Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 152969671

GI number: 152969671

Start: 1269327

End: 1270142

Strand: Direct

Name: cobB [H]

Synonym: KPN_01117

Alternate gene names: 152969671

Gene position: 1269327-1270142 (Clockwise)

Preceding gene: 152969670

Following gene: 152969672

Centisome position: 23.88

GC content: 62.13

Gene sequence:

>816_bases
ATGCAGTCGCGCCGTTTACATCGATTGAGCCGTTTTCGTCGCAATAAACGTCGGTTGCGTGACCGTCTGCGTCAGCGGAT
TTTCTTTAGGGAGGACAGGATGAAGCCTGAAGCGATGGCAAAACCGAGAGTGGTGGTGTTAACCGGCGCGGGCATCTCGG
CAGAGTCGGGGATCAAAACCTTCCGCGCGGCCGATGGCCTGTGGGAAGAGCATCGGGTGGAAGATGTGGCGACGCCGGAA
GGCTTCGCCCGCGATCCGGCGCTGGTGCAGGCGTTCTACAACGCCCGTCGTCGTCAGCTGCAGAGTCCGGAAATCGCGCC
GAACGCGGCGCATCTGGCGCTGGCGCGTCTGGAGGATCTGCTGGGCGACCATTTTCTGCTGGTCACCCAGAATATTGATA
ATCTGCACGAGCGCGCCGGCAACCGCCGGGTGATCCATATGCACGGCGAGCTGCTAAAGGTGCGCTGTTCGTGGAGCGGC
CAGGTGCTGGAGTGGACCGGCGACGTCACCGCCGAGGACAAGTGCCACTGCTGCCAGTTCCCGGCGGCGCTGCGTCCGCA
CGTGGTCTGGTTTGGCGAAATGCCGCTGGGGATGGATGAGATCTACAGCGCGCTGGCCGACGCAGATATTTTTATCGCCA
TCGGCACCTCCGGCCATGTCTATCCGGCGGCTGGCTTTGTGCACGAGGCGCGACTGCACGGCGCCCATACCGTTGAACTG
AACCTGGAGCCCAGCCAGGTGGGCAGCGAGTTTGCCGAGAAGCACTACGGGCTGGCAAGCGAAGTGGTACCCGCCTTTAT
CGATAAGCTGCTGTAG

Upstream 100 bases:

>100_bases
TCGCCAGGGTTCCGCGCATTGAGCGCGCGCGCCACGGCGATGCCGGGGGAATGCGCGGTGCGGCCTTCCTTCACCTGACC
CATTAATCACAGAGGTAGCT

Downstream 100 bases:

>100_bases
GAAAACGCGCTGTAAACTGAAGCCGCCTGCCGGTAGCCAGGGCGCAGCCCACGGCCGCCGGCAGCGCTGCTGACAAGGAT
GTCACGTCATGACAGGAGTC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MQSRRLHRLSRFRRNKRRLRDRLRQRIFFREDRMKPEAMAKPRVVVLTGAGISAESGIKTFRAADGLWEEHRVEDVATPE
GFARDPALVQAFYNARRRQLQSPEIAPNAAHLALARLEDLLGDHFLLVTQNIDNLHERAGNRRVIHMHGELLKVRCSWSG
QVLEWTGDVTAEDKCHCCQFPAALRPHVVWFGEMPLGMDEIYSALADADIFIAIGTSGHVYPAAGFVHEARLHGAHTVEL
NLEPSQVGSEFAEKHYGLASEVVPAFIDKLL

Sequences:

>Translated_271_residues
MQSRRLHRLSRFRRNKRRLRDRLRQRIFFREDRMKPEAMAKPRVVVLTGAGISAESGIKTFRAADGLWEEHRVEDVATPE
GFARDPALVQAFYNARRRQLQSPEIAPNAAHLALARLEDLLGDHFLLVTQNIDNLHERAGNRRVIHMHGELLKVRCSWSG
QVLEWTGDVTAEDKCHCCQFPAALRPHVVWFGEMPLGMDEIYSALADADIFIAIGTSGHVYPAAGFVHEARLHGAHTVEL
NLEPSQVGSEFAEKHYGLASEVVPAFIDKLL
>Mature_271_residues
MQSRRLHRLSRFRRNKRRLRDRLRQRIFFREDRMKPEAMAKPRVVVLTGAGISAESGIKTFRAADGLWEEHRVEDVATPE
GFARDPALVQAFYNARRRQLQSPEIAPNAAHLALARLEDLLGDHFLLVTQNIDNLHERAGNRRVIHMHGELLKVRCSWSG
QVLEWTGDVTAEDKCHCCQFPAALRPHVVWFGEMPLGMDEIYSALADADIFIAIGTSGHVYPAAGFVHEARLHGAHTVEL
NLEPSQVGSEFAEKHYGLASEVVPAFIDKLL

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=235, Percent_Identity=38.2978723404255, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI13787215, Length=236, Percent_Identity=37.2881355932203, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI6912664, Length=248, Percent_Identity=36.2903225806452, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI6912662, Length=259, Percent_Identity=30.1158301158301, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI7657575, Length=248, Percent_Identity=28.6290322580645, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI6912660, Length=207, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI308199517, Length=233, Percent_Identity=87.9828326180258, Blast_Score=430, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17541892, Length=203, Percent_Identity=32.512315270936, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17567771, Length=119, Percent_Identity=35.2941176470588, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571445, Length=128, Percent_Identity=38.28125, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 30668; Mature: 30668

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSRRLHRLSRFRRNKRRLRDRLRQRIFFREDRMKPEAMAKPRVVVLTGAGISAESGIKT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCHHHHHH
FRAADGLWEEHRVEDVATPEGFARDPALVQAFYNARRRQLQSPEIAPNAAHLALARLEDL
HHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGDHFLLVTQNIDNLHERAGNRRVIHMHGELLKVRCSWSGQVLEWTGDVTAEDKCHCCQF
HCCEEEEEECCHHHHHHHCCCCEEEEECCEEEEEEECCCCCEEEECCCCCCCCCCHHCCC
PAALRPHVVWFGEMPLGMDEIYSALADADIFIAIGTSGHVYPAAGFVHEARLHGAHTVEL
CCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCEECCHHHHHHHHHCCCEEEEE
NLEPSQVGSEFAEKHYGLASEVVPAFIDKLL
ECCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MQSRRLHRLSRFRRNKRRLRDRLRQRIFFREDRMKPEAMAKPRVVVLTGAGISAESGIKT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCHHHHHH
FRAADGLWEEHRVEDVATPEGFARDPALVQAFYNARRRQLQSPEIAPNAAHLALARLEDL
HHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGDHFLLVTQNIDNLHERAGNRRVIHMHGELLKVRCSWSGQVLEWTGDVTAEDKCHCCQF
HCCEEEEEECCHHHHHHHCCCCEEEEECCEEEEEEECCCCCEEEECCCCCCCCCCHHCCC
PAALRPHVVWFGEMPLGMDEIYSALADADIFIAIGTSGHVYPAAGFVHEARLHGAHTVEL
CCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCEECCHHHHHHHHHCCCEEEEE
NLEPSQVGSEFAEKHYGLASEVVPAFIDKLL
ECCHHHHHHHHHHHHCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]