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The map label for this gene is lysS [H]

Identifier: 152973925

GI number: 152973925

Start: 80869

End: 82368

Strand: Direct

Name: lysS [H]

Synonym: Bcer98_0072

Alternate gene names: 152973925

Gene position: 80869-82368 (Clockwise)

Preceding gene: 152973924

Following gene: 152973926

Centisome position: 1.98

GC content: 36.53

Gene sequence:

>1500_bases
ATGGATAACATGAACCATGAAGAATTAAACGACCAATTACTTGTTCGTCGTGAGAAACTACATAACTTACGTGAACAAGG
TATCGATCCATTTGGTAAACGTTTTGAACGTACACATTCATCAAAAGAGTTACTGAACTTGTATGGAGAGTTCTCTAAAG
AAGAATTAGAGGAAAAAGCAATTTCTGTTTCTATTGCTGGGCGCATTATGACAAAGCGCGGTAAAGGGAAAGCTGGTTTT
GCGCATATTCAAGATTTACATGGACAAGTTCAAATTTACGTTCGTAAGGATGCCGTTGGAGATGAAGAATATGAATTGTT
TAAAACGGCTGATTTAGGTGACTTAGTCGGAATTGAAGGGAAAGTTTTCAAAACAAATGTTGGAGAACTTTCTGTAAAAG
CAACATCATTTACTTTATTAACGAAATCTCTTCGTCCACTTCCGGATAAATACCATGGATTGAAAGATATTGAGCAACGT
TATCGTCAACGTTACTTAGATCTAATCACAAGTATGGAGAGTCGCGAAACATTTGTTACTCGCAGTAAAATCATCCGTGA
AATGAGAAGATATCTGGATGATAATGGCTATCTTGAGGTAGAAACACCAATGATGCATACAATTGCAGGTGGTGCATCTG
CTCGTCCGTTCATTACGCATCATAATGCATTAGATATGGAATTATATATGCGTATTGCAATTGAGTTGCATTTAAAACGT
CTTATTGTAGGCGGCCTAGAAAAAGTATATGAAATTGGCCGTGTTTTCCGTAATGAAGGTGTATCAACACGTCATAATCC
AGAATTTACAATGATTGAATTATATGAAGCGTATGCTGACTATAAAGATATTATGAAACTAACAGAAAATATGATTGCTC
ATATTGCGAAGCGAGTGTTGGGTACAACAACAATCCAATATGGTGAACATGAAATTAATTTAGAACCAGAATGGACGCGT
CTTCATATGGTAGATGCAATTAAACAATATTGTGGAGTGGACTTCTGGAAACCAATGAGCGTGGAAGAAGCGCGTGCATT
AGCGAAAGAACATGATGTAGAAATTAAAGATACAATGGAAGTTGGTCACATCATTAACGAATTCTTTGAACAAAAAGTAG
AAGAAAAGTTAATTCAACCTACATTTATCTACGGGCATCCAGTAGAAATTTCGCCTCTTGCGAAAAAGAACGATGAGGAT
TCACGATTTACAGATCGTTTTGAACTATTCATTGTTGCACGCGAACATGCAAATGCATTTACTGAATTAAATGATCCAAT
CGATCAGAAAGAACGTTTTGAAGCTCAATTAAAAGAGCGTGAACAAGGAAATGATGAGGCGCATATGATGGACGATGATT
ATATCGAAGCTCTTGAATATGGTATGCCTCCTACTGGTGGATTAGGTATTGGTATTGATCGCCTTGTTATGCTATTAACA
AATTCACCATCTATCCGTGATGTATTGTTATTCCCTACAATGCGTCATAAACAAGACTAA

Upstream 100 bases:

>100_bases
AAGAACTGTCAGTTACTTACTGGCAGTTTTTCTAGTTGGATAGAAAATGATATACTGTATATATTGTAAAATTAAATAGA
GCTGGAGTGATATCAATACC

Downstream 100 bases:

>100_bases
GCTTTGGAGATTTCTCCAAAGCTTTTTTTAATCCTACTATTTATAGAGAGTAAGATCTCTTATCTTAAGATTTAAAAAGA
AATGAGAAAGGTATTTTTAT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTHSSKELLNLYGEFSKEELEEKAISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATSFTLLTKSLRPLPDKYHGLKDIEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHTIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMIAHIAKRVLGTTTIQYGEHEINLEPEWTR
LHMVDAIKQYCGVDFWKPMSVEEARALAKEHDVEIKDTMEVGHIINEFFEQKVEEKLIQPTFIYGHPVEISPLAKKNDED
SRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NSPSIRDVLLFPTMRHKQD

Sequences:

>Translated_499_residues
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTHSSKELLNLYGEFSKEELEEKAISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATSFTLLTKSLRPLPDKYHGLKDIEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHTIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMIAHIAKRVLGTTTIQYGEHEINLEPEWTR
LHMVDAIKQYCGVDFWKPMSVEEARALAKEHDVEIKDTMEVGHIINEFFEQKVEEKLIQPTFIYGHPVEISPLAKKNDED
SRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NSPSIRDVLLFPTMRHKQD
>Mature_499_residues
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTHSSKELLNLYGEFSKEELEEKAISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATSFTLLTKSLRPLPDKYHGLKDIEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHTIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMIAHIAKRVLGTTTIQYGEHEINLEPEWTR
LHMVDAIKQYCGVDFWKPMSVEEARALAKEHDVEIKDTMEVGHIINEFFEQKVEEKLIQPTFIYGHPVEISPLAKKNDED
SRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NSPSIRDVLLFPTMRHKQD

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=504, Percent_Identity=41.0714285714286, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI194272210, Length=504, Percent_Identity=41.0714285714286, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI40789249, Length=260, Percent_Identity=25, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1790571, Length=493, Percent_Identity=53.1440162271805, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=53.265306122449, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI87082379, Length=333, Percent_Identity=32.4324324324324, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788173, Length=248, Percent_Identity=27.4193548387097, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=491, Percent_Identity=44.6028513238289, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17535927, Length=491, Percent_Identity=44.6028513238289, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71994340, Length=491, Percent_Identity=44.6028513238289, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32566633, Length=313, Percent_Identity=23.6421725239617, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=497, Percent_Identity=44.0643863179074, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324256, Length=477, Percent_Identity=31.8658280922432, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=350, Percent_Identity=24.8571428571429, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=323, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24640849, Length=505, Percent_Identity=41.5841584158416, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640851, Length=505, Percent_Identity=41.5841584158416, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24584738, Length=276, Percent_Identity=25.3623188405797, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57884; Mature: 57884

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTHSSKELLNLYGEFSKEELEEKA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ISVSIAGRIMTKRGKGKAGFAHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEG
HHHEEHHHHHCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCCCHHCCCCC
KVFKTNVGELSVKATSFTLLTKSLRPLPDKYHGLKDIEQRYRQRYLDLITSMESRETFVT
EEEEECCCCEEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSKIIREMRRYLDDNGYLEVETPMMHTIAGGASARPFITHHNALDMELYMRIAIELHLKR
HHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMIAHIAKRVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTTIQYGEHEINLEPEWTRLHMVDAIKQYCGVDFWKPMSVEEARALAKEHDVEIKDTME
HHHHEEECCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
VGHIINEFFEQKVEEKLIQPTFIYGHPVEISPLAKKNDEDSRFTDRFELFIVAREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCHHHHCEEEEEEEEEHHCCHH
TELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
NSPSIRDVLLFPTMRHKQD
CCCCCCEEEECCCHHCCCC
>Mature Secondary Structure
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTHSSKELLNLYGEFSKEELEEKA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ISVSIAGRIMTKRGKGKAGFAHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEG
HHHEEHHHHHCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCCCHHCCCCC
KVFKTNVGELSVKATSFTLLTKSLRPLPDKYHGLKDIEQRYRQRYLDLITSMESRETFVT
EEEEECCCCEEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSKIIREMRRYLDDNGYLEVETPMMHTIAGGASARPFITHHNALDMELYMRIAIELHLKR
HHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMIAHIAKRVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTTIQYGEHEINLEPEWTRLHMVDAIKQYCGVDFWKPMSVEEARALAKEHDVEIKDTME
HHHHEEECCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
VGHIINEFFEQKVEEKLIQPTFIYGHPVEISPLAKKNDEDSRFTDRFELFIVAREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCHHHHCEEEEEEEEEHHCCHH
TELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
NSPSIRDVLLFPTMRHKQD
CCCCCCEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA