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The map label for this gene is ybiK [H]

Identifier: 152969418

GI number: 152969418

Start: 963033

End: 963974

Strand: Direct

Name: ybiK [H]

Synonym: KPN_00861

Alternate gene names: 152969418

Gene position: 963033-963974 (Clockwise)

Preceding gene: 152969411

Following gene: 152969419

Centisome position: 18.12

GC content: 63.59

Gene sequence:

>942_bases
ATGGGCAAGGCGGTTATAGCAATTCACGGCGGCGCAGGGGCGATTTCTCGCGCGCAAATGACGCCGGAGCGGGAGCGAGA
GTATGTGGCGGCGCTGTCGACTATCGTCGAAAGCGGGCAGAAAATGTTGGCCGCGGGCGCCAGCGCGCTGGATGCCGTGG
CCGAGGCCGTTCGGCTGCTGGAGGAGTGTCCGTTGTTCAACGCCGGAATGGGCGCGGTATTTACCCGCGATCAAACCCAC
GAGCTGGACGCCTGCGTAATGGACGGCTACAGCCTGCAGGCTGGCGCGGTAGCCGGGGTCAAACATTTGCGTAATCCGGT
GCTGGCGGCGCGTCTGGTGCTGGAGAAGAGTCCGCACGTATTACTGATCGGCGAGGGGGCGGAAAATTTTGCCATCTCCC
ACGGGATGGCGCGCGTGGATAACGATCTCTTTTCCACTCCTGAAAGGCTGCTGCAGCTGCAGGAGGCGAAAGCGGGCGGG
GAGATTATCCTCGACCACCACGCCGCTCCCCTCGACGAACGCCAGAAAATGGGCACCGTCGGCGCGGTGGCGCTCGATCT
GGCCGGCAATCTGGCGGCGGCGACCTCCACCGGCGGCATGACCAACAAACTGCCCGGACGCGTCGGCGACAGCCCGCTGC
CGGGCGCGGGGTGTTATGCCAATAATGCCAGCGTGGCGGTCTCCTGTACCGGCACCGGCGAAGTCTTTATGCGCACCCTG
GCGGCCTACGATATCGCCGCCCTGATGGAGTATGGGCAGCTCAGTCTCTATTCCGCCTGCGAGCGGGTGGTGATGGAGAA
ACTGCCGGCTCTCGGCGGCAGCGGCGGGCTGATAGCCGTCGATCGCGAAGGCAACGTCGTTTTACCGTTTAACAGTGAAG
GCATGTACCGCGCCTGGTGCTATGCCGGCGATACGCCGACCATTGGCATTTATCGCGAATAG

Upstream 100 bases:

>100_bases
CAATATAATGATATCAATACCCTTATTCATGACGTGACCGGCGCCGCGCCGCGGTACGACAGGGCAGGGGCGATCCACTT
CATCGTTAAGGACTGGCGAA

Downstream 100 bases:

>100_bases
AACAAGGGAGAGGGCGAGTGTCGCAAACTCACGAAACCGATACCTGCGAAGTGCTGGTGGTGCGCAACCTGAACGTGGCG
TTCCGCCAGCAGGATGCGCC

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MGKAVIAIHGGAGAISRAQMTPEREREYVAALSTIVESGQKMLAAGASALDAVAEAVRLLEECPLFNAGMGAVFTRDQTH
ELDACVMDGYSLQAGAVAGVKHLRNPVLAARLVLEKSPHVLLIGEGAENFAISHGMARVDNDLFSTPERLLQLQEAKAGG
EIILDHHAAPLDERQKMGTVGAVALDLAGNLAAATSTGGMTNKLPGRVGDSPLPGAGCYANNASVAVSCTGTGEVFMRTL
AAYDIAALMEYGQLSLYSACERVVMEKLPALGGSGGLIAVDREGNVVLPFNSEGMYRAWCYAGDTPTIGIYRE

Sequences:

>Translated_313_residues
MGKAVIAIHGGAGAISRAQMTPEREREYVAALSTIVESGQKMLAAGASALDAVAEAVRLLEECPLFNAGMGAVFTRDQTH
ELDACVMDGYSLQAGAVAGVKHLRNPVLAARLVLEKSPHVLLIGEGAENFAISHGMARVDNDLFSTPERLLQLQEAKAGG
EIILDHHAAPLDERQKMGTVGAVALDLAGNLAAATSTGGMTNKLPGRVGDSPLPGAGCYANNASVAVSCTGTGEVFMRTL
AAYDIAALMEYGQLSLYSACERVVMEKLPALGGSGGLIAVDREGNVVLPFNSEGMYRAWCYAGDTPTIGIYRE
>Mature_312_residues
GKAVIAIHGGAGAISRAQMTPEREREYVAALSTIVESGQKMLAAGASALDAVAEAVRLLEECPLFNAGMGAVFTRDQTHE
LDACVMDGYSLQAGAVAGVKHLRNPVLAARLVLEKSPHVLLIGEGAENFAISHGMARVDNDLFSTPERLLQLQEAKAGGE
IILDHHAAPLDERQKMGTVGAVALDLAGNLAAATSTGGMTNKLPGRVGDSPLPGAGCYANNASVAVSCTGTGEVFMRTLA
AYDIAALMEYGQLSLYSACERVVMEKLPALGGSGGLIAVDREGNVVLPFNSEGMYRAWCYAGDTPTIGIYRE

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=301, Percent_Identity=39.5348837209302, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI145275200, Length=301, Percent_Identity=39.5348837209302, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI98991769, Length=273, Percent_Identity=34.4322344322344, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI285002251, Length=258, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI285002253, Length=258, Percent_Identity=31.3953488372093, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1787050, Length=313, Percent_Identity=80.5111821086262, Blast_Score=512, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17569239, Length=238, Percent_Identity=28.9915966386555, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32564649, Length=312, Percent_Identity=26.6025641025641, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI18921183, Length=320, Percent_Identity=35.9375, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=31.7164179104478, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI221330102, Length=251, Percent_Identity=33.4661354581673, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24652135, Length=235, Percent_Identity=34.0425531914894, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 32648; Mature: 32517

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKAVIAIHGGAGAISRAQMTPEREREYVAALSTIVESGQKMLAAGASALDAVAEAVRLL
CCCEEEEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHH
EECPLFNAGMGAVFTRDQTHELDACVMDGYSLQAGAVAGVKHLRNPVLAARLVLEKSPHV
HHCCCCCCCCCEEEECCCCCCCCHHEECCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCE
LLIGEGAENFAISHGMARVDNDLFSTPERLLQLQEAKAGGEIILDHHAAPLDERQKMGTV
EEEECCCCCHHHHCCHHHHCCHHHCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCH
GAVALDLAGNLAAATSTGGMTNKLPGRVGDSPLPGAGCYANNASVAVSCTGTGEVFMRTL
HHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCHHHHHHHH
AAYDIAALMEYGQLSLYSACERVVMEKLPALGGSGGLIAVDREGNVVLPFNSEGMYRAWC
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEEEE
YAGDTPTIGIYRE
EECCCCCEEEEEC
>Mature Secondary Structure 
GKAVIAIHGGAGAISRAQMTPEREREYVAALSTIVESGQKMLAAGASALDAVAEAVRLL
CCEEEEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHH
EECPLFNAGMGAVFTRDQTHELDACVMDGYSLQAGAVAGVKHLRNPVLAARLVLEKSPHV
HHCCCCCCCCCEEEECCCCCCCCHHEECCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCE
LLIGEGAENFAISHGMARVDNDLFSTPERLLQLQEAKAGGEIILDHHAAPLDERQKMGTV
EEEECCCCCHHHHCCHHHHCCHHHCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCH
GAVALDLAGNLAAATSTGGMTNKLPGRVGDSPLPGAGCYANNASVAVSCTGTGEVFMRTL
HHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCHHHHHHHH
AAYDIAALMEYGQLSLYSACERVVMEKLPALGGSGGLIAVDREGNVVLPFNSEGMYRAWC
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEEEE
YAGDTPTIGIYRE
EECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]