Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 152973924

GI number: 152973924

Start: 79710

End: 80708

Strand: Direct

Name: dus1 [H]

Synonym: Bcer98_0071

Alternate gene names: 152973924

Gene position: 79710-80708 (Clockwise)

Preceding gene: 152973923

Following gene: 152973925

Centisome position: 1.95

GC content: 40.54

Gene sequence:

>999_bases
GTGTTAAAGATTGCAAACATTGAGATGAAGAACCCAGTGGTACTAGCACCGATGGCGGGAGTATGCAATTCTGCATTCCG
TTTAACAGTTAAGGAATTTGGTGCGGGATTGGTATGTGCTGAAATGGTAAGTGACAAGGCCATTTTATTTAATAATAAAA
GAACATTAGATATGTTATATATTGATGAGAGAGAAAAGCCGCTAAGTCTACAAATTTTTGGCGGCGAGAAAGAGACGCTT
GTTGAGGCTGCAAAATATGTAGATCAAAATACAACGGCGGATATTATTGATATTAATATGGGGTGTCCAGTTCCGAAAAT
TACAAAATGTGATGCGGGAGCAAAGTGGCTTTTAGACCCAAATAAAATATATGAAATGGTAGCAGCAGTTGTTGATGCGG
TGGAAAAACCAGTTACGGTTAAAATGCGTATTGGCTGGGATGAAGATCATATCTTCGCAATTGAAAATGCTAGAGCGGTT
GAACGTGCGGGAGGTCAAGCTGTAGCAGTTCATGGACGCACGCGTGTTCAAATGTACGAGGGTAAAGCAGATTGGGATAT
TATTAAACAAGTGAAACAAGCGGTGAATATTCCTGTTATCGGTAATGGTGATGTGGAAACTCCGCAAGATGCTAAGCGTA
TGCTAGATGAAATTGGTGTAGATGGGGTTATGATTGGGCGTGCAGCGCTTGGAAATCCGTGGATGATTTATCGTACTGTG
AAGTATTTAGAAACTGGAGAATTAATGCCAGAACCAACAGCGCGTGAGAAAGTTGATGTATGTATGCTACATTTAGATCG
TCTCATCGATTTAAAGAATGAGCATATTGCAGTACGCGAAATGAGGAAGCATGCAGCTTGGTATTTAAAAGGTGTTCGCG
GAAATGCGAGAGTGCGCAATGGGATCAATGCTTGTAATACTCGTGAAGATCTTGCTTCACTATTAGGTGCATTTGTAGAA
GAGGTAGAAGCGAAACAAGAAAATATTCATATCAGTTGA

Upstream 100 bases:

>100_bases
GATCACCTTCTCAAGATTTTGTTGTAGAAGTTGCAAAGACATTGAACATTTCAATAGATGAGTTAATGCCAAAGTAAGGA
TAATGAAAGGGGCTAGTCGA

Downstream 100 bases:

>100_bases
GAAGCGAGAGGGAATGATTGACATTCTGTCTTTTGCTTCCTATAATTACGTGAAAGAAAGAAGAACTGTCAGTTACTTAC
TGGCAGTTTTTCTAGTTGGA

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILFNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VEAAKYVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENARAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQAVNIPVIGNGDVETPQDAKRMLDEIGVDGVMIGRAALGNPWMIYRTV
KYLETGELMPEPTAREKVDVCMLHLDRLIDLKNEHIAVREMRKHAAWYLKGVRGNARVRNGINACNTREDLASLLGAFVE
EVEAKQENIHIS

Sequences:

>Translated_332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILFNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VEAAKYVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENARAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQAVNIPVIGNGDVETPQDAKRMLDEIGVDGVMIGRAALGNPWMIYRTV
KYLETGELMPEPTAREKVDVCMLHLDRLIDLKNEHIAVREMRKHAAWYLKGVRGNARVRNGINACNTREDLASLLGAFVE
EVEAKQENIHIS
>Mature_332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILFNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VEAAKYVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENARAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQAVNIPVIGNGDVETPQDAKRMLDEIGVDGVMIGRAALGNPWMIYRTV
KYLETGELMPEPTAREKVDVCMLHLDRLIDLKNEHIAVREMRKHAAWYLKGVRGNARVRNGINACNTREDLASLLGAFVE
EVEAKQENIHIS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=30.4721030042918, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=30.7359307359307, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=31.7180616740088, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI8923374, Length=247, Percent_Identity=28.7449392712551, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=30.5309734513274, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=38.6292834890966, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1788462, Length=295, Percent_Identity=34.2372881355932, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI145693211, Length=227, Percent_Identity=28.1938325991189, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=228, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=245, Percent_Identity=31.4285714285714, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=28.7449392712551, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=29.3023255813954, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=30.1724137931034, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=283, Percent_Identity=25.4416961130742, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=286, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=241, Percent_Identity=34.0248962655602, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=32.8125, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=32.8125, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45549423, Length=260, Percent_Identity=28.4615384615385, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=30.3964757709251, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36941; Mature: 36941

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILFNNKRTLDMLY
CEEEEEEECCCCEEEECHHHHCCHHHEEEHHHHCCCCEEHHHHCCCEEEECCCCEEEEEE
IDEREKPLSLQIFGGEKETLVEAAKYVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDP
EECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCEEEECH
NKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENARAVERAGGQAVAVHGRTRVQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHCCCCEEEEECCEEEEEEC
GKADWDIIKQVKQAVNIPVIGNGDVETPQDAKRMLDEIGVDGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCEEEECCHHCCCCEEEEEEH
KYLETGELMPEPTAREKVDVCMLHLDRLIDLKNEHIAVREMRKHAAWYLKGVRGNARVRN
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCHHHC
GINACNTREDLASLLGAFVEEVEAKQENIHIS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILFNNKRTLDMLY
CEEEEEEECCCCEEEECHHHHCCHHHEEEHHHHCCCCEEHHHHCCCEEEECCCCEEEEEE
IDEREKPLSLQIFGGEKETLVEAAKYVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDP
EECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCEEEECH
NKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENARAVERAGGQAVAVHGRTRVQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHCCCCEEEEECCEEEEEEC
GKADWDIIKQVKQAVNIPVIGNGDVETPQDAKRMLDEIGVDGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCEEEECCHHCCCCEEEEEEH
KYLETGELMPEPTAREKVDVCMLHLDRLIDLKNEHIAVREMRKHAAWYLKGVRGNARVRN
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCHHHC
GINACNTREDLASLLGAFVEEVEAKQENIHIS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]