Click here to switch to the map view.

The map label for this gene is nfsA [H]

Identifier: 152969439

GI number: 152969439

Start: 987646

End: 988368

Strand: Direct

Name: nfsA [H]

Synonym: KPN_00882

Alternate gene names: 152969439

Gene position: 987646-988368 (Clockwise)

Preceding gene: 152969438

Following gene: 152969440

Centisome position: 18.58

GC content: 62.52

Gene sequence:

>723_bases
ATGACGCCGACCATTGAGCTGCTGCGCAGCCACCGTTCTATCCGCCACTTCACCGATGCCCCCGTGAGCGACGAACAGCG
CGCAGAGATTATCGCCAGCGCGCAAGCGGCATCTACCTCGAGTTTTTTACAGTGCACCTCCATCATCCGTATTACTGACC
CGGCCCTGCGTGAACGTCTGGTCCCGCTGACCGGCGGCCAGCAGCACGTCGCCCAGGCAGCGGAGTTTTGGGTCTTCTGC
GCCGACTTTAATCGCCACCTGCAGATCTGCCCACAGGCGCAGCTGGGGCTGGCGGAACAGCTGCTGATCGGCGTTGTCGA
TACGGCGCTGCTGGCGCAAAACGCGTTGACCGCCGCCGAGTCCCTCGGACTAGGGGGCGTCTATATCGGCGGGTTGCGCA
ACAGCATTGAGGCCGTCACAGAGCTGCTGGAGCTGCCGCAGCACGTCCTGCCGCTGTTCGGTCTTTGCCTTGGCTGGCCG
GCGGATAACCCGGATATCAAGCCGCGGATGCCGGCGGCCATGCTGGTGCATGAGAACCGCTATCAGCCGCTGGATAACGC
GCTGCTGGCGGAGTATGACGAACAGCTGGCGCACTATTATCTGTCGCGCGGCAGCAACGCCCGCCGCGATACCTGGAGCG
ACCATATCCGGCGCACTATCGTGAACGAGAGTCGTCCGTTTATTCTTGACTATCTGCATAAGCAGGGGTGGGCAACGCGC
TGA

Upstream 100 bases:

>100_bases
CCCGCCGCCATCGTCGTGATGTGGCGGATGGCGCAGATCGTGGCCCCGCAGTTAACGCACTCTCCAAAGCCTGAATCCTT
GTCTGACAGAGAAAAACGAA

Downstream 100 bases:

>100_bases
TTACGTGTACACTGCTGCTCCGGCCAGGTGATATCCGCCGCCGGCCGCAGGAAAGGTGAAAGTGTGTTCGATAGCTGAGG
CCCGCCAGAGCGGGTCTTTT

Product: nitroreductase A

Products: NA

Alternate protein names: Modulator of drug activity A [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTPTIELLRSHRSIRHFTDAPVSDEQRAEIIASAQAASTSSFLQCTSIIRITDPALRERLVPLTGGQQHVAQAAEFWVFC
ADFNRHLQICPQAQLGLAEQLLIGVVDTALLAQNALTAAESLGLGGVYIGGLRNSIEAVTELLELPQHVLPLFGLCLGWP
ADNPDIKPRMPAAMLVHENRYQPLDNALLAEYDEQLAHYYLSRGSNARRDTWSDHIRRTIVNESRPFILDYLHKQGWATR

Sequences:

>Translated_240_residues
MTPTIELLRSHRSIRHFTDAPVSDEQRAEIIASAQAASTSSFLQCTSIIRITDPALRERLVPLTGGQQHVAQAAEFWVFC
ADFNRHLQICPQAQLGLAEQLLIGVVDTALLAQNALTAAESLGLGGVYIGGLRNSIEAVTELLELPQHVLPLFGLCLGWP
ADNPDIKPRMPAAMLVHENRYQPLDNALLAEYDEQLAHYYLSRGSNARRDTWSDHIRRTIVNESRPFILDYLHKQGWATR
>Mature_239_residues
TPTIELLRSHRSIRHFTDAPVSDEQRAEIIASAQAASTSSFLQCTSIIRITDPALRERLVPLTGGQQHVAQAAEFWVFCA
DFNRHLQICPQAQLGLAEQLLIGVVDTALLAQNALTAAESLGLGGVYIGGLRNSIEAVTELLELPQHVLPLFGLCLGWPA
DNPDIKPRMPAAMLVHENRYQPLDNALLAEYDEQLAHYYLSRGSNARRDTWSDHIRRTIVNESRPFILDYLHKQGWATR

Specific function: Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen- insensitive nitroreductase activity in E.coli [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=240, Percent_Identity=83.3333333333333, Blast_Score=406, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26770; Mature: 26639

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTIELLRSHRSIRHFTDAPVSDEQRAEIIASAQAASTSSFLQCTSIIRITDPALRERL
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
VPLTGGQQHVAQAAEFWVFCADFNRHLQICPQAQLGLAEQLLIGVVDTALLAQNALTAAE
CCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGLGGVYIGGLRNSIEAVTELLELPQHVLPLFGLCLGWPADNPDIKPRMPAAMLVHENR
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCC
YQPLDNALLAEYDEQLAHYYLSRGSNARRDTWSDHIRRTIVNESRPFILDYLHKQGWATR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
TPTIELLRSHRSIRHFTDAPVSDEQRAEIIASAQAASTSSFLQCTSIIRITDPALRERL
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
VPLTGGQQHVAQAAEFWVFCADFNRHLQICPQAQLGLAEQLLIGVVDTALLAQNALTAAE
CCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGLGGVYIGGLRNSIEAVTELLELPQHVLPLFGLCLGWPADNPDIKPRMPAAMLVHENR
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCC
YQPLDNALLAEYDEQLAHYYLSRGSNARRDTWSDHIRRTIVNESRPFILDYLHKQGWATR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568050; 8755878; 8905232; 9278503; 2570347; 9440535; 11034992 [H]