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The map label for this gene is cmr [H]

Identifier: 152969431

GI number: 152969431

Start: 980459

End: 981691

Strand: Direct

Name: cmr [H]

Synonym: KPN_00874

Alternate gene names: 152969431

Gene position: 980459-981691 (Clockwise)

Preceding gene: 152969428

Following gene: 152969434

Centisome position: 18.45

GC content: 60.26

Gene sequence:

>1233_bases
ATGCAAAATTATTCGCTTTCAGGCCGCCGCCTCGGAAGGCAGGCGCTGTTGTTCCCGTTATGTCTGGTGCTGTACGAATT
TTCCACTTATATCGGCAACGATATGATTCAGCCGGGCATGTTAGCCGTGGTGCAGGAATTTCAGGTGGGCAACGAATGGG
TACCTACCTCAATGACTGCCTATCTGGCAGGCGGCATGTTTTTACAGTGGCTGCTGGGGCCGCTGTCGGATCGTATTGGC
CGCCGTCCGGTGATGTTGACCGGCGTAGTATGGTTCATCGTTACCTGCCTGGCGACGCTGCTGGCGCAGACCATTGAGCA
GTTTACCCTGCTGCGCTTCCTGCAGGGGATCAGCCTGTGCTTTATTGGCGCGGTAGGCTATGCGGCGATACAGGAGTCCT
TTGAGGAGGCGGTGTGTATCAAAATCACCGCCCTGATGGCTAACGTGGCGCTGATTGCCCCGCTGCTGGGGCCGCTGGTG
GGCGCCGCCTGGGTGCATATCCTGCCGTGGGAGATGATGTTTGTCCTGTTCGCCGTGCTGGCGGCCATCTCGTTCTTCGG
CCTGCAGCGGGCGATGCCGGAGACCGCGACGCGGCTGGGCGAAAAGCTGTCGGTGAAAGAGCTGGGCCGCGACTATCGGC
TGGTGCTGAAGAACCTGCGCTTCGTCGCCGGCGCGCTGGCCACCGGCTTTGTCAGCCTGCCGCTGCTGGCGTGGATTGCC
CAGTCGCCGGTGATTATTATCAGCGGCGAACAGGCCACCAGCTATGAGTACGGCATGCTGCAGGTGCCGATTTTCGGGGC
GCTGATTGCCGGCAACCTGGTGCTGGCGCGCCTGACTGCGCGCCGGACCGTGCGCTCGCTGATTATCATGGGCGGTTGGC
CGATCATGTTTGGCCTGATCCTCTCTGCGGCGGCGACCGTGGTCTCTTCCCACGCTTATCTGTGGATGACGGCCGGTCTG
AGCTTCTACGCCTTCGGGATTGGCCTCGCGAACGCCGGCCTGGTGCGTTTAACCCTGTTCGCCAGCGAGATGAGTAAAGG
CACGGTCTCGGCGGCGATGGGAATGCTGCAGATGCTGATCTTCACCGTCGGCATCGAGCTCAGCAAACATGCTTACGAGC
TGGGCGGCAACGGGCTGTTCAGCCTGTTTAACCTGCTGGGTGGGGTACTATGGCTGGGGCTGATGATTTACTTTCTGAAA
GATAAAAGCGTCGGCAATTCGCAGCAGGGGTAG

Upstream 100 bases:

>100_bases
CGCACGGCTCGTAATATTGTGCGGTGTCACCCACAATTCACTATCTCCGCGCCGGGAGTTTCATTACACTTTGCGCGATT
TTTGATAGATAAGAGATTTC

Downstream 100 bases:

>100_bases
TTGTTTTCAACCCGGATGCGCTCGCATCCGGGTCTTATCAGGCGAACGGCGCTTCGCGATCCAACACCTTATCAATAATC
TGCAGGACACCTTCTTCGTT

Product: major facilitator superfamily multidrug/chloramphenicol efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQNYSLSGRRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVQEFQVGNEWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLTGVVWFIVTCLATLLAQTIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHILPWEMMFVLFAVLAAISFFGLQRAMPETATRLGEKLSVKELGRDYRLVLKNLRFVAGALATGFVSLPLLAWIA
QSPVIIISGEQATSYEYGMLQVPIFGALIAGNLVLARLTARRTVRSLIIMGGWPIMFGLILSAAATVVSSHAYLWMTAGL
SFYAFGIGLANAGLVRLTLFASEMSKGTVSAAMGMLQMLIFTVGIELSKHAYELGGNGLFSLFNLLGGVLWLGLMIYFLK
DKSVGNSQQG

Sequences:

>Translated_410_residues
MQNYSLSGRRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVQEFQVGNEWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLTGVVWFIVTCLATLLAQTIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHILPWEMMFVLFAVLAAISFFGLQRAMPETATRLGEKLSVKELGRDYRLVLKNLRFVAGALATGFVSLPLLAWIA
QSPVIIISGEQATSYEYGMLQVPIFGALIAGNLVLARLTARRTVRSLIIMGGWPIMFGLILSAAATVVSSHAYLWMTAGL
SFYAFGIGLANAGLVRLTLFASEMSKGTVSAAMGMLQMLIFTVGIELSKHAYELGGNGLFSLFNLLGGVLWLGLMIYFLK
DKSVGNSQQG
>Mature_410_residues
MQNYSLSGRRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVQEFQVGNEWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLTGVVWFIVTCLATLLAQTIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHILPWEMMFVLFAVLAAISFFGLQRAMPETATRLGEKLSVKELGRDYRLVLKNLRFVAGALATGFVSLPLLAWIA
QSPVIIISGEQATSYEYGMLQVPIFGALIAGNLVLARLTARRTVRSLIIMGGWPIMFGLILSAAATVVSSHAYLWMTAGL
SFYAFGIGLANAGLVRLTLFASEMSKGTVSAAMGMLQMLIFTVGIELSKHAYELGGNGLFSLFNLLGGVLWLGLMIYFLK
DKSVGNSQQG

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=410, Percent_Identity=87.5609756097561, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1790794, Length=385, Percent_Identity=42.0779220779221, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI48994889, Length=217, Percent_Identity=24.8847926267281, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788509, Length=253, Percent_Identity=23.7154150197628, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790146, Length=234, Percent_Identity=24.7863247863248, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082312, Length=161, Percent_Identity=31.6770186335404, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44539; Mature: 44539

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQNYSLSGRRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVQEFQVGNEWVPTSMTA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
YLAGGMFLQWLLGPLSDRIGRRPVMLTGVVWFIVTCLATLLAQTIEQFTLLRFLQGISLC
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWVHILPWEMMFVLFAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAISFFGLQRAMPETATRLGEKLSVKELGRDYRLVLKNLRFVAGALATGFVSLPLLAWIA
HHHHHHHHHHCCHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSPVIIISGEQATSYEYGMLQVPIFGALIAGNLVLARLTARRTVRSLIIMGGWPIMFGLI
CCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LSAAATVVSSHAYLWMTAGLSFYAFGIGLANAGLVRLTLFASEMSKGTVSAAMGMLQMLI
HHHHHHHHHCCHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
FTVGIELSKHAYELGGNGLFSLFNLLGGVLWLGLMIYFLKDKSVGNSQQG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]