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The map label for this gene is pnp [H]

Identifier: 150398632

GI number: 150398632

Start: 3653754

End: 3655898

Strand: Direct

Name: pnp [H]

Synonym: Smed_3446

Alternate gene names: 150398632

Gene position: 3653754-3655898 (Clockwise)

Preceding gene: 150398631

Following gene: 150398633

Centisome position: 96.61

GC content: 61.82

Gene sequence:

>2145_bases
ATGTTCGAGACTCACAAGGTAGAAATCGAATGGGCTGGGCGTCCGCTCAAGCTCGAAACCGGAAAGATCGCGCGTCAGGC
CGATGGTGCCGTTCTCGCGACCTACGGCGAAACCGTCGTGCTCGCCACGGTCGTTTCGGCCAAGGCACCGAAGCCCGGCC
AGGATTTCTTCCCGCTCACCGTCAACTACCAGGAAAAGACCTACGCTGCCGGCAAGATTCCGGGCGGCTATTTCAAGCGC
GAGGGCCGTCCCAGCGAAAACGAAACGCTGGTGTCCCGCCTGATCGACCGTCCGATCCGCCCGCTCTTCCCCGAAGGCTA
CAAGAATGACACCCAGGTCATCATCACGGTCATGCAGCATGATCTTGAGAACAACCCGGACGTCGTCGCCATGGTTGCAG
CTTCTGCTGCGTTGACGCTTTCCGGCGTTCCCTTCATGGGCCCGGTCGGTGGTGCCCGCGTCGGCTACATCAACGGCGAA
TACGTCCTCAACCCGCATCTCGACGAAATGGACGAGTCGACGCTCGACCTCGTCGTCGCCGGCACACAGGACGCCGTATT
GATGGTTGAATCCGAGGCCAAGGAGCTTTCGGAGGAGATCATGCTCGGCGCCGTCGTCTTCGGCCAGAAGGGCTTCCAGC
CGGTCATCGACGCAGTCATCAGGCTTGCCGAAGTCGCCGCCAAGGAGCCGCGCGAGTTCGAGCCGGACGACCATTCCGCT
CTCGAAAACGCCATGCTTTCGATCGCCGAAGAGGACCTGCGCAAGGCCTACAAGATCACCGAGAAGGCGCAGCGTTATGC
AGCCGTCGACGCGGTCAAGGCCAAGGTGAAGGAGCACTTCCTTCCGGAAGGCGTCGAGAACCCGGCTCATACGGCGGAAG
AGATCGCCTCGGTCTTCAAGCACCTGCAGGCCAAGATCGTTCGCTGGAACATCCTCGACACCAAGAGCCGCATCGACGGC
CGCGATCTCGTCACGGTTCGTCCGATCGTTGCCGAAGTCGGCCTCCTGCCGCGTACGCACGGTTCGGCGCTCTTCACCCG
CGGCGAGACTCAGGCGATCGTTGTCGCGACGCTCGGCACCGGAGAAGACGAGCAGTACGTCGATTCCTTGACAGGCATGT
ACAAGGAAAACTTCATGCTGCACTACAACTTCCCGCCCTACTCGGTCGGCGAAACGGGCCGCATGGGTTCCCCGGGTCGC
CGCGAGATCGGCCACGGCAAGCTTGCCTGGCGGGCAATCCATCCGATGCTGCCGACGGCGGAACAGTTCCCCTATACGCT
GCGGGTCGTTTCGGAAATCACCGAATCCAACGGCTCGTCCTCGATGGCGACCGTCTGCGGCACATCTCTCGCGCTGATGG
ATGCCGGCGTTCCGCTGGCCAAGCCCGTTGCCGGCATCGCCATGGGCCTGATCAAGGAGGACGACCGCTTCGCAGTCCTC
TCCGACATTCTCGGTGACGAGGATCATCTCGGCGACATGGACTTCAAGGTCGCAGGCACCGAAGCCGGCGTTACCTCGTT
GCAGATGGACATCAAGATTGCCGGCATCACCGAAGAGATCATGAAGGTCGCGCTCGACCAGGCGAAGCACGGTCGCGTTC
ACATTCTCGGCGAAATGGCCAAGGCGATCTCCGAGAGCCGTGGGCAGCTCGGCGAGTTCGCGCCGCGCATCGAAGTGATG
AACATTCCGGTCGACAAGATCCGCGAAGTCATCGGCTCGGGCGGCAAGGTCATCCGCGAAATCGTCGAGAAGACCGGTGC
CAAGATCAATATCGACGACGACGGCACGGTTAAGATCGCGTCGGCTTCCGGCAAGGAGATCGAGGCAGCCCGCAAGTGGA
TCCACTCGATCGTCGCCGAACCGGAAGTCGGCCAGGTCTATGAAGGCACCGTCGTCAAGACGGCCGATTTCGGCGCCTTC
GTGAACTTCTTCGGTGCACGCGATGGCCTCGTCCACATCTCTCAGCTCGCTTCCGAGCGCGTTGCCAAGACCACGGACGT
CGTCAAGGAAGGCGACAAGGTCTGGGTCAAGCTGATGGGCTTCGACGAGCGCGGCAAAGTGCGCCTTTCCATGAAGGTCG
TCGACCAGGCGACCGGCAAGGAGATCGCTGCCGAGAAGAAGGACGGCGGCGAAGCCGCCGAATAA

Upstream 100 bases:

>100_bases
AGCCTCCCGTTGTCTTGCCCGTGATGCGCCACCAGGCGGCTTGAGATCGCCCGCGCTTTTATCGAGCCGCGGAGGGTCTG
TCGCACACGAAGGACAGAAC

Downstream 100 bases:

>100_bases
ACCGGCCTCGGCTGAAACATTCAGGGCGCGGAGCGGATCGCTTCGCGCCCTTTCTTGTGACGATTGGATCGTCCCAGCAT
CGAAACAATGAAACGACGTA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MFETHKVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSENETLVSRLIDRPIRPLFPEGYKNDTQVIITVMQHDLENNPDVVAMVAASAALTLSGVPFMGPVGGARVGYINGE
YVLNPHLDEMDESTLDLVVAGTQDAVLMVESEAKELSEEIMLGAVVFGQKGFQPVIDAVIRLAEVAAKEPREFEPDDHSA
LENAMLSIAEEDLRKAYKITEKAQRYAAVDAVKAKVKEHFLPEGVENPAHTAEEIASVFKHLQAKIVRWNILDTKSRIDG
RDLVTVRPIVAEVGLLPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKENFMLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIHPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEDDRFAVL
SDILGDEDHLGDMDFKVAGTEAGVTSLQMDIKIAGITEEIMKVALDQAKHGRVHILGEMAKAISESRGQLGEFAPRIEVM
NIPVDKIREVIGSGGKVIREIVEKTGAKINIDDDGTVKIASASGKEIEAARKWIHSIVAEPEVGQVYEGTVVKTADFGAF
VNFFGARDGLVHISQLASERVAKTTDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIAAEKKDGGEAAE

Sequences:

>Translated_714_residues
MFETHKVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSENETLVSRLIDRPIRPLFPEGYKNDTQVIITVMQHDLENNPDVVAMVAASAALTLSGVPFMGPVGGARVGYINGE
YVLNPHLDEMDESTLDLVVAGTQDAVLMVESEAKELSEEIMLGAVVFGQKGFQPVIDAVIRLAEVAAKEPREFEPDDHSA
LENAMLSIAEEDLRKAYKITEKAQRYAAVDAVKAKVKEHFLPEGVENPAHTAEEIASVFKHLQAKIVRWNILDTKSRIDG
RDLVTVRPIVAEVGLLPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKENFMLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIHPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEDDRFAVL
SDILGDEDHLGDMDFKVAGTEAGVTSLQMDIKIAGITEEIMKVALDQAKHGRVHILGEMAKAISESRGQLGEFAPRIEVM
NIPVDKIREVIGSGGKVIREIVEKTGAKINIDDDGTVKIASASGKEIEAARKWIHSIVAEPEVGQVYEGTVVKTADFGAF
VNFFGARDGLVHISQLASERVAKTTDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIAAEKKDGGEAAE
>Mature_714_residues
MFETHKVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSENETLVSRLIDRPIRPLFPEGYKNDTQVIITVMQHDLENNPDVVAMVAASAALTLSGVPFMGPVGGARVGYINGE
YVLNPHLDEMDESTLDLVVAGTQDAVLMVESEAKELSEEIMLGAVVFGQKGFQPVIDAVIRLAEVAAKEPREFEPDDHSA
LENAMLSIAEEDLRKAYKITEKAQRYAAVDAVKAKVKEHFLPEGVENPAHTAEEIASVFKHLQAKIVRWNILDTKSRIDG
RDLVTVRPIVAEVGLLPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKENFMLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIHPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEDDRFAVL
SDILGDEDHLGDMDFKVAGTEAGVTSLQMDIKIAGITEEIMKVALDQAKHGRVHILGEMAKAISESRGQLGEFAPRIEVM
NIPVDKIREVIGSGGKVIREIVEKTGAKINIDDDGTVKIASASGKEIEAARKWIHSIVAEPEVGQVYEGTVVKTADFGAF
VNFFGARDGLVHISQLASERVAKTTDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIAAEKKDGGEAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=721, Percent_Identity=38.2801664355062, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI21361576, Length=86, Percent_Identity=46.5116279069767, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI4826690, Length=101, Percent_Identity=43.5643564356436, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI145693187, Length=686, Percent_Identity=57.4344023323615, Blast_Score=786, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=722, Percent_Identity=32.6869806094183, Blast_Score=348, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=90, Percent_Identity=44.4444444444444, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320850, Length=109, Percent_Identity=37.6146788990826, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281362905, Length=719, Percent_Identity=37.413073713491, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=719, Percent_Identity=37.413073713491, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=719, Percent_Identity=37.413073713491, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=659, Percent_Identity=37.1775417298938, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=45.0549450549451, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77456; Mature: 77456

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFETHKVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLT
CCCCEEEEEEECCCEEEECCCCHHHHCCCCEEEECCCEEEEEEHHHCCCCCCCCCCEEEE
VNYQEKTYAAGKIPGGYFKREGRPSENETLVSRLIDRPIRPLFPEGYKNDTQVIITVMQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEHHH
DLENNPDVVAMVAASAALTLSGVPFMGPVGGARVGYINGEYVLNPHLDEMDESTLDLVVA
CCCCCCCEEEEEEHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCEEEEEE
GTQDAVLMVESEAKELSEEIMLGAVVFGQKGFQPVIDAVIRLAEVAAKEPREFEPDDHSA
CCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
LENAMLSIAEEDLRKAYKITEKAQRYAAVDAVKAKVKEHFLPEGVENPAHTAEEIASVFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HLQAKIVRWNILDTKSRIDGRDLVTVRPIVAEVGLLPRTHGSALFTRGETQAIVVATLGT
HHHHHEEEEEEECCCCCCCCCCEEEEHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECC
GEDEQYVDSLTGMYKENFMLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIHPMLPTA
CCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCH
EQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEDDRFAVL
HHCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHH
SDILGDEDHLGDMDFKVAGTEAGVTSLQMDIKIAGITEEIMKVALDQAKHGRVHILGEMA
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCCCEEEHHHHH
KAISESRGQLGEFAPRIEVMNIPVDKIREVIGSGGKVIREIVEKTGAKINIDDDGTVKIA
HHHHHHHCCHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEEE
SASGKEIEAARKWIHSIVAEPEVGQVYEGTVVKTADFGAFVNFFGARDGLVHISQLASER
CCCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHH
VAKTTDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIAAEKKDGGEAAE
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEHHHHCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MFETHKVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLT
CCCCEEEEEEECCCEEEECCCCHHHHCCCCEEEECCCEEEEEEHHHCCCCCCCCCCEEEE
VNYQEKTYAAGKIPGGYFKREGRPSENETLVSRLIDRPIRPLFPEGYKNDTQVIITVMQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEHHH
DLENNPDVVAMVAASAALTLSGVPFMGPVGGARVGYINGEYVLNPHLDEMDESTLDLVVA
CCCCCCCEEEEEEHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCEEEEEE
GTQDAVLMVESEAKELSEEIMLGAVVFGQKGFQPVIDAVIRLAEVAAKEPREFEPDDHSA
CCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
LENAMLSIAEEDLRKAYKITEKAQRYAAVDAVKAKVKEHFLPEGVENPAHTAEEIASVFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HLQAKIVRWNILDTKSRIDGRDLVTVRPIVAEVGLLPRTHGSALFTRGETQAIVVATLGT
HHHHHEEEEEEECCCCCCCCCCEEEEHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECC
GEDEQYVDSLTGMYKENFMLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIHPMLPTA
CCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCH
EQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEDDRFAVL
HHCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHH
SDILGDEDHLGDMDFKVAGTEAGVTSLQMDIKIAGITEEIMKVALDQAKHGRVHILGEMA
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCCCEEEHHHHH
KAISESRGQLGEFAPRIEVMNIPVDKIREVIGSGGKVIREIVEKTGAKINIDDDGTVKIA
HHHHHHHCCHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEEE
SASGKEIEAARKWIHSIVAEPEVGQVYEGTVVKTADFGAFVNFFGARDGLVHISQLASER
CCCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHH
VAKTTDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIAAEKKDGGEAAE
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEHHHHCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]