Click here to switch to the map view.

The map label for this gene is ddl

Identifier: 151222199

GI number: 151222199

Start: 2209236

End: 2210306

Strand: Reverse

Name: ddl

Synonym: NWMN_1987

Alternate gene names: 151222199

Gene position: 2210306-2209236 (Counterclockwise)

Preceding gene: 151222201

Following gene: 151222198

Centisome position: 76.78

GC content: 33.8

Gene sequence:

>1071_bases
ATGACAAAAGAAAATATTTGTATCGTTTTTGGAGGGAAAAGTGCAGAACACGAAGTATCGATTCTGACAGCACAAAATGT
ATTAAATGCAATAGATAAAGACAAATATCATGTTGATATCATTTATATTACCAATGATGGTGATTGGAGAAAGCAAAATA
ATATTACAGCTGAAATTAAATCTACTGATGAGCTTCATTTAGAAAATGGAGAGGCGCTTGAGATTTCACAGCTATTGAAA
GAAAGTAGTTCAGGACAACCATACGATGCAGTATTCCCATTATTACATGGTCCTAATGGTGAAGATGGCACGATTCAAGG
GCTTTTTGAAGTTTTGGATGTACCATATGTAGGAAATGGTGTATTGTCAGCTGCAAGTTCTATGGACAAACTTGTAATGA
AACAATTATTTGAACATCGAGGGTTACCACAGTTACCTTATATTAGTTTCTTACGTTCTGAATATGAAAAATATGAACAT
AACATTTTAAAATTAGTAAATGATAAATTAAATTACCCAGTCTTTGTTAAACCTGCTAACTTAGGGTCAAGTGTAGGTAT
CAGTAAATGTAATAATGAAGCGGAACTTAAAGAAGGTATTAAAGAAGCATTCCAATTTGACCGTAAGCTTGTTATAGAAC
AAGGCGTTAACGCACGTGAAATTGAAGTAGCAGTTTTAGGAAATGACTATCCTGAAGCGACATGGCCAGGTGAAGTCGTA
AAAGATGTCGCGTTTTACGATTACAAATCAAAATATAAAGATGGTAAGGTTCAATTACAAATTCCAGCTGACTTAGACGA
AGATGTTCAATTAACGCTTAGAAATATGGCATTAGAGGCATTCAAAGCGACAGATTGTTCTGGTTTAGTCCGTGCTGATT
TCTTTGTAACAGAAGACAACCAAATATATATTAATGAAACAAATGCAATGCCTGGATTTACGGCTTTCAGTATGTATCCA
AAGTTATGGGAAAATATGGGCTTATCTTATCCAGAATTGATTACAAAACTTATCGAGCTTGCTAAAGAACGTCACCAGGA
TAAACAGAAAAATAAATACAAAATTGACTAA

Upstream 100 bases:

>100_bases
AAGATTCAAAGATGCGAATAAATCAATTAACAATAGGACTAAATCAATATTAATTTATATTAAGGTAGCAAACCCTGATA
TATCATTGGAGGAAAACGAA

Downstream 100 bases:

>100_bases
CTGAGGTTGTTATTATGATTAATGTTACATTAAAGCAAATTCAATCATGGATTCCTTGTGAAATTGAAGATCAATTTTTA
AATCAAGAGATAAATGGAGT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYITNDGDWRKQNNITAEIKSTDELHLENGEALEISQLLK
ESSSGQPYDAVFPLLHGPNGEDGTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNAREIEVAVLGNDYPEATWPGEVV
KDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNMALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYP
KLWENMGLSYPELITKLIELAKERHQDKQKNKYKID

Sequences:

>Translated_356_residues
MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYITNDGDWRKQNNITAEIKSTDELHLENGEALEISQLLK
ESSSGQPYDAVFPLLHGPNGEDGTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNAREIEVAVLGNDYPEATWPGEVV
KDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNMALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYP
KLWENMGLSYPELITKLIELAKERHQDKQKNKYKID
>Mature_355_residues
TKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYITNDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKE
SSSGQPYDAVFPLLHGPNGEDGTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEHN
ILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNAREIEVAVLGNDYPEATWPGEVVK
DVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNMALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPK
LWENMGLSYPELITKLIELAKERHQDKQKNKYKID

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=358, Percent_Identity=42.7374301675978, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1786280, Length=341, Percent_Identity=32.8445747800587, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_STAA1 (A7X4R6)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442658.1
- ProteinModelPortal:   A7X4R6
- SMR:   A7X4R6
- STRING:   A7X4R6
- EnsemblBacteria:   EBSTAT00000002738
- GeneID:   5559426
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2068
- eggNOG:   COG1181
- GeneTree:   EBGT00050000025415
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   SAUR418127:SAHV_2068-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40231; Mature: 40100

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYITNDGDWRKQNNITAEIK
CCCCCEEEEECCCCCCCEEEEEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCEEEEEE
STDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGEDGTIQGLFEVLDVPYVGNG
CCCEEEECCCCEEHHHHHHHHCCCCCCHHHHHHHEECCCCCCCHHHHHHHHHCCCCCCCH
VLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEHNILKLVNDKLNYPVFVKPAN
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
LGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNAREIEVAVLGNDYPEATWPGEVV
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHH
KDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNMALEAFKATDCSGLVRADFFVTEDN
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCC
QIYINETNAMPGFTAFSMYPKLWENMGLSYPELITKLIELAKERHQDKQKNKYKID
EEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYITNDGDWRKQNNITAEIK
CCCCEEEEECCCCCCCEEEEEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCEEEEEE
STDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGEDGTIQGLFEVLDVPYVGNG
CCCEEEECCCCEEHHHHHHHHCCCCCCHHHHHHHEECCCCCCCHHHHHHHHHCCCCCCCH
VLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEHNILKLVNDKLNYPVFVKPAN
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
LGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNAREIEVAVLGNDYPEATWPGEVV
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHH
KDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNMALEAFKATDCSGLVRADFFVTEDN
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCC
QIYINETNAMPGFTAFSMYPKLWENMGLSYPELITKLIELAKERHQDKQKNKYKID
EEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA