIE
EEEEECCHHHCCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA